Kit for detecting ApoE genotyping and application thereof

By using MSD technology to detect the ratio of ApoE4 protein to ApoE protein, the complexity and sensitivity issues of existing ApoE genotyping detection methods are resolved, enabling rapid and accurate genotyping analysis applicable to a variety of biological samples.

CN121049518APending Publication Date: 2025-12-02UNIV OF SCI & TECH OF CHINA
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Patent Information

Application Number
CN202410682455.X
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-05-29
Publication Date
2025-12-02

AI Technical Summary

Technical Problem

Existing ApoE genotyping methods suffer from problems such as complex operation, low sensitivity, narrow sample applicability, and high risk of misoperation, making it difficult to achieve rapid and accurate genotyping analysis, especially in large-scale sample testing.

Method used

Using MSD (Meso Scale Discovery) technology, the ratio of ApoE4 protein concentration to ApoE protein concentration in the serum sample of the subject is detected, and combined with specific buffer and antibody, to achieve rapid detection of ApoE genotyping.

Benefits of technology

It improves the sensitivity and accuracy of detection, simplifies the operation process, reduces the sample volume requirement, is applicable to a variety of biological samples, reduces matrix effects, and achieves efficient genotyping analysis.

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Abstract

The invention provides a kit for detecting ApoE (Apolipoprotein E) genotyping of a subject by utilizing an MSD (Meso Scale Discovery) technology and an application of the kit. The kit provided by the invention has accurate measurement and specific recognition on the content detection of human serum sample ApoE4, and compared with the traditional ELISA, the kit has the advantages of higher sensitivity, wider linear range, high sample compatibility and small matrix effect.
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Citation Information

Patent Citations

  • ApoE gene typing detection kit suitable for blood genome DNA sample and PCR method therefor

    CN109536597A

  • A human ApoE genotyping kit

    CN111269978B