一种呈递Stoppin肽的细菌外膜囊泡及其构建方法和应用
By presenting Stoppin peptide on the surface of bacterial outer membrane vesicles, W-Stoppin OMV was constructed, which solved the problem of insufficient cell penetration of Stoppin peptide, and achieved effective delivery to tumor cells and significant anti-tumor effects, especially tumor cell migration inhibition, invasion inhibition and apoptosis promotion.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- THE FIRST AFFILIATED HOSPITAL OF HENAN UNIV OF TCM
- Filing Date
- 2025-07-24
- Publication Date
- 2026-07-17
AI Technical Summary
Stoppin peptides have limited cell penetration and low delivery efficiency, making it difficult for them to effectively exert their anti-tumor effects. Existing nanocarrier systems suffer from drawbacks such as high cytotoxicity, short half-life, and poor targeting, and there is a lack of effective delivery carriers.
By using genetic engineering technology to present Stoppin peptides on the surface of bacterial outer membrane vesicles, engineered bacterial outer membrane vesicles W-Stoppin OMVs were constructed to target the presentation of Stoppin peptides. W-Stoppin OMVs were then prepared by displaying ClyA-Stoppin fusion protein on the surface of OMVs.
It significantly inhibits the invasion and migration of tumor cells and promotes tumor cell apoptosis. Compared with Stoppin monopeptide, it exhibits superior anti-tumor effects and has significant tumor cell migration inhibition, invasion inhibition and apoptosis-promoting effects.
Smart Images
Figure CN121059841B_ABST