Method for improving stability of self-assembly function of hydrophobic active substance loaded protein through natural deep eutectic

Abstract

The invention discloses a method for improving stability of a self-assembly function of hydrophobic active substance loaded protein through natural deep eutectic, and relates to the technical field of functional food processing. A natural deep eutectic solvent is prepared from glycerin and choline chloride according to the molar ratio of 1: 1, the isoorientin is dissolved in the natural deep eutectic solvent to improve the solubility of the natural deep eutectic solvent, then the natural deep eutectic solvent is added into beta-lactoglobulin to be self-assembled with the isoorientin, the beta-lactoglobulin is encapsulated in the beta-lactoglobulin by means of a unique hydrophobic cavity structure of the beta-lactoglobulin, and therefore the biological activity of the isoorientin is improved. Compared with a traditional method, glycerin-choline chloride is adopted as a natural deep eutectic solvent, the solubility of isoorientin can be remarkably improved, meanwhile, beta-lactoglobulin can be encapsulated in a hydrophobic cavity to keep stability, and the solvent is environmentally friendly and low in toxicity.

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