用于合成5’-加帽RNA的组合物和方法
By using specific starting oligonucleotide primers and RNA polymerase transcription, the inefficiency and difficulties of capped RNA synthesis in existing technologies have been solved, achieving efficient and simplified capped RNA synthesis suitable for large-scale production.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- TRILINK BIOTECH LLC
- Filing Date
- 2016-09-20
- Publication Date
- 2026-07-17
AI Technical Summary
Existing technologies for synthesizing capped RNA in vitro suffer from problems such as being laborious, inefficient, difficult to control, having low yields, producing many isoforms, and requiring additional enzymatic reactions and purification steps. In particular, it is difficult to achieve efficient synthesis of mRNA with cap 1 and cap 2 structures in large-scale production.
Using specific starting oligonucleotide primers, including the I structure, capped RNA is synthesized by transcription on a polynucleotide template via RNA polymerase, avoiding bidirectional initiation, improving yield and simplifying the purification process. Chemically modified nucleotides are used to ensure the formation of cap 1 and cap 2 structures.
This invention provides an efficient and simplified method for capped RNA synthesis, which improves yield, reduces isoform generation, lowers costs, and simplifies purification steps, making it suitable for large-scale production.
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Figure CN121064267B_ABST