A composition containing mussel extract, a preparation method and applications thereof

Abstract

The application relates to a composition containing mussel extract and application thereof, which is composed of mussel extract, extract of Alnus nepalensis D.Don, extract of Eucommia ulmoides male flowers, triethanolamine, a thickening agent, a preservative and deionized water. The efficient synergistic system formed by the mussel extract, the extract of Alnus nepalensis D.Don and the extract of Eucommia ulmoides male flowers not only has the comprehensive effects of efficient superposition whitening, moisturizing and wrinkle removal, but also greatly reduces the risk of allergy due to the natural and mild characteristics of the components. Specifically, the mussel extract forms a water-locking barrier on the skin surface to create a humid base for the penetration and absorption of subsequent active ingredients; the collagen synthesis promoting effect of the mussel extract is complementary to the antioxidant capacity of the active ingredients in the extract of Alnus nepalensis D.Don and the extract of Eucommia ulmoides male flowers, which can not only eliminate free radicals to reduce collagen damage, but also actively promote collagen regeneration to fade lines, and can also synergistically exert the whitening efficiency of inhibiting melanin activity and reducing pigment deposition, and can balance the moisturizing texture to avoid stickiness or dryness and other discomforts.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, and in particular to a composition containing mussel extract, its preparation method, and its application. Background Technology

[0002] In today's cosmetics market, consumers' pursuit of skincare product efficacy is becoming increasingly diversified, with whitening, moisturizing, and anti-wrinkle becoming core demands that have garnered significant attention. Regarding whitening, excessive melanin production is a key factor leading to dull skin and the formation of age spots. Most existing whitening products only act on a single aspect (such as inhibiting tyrosinase activity). This single-action approach makes it difficult to comprehensively regulate the entire process of melanin production, transport, and metabolism, resulting in unsatisfactory whitening effects and a tendency for rebound. In the area of ​​moisturizing, skin hydration primarily relies on natural moisturizing factors in the stratum corneum and the integrity of the skin barrier. Common moisturizers such as glycerin and hyaluronic acid can temporarily increase skin moisture content, but their moisturizing effect is poor and cannot fundamentally improve the skin barrier function. In the realm of anti-wrinkle, with age, collagen and elastin fibers in the skin gradually decrease and break down, leading to sagging skin and wrinkles.

[0003] Mussel extract, as an emerging natural active ingredient, is gradually gaining attention in cosmetic research and development. Mussel extract contains various bioactive substances, such as mussel adhesive protein, polysaccharides, amino acids, peptides, and minerals, showing potential in repair and whitening. However, current research on scientifically combining mussel extract with other cosmetic ingredients to achieve synergistic effects of whitening, moisturizing, and anti-wrinkle is limited, making it difficult to meet consumers' demand for comprehensive and highly effective skincare products.

[0004] Therefore, in view of the shortcomings of the existing technology, it is necessary to provide a composition containing mussel extract, a preparation method and its application to solve the shortcomings of the existing technology. Summary of the Invention

[0005] The primary objective of this invention is to overcome the shortcomings of existing technologies by providing a composition containing mussel extract. This composition containing mussel extract exhibits superior whitening, moisturizing, and wrinkle-reducing effects.

[0006] The above-mentioned objectives of the present invention are achieved through the following technical measures:

[0007] A composition containing mussel extract is provided, comprising mussel extract, septum extract, Eucommia ulmoides male flower extract, triethanolamine, thickener, preservative and deionized water.

[0008] In the composition containing mussel extract, by weight,

[0009] Mussel extract: 1.50 parts to 1.80 parts;

[0010] Extract of septum husk: 0.20 to 0.50 parts;

[0011] Eucommia ulmoides male flower extract: 0.50 parts to 0.80 parts;

[0012] Triethanolamine: 0.50 parts to 0.80 parts;

[0013] Thickener: 0.05 parts to 0.10 parts;

[0014] Preservative: 0.10 parts to 0.50 parts;

[0015] Add deionized water to bring the total to 100 parts.

[0016] The preparation method of the septum extract is as follows: the septum is extracted with ethanol aqueous solution, the extract is concentrated and then extracted with ethyl acetate, the organic phase is separated, and the organic phase is concentrated to obtain the septum extract.

[0017] The preparation method of the Eucommia ulmoides male flower extract is as follows: dried Eucommia ulmoides male flowers are extracted with petroleum ether, the extract is concentrated and then extracted with an aqueous ethanol solution, and the extract is concentrated to obtain the Eucommia ulmoides male flower extract.

[0018] In the composition containing mussel extract, by weight,

[0019] Mussel extract: 1.68 parts;

[0020] Quartz extract: 0.35 parts;

[0021] Eucommia ulmoides male flower extract: 0.65 parts;

[0022] Triethanolamine: 0.60 parts;

[0023] Thickener: 0.07 parts;

[0024] Preservative: 0.30 parts;

[0025] Add deionized water to bring the total to 100 parts.

[0026] Preferably, the preparation method of the above-mentioned septum extract is as follows:

[0027] A1. Crush the septum wood, add an ethanol aqueous solution and heat to extract, filter to obtain the extract, and concentrate the extract to obtain septum wood concentrate.

[0028] A2. The concentrated extract of septum obtained in A1 was added to ethyl acetate and stirred. The organic phase was then separated by standing. The organic phase was then concentrated and dried under vacuum to obtain the septum extract.

[0029] Preferably, the preparation method of the above-mentioned septum extract is as follows:

[0030] A1. Crush the septum into 20-30 mesh, add 10-16 times the weight of an ethanol aqueous solution with a mass percentage concentration of 30%-50%, and extract at 60-80℃ for 3-5 hours. Filter to obtain the extract, and concentrate the extract to 1 / 3-1 / 4 of its volume to obtain the septum concentrate.

[0031] A2. Add 4 to 8 times the weight of the concentrated septum obtained in A1 to ethyl acetate and stir for 0.5 to 2 hours. Then, allow the mixture to stand to separate the organic phase. Concentrate and dry the organic phase under vacuum at 40 to 50 degrees Celsius to obtain the septum extract.

[0032] Preferably, the preparation method of the above-mentioned septum extract is as follows:

[0033] A1. Crush the septum into 25 mesh, add 15 times its weight of ethanol aqueous solution with a mass percentage concentration of 45%, extract at 70℃ for 4 hours, filter and collect the extract, and concentrate the extract to 1 / 4 volume to obtain septum concentrate.

[0034] A2. Add 6 times the weight of the concentrated septum obtained in A1 to ethyl acetate and stir for 1 hour. After standing, separate the organic phase and concentrate and dry the organic phase under vacuum at 45°C to obtain the septum extract.

[0035] Preferably, the preparation method of the above-mentioned Eucommia ulmoides male flower extract is as follows:

[0036] B1. Crush the dried male flowers of Eucommia ulmoides, add petroleum ether, heat and stir to extract, and filter to collect the residue.

[0037] B2. Add the filter residue obtained in B1 to an ethanol-water solution for extraction, separate the extract, and concentrate and dry the extract under vacuum to obtain Eucommia ulmoides male flower extract.

[0038] Preferably, the preparation method of the above-mentioned Eucommia ulmoides male flower extract is as follows:

[0039] B1. Pulverize the dried male flowers of Eucommia ulmoides to 50-80 mesh, add 10-15 times the weight of petroleum ether after pulverization, extract at 40-50℃ for 1-2 hours, filter and take the residue.

[0040] B2. Add the filter residue obtained in B1 to an ethanol aqueous solution with a mass percentage concentration of 40% to 60% and a weight of 10 to 15 times to extract the extract. Separate the extract and concentrate and dry the extract under vacuum to obtain Eucommia ulmoides male flower extract.

[0041] Preferably, the preparation method of the above-mentioned Eucommia ulmoides male flower extract is as follows:

[0042] B1. Pulverize the dried male flowers of Eucommia ulmoides to 60 mesh, add 13 times the weight of petroleum ether after pulverization, extract at 45℃ for 1.5h, filter and collect the residue.

[0043] B2. Add the filter residue obtained in B1 to an ethanol aqueous solution with a mass percentage concentration of 50% (12 times its weight) for extraction, separate the extract, and concentrate and dry the extract under vacuum to obtain Eucommia ulmoides male flower extract.

[0044] The second objective of this invention is to overcome the shortcomings of the prior art by providing a method for preparing a composition containing mussel extract. The composition containing mussel extract obtained by this method has superior whitening, moisturizing, and wrinkle-reducing effects.

[0045] The above-mentioned objectives of the present invention are achieved through the following technical measures:

[0046] A method for preparing the above-mentioned composition containing mussel extract is provided, wherein mussel extract, septum extract, Eucommia ulmoides male flower extract, triethanolamine, thickener, preservative and deionized water are stirred and mixed evenly to obtain the composition.

[0047] A third objective of this invention is to overcome the shortcomings of existing technologies and provide an application of a composition containing mussel extract in the preparation of cosmetics. Because this composition containing mussel extract has superior whitening, moisturizing, and wrinkle-reducing effects, it can be used in cosmetics.

[0048] The above-mentioned objectives of the present invention are achieved through the following technical measures:

[0049] The above-mentioned composition containing mussel extract is provided for use in the preparation of cosmetics.

[0050] Preferably, the aforementioned cosmetic is a serum, face cream, or face mask.

[0051] This invention discloses a composition containing mussel extract, its preparation method, and its application. The composition comprises mussel extract, *Eucommia ulmoides* extract, *Eucommia ulmoides* male flower extract, triethanolamine, a thickener, a preservative, and deionized water. In the composition containing mussel extract, by weight, the proportions are: mussel extract: 1.50-1.80 parts; *Eucommia ulmoides* extract: 0.20-0.50 parts; *Eucommia ulmoides* male flower extract: 0.50-0.80 parts; triethanolamine: 0.50-0.80 parts; thickener: 0.05-0.10 parts; preservative: 0.10-0.50 parts; and deionized water to a total of 100 parts. The preparation method of the *Eucommia ulmoides* extract is as follows: *Eucommia ulmoides* is extracted with an ethanol-water solution; the extract is concentrated and then extracted with ethyl acetate; the organic phase is separated; and the organic phase is concentrated to obtain the *Eucommia ulmoides* extract. The preparation method of the Eucommia ulmoides male flower extract is as follows: dried Eucommia ulmoides male flowers are extracted with petroleum ether, the extract is concentrated, and then extracted with an ethanol aqueous solution. The extract is then concentrated to obtain the Eucommia ulmoides male flower extract. This invention utilizes a highly efficient synergistic system constructed from mussel extract, septum extract, and Eucommia ulmoides male flower extract. This system not only powerfully combines whitening, moisturizing, and wrinkle-reducing effects, but also significantly reduces the risk of allergies due to the natural and gentle properties of its components. Specifically, the mussel extract forms a moisture-locking barrier on the skin surface, creating a moist base for the subsequent penetration and absorption of active ingredients. Its collagen-promoting effect complements the antioxidant capacity of the active ingredients in the Eucommia ulmoides male flower extract and septum extract. This not only scavenges free radicals to reduce collagen damage but also actively promotes collagen regeneration to fade wrinkles. Simultaneously, it synergistically inhibits melanin activity and reduces pigmentation, while also balancing the moisturizing texture to avoid stickiness or dryness. Detailed Implementation

[0052] The technical solution of the present invention will be further described with reference to the following embodiments. Unless otherwise specified, the raw materials used in the following embodiments are all commercially available products. The mussel extract was purchased from Shenzhen Baiyin Biotechnology Co., Ltd., specifically the MAP-Reno mussel adhesive protein. The boiling range of petroleum ether is 60~90℃.

[0053] Example 1

[0054] A composition containing mussel extract, comprising mussel extract, septum extract, Eucommia ulmoides male flower extract, triethanolamine, thickener, preservative and deionized water.

[0055] In compositions containing mussel extract, by weight,

[0056] Mussel extract: 1.50 parts to 1.80 parts;

[0057] Extract of septum husk: 0.20 to 0.50 parts;

[0058] Eucommia ulmoides male flower extract: 0.50 parts to 0.80 parts;

[0059] Triethanolamine: 0.50 parts to 0.80 parts;

[0060] Thickener: 0.05 parts to 0.10 parts;

[0061] Preservative: 0.10 parts to 0.50 parts;

[0062] Add deionized water to bring the total to 100 parts.

[0063] It should be noted that the thickener of this invention can be carbomer, xanthan gum, etc.; the preservative can be phenoxyethanol, potassium sorbate, etc. The septum of this invention is walnut septum.

[0064] The preparation method of *Pterocarya septum* extract is as follows: *Pterocarya septum* is extracted with an ethanol-water solution, the extract is concentrated, and then extracted with ethyl acetate. The organic phase is separated, and the organic phase is concentrated to obtain the *Pterocarya septum* extract. Specifically, the preparation method of *Pterocarya septum* extract is as follows:

[0065] A1. Crush the septum wood, add an ethanol aqueous solution and heat to extract, filter to obtain the extract, and concentrate the extract to obtain septum wood concentrate.

[0066] A2. The concentrated extract of septum obtained in A1 was added to ethyl acetate and stirred. The organic phase was then separated by standing. The organic phase was then concentrated and dried under vacuum to obtain the septum extract.

[0067] Furthermore, the preparation method of the septum extract is as follows:

[0068] A1. Crush the septum into 20-30 mesh, add 10-16 times the weight of an ethanol aqueous solution with a mass percentage concentration of 30%-50%, and extract at 60-80℃ for 3-5 hours. Filter to obtain the extract, and concentrate the extract to 1 / 3-1 / 4 of its volume to obtain the septum concentrate.

[0069] A2. Add 4 to 8 times the weight of the concentrated septum obtained in A1 to ethyl acetate and stir for 0.5 to 2 hours. Then, allow the mixture to stand to separate the organic phase. Concentrate and dry the organic phase under vacuum at 40 to 50 degrees Celsius to obtain the septum extract.

[0070] The preparation method of Eucommia ulmoides male flower extract is as follows: dried Eucommia ulmoides male flowers are extracted with petroleum ether, the extract is concentrated, and then extracted with an ethanol-water solution. The extract is then concentrated to obtain the Eucommia ulmoides male flower extract. Specifically, the preparation method of Eucommia ulmoides male flower extract is as follows:

[0071] B1. Crush the dried male flowers of Eucommia ulmoides, add petroleum ether, heat and stir to extract, and filter to collect the residue.

[0072] B2. Add the filter residue obtained in B1 to an ethanol-water solution for extraction, separate the extract, and concentrate and dry the extract under vacuum to obtain Eucommia ulmoides male flower extract.

[0073] Furthermore, the preparation method of Eucommia ulmoides male flower extract is as follows:

[0074] B1. Pulverize the dried male flowers of Eucommia ulmoides to 50-80 mesh, add 10-15 times the weight of petroleum ether after pulverization, extract at 40-50℃ for 1-2 hours, filter and take the residue.

[0075] B2. Add the filter residue obtained in B1 to an ethanol aqueous solution with a mass percentage concentration of 40% to 60% and a weight of 10 to 15 times to extract the extract. Separate the extract and concentrate and dry the extract under vacuum to obtain Eucommia ulmoides male flower extract.

[0076] In the vacuum concentration and drying operation of this invention, the vacuum is controlled within the range of absolute pressure of 20 kPa to 50 kPa.

[0077] The composition containing mussel extract is prepared by mixing the above-mentioned amounts of mussel extract, septum extract, Eucommia ulmoides male flower extract, triethanolamine, thickener, preservative and deionized water at room temperature to obtain the composition.

[0078] This composition containing mussel extract utilizes a highly effective synergistic system built from mussel extract, septum extract, and Eucommia male flower extract. This not only powerfully combines whitening, moisturizing, and wrinkle-reducing effects, but also significantly reduces the risk of allergies due to the natural and gentle nature of its ingredients. Specifically, the mussel extract forms a moisture-locking barrier on the skin's surface, creating a moisturized base for the penetration and absorption of subsequent active ingredients. Its collagen-promoting effect complements the antioxidant capabilities of the active ingredients in Eucommia male flower extract and septum extract. This not only eliminates free radicals to reduce collagen damage but also actively promotes collagen regeneration to diminish wrinkles. Simultaneously, it synergistically inhibits melanin activity and reduces pigmentation, while also balancing the moisturizing texture to avoid stickiness or dryness.

[0079] Example 2

[0080] A composition containing mussel extract, which is otherwise identical to that of Example 1, except that it comprises mussel extract, septum extract, Eucommia ulmoides male flower extract, triethanolamine, thickener, preservative and deionized water.

[0081] In the composition containing mussel extract, the total weight parts of the composition are 100 parts, and by weight,

[0082] Mussel extract: 1.50 parts;

[0083] Separator extract: 0.20 parts;

[0084] Eucommia ulmoides male flower extract: 0.80 parts;

[0085] Triethanolamine: 0.50 parts;

[0086] Thickener: 0.10 parts;

[0087] Preservative: 0.10 parts;

[0088] Add deionized water to bring the total to 100 parts.

[0089] In this embodiment, the thickener is carbomer and the preservative is phenoxyethanol.

[0090] The preparation method of the extract of *Septum septum* is as follows:

[0091] A1. Crush the septum into 20 mesh, add 10 times its weight of ethanol aqueous solution with a mass percentage concentration of 30%, extract at 60℃ for 5 hours, filter and collect the extract, and concentrate the extract to 1 / 3 volume to obtain septum concentrate.

[0092] A2. Add 4 times the weight of the concentrated septum obtained in A1 to ethyl acetate and stir for 0.5 h. After standing for 20 min, separate the organic phase. Concentrate and dry the organic phase under vacuum at 50 °C for 2 h to obtain the septum extract.

[0093] The preparation method of Eucommia ulmoides male flower extract is as follows:

[0094] B1. Pulverize the dried male flowers of Eucommia ulmoides to 50 mesh, add 10 times the weight of petroleum ether after pulverization, extract at 40℃ for 2 hours, filter and collect the residue.

[0095] B2. Add the filter residue obtained in B1 to an ethanol aqueous solution with a mass percentage concentration of 40% and add it to a solution at 10 times the weight of the filter residue. Extract the solution at 40°C for 2 hours. Separate the extract and concentrate and dry the extract under vacuum at 50°C for 3 hours to obtain the extract of Eucommia ulmoides male flowers.

[0096] Example 3

[0097] A composition containing mussel extract, which is otherwise identical to that of Example 1, except that it comprises mussel extract, septum extract, Eucommia ulmoides male flower extract, triethanolamine, thickener, preservative and deionized water.

[0098] In the composition containing mussel extract, the total weight parts of the composition are 100 parts, and by weight,

[0099] Mussel extract: 1.80 parts;

[0100] Quartz extract: 0.50 parts;

[0101] Eucommia ulmoides male flower extract: 0.50 parts;

[0102] Triethanolamine: 0.80 parts;

[0103] Thickener: 0.05 parts;

[0104] Preservative: 0.50 parts;

[0105] Add deionized water to bring the total to 100 parts.

[0106] In this embodiment, the thickener is carbomer and the preservative is phenoxyethanol.

[0107] The preparation method of the extract of *Septum septum* is as follows:

[0108] A1. Crush the septum wood to 30 mesh, add 16 times its weight of 50% ethanol aqueous solution, extract at 80℃ for 3 hours, filter and collect the extract, and concentrate the extract to 1 / 4 volume to obtain septum wood concentrate.

[0109] A2. Add 8 times the weight of the concentrated septum obtained in A1 to ethyl acetate and stir for 2 hours. After standing for 20 minutes, separate the organic phase. Concentrate and dry the organic phase under vacuum at 40°C for 2 hours to obtain the septum extract.

[0110] The preparation method of Eucommia ulmoides male flower extract is as follows:

[0111] B1. Pulverize the dried male flowers of Eucommia ulmoides to 80 mesh, add 15 times the weight of petroleum ether after pulverization, extract at 50℃ for 1 hour, filter and collect the residue.

[0112] B2. The filter residue obtained in B1 was added to an ethanol aqueous solution with a mass percentage concentration of 50% and 15 times its weight for extraction. The extraction was carried out at 50°C for 2 hours. The extract was separated and concentrated and dried under vacuum at 50°C for 3 hours to obtain the extract of Eucommia ulmoides male flowers.

[0113] Example 4

[0114] A composition containing mussel extract, which is otherwise identical to that of Example 1, except that it comprises mussel extract, septum extract, Eucommia ulmoides male flower extract, triethanolamine, thickener, preservative and deionized water.

[0115] In the composition containing mussel extract, the total weight parts of the composition are 100 parts, and by weight,

[0116] Mussel extract: 1.68 parts;

[0117] Quartz extract: 0.35 parts;

[0118] Eucommia ulmoides male flower extract: 0.65 parts;

[0119] Triethanolamine: 0.60 parts;

[0120] Thickener: 0.07 parts;

[0121] Preservative: 0.30 parts;

[0122] Add deionized water to bring the total to 100 parts.

[0123] In this embodiment, the thickener is carbomer and the preservative is phenoxyethanol.

[0124] The preparation method of the extract of *Septum septum* is as follows:

[0125] A1. Crush the septum into 25 mesh, add 15 times its weight of ethanol aqueous solution with a mass percentage concentration of 45%, extract at 70℃ for 4 hours, filter and collect the extract, and concentrate the extract to 1 / 4 volume to obtain septum concentrate.

[0126] A2. Add 6 times the weight of the concentrated septum obtained in A1 to ethyl acetate and stir for 1 hour. After standing for 20 minutes, separate the organic phase. Concentrate and dry the organic phase under vacuum at 45°C for 2 hours to obtain the septum extract.

[0127] The preparation method of Eucommia ulmoides male flower extract is as follows:

[0128] B1. Pulverize the dried male flowers of Eucommia ulmoides to 60 mesh, add 13 times the weight of petroleum ether after pulverization, extract at 45℃ for 1.5h, filter and collect the residue.

[0129] B2. The filter residue obtained in B1 was added to an ethanol aqueous solution with a mass percentage concentration of 50% and 12 times its weight for extraction. The extraction was carried out at 45°C for 2 hours. The extract was separated and concentrated and dried under vacuum at 50°C for 3 hours to obtain the extract of Eucommia ulmoides male flowers.

[0130] Comparative Example 1

[0131] A composition containing mussel extract, otherwise identical in characteristics to Example 4, except that: in the composition containing mussel extract, the total weight parts of the composition are 100 parts, by weight.

[0132] Mussel extract: 0.60 parts;

[0133] Quartz extract: 0.80 parts;

[0134] Eucommia ulmoides male flower extract: 1.00 part;

[0135] Triethanolamine: 0.60 parts;

[0136] Thickener: 0.07 parts;

[0137] Preservative: 0.30 parts;

[0138] Add deionized water to bring the total to 100 parts.

[0139] Comparative Example 2

[0140] A composition containing mussel extract, otherwise identical in characteristics to Example 4, except that: in the composition containing mussel extract, the total weight parts of the composition are 100 parts, by weight.

[0141] Mussel extract: 2.00 parts;

[0142] Quartz extract: 0.10 parts;

[0143] Eucommia ulmoides male flower extract: 0.20 parts;

[0144] Triethanolamine: 0.60 parts;

[0145] Thickener: 0.07 parts;

[0146] Preservative: 0.30 parts;

[0147] Add deionized water to bring the total to 100 parts.

[0148] Comparative Example 3

[0149] A composition containing mussel extract, otherwise identical in characteristics to Example 4, except that: in the composition containing mussel extract, the total weight parts of the composition are 100 parts, by weight.

[0150] Mussel extract: 2.68 parts;

[0151] Separator extract: 0 parts;

[0152] Eucommia ulmoides male flower extract: 0 parts;

[0153] Triethanolamine: 0.60 parts;

[0154] Thickener: 0.07 parts;

[0155] Preservative: 0.30 parts;

[0156] Add deionized water to bring the total to 100 parts.

[0157] Comparative Example 4

[0158] A composition containing mussel extract, otherwise identical in characteristics to Example 4, except that: in the composition containing mussel extract, the total weight parts of the composition are 100 parts, by weight.

[0159] Mussel extract: 1.68 parts;

[0160] Quartz extract: 1.00 part;

[0161] Eucommia ulmoides male flower extract: 0 parts;

[0162] Triethanolamine: 0.60 parts;

[0163] Thickener: 0.07 parts;

[0164] Preservative: 0.30 parts;

[0165] Add deionized water to bring the total to 100 parts.

[0166] Comparative Example 5

[0167] A composition containing mussel extract, otherwise identical in characteristics to Example 4, except that: in the composition containing mussel extract, the total weight parts of the composition are 100 parts, by weight.

[0168] Mussel extract: 1.68 parts;

[0169] Separator extract: 0 parts;

[0170] Eucommia ulmoides male flower extract: 1.00 part;

[0171] Triethanolamine: 0.60 parts;

[0172] Thickener: 0.07 parts;

[0173] Preservative: 0.30 parts;

[0174] Add deionized water to bring the total to 100 parts.

[0175] Comparative Example 6

[0176] A composition containing mussel extract, with other characteristics the same as in Example 4, except that: the preparation method of the mussel septum extract is to crush the mussel septum to 25 mesh, add 15 times the weight of an aqueous ethanol solution with a mass percentage concentration of 70% after crushing, extract at 70°C for 4 hours, filter to obtain the extract, and vacuum concentrate and dry the extract at 50°C for 4 hours to obtain the mussel septum extract.

[0177] Comparative Example 7

[0178] A composition containing mussel extract, with other characteristics the same as in Example 4, except that: the preparation method of Eucommia ulmoides male flower extract is to pulverize dried Eucommia ulmoides male flowers to 60 mesh, add 12 times the weight of an aqueous ethanol solution with a mass percentage concentration of 70% for extraction, separate the extract, and vacuum concentrate and dry the extract at 50°C for 3 hours to obtain Eucommia ulmoides male flower extract.

[0179] Comparative Example 8

[0180] A composition containing mussel extract, otherwise identical to that of Example 4, except that the preparation method of Eucommia ulmoides male flower extract is as follows:

[0181] B1. Pulverize the dried male flowers of Eucommia ulmoides to 60 mesh, add 13 times the weight of petroleum ether after pulverization, extract at 45℃ for 1.5h, filter and collect the residue.

[0182] B2. Add the filter residue obtained in B1 to an ethanol aqueous solution with a mass percentage concentration of 90% (12 times its weight) and extract at 45°C for 2 hours. Separate the extract and concentrate and dry the extract under vacuum at 50°C for 3 hours to obtain Eucommia ulmoides male flower extract.

[0183] Comparative Example 9

[0184] A composition containing mussel extract, which is otherwise identical to that of Example 4, except that the extract of Eucommia ulmoides male flowers is replaced with extract of Eucommia ulmoides leaves.

[0185] The preparation method of Eucommia ulmoides leaf extract is as follows:

[0186] B1. Pulverize the dried Eucommia ulmoides leaves to 60 mesh, add 13 times the weight of petroleum ether after pulverization, extract at 45℃ for 1.5 hours, filter and collect the residue.

[0187] B2. The filter residue obtained in B1 was added to an aqueous ethanol solution with a mass percentage concentration of 50% and 12 times its weight for extraction. The extract was separated and concentrated and dried under vacuum at 50°C for 3 hours to obtain Eucommia ulmoides leaf extract.

[0188] Comparative Example 10

[0189] A composition containing mussel extract, which is otherwise identical to that of Example 4, except that the extract of Eucommia ulmoides male flowers is replaced with extract of Eucommia ulmoides bark.

[0190] The preparation method of Eucommia bark extract is as follows:

[0191] B1. Pulverize the dried Eucommia bark to 60 mesh, add 13 times its weight of petroleum ether after pulverization, extract at 45℃ for 1.5 hours, filter and collect the residue.

[0192] B2. Add the filter residue obtained in B1 to an ethanol aqueous solution with a mass percentage concentration of 50% (12 times its weight) for extraction. Separate the extract and concentrate and dry the extract under vacuum at 50°C for 3 hours to obtain Eucommia bark extract.

[0193] Test case

[0194] 1. Safety evaluation

[0195] Two hundred and sixty women aged 20 to 40 years were randomly selected. These volunteers all had capsaicin-positive sensitive skin. The 260 sensitive skin volunteers were randomly divided into 13 groups of 20 people each. After cleansing each day, 0.15g of the compositions from Examples 2 to 4 and Comparative Examples 1 to 10 were applied to the entire face, avoiding sensitive areas such as the eyes and lips, and massaged in. After 28 days, according to the requirements for adverse skin reaction results in the "Cosmetic Safety Technical Specifications - 2015 Edition", dermatologists scored the skin condition of the sensitive skin volunteers and counted the number of people who experienced irritation reactions (such as erythema, papules, blisters, edema, etc.). The data are shown in Table 1.

[0196] Table 1. Number of people experiencing a stimulus response

[0197]

[0198] As shown in Table 1, in the tests on people with sensitive skin who are positive for capsaicin, no irritation reaction was observed in Examples 2-4, Comparative Examples 1-5, and Comparative Examples 8-10. Only in Comparative Examples 6 and 7, 4 people and 1 person, respectively, experienced irritation. This indicates that the mussel extract, septum extract, and Eucommia ulmoides male flower extract in the composition of the present invention, after being prepared and compounded according to a specific method, have good biocompatibility and remain mild even on sensitive skin, with an extremely low risk of allergies.

[0199] 2. Anti-wrinkle efficacy experiment

[0200] Volunteers aged 35-55 years with similar degrees of facial wrinkles and no history of skin allergies were randomly divided into 13 groups of 20 participants each. After cleansing their faces, participants applied the compositions of Examples 2-4 and Comparative Examples 1-10 evenly to their faces, avoiding sensitive areas such as the eyes and lips, and gently massaged until absorbed. They used 0.15g twice daily, morning and evening, for 30 consecutive days. The improvement rate of facial wrinkles was tested after 30 days. Facial wrinkle conditions were measured using a Skin Visiometer SV600 system, and the average value for each group was calculated. Facial wrinkle improvement rate (%) = (Average wrinkle value in each group after using the examples / comparative examples - Average wrinkle value in each group before use) / Average wrinkle value in each group before use. The wrinkle improvement rate in the table is 100%, which is calculated by the formula and then taken as a positive value. The data is shown in Table 2.

[0201] Table 2. Improvement rate of facial wrinkles

[0202]

[0203] As shown in Table 2, the facial wrinkle improvement rates of Examples 2 to 4 were 62.8%, 65.5%, and 68.3%, respectively, all higher than the respective comparison ratios, with Example 4 showing the best effect. The comparison reveals that the synergistic effect of the three extracts—mussel extract, septum extract, and eucommia male flower extract—is superior to combinations lacking a single ingredient or with unbalanced proportions, indicating that the three extracts form a highly efficient complement in promoting collagen synthesis and reducing free radical damage.

[0204] 3. Whitening efficacy experiment

[0205] Volunteers aged 23-50 with relatively dull facial skin were randomly divided into 13 groups of 20 each. Before the test, all volunteers cleaned and dried their inner forearms. A 5cm x 5cm area was measured on the inner forearm as the test area, with each test area spaced 1cm apart. Data on melanin and color difference were collected and analyzed. Skin color changes were measured using the Lab colorimetric system specified by the International Commission on Illumination (CIE), and the data were used as the skin background value. Subjects used the compositions of Examples 2-4 and Comparative Examples 1-10 twice daily, morning and evening, at 0.05g each time, for 4 consecutive weeks. Changes in skin brightness and melanin content were measured before and after the test. Melanin ΔMI = T - T0; where T is the final value of the test area; T0 is the initial value of the test area; and skin brightness ΔL... =L-L0; where L is the final value of the test area; L0 is the initial value of the test area, and the data are shown in Table 3.

[0206] Table 3. Data on skin brightness and skin melanin

[0207]

[0208] As shown in Table 3, the whitening effect of the compositions of the present invention is outstanding, with a significant reduction in melanin. The skin melanin ΔMI values ​​in Examples 2 to 4 are -21.63, -21.61, and -22.25, respectively, all superior to most comparative examples, with Example 4 showing the most significant effect. The skin tone brightening effect is also good; the skin brightness ΔL in Examples 2 to 4 is significantly improved. The values ​​were 1.80, 1.83, and 1.94, respectively, which were also higher than those of comparative examples 1 to 10. This indicates that the three extracts, mussel extract, septum extract, and eucommia male flower extract, can work together to more comprehensively inhibit melanin activity, reduce pigmentation, and improve skin brightness.

[0209] 4. Moisturizing effect experiment

[0210] Volunteers aged 30-45 years were selected and met the following criteria: no allergy to cosmetics, no acute facial inflammation, no use of hormonal drugs or immunosuppressants, no participation in other clinical trials, and no application of topical preparations. They were randomly divided into 13 groups of 10 people each. After daily cleansing, each volunteer used 0.15g of the compositions provided in Examples 2-4 and Comparative Examples 1-10, massaging it onto the entire face, avoiding sensitive areas such as the eyes and lips, for two consecutive months (60 days). The skin moisture content of the volunteers' facial cheek area was measured using a skin moisture meter (CM825). The increase in skin moisture content was calculated using the following formula: Increase in skin moisture content = Skin moisture content after use - Skin moisture content before use. The data are shown in Table 4.

[0211] Table 4. Data on the increase in skin moisture content

[0212]

[0213] As shown in Table 4, the skin moisture content of Examples 2-4 increased by 7.3%-7.5%, significantly higher than the comparative examples, with Example 4 showing the best performance. This is attributed to the water-locking barrier effect of the mussel extract and the synergistic moisturizing effect of the other two extracts, which can effectively increase the moisture content of the stratum corneum, and the effect is better than the combination of missing components or simplified extraction process.

[0214] In summary, the composition of the present invention achieves a balance between safety and efficacy through the scientific compounding of three core extracts and a specific extraction process. Under the premise of being gentle and non-irritating, it synergistically exerts excellent wrinkle-reducing, whitening, and moisturizing effects, and the overall performance of the optimal ratio is the most outstanding.

[0215] Example 5

[0216] The application of a composition containing mussel extract, as described in any one of Examples 2 to 4, in the preparation of cosmetics, wherein the cosmetics are serums, creams, masks, etc.

[0217] As can be seen from the efficacy data, applying the compositions of Examples 2 to 4 to cosmetics can fully leverage their excellent performance in whitening, moisturizing, and wrinkle reduction, while also ensuring gentleness and safety. In cosmetic applications, this composition provides multiple layers of care for the skin through the synergistic effect of mussel extract, septum extract, and Eucommia male flower extract: it can effectively improve skin wrinkles and enhance skin elasticity by utilizing the water-locking barrier and collagen synthesis-promoting effects of mussel extract, combined with the antioxidant capabilities of Eucommia male flower extract and septum extract; it can also inhibit melanin activity, reduce pigmentation, and brighten skin tone through the synergistic effect of the three, achieving a whitening effect; at the same time, its good moisturizing properties can increase the water content of the stratum corneum, improve dry skin, and the ingredients are natural and gentle, reducing the risk of allergies even for people with capsaicin-positive sensitive skin, ensuring safety in use.

[0218] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A composition containing mussel extract, characterized in that, It is composed of mussel extract, septum extract, Eucommia ulmoides male flower extract, triethanolamine, thickener, preservative and deionized water; In the composition containing mussel extract, by weight, Mussel extract: 1.50 parts to 1.80 parts; Extract of septum husk: 0.20 to 0.50 parts; Eucommia ulmoides male flower extract: 0.50 parts to 0.80 parts; Triethanolamine: 0.50 parts to 0.80 parts; Thickener: 0.05 parts to 0.10 parts; Preservative: 0.10 parts to 0.50 parts; Add deionized water to bring the total to 100 parts; The preparation method of the extract of the septum is as follows: A1. Crush the septum into 20-30 mesh, add 10-16 times the weight of an ethanol aqueous solution with a mass percentage concentration of 30%-50%, and extract at 60-80℃ for 3-5 hours. Filter to obtain the extract, and concentrate the extract to 1 / 3-1 / 4 of its volume to obtain the septum concentrate. A2. Add the concentrated septum obtained in A1 to 4 to 8 times its weight of ethyl acetate and stir for 0.5 to 2 hours. After standing, separate the organic phase and concentrate and dry the organic phase under vacuum at 40 to 50 degrees Celsius to obtain the septum extract. The preparation method of the Eucommia ulmoides male flower extract is as follows: B1. Pulverize the dried male flowers of Eucommia ulmoides to 50-80 mesh, add 10-15 times the weight of petroleum ether after pulverization, extract at 40-50℃ for 1-2 hours, filter and take the residue. B2. Add the filter residue obtained in B1 to an ethanol aqueous solution with a mass percentage concentration of 40% to 60% and a weight of 10 to 15 times to extract the extract. Separate the extract and concentrate and dry the extract under vacuum to obtain Eucommia ulmoides male flower extract.

2. The composition containing mussel extract according to claim 1, characterized in that, In the composition containing mussel extract, by weight, Mussel extract: 1.68 parts; Quartz extract: 0.35 parts; Eucommia ulmoides male flower extract: 0.65 parts; Triethanolamine: 0.60 parts; Thickener: 0.07 parts; Preservative: 0.30 parts; Add deionized water to bring the total to 100 parts.

3. The composition containing mussel extract according to claim 2, characterized in that, The preparation method of the extract of the septum is as follows: A1. Crush the septum into 25 mesh, add 15 times its weight of ethanol aqueous solution with a mass percentage concentration of 45%, extract at 70℃ for 4 hours, filter and collect the extract, and concentrate the extract to 1 / 4 volume to obtain septum concentrate. A2. The concentrated extract of septum obtained in A1 was added to 6 times its weight of ethyl acetate and stirred for 1 hour. The organic phase was then separated by standing. The organic phase was concentrated and dried under vacuum at 45°C to obtain the septum extract.

4. The composition containing mussel extract according to claim 3, characterized in that, The preparation method of the Eucommia ulmoides male flower extract is as follows: B1. Pulverize the dried male flowers of Eucommia ulmoides to 60 mesh, add 13 times the weight of petroleum ether after pulverization, extract at 45℃ for 1.5h, filter and collect the residue. B2. Add the filter residue obtained in B1 to an ethanol aqueous solution with a mass percentage concentration of 50% (12 times its weight) for extraction, separate the extract, and concentrate and dry the extract under vacuum to obtain Eucommia ulmoides male flower extract.

5. A method for preparing a composition containing mussel extract as described in any one of claims 1 to 4, characterized in that: The mussel extract, the septum extract, the eucommia male flower extract, triethanolamine, thickener, preservative and deionized water were stirred and mixed evenly to obtain the composition.

6. The use of the composition containing mussel extract as described in any one of claims 1 to 4 in the preparation of cosmetics; The cosmetic product is a serum, face cream, or face mask.

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