Cydonia oblonga tissue culture seedling double-layer rooting and seedling strengthening culture medium and cydonia oblonga tissue culture seedling rooting and seedling hardening method

Abstract

The invention provides a cydonia oblonga tissue culture seedling double-layer rooting and seedling strengthening culture medium and a cydonia oblonga tissue culture seedling rooting and seedling hardening method, and belongs to the technical field of plant tissue culture. The upper layer of the double-layer rooting and seedling strengthening culture medium comprises 1 / 2 MS, 6.8-7.2 g / L of agar, 28-32 g / L of sucrose and 0.8-1.2 mg / L of NAA; and the lower layer comprises MS, 6.8-7.2 g / L of agar, 28-32 g / L of sucrose and 0.4-0.6 mg / L of 6-BA. According to the composite culture medium system with the upper layer and the lower layer, two key stages of rooting and seedling strengthening are connected in time and separated in space, accurate time sequence regulation and control of two key development stages of rooting and seedling strengthening of tissue culture seedlings are achieved, the problems that the seedlings are prone to withering after rooting and the roots are thick and suberiform in a traditional single-layer culture medium are solved, the rooting rate can be increased, and the yield is increased. The transplanting survival rate is increased, and the production efficiency is remarkably improved.

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