A biogas slurry nutrient synergist for corn and a preparation method thereof

Abstract

The application discloses a biogas slurry nutrient synergist for corn and a preparation method thereof, and relates to the technical field of organic fertilizer and microbial fertilizer manufacturing.The biogas slurry nutrient synergist for corn is prepared from the following raw materials in parts by mass: humic acid 10-15 parts, dicyanediamine 7-10 parts, a high molecular material 3-5 parts, a trace element fertilizer 0.5-2 parts, a compound microbial agent 5-8 parts, a suspending agent 10-15 parts, a plant growth regulator 0.5-2 parts and diatomite 40-65 parts.Through specific plant growth regulators and an optimized synergist formula and preparation method, the nutrient release in the biogas slurry can be more balanced and stable, nutrient loss can be reduced, efficient utilization can be achieved, and the yield and quality of corn are improved.

Description

Technical Field

[0001] This invention relates to the field of organic fertilizer and microbial fertilizer manufacturing technology, specifically to a biogas slurry nutrient enhancer for corn and its preparation method. Background Technology

[0002] In agricultural production, corn, as an important food crop, has always been a key focus of scientific research and production in terms of yield and quality improvement. Traditional fertilization methods mainly rely on chemical fertilizers and organic fertilizers. However, long-term use of chemical fertilizers can lead to problems such as soil compaction, decreased fertility, and environmental pollution. While organic fertilizers can improve soil structure, their nutrient release is slow and cannot meet the rapid demand of corn during its peak growth period.

[0003] Biogas slurry, as an inexpensive and nutrient-rich organic fertilizer source, has broad application prospects. However, the nutrient content of biogas slurry is unstable and it may contain pathogens and other adverse factors, resulting in limited effectiveness when applied directly and making it difficult to fully realize its fertilizer efficiency. In addition, existing fertilizer synergists have many shortcomings in improving the nutrient utilization efficiency of biogas slurry, such as insignificant effects, unbalanced composition, and poor soil compatibility.

[0004] Therefore, there is an urgent need to develop a nutrient enhancer for maize biogas slurry that is scientifically formulated, easy to prepare, and capable of achieving multiple synergistic effects, in order to break through existing technological bottlenecks, improve the fertilizer efficiency of biogas slurry and the production benefits of maize, and promote the sustainable development of agricultural waste resource utilization. Summary of the Invention

[0005] The purpose of this invention is to address the problems of low nutrient utilization efficiency, poor stability, and insufficient soil compatibility in existing biogas slurry technologies by providing a biogas slurry nutrient enhancer specifically for maize and its preparation method. This enhancer aims to achieve a multi-effect synergistic improvement in biogas slurry nutrients, including stabilizing nutrient release, enhancing microbial activity, and ultimately increasing maize yield and quality.

[0006] To achieve the above objectives, the technical solution adopted by the present invention is: a nutrient enhancer for maize biogas slurry, composed of the following raw materials in parts by weight: humic acid: 10-15 parts, dicyandiamine: 7-10 parts, polymeric material: 3-5 parts, trace element fertilizer: 0.5-2 parts, compound microbial agent: 5-8 parts, suspending agent: 10-15 parts, plant growth regulator: 0.5-2 parts, diatomaceous earth: 40-65 parts;

[0007] The plant growth regulator is a compound represented by Formula 1:

[0008] Formula 1:

[0009] R1 in Formula 1 is a substituent;

[0010] R1 is methyl, ethyl, methoxy, nitro, or phenyl.

[0011] Furthermore, the humic acid is at least one of fulvic acid or nitrohumic acid.

[0012] Furthermore, the polymer material is at least one of polyethylene glycol or sodium alginate.

[0013] Furthermore, the micronutrient fertilizer is composed of EDTA chelated iron, EDTA chelated zinc, EDTA chelated manganese, and EDTA chelated copper in a mass ratio of 5:2:2:1.

[0014] Furthermore, the compound microbial agent is composed of Bacillus amyloliquefaciens, Bacillus amyloliquefaciens and Bacillus subtilis mixed in a mass ratio of 2:1:1, and the bacterial content of each agent is ≥10 billion CFU / g.

[0015] Furthermore, the suspending agent is at least one of dehydrated sorbitan fatty acid ester or hydroxypropyl cellulose.

[0016] Furthermore, the plant growth regulator is any one of the compounds shown in the following structures:

[0017]

[0018]

[0019] A method for preparing a nutrient enhancer for maize biogas slurry includes the following steps:

[0020] S1. The Bacillus amyloliquefaciens, Bacillus amyloliquefaciens and Bacillus subtilis are subjected to liquid deep fermentation. After the spore formation rate is greater than 95%, they are mixed with zeolite that has passed through a 60-mesh sieve at a weight ratio of 1:4. After drying at 30°C, they are passed through a 40-mesh sieve to obtain each solid microbial agent. They are then mixed in proportion to prepare a compound microbial agent.

[0021] S2. Place the humic acid, dicyandiamine, polymer material, suspending agent and diatomaceous earth in a stirrer and stir at 200-400 rpm for 15-30 minutes to make them fully mixed to obtain mixture A;

[0022] S3. Place the micronutrient fertilizer, compound microbial agent and plant growth regulator in a mixer and stir at 200-400 rpm for 15-30 minutes to ensure thorough mixing and obtain mixture B;

[0023] S4. Add the mixture B to the mixture A, stir and mix at 25-45℃ for 10-20 minutes to disperse evenly and obtain a corn biogas slurry nutrient enhancer.

[0024] Furthermore, the culture medium for deep fermentation in S1 consists of 20-24 g / L yeast powder, 18-24 g / L soybean meal, and 0.8-8.5 g / L K2HPO4, with the pH adjusted to 7.0-7.6, the culture temperature at 28±2℃, and the culture time at 72-96 hours.

[0025] A method for using a biogas slurry nutrient enhancer for corn involves mixing the enhancer with biogas slurry at a mass ratio of 1:(100-200), stirring evenly, and then allowing it to stand for 2-4 hours to mature. Subsequently, it can be applied through irrigation or foliar spraying.

[0026] The plant growth regulator molecules described in this invention can bind to hormone receptors within plants, promoting cell division and elongation. By mimicking the action of auxin, it stimulates the division and elongation of cells in the roots and stems of maize, thereby establishing a stronger root system (enhancing nutrient absorption capacity) and more vigorous above-ground parts (enhancing photosynthesis). It can also regulate the growth and development cycle, participating in the transition of maize from vegetative to reproductive growth, promoting ear differentiation and grain filling, ultimately increasing yield, enhancing plant stress resistance, and activating systemic resistance, thus strengthening maize's resistance to pathogens and drought stress. Furthermore, it improves nutrient absorption and transport efficiency by affecting the permeability of plant cell membranes and regulating the activity of carrier proteins responsible for nutrient transport, thereby promoting the absorption, transport, and distribution of nitrogen, phosphorus, potassium, and trace elements from biogas slurry within maize. This regulator, as a bioactive signaling molecule, directly stimulates the growth and development of maize by mimicking endogenous hormones, enhancing stress resistance, and regulating the transport system. It also produces a strong synergistic effect with other components (humic acid, microbial agents, and trace elements), ultimately enhancing the nutrient content of biogas slurry.

[0027] The synergist described in this invention constructs a multifunctional system through the precise proportioning and synergistic effect of its components, jointly achieving the comprehensive goal of "strengthening plants and increasing yield." Diatomaceous earth, with its porous structure, provides a huge specific surface area. It first acts as a carrier, adsorbing and fixing free nutrients (such as ammonium nitrogen) in the biogas slurry, preventing rapid nutrient loss or leaching, thus achieving slow release and stability of nutrients. The suspending agent ensures that the synergist forms a stable suspension when mixed with the biogas slurry. Dicyandiamine effectively inhibits the activity of nitrifying bacteria in the soil, preventing the rapid oxidation of ammonium nitrogen in the biogas slurry into nitrate nitrogen, thereby significantly reducing nitrogen loss due to leaching and denitrification, and extending the fertilizer effect period. Humic acid is a nutrient activator and soil conditioner; its functional groups can chelate nutrients in the biogas slurry, increasing their activity, while also improving soil aggregate structure, enhancing soil water and fertilizer retention capacity, and creating a favorable environment for microorganisms. Micronutrient fertilizers... This product provides essential micronutrients for corn growth, such as iron, zinc, manganese, and copper. The EDTA chelated form ensures high stability in the soil, making it difficult to fix and resulting in extremely high bioavailability, thus compensating for the lack of micronutrients in biogas slurry. The compound microbial agent rapidly decomposes large organic molecules in the biogas slurry, converting them into smaller molecules that are more easily absorbed by plants. Simultaneously, it secretes antibacterial substances to inhibit or kill pathogens that may be present in the biogas slurry, eliminating potential safety hazards. *Bacillus terrestris* colonizes in the soil, continuing to perform nitrogen fixation, phosphorus solubilization, and potassium solubilization, releasing fixed nutrients in the soil and continuously providing nutrition for corn. Plant growth regulators mimic the effects of auxins, directly stimulating root development and stem and leaf growth in corn, regulating the growth cycle, and enhancing stress resistance. In addition to acting as a dispersant, the polymer material itself is a natural biostimulant that promotes plant growth and can also act as a film-forming agent to further aid in the slow release of nutrients.

[0028] Compared with the prior art, the beneficial effects of the present invention are:

[0029] 1. Significantly improve the nutrient utilization efficiency of biogas slurry: Through specific plant growth regulators and optimized synergist formulations and preparation methods, the release of nutrients in biogas slurry can be more balanced and stable, reducing nutrient loss and achieving high-efficiency utilization.

[0030] 2. Synergistic effect of multiple components to achieve multi-effect enhancement: Through the precise ratio and synergistic effect of multiple components such as humic acid, microbial agents, and plant growth regulators, the problem of limited effect of single-component fertilizer enhancers is overcome, and a more comprehensive improvement in fertilizer efficiency is achieved.

[0031] 3. Significantly promotes corn growth and improves quality: This invention significantly improves corn yield and quality by enhancing plant stress resistance and directly stimulating the growth and development of corn roots and above-ground parts. Attached Figure Description

[0032] Figure 1This is the NMR spectrum of the plant growth regulator 1 described in this invention. Detailed Implementation

[0033] The technical solution of the present invention will now be clearly and completely described with reference to the accompanying drawings. Obviously, the described embodiments are merely some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0034] Preparation Example 1

[0035] Preparation of plant growth regulator 1:

[0036]

[0037] Under a nitrogen atmosphere, 15.00 g of raw material A was placed in a double-necked flask, and 200 mL of dichloromethane was added. Then, 35.81 g of 2-(7-azabenzotriazole)-N,N,N',N'-tetramethylurea hexafluorophosphate was added, and the mixture was stirred at room temperature for 1 h. 12.12 g of raw material B was dissolved in 50 mL of dichloromethane and added to the above system. Then, 11.07 g of N,N-diisopropylethylamine was added under an ice-water bath. The ice-water bath was removed, and the system was stirred at room temperature overnight. After the reaction was complete, 100 mL of dichloromethane was added, followed by washing three times with 1M hydrochloric acid aqueous solution, three times with saturated sodium bicarbonate aqueous solution, and the organic phase was washed with saturated brine. The mixture was dried over anhydrous magnesium sulfate, filtered, and evaporated to dryness. Column chromatography was performed, eluting with a mixture of petroleum ether and ethyl acetate, and the eluent was evaporated to dryness to obtain 20.62 g of product 1.

[0038]

[0039] Under nitrogen protection, 20.62 g of product 1 and 22.13 g of raw material C were dissolved in 300 mL of a mixed solvent of 1,4-dioxane / diisopropylamine (200 mL / 100 mL). 0.15 g of palladium on carbon, 1.65 g of 2-dicyclohexylphosphine-2',4',6'-triisopropylbiphenyl, and 0.66 g of CuI were added to the system, and the reaction was carried out at 90 °C for 6 h. After the reaction was completed, the temperature was slightly lowered, and the mixture was filtered through diatomaceous earth to remove salts and catalyst. The reaction solution was concentrated, separated by silica gel column chromatography, using a mixed solvent of n-heptane and ethyl acetate as the eluent, and evaporated to dryness to obtain 30.12 g of plant growth regulator 1.

[0040] Structural assessment:

[0041] Mass spectrometry of Product 1 (M / Z MS+1): 299;

[0042] Mass spectrometry of plant growth regulator 1 (M / Z MS+1:530);

[0043] NMR of plant growth regulator 1: 1 H NMR (Chloroform-d, Figure 1 ): δ9.64-9.54(m,2H),7.94(d,1H),7.67-7.57(m,1H),7.54-7.30(m,4H),7.26-7.07(m,5H),5.86(dd,1H),5.43(d,1H) ,5.32(t,1H),5.18(d,1H),4.45(m,1H),4.14(m,1H),3.96(m,1H),3.85(m,1H),3.75(d,2H),3.59(m,1H),2.34(d,3H).

[0044] Preparation Examples 2-5

[0045] In Preparation Examples 2-5, plant growth regulator 1-plant growth regulator 5 were prepared sequentially, following the preparation method of Preparation Example 1, except that raw material B was replaced, and the rest remained the same as in Preparation Example 1. For details, please refer to Table 1.

[0046] Table 1

[0047]

[0048] Example 1

[0049] Preparation of a nutrient enhancer for maize biogas slurry:

[0050] 1. Raw material components

[0051] Humic acid: 12 parts, selected from: fulvic acid (purchased from: Shanghai Yien Chemical Technology Co., Ltd., CAS: 479-66-3);

[0052] Dicyandiamide: 8 parts (purchased from Shandong Xinheng Chemical Co., Ltd., CAS: 461-58-5);

[0053] Polymer materials: 4 parts, selected from: sodium alginate (purchased from: Shanghai Maclean Biochemical Technology Co., Ltd., CAS: 9005-38-3);

[0054] Micronutrient fertilizer: 1 part, selected from: EDTA chelated iron (CAS: 15708-41-5), EDTA chelated zinc (CAS: 14025-21-9), EDTA chelated manganese (CAS: 15375-84-5), EDTA chelated copper (CAS: 14025-15-1) mixed in a mass ratio of 5:2:2:1;

[0055] Compound microbial agent: 6 parts, selected from: Bacillus amyloliquefaciens (purchased from: Ningbo Mingzhou Biotechnology Co., Ltd.), Bacillus amyloliquefaciens (purchased from: Hubei Qiming Bioengineering Co., Ltd.), and Bacillus subtilis (purchased from: Cangzhou Xingye Biotechnology Co., Ltd.), mixed in a mass ratio of 2:1:1, with each agent containing ≥10 billion CFU / g;

[0056] Suspension agent: 12 parts, selected from: sorbitan fatty acid ester (purchased from: Nantong Haitianyuan Chemical Co., Ltd.);

[0057] Plant growth regulator: 1 part, selected from: plant growth regulator 1 prepared in preparation example 1;

[0058] Diatomaceous earth: 50 portions (purchased from Shandong Zhengxing New Materials Co., Ltd.)

[0059] 2. Preparation method

[0060] S1. Bacillus amyloliquefaciens, Bacillus amyloliquefaciens, and Bacillus subtilis were inoculated into a culture medium containing 22 g / L yeast powder, 20 g / L soybean meal, and 4.5 g / L K2HPO4, respectively. The pH was adjusted to 7.3, and the mixture was fermented in a deep layer at 28°C for 80 hours. After the spore formation rate was greater than 95%, the mixture was mixed with zeolite that had passed through a 60-mesh sieve at a weight ratio of 1:4. The mixture was dried at 35°C and then passed through a 40-mesh sieve to obtain the solid microbial agents. The solid microbial agents were then mixed in proportion to prepare a compound microbial agent.

[0061] S2. Place humic acid, dicyandiamine, polymer material, suspending agent and diatomaceous earth in a stirrer and stir at 300 rpm for 20 minutes to obtain mixture A after thorough mixing;

[0062] S3. Place the micronutrient fertilizer, compound microbial agent and plant growth regulator in a mixer and stir at 300 rpm for 20 minutes until fully mixed to obtain mixture B;

[0063] S4. Add mixture B to mixture A and stir at 300 rpm for 15 minutes at 35°C until evenly dispersed to obtain a nutrient enhancer for corn biogas slurry.

[0064] Examples 2-5

[0065] The preparation of a biogas slurry nutrient enhancer for corn is carried out by referring to the preparation method of Example 1, except that the plant growth regulators are replaced in sequence with plant growth regulators 2-5 prepared in Preparation Examples 2-5, and the rest are the same as in Example 1.

[0066] Comparative Example 1

[0067] The preparation of a nutrient enhancer for maize biogas slurry is carried out according to the preparation method of Example 1, except that no plant growth regulator is added, and the rest is the same as in Example 1.

[0068] Comparative Example 2

[0069] The preparation of a nutrient enhancer for maize biogas slurry is carried out according to the preparation method of Example 1, except that humic acid is not added, and the rest is the same as in Example 1.

[0070] Comparative Example 3

[0071] The preparation of a nutrient enhancer for maize biogas slurry is carried out according to the preparation method of Example 1, except that the compound microbial agent is not added, and the rest is the same as in Example 1.

[0072] Comparative Example 4

[0073] The preparation of a nutrient enhancer for maize biogas slurry is carried out according to the preparation method of Example 1, except that the plant growth regulator is replaced with comparative compound 1, and the rest is the same as in Example 1.

[0074] Comparative compound 1:

[0075] Comparative Example 5

[0076] The preparation of a nutrient enhancer for maize biogas slurry is carried out according to the preparation method of Example 1, except that the plant growth regulator is replaced with indoleacetic acid (CAS: 87-51-4), and the rest is the same as in Example 1.

[0077] Performance testing

[0078] 1. Test materials

[0079] Corn variety: Zhengdan 958;

[0080] Synergist Sample: A nutrient synergist for maize biogas slurry prepared in Examples 1-5 and Comparative Examples 1-5;

[0081] Biogas slurry: Biogas slurry after anaerobic fermentation in large-scale biogas projects (total nitrogen 3.2 g / L, total phosphorus 1.1 g / L, total potassium 2.8 g / L, pH 7.2);

[0082] Experimental site: 0-20cm soil layer basic fertility: organic matter 12.5g / kg, available nitrogen 85mg / kg, available phosphorus 22mg / kg, available potassium 110mg / kg, pH 7.5.

[0083] 2. Experimental Design

[0084] A randomized block design was adopted, with 11 treatments, each with 3 replicates, and a block area of ​​20m². 2;

[0085] Blank control: only biogas slurry was applied;

[0086] Examples 1-5: Biogas slurry + corresponding synergist (synergist: biogas slurry = 1:150, mix and mature for 3 hours before application);

[0087] Comparative Examples 1-5: Biogas slurry + corresponding synergist (synergist: biogas slurry = 1:150, mix and mature for 3 hours before application);

[0088] Fertilization method: 2 irrigations during the jointing stage and the tasseling stage (50% each), and foliar spray once during the tasseling stage (0.3% concentration). Other field management is the same as conventional high-yield cultivation.

[0089] Test metrics

[0090] 1. Vegetative growth indicators: The height of each seedling in each area was measured during the seedling stage, and the average height was calculated. The results are shown in Table 2.

[0091] 2. Yield per hectare: Yield per hectare was measured at maturity and converted to yield per hectare. The results are shown in Table 2.

[0092] 3. Crude protein: The crude protein content was determined by the Kjeldahl method, and the results are shown in Table 2.

[0093] Table 2.

[0094] Example 1 145.3 10650 10.6 Example 2 139.5 10530 10.6 Example 3 141.7 10620 10.4 Example 4 143.2 10640 10.5 Example 5 138.9 10540 10.3 Comparative Example 1 95.8 7240 6.5 Comparative Example 2 105.2 8460 8.1 Comparative Example 3 115.3 8520 8.3 Comparative Example 4 124.6 8830 8.4 Comparative Example 5 115.7 8490 8.3 Blank control 68.3 5380 6.3

[0095] As shown in Table 2, the average plant height, yield per hectare, and crude protein content of maize treated with the synergist containing a specific plant growth regulator and the complete formulation were significantly better than those of the control group and the control group. In the control group, the synergistic effect was relatively weak regardless of whether the plant growth regulator, humic acid, or compound microbial agent was missing, or whether it was replaced with other compounds or conventional indoleacetic acid. This indicates that the synergistic effect of the components in this synergist has a positive impact on maize growth and quality improvement.

[0096] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A nutrient enhancer for corn using biogas slurry, characterized in that, It is composed of the following raw materials in parts by weight: humic acid: 10-15 parts, dicyandiamine: 7-10 parts, polymer materials: 3-5 parts, trace element fertilizer: 0.5-2 parts, compound microbial agent: 5-8 parts, suspension agent: 10-15 parts, plant growth regulator: 0.5-2 parts, diatomaceous earth: 40-65 parts; The plant growth regulator is a compound represented by Formula 1: Formula 1: ; R1 in Formula 1 is a substituent; R1 is methyl, ethyl, methoxy, nitro, or phenyl. The polymer material is sodium alginate; The compound microbial agent is composed of Bacillus amyloliquefaciens, Bacillus amyloliquefaciens and Bacillus subtilis in a mass ratio of 2:1:1, and the bacterial content of each agent is ≥10 billion CFU / g. The suspending agent is sorbitol fatty acid ester.

2. The nutrient enhancer for maize biogas slurry as described in claim 1, characterized in that, The humic acid is at least one of fulvic acid or nitrohumic acid.

3. The nutrient enhancer for maize biogas slurry according to claim 1, characterized in that, The micronutrient fertilizer is composed of EDTA chelated iron, EDTA chelated zinc, EDTA chelated manganese, and EDTA chelated copper in a mass ratio of 5:2:2:

1.

4. The nutrient enhancer for corn using biogas slurry according to claim 1, characterized in that, The plant growth regulator is any one of the compounds shown in the following structures: ； ； 。 5. A method for preparing a nutrient enhancer for corn biogas slurry according to any one of claims 1-4, characterized in that, Includes the following steps: S1. The Bacillus amyloliquefaciens, Bacillus amyloliquefaciens and Bacillus subtilis are subjected to liquid deep fermentation. After the spore formation rate is greater than 95%, they are mixed with zeolite that has passed through a 60-mesh sieve at a weight ratio of 1:

4. After drying at 30°C, they are passed through a 40-mesh sieve to obtain solid microbial agents. They are then mixed in proportion to prepare a compound microbial agent. S2. Place the humic acid, dicyandiamine, polymer material, suspending agent and diatomaceous earth in a stirrer and stir at 200-400 rpm for 15-30 minutes to make them fully mixed to obtain mixture A; S3. Place the micronutrient fertilizer, compound microbial agent and plant growth regulator in a mixer and stir at 200-400 rpm for 15-30 minutes to ensure thorough mixing and obtain mixture B; S4. Add the mixture B to the mixture A, stir and mix at 25-45℃ for 10-20 minutes to disperse evenly and obtain a corn biogas slurry nutrient enhancer.

6. The method for preparing a nutrient enhancer for maize biogas slurry according to claim 5, characterized in that, The culture medium for deep fermentation in S1 consists of 20-24 g / L yeast powder, 18-24 g / L soybean meal, and 0.8-8.5 g / L K2HPO4, with the pH adjusted to 7.0-7.6, the culture temperature at 28±2℃, and the culture time at 72-96 hours.

7. A method of using a nutrient enhancer for corn slurry as described in any one of claims 1-4, characterized in that, Mix the synergist with biogas slurry at a mass ratio of 1:(100-200), stir evenly, and let stand for 2-4 hours to mature. Then it can be irrigated with water or sprayed on the leaves.

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