A method for constructing an acacia hairy root transgenic system mediated by agrobacterium

By using Agrobacterium rhizogenes-mediated methods, the hairy root induction process of Acacia confusa was optimized, solving the problems of contamination and unstable seedling quality during tissue culture. This achieved efficient transgenic hairy root induction and provided stable materials for genetic trait improvement and gene function verification.

CN121087093BActive Publication Date: 2026-07-14RES INST OF TROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2025-10-13
Publication Date
2026-07-14

AI Technical Summary

Technical Problem

In existing technologies, the tissue culture process of Acacia confusa suffers from serious pollution, difficulty in removing endophytic bacteria, and easy vitrification and browning, resulting in unstable seedling quality. Furthermore, the traditional Agrobacterium tumefaciens-mediated genetic transformation system relies on tissue culture seedlings, leading to aging of genetic material and DNA methylation, which affects the effectiveness of transgenic research.

Method used

Using Agrobacterium rhizogenes-mediated methods, plant expression vectors containing the target gene were transferred into Ar.1193 Agrobacterium rhizogenes to prepare infection solutions, which were then used to infect stem buds and cotyledon explants of Acacia confusa. Hairy roots were then induced in a specific culture medium. Specific combinations of antibacterial agents and antibiotics were used to optimize the induction conditions to improve the induction efficiency of hairy roots.

Benefits of technology

Hairy roots and transgenic positive hairy roots were successfully induced in acacia species such as blackwood acacia, fringe acacia, majan acacia, tufted acacia, large-leaved acacia, and Taiwan acacia, improving transformation efficiency and providing ideal materials for genetic trait improvement and gene function verification.

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Abstract

The present application relates to the field of plant transgenic technology, in particular to a method for constructing a transgenic system of Acacia hairy root mediated by Agrobacterium rhizogenes.The present application uses the stem buds and cotyledons of several Acacia species of Leguminosae as explant materials, and establishes a transgenic system mediated by Agrobacterium rhizogenes, which is efficient, stable and suitable for multiple tree species of Acacia.The method provided by the present application can realize stable transformation of target genes on roots within 3-5 weeks, and has successfully induced hairy roots and transgenic positive hairy roots on Acacia melanoxylon, A. pendula, A. mangium, A. crassicarpa, A. auriculiformis, A. crassicarpa and A. taiwaniana, thereby providing ideal materials for the study of root system theory and the synthesis pathway of specific secondary metabolites of multiple tree species of Acacia, and providing technical support for the improvement of multiple tree species of Acacia and the verification of gene function.
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Citation Information

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