A prostate small body excretion protein detection box for early screening of prostate diseases

Abstract

The application provides a prostate body extracellular protein detection box for early screening of prostate diseases, relates to the technical field of detection kits, and comprises a base, a moving frame threadedly installed at the left end of the base, an electric telescopic rod and an optical probe fixedly installed at the outer end of the moving frame, an electromagnetic magnetic head fixedly installed at the bottom of the electric telescopic rod, and a group of protective covers slidingly installed at the right end of the base. The quick disassembly, assembly and accurate positioning of the detection disc are realized through cooperation of a limiting block and an elastic element, and the detection disc can be quickly rotated to switch the function partition. The moving frame is driven by a motor to link the electromagnetic magnetic head and the optical probe to complete the whole process of sample adsorption, cleaning and detection. The protective cover is slidingly designed through the elastic element to realize quick opening and closing, and effectively improves the detection protection performance. The problems of complex detection disc mounting structure, complicated disassembly and assembly process, influence on detection precision and equipment service life, and lack of effective partition isolation and automatic control mechanism in the sample processing process during detection are solved.

Description

Technical Field

[0001] This invention relates to the field of test kit technology, and in particular to a prostate small effusive protein detection kit for early screening of prostate diseases. Background Technology

[0002] Prostate diseases are a major category of diseases affecting men in my country, especially with the increasing incidence of prostatitis. Statistics show that the etiology of chronic prostatitis is very complex. Currently, it is believed that prostatitis may be a primary or secondary disease of the prostate and its surrounding tissues, organs, muscles, and nerves, often resulting from the combined effects of multiple factors through different mechanisms. Even after these diseases have been cured or completely eradicated, the damage and pathological changes they caused continue to exert their effects independently. The core cause may be the combined effect of infection, inflammation, and abnormal pelvic floor neuromuscular activity. Due to the complex and diverse etiologies and pathological changes of prostatitis, and its impact on male sexual and reproductive functions, prostatitis severely affects patients' quality of life, causing them great mental and physical suffering. Therefore, early diagnosis and treatment are crucial. Thus, there is a need to develop a prostate small effusive protein detection kit for early screening of prostate diseases.

[0003] Currently available prostate small effusive protein test kits for early screening of prostate diseases have complex test tray installation structures and cumbersome disassembly and assembly processes, which increase the difficulty and time cost of maintenance and cleaning. They are also prone to damage to the test tray during operation, affecting the detection accuracy and equipment lifespan. Furthermore, the sample processing flow during the detection process lacks effective zoning isolation and automated control mechanisms. Summary of the Invention

[0004] This invention relates to a prostate small effusive protein detection kit for early screening of prostate diseases. It utilizes a limiting block and elastic element to achieve rapid assembly and disassembly of the detection disc and precise positioning. A drive motor rotates the detection disc to switch functional zones. A moving frame, driven by a motor, links the electromagnetic head and optical probe to complete the entire process of sample adsorption, cleaning, and detection, avoiding cross-contamination. The protective cover features a sliding design with elastic elements for rapid opening and closing, effectively improving detection and protection performance.

[0005] In a first aspect, the present invention provides a small prostatic effusive protein detection kit for early screening of prostate diseases, specifically comprising: a base; A set of movable frames is threaded onto the left end of the base. A set of electric telescopic rods and a set of optical probes are fixedly installed on the outer end of the movable frames. A set of electromagnetic heads is fixedly installed on the bottom of the electric telescopic rods. A set of protective covers is slidably installed on the right end of the base. A set of handles is fixedly installed on the outer end of the protective covers. A set of detection discs is also installed on the top of the base. A set of limiting ring grooves is opened at the bottom of the detection discs. A set of limiting blocks is also slidably installed on the base. The limiting blocks on the base are inserted into the limiting ring grooves at the bottom of the detection discs. The detection discs are transparent circular discs. The detection discs have a sample chamber, a lysis chamber, an immunoreaction chamber, a washing chamber, and an optical detection chamber integrated in a circular array. A flow-guiding pump is also installed in the detection discs. An electromagnet array is embedded at the bottom of the immunoreaction chambers. The optical detection chambers are transparent chambers. The optical detection chambers are pre-filled with lyophilized fluorescent marker bulbs and a hollow calibration area for encapsulating standards of known concentrations.

[0006] Furthermore, a set of first elastic elements is also sleeved on the connecting rod at the outer end of the limiting block, and the first elastic elements are in contact with the inner wall of the sliding hole on the base.

[0007] Furthermore, a flow-guiding pump is installed between the sample chamber and the lysis chamber, the lysis chamber and the immune reaction chamber, and the washing chamber and the optical detection chamber.

[0008] Furthermore, a set of guide rods is fixedly installed at the rear end of the base, and a set of connecting holes are opened at the rear end of the movable frame, and the connecting holes at the rear end of the movable frame are slidably installed on the guide rods at the rear end of the base.

[0009] Furthermore, a set of sliding holes is provided at the bottom outer end of the base, and a set of connecting rods is fixedly installed at the outer end of the limiting block, and the connecting rods at the outer end of the limiting block are slidably installed on the sliding holes at the bottom outer end of the base.

[0010] Furthermore, a set of connecting plates is fixedly installed at the front and rear ends of the base, and sliding holes are provided on the connecting plates. A set of fixing rods is fixedly installed at the front and rear ends of the protective cover, and a second elastic element is sleeved on the fixing rods. The fixing rods at the front and rear ends of the protective cover are slidably installed on the sliding holes of the connecting plates at the front and rear ends of the base.

[0011] Furthermore, a set of heat-conducting plates is fixedly installed at the bottom of the pyrolysis chamber on the detection plate, and a set of heating plates is also fixedly installed at the corresponding position on the side end of the spline shaft.

[0012] Furthermore, the optical probe consists of an LED light source, a filter, and a photoelectric sensor, and the optical probe can move linearly along the radius of the detection disk.

[0013] Furthermore, a set of adjusting screws is rotatably mounted on the front end of the base, a set of motors is fixedly mounted on the side end of the base, the outer end of the adjusting screws is fixedly mounted on the drive shaft of the motors, and a set of threaded holes is opened at the front end of the movable frame, and the threaded holes at the front end of the movable frame are threaded onto the adjusting screws at the front end of the base.

[0014] Furthermore, a set of drive motors is fixedly installed at the bottom of the base, a splined shaft is fixedly installed on the drive motor's transmission shaft, a positioning groove is opened at the bottom of the detection plate, and the positioning groove at the bottom of the detection plate is snapped onto the splined shaft of the drive motor's transmission shaft.

[0015] This invention provides a prostate small follicle protein detection kit for early screening of prostate diseases, which has the following beneficial effects: In this invention, a limiting block, connected by a connecting rod and an elastic element, enables rapid engagement between the detection disk and the splined shaft of the drive motor, as well as precise positioning of the limiting ring groove, facilitating disassembly and maintenance. Simultaneously, the drive motor rotates the detection disk, allowing each functional zone to move to its desired working position. The moving frame, through the coordinated action of threaded holes, adjusting screws, and guide rods, enables the left-right movement of the electromagnetic head and optical probe under motor drive. Combined with an electric telescopic rod controlling the lifting and lowering of the magnetic head, this sequentially completes the adsorption of the magnetic bead-PSEP complex in the immunoreaction chamber, the release and cleaning of the complex in the cleaning chamber, and the final scanning detection in the optical detection chamber, effectively preventing sample cross-contamination. The protective cover, slidably mounted on the base via an elastic element, can be quickly opened and closed to cover the detection disk, significantly improving dust protection during the detection process.

[0016] Furthermore, the connecting rod at the outer end of the limiting block is slidably installed on the sliding hole at the outer end of the bottom of the base. Pulling the limiting block outward will engage the positioning groove at the bottom of the detection plate with the spline shaft of the drive motor. After the detection plate moves to a fixed position, the limiting block is released. Under the action of the first elastic element on the connecting rod, the limiting block on the base is inserted into the limiting ring groove at the bottom of the detection plate. This makes the detection plate of this device easy to disassemble and assemble quickly, improving the maintenance and cleaning efficiency of the device. It can also drive the detection plate to rotate through the drive motor, so that the different sections on the detection plate move sequentially to the working position on the left end.

[0017] Furthermore, the movable frame is threaded onto the adjusting screw at the front of the base via a threaded hole at the front end, and slidably mounted onto the guide rod at the rear end of the base via a connecting hole at the rear end. A motor drives the adjusting screw to rotate, causing the electromagnetic head and optical probe to move left and right. The optical probe can scan the optical detection chamber. An electric telescopic rod moves the electromagnetic head up and down, lowering it above the immunoreaction chamber, where it adsorbs the magnetic bead-PSEP complex. The detection disc is then rotated until the cleaning chamber is below the electromagnetic head, at which point the power is cut off to release the complex. This adsorption / release action is repeated to complete the cleaning process. The detection disc is then rotated again until the optical detection chamber is below the electromagnetic head. After cleaning, the adsorbed complex from the electromagnetic head is released into the optical detection chamber for scanning, thus preventing sample contamination and reducing operational risks.

[0018] In addition, the fixing rods at the front and rear ends of the protective cover are slidably installed on the sliding holes of the connecting plates at the front and rear ends of the base, respectively. With the second elastic element on the fixing rod, the device can easily pass through the protective cover to detect and protect the detection plate, which effectively improves the detection and protection effect of the device. Attached Figure Description

[0019] To more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings of the embodiments will be briefly described below.

[0020] The accompanying drawings described below are only related to some embodiments of the invention and are not intended to limit the invention.

[0021] In the attached diagram: Figure 1 This is a front-side axial schematic diagram of the prostate small effusion protein detection kit for early screening of prostate diseases according to the present invention.

[0022] Figure 2 This is a rear-axis schematic diagram of the prostate small effusion protein detection kit for early screening of prostate diseases according to the present invention.

[0023] Figure 3 This is a schematic diagram showing the disassembly and assembly of the protective cover of the prostate small effusive protein detection kit for early screening of prostate diseases according to the present invention.

[0024] Figure 4 This is a schematic diagram of the top of the detection tray of the prostate small effusive protein detection kit for early screening of prostate diseases according to the present invention.

[0025] Figure 5 This is a schematic diagram illustrating the disassembly and assembly of the movable frame of the prostate small effusion protein detection kit for early screening of prostate diseases according to the present invention.

[0026] Figure 6This is a schematic diagram showing the assembly and disassembly of the detection tray of the prostate small effusive protein detection kit for early screening of prostate diseases according to the present invention.

[0027] Figure 7 This is a schematic diagram of the bottom of the detection tray of the prostate small effusive protein detection kit for early screening of prostate diseases according to the present invention.

[0028] Figure 8 This is a schematic diagram of the installation of the detection tray of the prostate small effusive protein detection kit for early screening of prostate diseases according to the present invention.

[0029] Figure 9 This is a schematic diagram of the installation of the optical probe of the prostate small effusive protein detection kit for early screening of prostate diseases according to the present invention.

[0030] List of reference numerals 1. Base; 101. Connecting plate; 102. Guide rod; 103. Adjusting screw; 104. Drive motor; 1041. Splined shaft; 105. Heating plate; 106. Limiting block; 1061. Connecting rod; 1062. First elastic element; 2. Protective cover; 201. Handle; 202. Fixing rod; 203. Second elastic element; 3. Moving frame; 301. Connecting hole; 302. Threaded hole; 303. Electric telescopic rod; 3031. Electromagnetic head; 304. Optical probe; 4. Detection plate; 401. Sample chamber; 402. Lysis chamber; 403. Immunoreaction chamber; 404. Cleaning chamber; 405. Optical detection chamber; 406. Limiting ring groove; 407. Positioning groove; 408. Heat-conducting sheet; 5. Flow guiding connection pump. Detailed Implementation

[0031] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of the present invention. Based on the described embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0032] Please refer to Figures 1 to 9 As shown: Example 1: This invention provides a prostate small effusion protein detection kit for early screening of prostate diseases, including a base 1; A set of movable frames 3 are threadedly installed on the left end of the base 1. A set of electric telescopic rods 303 and a set of optical probes 304 are fixedly installed on the outer end of the movable frames 3. A set of electromagnetic heads 3031 are fixedly installed on the bottom of the electric telescopic rods 303. A set of protective covers 2 are slidably installed on the right end of the base 1. A set of handles 201 are fixedly installed on the outer end of the protective covers 2. A set of detection discs 4 are also installed on the top of the base 1. A set of limiting ring grooves 406 are opened at the bottom of the detection discs 4. A set of limiting blocks 106 are also slidably installed on the base 1. Block 106 is inserted and installed on the limiting ring groove 406 at the bottom of the detection disk 4. The detection disk 4 is a transparent circular disk. The detection disk 4 has a sample chamber 401, a lysis chamber 402, an immunoreaction chamber 403, a washing chamber 404, and an optical detection chamber 405 integrated in a circular array. The detection disk 4 is also equipped with a flow guide pump 5. The bottom of the immunoreaction chamber 403 is embedded with an electromagnet array. The optical detection chamber 405 is a transparent cavity. The optical detection chamber 405 is pre-filled with lyophilized fluorescent marker bulbs and a hollow calibration area for encapsulating standards of known concentrations.

[0033] In this embodiment of the invention, as follows: Figures 6 to 8 As shown, a set of drive motors 104 are fixedly installed at the bottom of the base 1. A splined shaft 1041 is fixedly installed on the drive shaft of the drive motors 104. A positioning groove 407 is opened at the bottom of the detection disk 4, and the positioning groove 407 at the bottom of the detection disk 4 is snapped onto the splined shaft 1041 of the drive shaft of the drive motor 104. A set of sliding holes is opened at the outer end of the bottom of the base 1. A set of connecting rods 1061 is fixedly installed at the outer end of the limiting block 106, and the connecting rods 1061 at the outer end of the limiting block 106 are slidably installed on the sliding holes at the outer end of the bottom of the base 1. A set of first elastic members 1062 are also sleeved on the connecting rods 1061 at the outer end of the limiting block 106, and the first elastic members 1062 are in contact with the inner wall of the sliding holes on the base 1. Specifically, The connecting rod 1061 at the outer end of the limiting block 106 is slidably installed on the sliding hole at the outer end of the bottom of the base 1. Pulling the limiting block 106 outward will engage the positioning groove 407 at the bottom of the detection disk 4 with the splined shaft 1041 of the drive motor 104. After the detection disk 4 moves to the fixed position, the limiting block 106 is released. Under the action of the first elastic element 1062 on the connecting rod 1061, the limiting block 106 on the base 1 is inserted into the limiting ring groove 406 at the bottom of the detection disk 4. This makes it easy and quick to disassemble and assemble the detection disk 4 of this device, improves the maintenance and cleaning efficiency of the device, and can drive the detection disk 4 to rotate through the drive motor 104, so that the different sections on the detection disk 4 move sequentially to the working position on the left end.

[0034] Among them, such as Figures 5 to 7As shown, a set of heat-conducting plates 408 are fixedly installed at the bottom of the lysis chamber 402 on the detection disk 4. A set of heating plates 105 are also fixedly installed at the corresponding position on the side end of the spline shaft 1041. A flow-guiding pump 5 is installed between the sample chamber 401 and the lysis chamber 402, the lysis chamber 402 and the immune reaction chamber 403, and the washing chamber 404 and the optical detection chamber 405. The optical probe 304 consists of an LED light source, a filter, and a photoelectric sensor, and the optical probe 304 can move linearly along the radial direction of the detection disk 4. A set of guide rods 102 is fixedly installed at the rear end of the base 1. A set of connecting holes 301 is opened at the rear end of the movable frame 3, and the connecting holes 301 at the rear end of the movable frame 3 are slidably installed on the guide rods 102 at the rear end of the base 1. A set of adjusting screws 103 is rotatably installed at the front end of the base 1. A set of motors is fixedly installed at the side end of the base 1. The outer end of the adjusting screws 103 is fixedly installed on the drive shaft of the motor. A set of threaded holes 302 is opened at the front end of the movable frame 3, and the threaded holes 302 at the front end of the movable frame 3 are threadedly installed on the base. The adjustment screw 103 at the front end of the base 1 is threaded onto the adjustment screw 103 at the front end of the base 1 via the threaded hole 302 at the front end of the moving frame 3. The connecting hole 301 at the rear end of the moving frame 3 is slidably mounted onto the guide rod 102 at the rear end of the base 1. The motor drives the adjustment screw 103 to rotate, thereby moving the electromagnetic head 3031 and the optical probe 304 left and right. The optical probe 304 can scan and detect inside the optical detection chamber 405. The electric telescopic rod 303 drives the electromagnetic head 3031 to rise and fall, descending to above the immunoreaction chamber 403, where it is energized to adsorb the magnetic bead-PSEP complex. The detection disk 4 is rotated to the cleaning chamber 404 below the electromagnetic head 3031. The complex is released when the power is turned off. The adsorption / release action is repeated to complete the cleaning. The detection disk 4 is rotated again to the optical detection chamber 405 below the electromagnetic head 3031. After cleaning, the adsorbed complex of the electromagnetic head 3031 is released into the optical detection chamber 405 for scanning and detection, thereby preventing sample contamination and reducing operational risks.

[0035] Example 2: Based on Example 1, wherein, as Figure 3 As shown, a set of connecting plates 101 are fixedly installed at the front and rear ends of the base 1, and sliding holes are provided on the connecting plates 101. A set of fixing rods 202 are fixedly installed at the front and rear ends of the protective cover 2, and a second elastic element 203 is sleeved on the fixing rods 202. The fixing rods 202 at the front and rear ends of the protective cover 2 are slidably installed on the sliding holes of the connecting plates 101 at the front and rear ends of the base 1. Specifically, the fixing rods 202 at the front and rear ends of the protective cover 2 are slidably installed on the sliding holes of the connecting plates 101 at the front and rear ends of the base 1, and cooperate with the second elastic element 203 on the fixing rods 202 to make it easier for the device to detect and protect the detection plate 4 through the protective cover 2, thereby effectively improving the detection and protection effect of the device.

[0036] The specific usage and function of this embodiment: In this invention, the connecting rod 1061 at the outer end of the limiting block 106 is slidably installed on the sliding hole at the outer end of the bottom of the base 1. Pulling the limiting block 106 outwards will engage the positioning groove 407 at the bottom of the detection disk 4 with the spline shaft 1041 of the drive motor 104. After the detection disk 4 moves to a fixed position, the limiting block 106 is released. Under the action of the first elastic element 1062 on the connecting rod 1061, the limiting block 106 on the base 1 is inserted and installed on the bottom of the detection disk 4. The limiting ring groove 406 of the part allows the detection plate 4 of this device to be easily and quickly disassembled and assembled, improving the maintenance and cleaning efficiency of the device. It can also be driven by the drive motor 104 to rotate the detection plate 4, causing different sections on the detection plate 4 to move sequentially to the working position on the left end. The threaded hole 302 at the front end of the moving frame 3 is threaded onto the adjusting screw 103 at the front end of the base 1, and the connecting hole 301 at the rear end of the moving frame 3 is slidably mounted onto the guide rod 102 at the rear end of the base 1. The motor drives the adjusting screw 103 to rotate, so that... The electromagnetic head 3031 and optical probe 304 are moved left and right. The optical probe 304 can scan and detect inside the optical detection chamber 405. The electric telescopic rod 303 drives the electromagnetic head 3031 to rise and fall, descending to above the immunoreaction chamber 403, where it is energized to adsorb the magnetic bead-PSEP complex. The detection disk 4 is rotated until the cleaning chamber 404 is below the electromagnetic head 3031. The complex is released when the power is turned off. The adsorption / release action is repeated to complete the cleaning. The detection disk 4 is rotated again until the optical detection chamber 405 is below the electromagnetic head 3031. After cleaning, the adsorbed complex of the electromagnetic head 3031 is released into the optical detection chamber 405 for scanning and detection, thereby preventing sample contamination and reducing operational risks. The fixing rods 202 at the front and rear ends of the protective cover 2 are slidably installed on the sliding holes of the connecting plates 101 at the front and rear ends of the base 1. With the help of the second elastic element 203 on the fixing rod 202, the device can easily detect and protect the detection disk 4 through the protective cover 2, effectively improving the detection and protection effect of the device.

Claims

1. A prostate small effusion protein detection kit for early screening of prostate diseases, characterized in that, Including the base (1); A set of movable frames (3) is threaded onto the left end of the base (1). A set of electric telescopic rods (303) and a set of optical probes (304) are fixedly installed on the outer end of the movable frames (3). A set of electromagnetic heads (3031) is fixedly installed on the bottom of the electric telescopic rods (303). A set of protective covers (2) is slidably installed on the right end of the base (1). A set of handles (201) is fixedly installed on the outer end of the protective covers (2). A set of detection discs (4) is also installed on the top of the base (1). A set of limiting ring grooves (406) is opened at the bottom of the detection discs (4). A set of limiting blocks (106) is also slidably installed on the base (1). The limiting blocks (106) on the base (1) are inserted into the detection discs. On the limiting ring groove (406) at the bottom of the test plate (4), the test plate (4) is a transparent circular plate. The test plate (4) has a sample chamber (401), a lysis chamber (402), an immunoreaction chamber (403), a washing chamber (404), and an optical detection chamber (405) arranged in a circular array. The test plate (4) is also equipped with a flow-guiding pump (5). The bottom of the immunoreaction chamber (403) is embedded with an electromagnet array, and the optical detection chamber (405) is a transparent cavity. The optical detection chamber (405) is pre-filled with lyophilized fluorescent marker spheres and a hollow calibration area for encapsulating known concentration standards. A set of drive motors (104) is fixedly installed at the bottom of the base (1). A splined shaft (1041) is fixedly installed on the drive shaft of the 04), and a positioning groove (407) is provided at the bottom of the detection disk (4), and the positioning groove (407) at the bottom of the detection disk (4) is snapped onto the splined shaft (1041) of the drive shaft of the drive motor (104); a set of sliding holes is provided at the bottom outer end of the base (1), and a set of connecting rods (1061) is fixedly installed at the outer end of the limiting block (106), and the connecting rods (1061) at the outer end of the limiting block (106) are slidably installed on the sliding holes at the bottom outer end of the base (1); a set of first elastic elements (1062) is also sleeved on the connecting rods (1061) at the outer end of the limiting block (106), and the first elastic elements (1062) are sleeved onto the connecting rods (1061) at the outer end of the limiting block (106). 62) It is in contact with the inner wall of the sliding hole on the base (1); a set of heat-conducting plates (408) are fixedly installed at the bottom of the cracking chamber (402) on the detection plate (4), and a set of heating plates (105) are fixedly installed at the corresponding position on the side end of the spline shaft (1041); a set of connecting plates (101) are fixedly installed at the front and rear ends of the base (1), and sliding holes are opened on the connecting plates (101). A set of fixing rods (202) are fixedly installed at the front and rear ends of the protective cover (2), and a second elastic element (203) is sleeved on the fixing rods (202). The fixing rods (202) at the front and rear ends of the protective cover (2) are slidably installed on the sliding holes of the connecting plates (101) at the front and rear ends of the base (1).

2. The prostate small effusive protein detection kit for early screening of prostate diseases according to claim 1, characterized in that: A flow-guiding pump (5) is installed between the sample chamber (401) and the lysis chamber (402), the lysis chamber (402) and the immune reaction chamber (403), and the washing chamber (404) and the optical detection chamber (405).

3. The prostate small effusive protein detection kit for early screening of prostate diseases according to claim 1, characterized in that: The optical probe (304) consists of an LED light source, a filter and a photoelectric sensor, and the optical probe (304) can move linearly along the radial direction of the detection disk (4).

4. The prostate small effusive protein detection kit for early screening of prostate diseases according to claim 1, characterized in that: A set of guide rods (102) is fixedly installed at the rear end of the base (1), and a set of connecting holes (301) is opened at the rear end of the movable frame (3), and the connecting holes (301) at the rear end of the movable frame (3) are slidably installed on the guide rods (102) at the rear end of the base (1).

5. A prostate small effusive protein detection kit for early screening of prostate diseases according to claim 1, characterized in that: A set of adjusting screws (103) is rotatably installed at the front end of the base (1), and a set of motors is fixedly installed at the side end of the base (1). The outer end of the adjusting screws (103) is fixedly installed on the drive shaft of the motor. A set of threaded holes (302) is opened at the front end of the moving frame (3), and the threaded holes (302) at the front end of the moving frame (3) are threaded onto the adjusting screws (103) at the front end of the base (1).

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