An application method for preventing and treating cotton verticillium wilt by using terbinafine to target sterol binding protein VdPBP1

CN121100718BActive Publication Date: 2026-06-19NANJING FORESTRY UNIV

Patent Information

Authority / Receiving Office: CN · China
Patent Type: Patents(China)
Current Assignee / Owner: NANJING FORESTRY UNIV
Filing Date: 2025-09-16
Publication Date: 2026-06-19

AI Technical Summary

Technical Problem

Existing cotton Verticillium wilt control technologies suffer from problems such as high pesticide concentrations, poor sustained effects, unclear mechanisms of action, and high costs. Existing pesticides, such as shenqinmycin and benomyl, have high half-inhibitory concentrations, leading to high control costs and the pathogen's tendency to develop drug resistance.

Method used

Terbinafine was used to target the sterol-binding protein VdPBP1 to control cotton Verticillium wilt. The half-maximal inhibitory concentration (EC50) of terbinafine was determined to be 10.8 ng/mL through plate inhibition assays. Application regimens for prevention and treatment were developed, and the control effect was verified by HPLC and RT-qPCR. The mechanism of action between terbinafine and VdPBP1 was elucidated, including target identification, functional verification, and interaction verification of VdPBP1.

Benefits of technology

It achieves efficient control of cotton Verticillium wilt at low concentrations, reduces pesticide dosage and cost, avoids non-target effects, covers the entire growth period for control, and makes it difficult for pathogens to develop drug resistance.

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Abstract

This invention discloses an application method for controlling cotton Verticillium wilt using terbinafine targeting the sterol-binding protein VdPBP1 of Verticillium dahliae, relating to the field of cotton control technology. The method includes the following application steps: S1, confirmation of terbinafine's chemical properties; S2, development of a terbinafine application plan; S3, verification and assurance of control efficacy. This invention provides theoretical support for precision control; and the EC50 of terbinafine... 50 With a concentration of 10.8 ng / mL, the dosage can be significantly reduced, and the unit price of terbinafine is 30.4 yuan / g, making large-scale application more cost-effective. Moreover, terbinafine only binds to VdPBP1 of Verticillium dahliae, blocking its mediated electron transfer. Existing agents such as shenqinmycin inhibit histone acetylation, and benomyl interferes with a wide range of microtubule targets, easily affecting non-target organisms. Furthermore, the ergosterol synthesis pathway is an essential pathway for fungi, and VdERG1 is a single-copy gene in Verticillium dahliae, making it difficult for pathogens to develop drug resistance through gene mutation. The control points are broad, covering the entire growth period.

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