An airborne biological particle monitoring device

Abstract

This invention relates to the field of microbial detection technology, specifically to an airborne biological particle monitoring device including a processing chamber and a pretreatment unit. The pretreatment unit includes a partition installed within the processing chamber, with two spaces on either side of the partition alternately performing filtration. A sterilization unit includes a first gas collecting pipe installed at one end of the processing chamber, connected to a first and a second air inlet pipe, with a sterilization chamber mounted on the first air inlet pipe. A fluorescent particle counting unit includes a second gas collecting pipe connected to both the first and second air inlet pipes, connected to a counting chamber containing an optical counting component. By using the pretreatment unit to filter out large particles from the airflow, and dividing the processing chamber into two spaces that operate alternately and self-clean, the fluorescent particle counting unit calculates the number of active biological aerosol particles. The sterilization unit provides a comparative test, improving the accuracy of the detection.

Description

Technical Field

[0001] This invention relates to the field of microbial detection technology, specifically to an airborne biological particle monitoring device. Background Technology

[0002] Real-time monitoring of bioaerosols has significant application value. Excitation-induced intrinsic fluorescence detection technology has significant advantages in the field of real-time bioaerosol monitoring due to its advantages such as high speed, high sensitivity, no consumables, and non-invasiveness. The technical principle is that bioaerosol particles contain organic molecules such as tryptophan, reduced coenzyme I (NADH), and riboflavin. These components can produce intrinsic fluorescence under ultraviolet light induction, while non-bioaerosol particles generally do not produce intrinsic fluorescence. Therefore, it is possible to distinguish whether aerosol particles possess biological properties.

[0003] However, existing technologies currently have the following main problems: First, airborne particles such as cigarette smoke particles, kaolin, and dust containing polycyclic aromatic hydrocarbons can also produce intrinsic fluorescence signals. Most existing technologies cannot distinguish these interfering substances, which may cause false detections or false alarms by the instruments. Second, there are many types of bioaerosol particles. In practical applications, it is often only necessary to monitor a few or a dozen biological targets. However, most existing technologies can only monitor the total number of fluorescent particles in general and cannot classify aerosol particles. For example, pollen or some harmless microorganisms do not need to be monitored. Therefore, it is necessary to conduct preliminary screening of biological particles to reduce the frequency and cost of subsequent biological detection.

[0004] Secondly, large particles in the airflow can also affect the test results during the detection process. Summary of the Invention

[0005] In view of the above-mentioned shortcomings of the existing technology, the present invention provides an airborne biological particle monitoring device that can effectively solve the problems of low detection efficiency, high cost and large particles affecting the detection results of the existing technology.

[0006] To achieve the above objectives, the present invention provides the following technical solution: This invention provides an airborne biological particle monitoring device, including a processing box, and further comprising: A pretreatment unit for filtering air includes a partition installed in a treatment chamber, wherein two spaces above and below the partition alternately perform filtration, and an aerosol gas flow passes through the treatment chamber. The sterilization unit includes a first gas collection pipe installed at one end of the processing box. The first gas collection pipe is connected to a first air inlet pipe and a second air inlet pipe respectively. A sterilization box is provided on the first air inlet pipe. The fluorescent particle counting unit includes a second gas collecting pipe connected to both the first and second gas inlets. The second gas collecting pipe is connected to a counting box, which contains an optical counting component. Furthermore, a second valve is provided on the first intake pipe, and a first valve is provided on the second intake pipe.

[0007] Furthermore, the processing box is equipped with two filters, which are located on the upper and lower sides of the partition, respectively. Both the upper and lower sides of the partition are equipped with louvers, and the outer side of the processing box is equipped with two switches for adjusting the opening and closing of the louvers. An adjustment box is provided on one side of the processing box, and a sliding slider is installed in the adjustment box. When there is airflow in the processing box, the slider slides up and down repeatedly, and when the slider slides back and forth, it intermittently triggers the two opening and closing switches.

[0008] Furthermore, a rotating rod is rotatably inserted at the top of the first gas collecting pipe, and a fan wheel is installed at one end of the rotating rod located in the first gas collecting pipe. A first transmission wheel is fixedly installed at the top of the rotating rod. A reciprocating screw is rotatably installed in the regulating box. The top of the reciprocating screw rotatably passes through the regulating box and is fixedly installed with a second transmission wheel. A transmission belt is provided between the first transmission wheel and the second transmission wheel.

[0009] Furthermore, two cleaning racks are slidably installed in the processing box, and the two cleaning racks are at right angles. Collection boxes are provided on the top and bottom walls of the processing box, and the two cleaning racks slide up and down synchronously with the slider.

[0010] Furthermore, a first magnet is fixedly installed on the side wall of the slider, a connecting rod is fixedly installed on the bottom wall of the first magnet, a second magnet is fixedly installed at the bottom end of the connecting rod, and permanent magnets that attract the first magnet and the second magnet are respectively provided on the side walls of the two cleaning racks.

[0011] Furthermore, both collection boxes are equipped with trapezoidal tubes, and negative pressure airflow is generated in the collection boxes when the cleaning rack moves to the collection box.

[0012] Furthermore, a piston tube is fixedly installed on the side wall of the processing box, a piston rod is movably inserted into the top of the piston tube, the top end of the piston rod is fixedly connected to a connecting rod, a first suction pipe is connected between the piston tube and one of the collection boxes, and a second suction pipe is connected between the piston tube and the other collection box.

[0013] Furthermore, the optical counting assembly includes a laser emitting device installed in a counting box, and the counting box is also equipped with a scattered light photomultiplier tube and a fluorescent photomultiplier tube.

[0014] Furthermore, the sterilization chamber is equipped with a sterilization device, which uses an ultraviolet lamp.

[0015] The technical solution provided by this invention has the following advantages compared with the known prior art: Large particles in the airflow are removed by setting up a pretreatment unit, and the treatment chamber is divided into two spaces that work alternately and achieve self-cleaning. In addition, the number of active bioaerosol particles is calculated by a fluorescent particle counting unit, and a sterilization unit is set up to form a comparative experiment, thereby improving the accuracy of detection. Attached Figure Description

[0016] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the accompanying drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are merely some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without any creative effort.

[0017] Figure 1 This is an overall schematic diagram of the present invention; Figure 2 A schematic diagram of the structure of the adjustment component; Figure 3 This is a schematic diagram of the internal structure of the processing box; Figure 4 for Figure 3 Enlarged view of the structure of part A in the middle; Figure 5 This is a schematic diagram of the fluorescent particle counting unit. Figure 6 This is a flowchart illustrating the process of airborne microbial detection.

[0018] The labels in the diagram represent: 1. Processing box; 2. First gas collection pipe; 3. First air inlet pipe; 4. Second air inlet pipe; 5. Second gas collection pipe; 6. Counting box; 7. Sterilization box; 8. First valve; 9. Second valve; 10. Partition; 11. Filter screen; 12. Louver; 13. Collection box; 14. Fan wheel; 15. Reciprocating screw; 16. First drive wheel; 17. Second drive wheel; 18. Drive belt; 19. Sliding slider; 20. First magnet; 21. Second magnet; 22. Connecting rod; 23. Piston tube; 24. Piston rod; 25. First suction pipe; 26. First exhaust pipe; 27. Second suction pipe; 28. Second exhaust pipe; 29. ​​Adjustment box; 30. Trapezoidal tube; 31. Cleaning rack; 32. Rotating rod. Detailed Implementation

[0019] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of the present invention. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are within the scope of protection of the present invention.

[0020] The present invention will be further described below with reference to embodiments.

[0021] Example 1: refer to Figure 1 as well as Figure 6 The flowchart shows an airborne biological particle monitoring device, which includes a processing box 1 and a sterilization unit. The device includes a first gas collecting pipe 2 installed at one end of the processing box 1. The first gas collecting pipe 2 is connected to a first air inlet pipe 3 and a second air inlet pipe 4. The first air inlet pipe 3 is provided with a second valve 9, the second air inlet pipe 4 is provided with a first valve 8, and the first air inlet pipe 3 is provided with a sterilization box 7.

[0022] The fluorescent particle counting unit includes a second gas collecting pipe 5 connected to both the first air inlet pipe 3 and the second air inlet pipe 4. The second air collecting pipe 5 is connected to a counting box 6, which contains an optical counting component. The optical counting component includes a laser emitting device installed in the counting box 6. The counting box 6 also contains a scattered light photomultiplier tube and a fluorescent photomultiplier tube. The sterilization box 7 is equipped with a sterilization device that uses an ultraviolet lamp, which can inactivate bacteria without producing other particles.

[0023] First, the airflow undergoes pretreatment to remove large particles. Then, the first valve 8 is opened and the second valve 9 is closed, allowing the airflow to pass directly through the fluorescent particle counting unit for one counting cycle, obtaining the total number of particles and the number of fluorescent particles. If the number of fluorescent particles is extremely low or zero, it can be determined that there are no biological aerosol particles. When the fluorescent particle count is large or significantly increased, after the value stabilizes, the total particle count TN1 and the number of fluorescent particles FN1 are recorded. Then, the first valve 8 is closed and the second valve 9 is opened, allowing the aerosol particles to pass through the sterilization unit and then enter the fluorescent particle counting unit for one counting cycle, obtaining the total particle count TN2 and the number of fluorescent particles FN2. TN1 and TN2 are not significantly different. If TN1 / TN2-1 does not exceed ±k, then FN1 / FN2-1 > k, indicating that active biological aerosol particles are present or have increased in the inhaled aerosol sample.

[0024] Example 2: refer to Figure 2 and Figure 3To improve detection accuracy, a pretreatment unit is installed in the processing box 1 to filter the air and remove large particles from the airflow. This unit includes a partition 10 installed in the processing box 1. The upper and lower spaces of the partition 10 alternately perform filtration. Aerosol airflow flows through the processing box 1. The processing box 1 is equipped with two filters 11, which are located on the upper and lower sides of the partition 10, respectively. Both the upper and lower sides of the partition 10 are equipped with louvers 12. Two switches for adjusting the opening and closing of the louvers 12 are provided on the outside of the processing box 1. An adjustment box 29 is provided on one side of the processing box 1. A sliding slider 19 is installed in the adjustment box 29. When there is airflow in the processing box 1, the slider 19 slides up and down repeatedly. When the slider 19 slides back and forth, it intermittently triggers the two opening and closing switches.

[0025] The processing chamber 1 is divided into two independent spaces by a partition 10, and each space is equipped with an independent filter 11 and louvers 12. The two louvers 12 are opened and closed alternately by adjusting the slider 19 in the adjustment chamber 29, that is, the two louvers 12 are opened alternately, and the filters 11 in the two spaces work alternately. And when one filter 11 is working, the other filter 11 performs self-cleaning.

[0026] like Figure 2 As shown, the specific alternating operation and self-cleaning process are achieved through the following components: a rotating rod 32 is rotatably inserted into the top of the first gas collecting pipe 2, and a fan wheel 14 is installed at one end of the rotating rod 32 located in the first gas collecting pipe 2. A first transmission wheel 16 is fixedly installed at the top of the rotating rod 32. A reciprocating screw 16 is rotatably installed in the regulating box 29. The top of the reciprocating screw 15 rotatably passes through the regulating box 29 and is fixedly installed with a second transmission wheel 17 (e.g., ...). Figure 2 (See the transmission diagram shown). A transmission belt 18 is provided between the first transmission wheel 16 and the second transmission wheel 17. Two cleaning racks 31 are slidably installed in the processing box 1. The two cleaning racks 31 are at right angles. Collection boxes 13 are provided on the top and bottom walls of the processing box 1. The two cleaning racks 31 slide up and down synchronously with the slider 19. A first magnet 20 is fixedly installed on the side wall of the slider 19. A connecting rod 22 is fixedly installed on the bottom wall of the first magnet 20. A second magnet 21 is fixedly installed at the bottom end of the connecting rod 22. Permanent magnets that attract the first magnet 20 and the second magnet 21 are respectively provided on the side walls of the two cleaning racks 31.

[0027] Combination Figure 2 and Figure 4Both collection boxes 13 are equipped with trapezoidal tubes 30. When the cleaning rack 31 moves up to the collection box 13, a negative pressure airflow is generated in the collection box 13. A piston tube 23 is fixedly installed on the side wall of the treatment box 1. A piston rod 24 is movably inserted into the top of the piston tube 23. The top of the piston rod 24 is fixedly connected to the connecting rod 22. A first suction pipe 25 is connected between the piston tube 23 and one of the collection boxes 13. A second suction pipe 27 is connected between the piston tube 23 and the other collection box 13. A first exhaust pipe 26 and a second exhaust pipe 28 are also provided on one side of the piston tube 23. One-way valves are respectively provided on the first suction pipe 25, the first exhaust pipe 26, the second suction pipe 27 and the second exhaust pipe 28.

[0028] The two spaces work alternately, but there is always airflow in the first air collection pipe 2. The airflow drives the impeller 14 to rotate, and the impeller 14 drives the rotating rod 32 to rotate. Through the transmission action between the first transmission wheel 16 and the second transmission wheel 17, the reciprocating screw 15 is driven to rotate (the transmission ratio can be changed according to the radius ratio of the two transmission wheels, thereby changing the rotation speed of the reciprocating screw 15). During the rotation of the reciprocating screw 15, the slider 19 will slide up and down in the regulating box 29.

[0029] As the slider 19 slides up and down, it causes the first magnet 20 and the second magnet 21 to slide up and down together, driving the two cleaning racks 31 to slide up and down together through magnetic force. The cleaning racks 31 clean the filter screen 11 by sliding. Specifically, when the cleaning rack 31 moves upward, it can clean the filter screen 11 in the upper part of the space. When the cleaning rack 31 rises to the top of the processing box 1, it pushes the collected large particles into the top collection box 13. When the cleaning rack 31 moves downward, it can clean the filter screen 11 in the lower part of the space. When the cleaning rack 31 descends to the bottom of the processing box 1, it pushes the collected large particles into the bottom collection box 13.

[0030] The bottom collection box 13 can collect large particles by gravity. To ensure the top collection box 13 can also collect large particles effectively, when the cleaning rack 31 moves to the collection box 13, a negative pressure airflow is generated within it, drawing out the large particles from the cleaning rack 31. Figure 4 As shown, a trapezoidal tube 30 is installed in the collection box 13 to prevent large particles from flowing back to the filter screen 11.

[0031] As the first magnet 20 and the second magnet 21 move upwards, i.e., when the cleaning rack 31 is driven upwards by magnetic force, the piston rod 24 moves outwards from the piston tube 23. The space at the bottom of the piston tube 23 continuously increases, thereby drawing air from the top collection box 13 through the second suction pipe 27. To ensure that the cleaning rack 31 only draws air when it reaches the collection box 13, control valves can be installed on the first suction pipe 25 and the second suction pipe 27. When the cleaning rack 31 moves to the collection box 13, the control valves are opened. At this time, the cleaning rack 31 is very close to the collection box 13, and large particles are drawn away by the airflow. Similarly, the bottom collection box 13 is also treated the same way, using airflow to draw away large particles. It is worth noting that when the cleaning rack 31 moves upwards to the top, the upper louver 12 is closed and the lower louver 12 is open. Conversely, when the cleaning rack 31 moves downwards to the bottom, the upper louver 12 is open and the lower louver 12 is closed. This alternating operation ensures a good pretreatment effect.

[0032] The above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit it. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions will not cause the essence of the corresponding technical solutions to deviate from the protection scope of the technical solutions of the embodiments of the present invention.

Claims

1. An airborne biological particle monitoring device, comprising a processing chamber, characterized in that, Also includes: A pretreatment unit for filtering air includes a partition installed in a treatment chamber. The upper and lower spaces of the partition alternately perform filtration. An aerosol airflow passes through the treatment chamber. The treatment chamber is equipped with two filters, which are located on the upper and lower sides of the partition, respectively. Both the upper and lower sides of the partition are equipped with louvers. Two switches for adjusting the opening and closing of the louvers are respectively provided on the outside of the treatment chamber. An adjustment box is provided on one side of the treatment chamber. A sliding slider is installed in the adjustment box. When there is airflow in the treatment chamber, the slider slides up and down repeatedly. When the slider slides back and forth, it intermittently triggers the two opening and closing switches. The sterilization unit includes a first gas collection pipe installed at one end of the processing box. The first gas collection pipe is connected to a first air inlet pipe and a second air inlet pipe respectively. A sterilization box is provided on the first air inlet pipe. A rotating rod is rotatably inserted at the top of the first gas collecting pipe, and a fan wheel is installed at one end of the rotating rod in the first gas collecting pipe. A first transmission wheel is fixedly installed at the top of the rotating rod. A reciprocating screw is rotatably installed in the regulating box. The top of the reciprocating screw rotatably passes through the regulating box and is fixedly installed with a second transmission wheel. A transmission belt is provided between the first transmission wheel and the second transmission wheel. Two cleaning racks are slidably installed in the processing box. The two cleaning racks slide up and down synchronously with the slider. A first magnet is fixedly installed on the side wall of the slider. A connecting rod is fixedly installed on the bottom wall of the first magnet. A second magnet is fixedly installed at the bottom end of the connecting rod. Permanent magnets that attract the first magnet and the second magnet are respectively provided on the side walls of the two cleaning racks. Both collection boxes are equipped with trapezoidal tubes, and negative pressure airflow is generated in the collection boxes when the cleaning rack moves to the collection box. The fluorescent particle counting unit includes a second gas collecting pipe connected to both the first and second gas inlet pipes. The second gas collecting pipe is connected to a counting box, which contains an optical counting component.

2. The airborne biological particle monitoring device according to claim 1, characterized in that, The first intake pipe is equipped with a second valve, and the second intake pipe is equipped with a first valve.

3. The airborne biological particle monitoring device according to claim 1, characterized in that, The two cleaning racks are at right angles, and collection boxes are provided on the top and bottom walls of the processing box.

4. The airborne biological particle monitoring device according to claim 1, characterized in that, A piston tube is fixedly installed on the side wall of the processing box. A piston rod is movably inserted into the top of the piston tube. The top end of the piston rod is fixedly connected to a connecting rod. A first suction pipe is connected between the piston tube and one of the collection boxes, and a second suction pipe is connected between the piston tube and the other collection box.

5. The airborne biological particle monitoring device according to claim 1, characterized in that, The optical counting assembly includes a laser emitting device installed in a counting box, which also contains a scattered light photomultiplier tube and a fluorescent photomultiplier tube.

6. The airborne biological particle monitoring device according to claim 1, characterized in that, The sterilization chamber is equipped with sterilization equipment, which uses ultraviolet lamps.

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