A signal transmission device and a signal acquisition device for a microelectrode culture dish

Abstract

The application relates to the technical field of detection equipment, and discloses a signal transmission device and a signal acquisition device for a microelectrode culture dish, which comprises a probe mounting plate on one side of a circuit board; each probe group in a plurality of probe groups comprises five probes which are arranged in a cross shape and have a first interval; each probe comprises a probe head and a probe sleeve; one probe sleeve penetrates through the probe mounting plate; one end of the probe sleeve exceeds the probe mounting plate and is away from the one side of the circuit board; the other end of the probe sleeve is welded to the circuit board; the probe head is inserted into the probe sleeve; and a connecting plug is connected to the circuit board. The circuit board is placed under the probe mounting plate, and the probe sleeve penetrates through the probe mounting plate, so that the probe sleeve is fixed, and the overall stability of the probe is improved; the probe is divided into the probe sleeve and the probe head, one end of the probe sleeve penetrating through the probe mounting plate is welded to the circuit board, and the probe head is inserted into the probe sleeve, so that the probe head can be quickly replaced, and the convenience of maintenance is improved.

Description

Technical Field

[0001] This application relates to the field of detection equipment technology, and in particular to a signal transmission device and a signal acquisition device for microelectrode culture dishes. Background Technology

[0002] In numerous fields such as cell electrophysiology research, drug screening, and biomedical detection, the demand for high-throughput and stable signal measurement in cell microelectrode culture dishes is becoming increasingly urgent. Traditional cell microelectrode measurement techniques face many challenges and struggle to meet the high-precision and high-efficiency requirements of modern scientific research and applications.

[0003] Traditional cell electrophysiological recording methods struggle to achieve long-term measurements and simultaneous multi-site measurements of cellular networks. While patch-clamp techniques are considered the "gold standard" for detecting cellular electrical signals, they are extremely difficult to operate, require highly experienced personnel, have low throughput, are costly, and are invasive, causing some damage to cells. Although multi-electrode array (MEA) technology has been developed for many years and has applications in detecting the electrophysiological activity of cardiomyocytes and neurons, existing MEA devices are time-consuming and labor-intensive to maintain.

[0004] In summary, there is a need to provide a signal transmission device and signal acquisition device for microelectrode culture dishes that are easy to maintain. Summary of the Invention

[0005] This application proposes a signal transmission device and a signal acquisition device for microelectrode culture dishes, which can improve the convenience of device maintenance.

[0006] In a first aspect, this application proposes a signal transmission device for a microelectrode culture dish, comprising: multiple probe groups, a probe mounting plate, a circuit board, and a connector plug;

[0007] The probe mounting plate is located on one side of the circuit board;

[0008] Each of the plurality of probe groups includes five probes arranged in a cross pattern with a first interval between them;

[0009] Each of the probes includes a probe head and a probe sleeve;

[0010] One of the probe sleeves passes through the probe mounting plate; one end of the probe sleeve extends beyond the probe mounting plate to the side facing away from the circuit board; the other end of the probe sleeve is soldered to the circuit board.

[0011] The probe head is inserted into the probe sleeve;

[0012] The probe group is used to connect to the signal output terminal of the microelectrode culture dish and receive the signal from the microelectrode culture dish;

[0013] The connector plug is connected to the circuit board and is used to transmit the signals received by the probe group.

[0014] Preferably, it further includes: multiple elastic positioning posts and multiple positioning pins;

[0015] One end of each of the plurality of elastic positioning posts is fixed to the circuit board, and the other end passes through the probe mounting plate;

[0016] One end of each of the plurality of positioning pins is fixed to the side of the probe mounting plate opposite to the circuit board.

[0017] Preferably, it further includes: a culture dish base; the culture dish base is placed on the other end of the plurality of elastic positioning posts.

[0018] Preferably, it further includes: a housing; the culture dish base, multiple probe groups, probe mounting plate, circuit board and connector are all placed inside the housing.

[0019] Preferably, it further includes: a top cover; one long side of the top cover is connected to one long side of the outer shell via a connecting portion.

[0020] Preferably, it further includes: a locking structure; the locking structure includes a locking sleeve and a sliding lock;

[0021] The locking sleeve is fixed to one side of the other long side of the upper cover, and the sliding lock is fixed to one side of the other long side of the outer shell; or

[0022] The locking sleeve is fixed to one side of the other long side of the outer shell, and the sliding lock is fixed to one side of the other long side of the upper cover.

[0023] Preferably, the first interval is 2.54 mm.

[0024] Preferably, the probe head and the probe sleeve are made of gold-plated stainless steel.

[0025] Preferably, the probe mounting plate is made of bakelite.

[0026] Secondly, this application also provides a signal acquisition device for a microelectrode culture dish, including a signal transmission device for a microelectrode culture dish as described in any one of the first aspects, and further including a signal measurement circuit; the signal measurement circuit is placed on the circuit board and is electrically connected to the plurality of probe groups and the connector plug.

[0027] The advantages of this application are as follows: by placing the circuit board under the probe mounting plate and having the probe sleeve pass through the probe mounting plate, the probe sleeve is fixed, thereby improving the overall stability of the probe; by dividing the probe into a probe sleeve and a probe head, one end of the probe sleeve that passes through the probe mounting plate is soldered to the circuit board, and the probe head is inserted into the probe sleeve, thereby enabling quick replacement of the probe head and improving the convenience of maintenance. Attached Figure Description

[0028] Various other advantages and benefits will become apparent to those skilled in the art upon reading the following detailed description of preferred embodiments. The accompanying drawings are for illustrative purposes only and are not intended to limit the scope of this application. Furthermore, the same reference numerals denote the same parts throughout the drawings. In the drawings:

[0029] Figure 1 This is a schematic diagram of a signal transmission device for a microelectrode culture dish provided in this application;

[0030] Figure 2 This is a schematic diagram of the arrangement of multiple probes in a probe group for a signal transmission device for a microelectrode culture dish provided in this application;

[0031] Figure 3 This is a schematic diagram of the arrangement of multiple probes in a probe group for another signal transmission device for microelectrode culture dishes provided in this application;

[0032] Figure 4 This is a schematic diagram of the connection of the signal output terminal of a cell microelectrode culture dish for a signal transmission device for a microelectrode culture dish provided in this application;

[0033] Figure 5 This is a schematic diagram of an elastic positioning column and positioning pin for a signal transmission device used in a microelectrode culture dish, as provided in this application.

[0034] Figure 6 This is a schematic diagram of the top cover of a signal transmission device for a microelectrode culture dish provided in this application being opened;

[0035] Figure 7 This is a schematic diagram of the closing of the top cover of a signal transmission device for a microelectrode culture dish provided in this application;

[0036] Figure 8 This is a schematic diagram of the housing of a signal transmission device for a microelectrode culture dish provided in this application. Detailed Implementation

[0037] Exemplary embodiments of the present disclosure will now be described in more detail with reference to the accompanying drawings. While exemplary embodiments of the present disclosure are shown in the drawings, it should be understood that the present disclosure may be implemented in various forms and should not be limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the disclosure to those skilled in the art.

[0038] Firstly, according to embodiments of this application, a signal transmission device for microelectrode culture dishes is proposed, such as... Figure 1 As shown, the device includes: multiple probe groups 100, a probe mounting plate 200, a circuit board (PCB) 300, and a connector 400. The probe mounting plate 200 is located on one side of the PCB 300. Each probe group 100 includes five probes 101 spaced apart by a first interval D1 and arranged in a staggered pattern. Each probe 101 includes a probe head 110 and a probe sleeve 120. A probe sleeve 120 passes through the probe mounting plate 200. One end of the probe sleeve 120 extends beyond the probe mounting plate 200 and faces away from the PCB 300. The other end of the probe sleeve 120 is soldered to the PCB 300. The probe head 110 is inserted into the probe sleeve 120. The probe group 100 is used to connect to the signal output terminal of a microelectrode culture dish to receive signals from the microelectrode culture dish. The connector 400 is connected to the circuit board and is used to transmit the signals received by the probe group 100.

[0039] like Figure 2 As shown, the first interval D1 is 2.54 mm. The probe head 110 and probe sleeve 120 are made of gold-plated stainless steel. The gold-plated stainless steel includes 6061 aluminum alloy. The probe mounting plate 200 is made of bakelite. The standardized layout of the probes 101 (24 mirrored interfaces, adaptable to microelectrode culture dishes with up to 96 wells) allows probe groups 100 or multiple probe groups 100 to form an integrated probe array module.

[0040] For the replacement of probe tip 110, quick replacement can be achieved by plugging and unplugging the integrated small probe modules. Probe 101 can also be replaced by a reed module, thereby enabling signal export from the cell microelectrode culture dish.

[0041] Regarding the compatibility of devices with different microplates, existing technologies suffer from inconsistent and disorganized signal interface specifications, lacking a universal design. Consequently, a single measurement device often only fits specific types or numbers of wells in cell microelectrode culture dishes, making it difficult to apply flexibly in diverse laboratory experiments. For example, when faced with cell microelectrode culture dishes with different functions, or when experiments need to be performed simultaneously on culture dishes (microelectrode culture dishes) with different numbers of wells, existing devices cannot meet the requirements, exhibiting extremely poor compatibility. Furthermore, traditional devices are cumbersome to operate, severely limiting the diversity and efficiency of experiments.

[0042] In terms of structural design, existing technologies lack specific optimizations for high-throughput measurements. The probe array layout is unreasonable, making it difficult to adapt to multi-well cell microelectrode culture dishes such as 96-well dishes, and it also fails to meet the requirements for synchronous signal acquisition. This results in the inability to accurately and efficiently acquire the electrical signals of cells in each well during high-throughput experiments. Furthermore, due to the low integration of the signal processing module and the probe array, the signal is highly susceptible to interference from the external environment and internal components during transmission, severely affecting signal stability and accuracy. This makes it difficult to achieve synchronous and stable signal measurement across multiple wells, significantly limiting its application in large-scale cell experiments.

[0043] like Figure 3 As shown, the embodiment of this application uses an array probe module (probe group 100) for overall acquisition, with a cross-arrangement of the standardized interface spacing (first interval D1) of 2.54 mm, achieving an interface of five probes per group. A total of 24 mirror interfaces are set on both sides (long side) of the probe mounting plate 200 using a mirroring method. The combination of these 24 groups allows signal export from cell microelectrode culture dishes with up to 96 wells. Furthermore, it can adapt to cell microelectrode culture dishes with different functions and the same interface design, including culture dishes with up to 96 wells. The base has good versatility, greatly improving the versatility and compatibility of the device, and can be used for different measurement needs, meeting various experimental requirements in the laboratory. For example, Figure 4 As shown, the signal output terminal 10 of the cell microelectrode culture dish outputs electrode signals through four wells 12 every five gold fingers 11. The probe is connected to the gold fingers to transmit the electrode signals.

[0044] The existing connection structure between probes and microplates is poorly designed. Poor insulation between probes easily leads to signal crosstalk between test groups and individual probes, causing interference between cellular electrical signals at different locations and resulting in measurement deviations. Furthermore, the unreasonable design of the signal extraction interface introduces significant external interference during signal transmission, further exacerbating the interference problem, degrading signal quality, and resulting in severely distorted cellular electrical signals that cannot provide reliable data for research and applications.

[0045] Existing measurement modules have low integration and complex connections between components. When core measurement components (such as probes) malfunction, individual repair and replacement are difficult, often requiring the entire module to be replaced, making repairs extremely difficult and maintenance costs high. Furthermore, the measurement modules lack effective waterproofing and moisture-proofing, making them prone to failure in high-humidity environments, affecting the normal conduct of experiments and reducing the stability and reliability of the device. The measurement module includes a probe assembly 100, a probe mounting plate 200, and a circuit board 300.

[0046] The probes (probe head 110 and probe sleeve 120) in this embodiment are entirely made of 6061 aluminum alloy and manufactured using Computer Numerical Control (CNC) machining, with some parts employing a color-changing anodizing process, giving the device good aesthetics and structural stability. The probe mounting plate 200 is made of bakelite using CNC machining, ensuring good insulation between the test components and individual probes, reducing signal crosstalk, and ensuring the quality of the acquired signals. The overall probe structure is made of 6061 aluminum alloy, while the probe mounting plate 200 is made of bakelite, ensuring the structural stability and overall rust resistance of the equipment.

[0047] For signal extraction from cell microelectrode culture dishes, a probe scheme combining a probe head 110 and a probe sleeve 120 is employed. This allows the probe head 110 to be easily removed and replaced, improving the convenience of probe maintenance. A probe mounting plate 200, manufactured using bakelite through computer numerical control (CNC), is used. The probe sleeve 120 passes through the probe mounting plate 200 and is soldered to the circuit board 300 beneath the mounting plate, achieving a stable connection between the probe sleeve 120 (probe needle sleeve) and the circuit board 300 at both the signal and structural levels. Finally, inserting the probe head 110 (probe needle body) into the probe sleeve 120 completes the assembly of the overall measurement module, enabling measurement and signal switching functions.

[0048] This application embodiment also applies waterproof and moisture-proof treatment to the probe head 110, probe sleeve 120, and circuit board 300, making them easy to repair and replace while also possessing good stability. The waterproof and moisture-proof treatment includes conformal coating and potting compound treatment.

[0049] Thanks to the modular design of the probe assembly 100, probe mounting plate 200, and circuit board 300, the measurement module can be individually waterproofed. After being treated with a waterproof coating, the measurement module, including the probe assembly 100, probe mounting plate 200, and circuit board 300, can achieve stable operation of the measurement system in high humidity and high temperature environments. Furthermore, the waterproof and moisture-proof treatment also provides rust resistance to the probe head 110, probe sleeve 120, and circuit board 300. The use of probes separated from the probe head 110 by the probe sleeve 120 allows for convenient probe replacement. When some probe heads 110 age, the aged probe heads 110 can be individually removed and replaced with new ones, simplifying the replacement process and reducing daily maintenance costs.

[0050] In embodiments of this application, the connector 400 includes an aviation connector, such as a 9-pin aviation connector. The connector 400 may also include other types of connectors with waterproof and moisture-proof functions.

[0051] Because an aviation connector 400 is used at the signal output end to connect to the circuit board 300 and is fixed to the housing, external signal interference is reduced, ensuring the stability of signal transmission. This also guarantees the stability of the connector 400 interface in high-humidity environments, and the smaller interface size saves space. Furthermore, since the aviation connector is a universal signal interface, it can be used as a high-throughput signal measurement solution and can be integrated with different experimental equipment to achieve multiple functions and adapt to various experimental needs in the laboratory.

[0052] like Figure 5 As shown, the embodiments of this application also include: a plurality of elastic positioning posts 410 and a plurality of positioning pins 420; one end of the plurality of elastic positioning posts 410 is fixed on the circuit board 300, and the other end passes through the probe mounting plate 200; one end of the plurality of positioning pins 420 is fixed on the side of the probe mounting plate 200 away from the circuit board 300.

[0053] like Figure 5 As shown, the embodiments of this application further include: a culture dish base 500; the culture dish base 500 is placed on the other end of a plurality of elastic positioning posts 410. The culture dish base 500 is used to place the microelectrode culture dish 20.

[0054] like Figure 7 As shown, the embodiments of this application also include: a top cover 700; one long side of the top cover 700 is connected to one long side of the outer shell 600 via a connecting part 800.

[0055] The connecting part 800 includes a hinge; the top cover 700 also includes a vent hole.

[0056] like Figure 8 As shown, the embodiments of this application also include: a housing 600; a petri dish base 500, multiple probe groups 100, a probe mounting plate 200, a circuit board 300 and a connector 400 are all placed inside the housing 600.

[0057] The embodiments of this application also include: a locking structure 900; the locking structure 900 includes a locking sleeve and a sliding lock;

[0058] like Figure 7 As shown, the locking sleeve is fixed to one side of the other long side of the upper cover 700, and the sliding lock is fixed to one side of the other long side of the outer shell 600; or the locking sleeve is fixed to one side of the other long side of the outer shell 600, and the sliding lock is fixed to one side of the other long side of the upper cover 700.

[0059] The connecting part 800 includes a hinge. The locking structure 900 may also include a latch, a magnetic latch, or an electronic lock.

[0060] In terms of ease of operation, the existing solutions have complex device cover connection methods and unstable locking structures. Placing and removing culture dishes is not only cumbersome and inconvenient, consuming a significant amount of time and effort, but may also damage the equipment or culture dishes due to improper operation. Furthermore, since the probes are used to connect and fix the culture dishes, replacing the probes requires disassembling multiple parts, resulting in numerous operational steps and greatly increasing the difficulty and time cost of experimental operations.

[0061] like Figure 6 and Figure 7 As shown, the embodiment of this application uses a hinge installed on one side of the long side (long side end) of the top cover 700, and a locking sleeve or self-locking slip buckle on the other side (the other long side), forming a single-sided sliding locking structure 900 with the outer shell 600. This single-sided sliding locking method, achieved by pressing the top cover 700 together with its own weight (machined by computer numerical control), allows for easy pressing of the top cover 700. It automatically locks when pressed to the bottom, ensuring effective pressing and connection stability. After the top cover 700 is pressed, the culture dish and its base 500 are pressed downwards. Four elastic positioning posts 410 at both ends move downwards in a specified direction, ultimately connecting the gold fingers of the culture dish with the probe, effectively reducing the pressure on the culture dish (microelectrode culture dish). When disconnected, the upper cover 700 can be unlocked by opening the locking mechanism. The upper cover 700 can be opened to any position and suspended. Two positioning pins 420 on each side assist in repositioning the culture dish base 500, facilitating its removal. The locking structure 900, multiple elastic positioning posts 410, and multiple positioning pins 420 work together to facilitate the placement and removal of the culture dish, while also ensuring a stable connection between the culture dish and the probe after the cover is closed. The upper cover 700 has a vented cap, allowing for effective gas exchange and ensuring proper temperature, humidity, and carbon dioxide concentration during the culture process.

[0062] Secondly, according to the embodiments of this application, a signal acquisition device for a microelectrode culture dish is also proposed, characterized in that it includes the signal transmission device for a microelectrode culture dish as described in the first aspect, and further includes a signal measurement circuit; the signal measurement circuit is placed on the circuit board 300 and is electrically connected to the plurality of probe groups 100 and the connector 400.

[0063] The signal transmission device provided in this application is a base device capable of high-throughput 96-well configuration. It integrates a micro probe array and a measurement circuit as a signal processing module. Through a standardized probe layout (24 sets of mirrored interfaces, adaptable to culture dishes with up to 96 wells), it becomes an integrated probe array module. This enables the signal acquisition device for microelectrode culture dishes proposed in this application to achieve multi-well synchronous signal acquisition, solving the problem that existing solutions cannot achieve high-throughput stable measurement, and ensuring the high efficiency and stability of signal acquisition.

[0064] The embodiments of this application can be used for electrophysiological signal acquisition, chemical reaction monitoring, or biosensing applications in porous microplates. This signal acquisition device achieves high-precision, high-efficiency multi-channel data acquisition and analysis through a probe group 100 as an integrated microprobe array and a signal measurement circuit as a signal processing module.

[0065] In this embodiment, the circuit board is placed under the probe mounting plate, and the probe sleeve passes through the probe mounting plate, thereby fixing the probe sleeve and improving the overall stability of the probe. By dividing the probe into a probe sleeve and a probe head, one end of the probe sleeve passing through the probe mounting plate is soldered to the circuit board, and the probe head is inserted into the probe sleeve, enabling quick replacement of the probe head and improving maintenance convenience. Furthermore, this embodiment also solves key problems of existing solutions, such as difficulty in stable measurement of high-throughput signals, high signal interference, poor compatibility, cumbersome operation, and difficult maintenance. Existing technologies may have limitations in material selection; using only ordinary metal materials may not simultaneously ensure structural stability, rust resistance, and insulation, and may also result in insufficient aesthetics; using only insulating materials may lead to insufficient structural strength. This embodiment selects 6061 aluminum alloy as the overall structural material for the probe and uses CNC-machined bakelite as the probe mounting plate, ensuring structural stability and overall rust resistance; some areas use a color-changing anodizing process to improve aesthetics; the core probe mounting plate is made of bakelite and CNC-machined, ensuring good insulation. This specific material combination of 6061 aluminum alloy and bakelite perfectly solves the problem of existing technologies where materials cannot simultaneously meet multiple performance requirements, achieving multiple advantages in terms of structure, rust prevention, insulation, and aesthetics. In existing technologies, the connection between the probe and the device may be relatively fixed, requiring the disassembly of many components for probe replacement, which is complex and may affect the stability of the signal connection, leading to a decrease in measurement accuracy after replacement. The implementation of this application adopts a probe sleeve and probe head combination scheme, where a probe consists of a sleeve and a probe itself, serving as the core measurement probe module. The base for mounting the probe assembly is a probe mounting plate made of bakelite using CNC machining. The probe sleeve passes through the probe mounting plate and is soldered to the circuit board, achieving a stable dual connection between the probe sleeve and the circuit board at both the signal and structural levels. Probe assembly is completed simply by inserting the probe head into the probe sleeve, and replacement only requires removing the probe head separately, making the operation simple. This design solves the problems of difficult probe replacement and unstable signal connection after replacement in existing technologies, ensuring the stable implementation of measurement and signal switching functions. Existing measurement modules often lack specialized waterproofing and moisture-proofing treatments, making them prone to malfunction in high-humidity environments. Furthermore, their low integration makes repair and replacement inconvenient, and probe aging may necessitate complete module replacement, resulting in high maintenance costs. The embodiment of this application presents an independent measurement module that integrates a probe set, probe mounting plate, and circuit board, achieving functional integration and facilitating repair and replacement. Through impregnation with a waterproof coating, it can operate stably in high-humidity environments. The separate design of the probe sleeve and probe head allows for individual probe replacement, reducing maintenance costs. This design solves the problems of poor stability, inconvenient repair and replacement, and high maintenance costs of existing measurement modules in high-humidity environments, improving system stability and economy.Existing technologies may suffer from complex operation, insufficient locking stability, and the potential for excessive pressure on the culture dish during pressing, affecting measurement results. Furthermore, the culture dish base 500 is difficult to remove after the cover is opened. The embodiment of this application installs a hinge on one long side of the top cover and outer shell, and uses a self-locking sliding joint on the other side to form a single-sided sliding locking structure. The weight of the top cover allows for easy pressing and automatic locking, ensuring connection stability. After the top cover is pressed, four elastic positioning pins 410 connect the culture dish and probe, reducing pressure on the culture dish. After the lock is opened, the top cover can be suspended, and two positioning pins assist in the return of the culture dish base 500 to its original position, facilitating removal of the culture dish. This design solves the problems of complex cover operation, unstable locking, excessive pressure on the culture dish, and inconvenient removal of the base in existing technologies, improving operational convenience and measurement accuracy. Existing technologies may also suffer from inconsistent signal interfaces, poor versatility, difficulty in adapting to cell microelectrode culture dishes with different numbers of wells and functions, insufficient stability of the interface in high humidity environments, and large space requirements. The implementation method of this application uses a probe group to acquire signals as a whole. The standard interface consists of five probes arranged in a cross pattern with a spacing of 2.54 mm, forming a mirror interface through 24 groups. This allows it to be adapted to culture dishes with up to 96 wells and different functions, providing strong versatility. The signal output end uses a 9-pin aviation connector to connect to the circuit board and is fixed to the outer shell, ensuring stability in high humidity environments and saving space. This design solves the problems of poor interface versatility, insufficient stability, and large space occupation in the prior art, meeting various experimental needs in the laboratory.

[0066] The above description is merely a preferred embodiment of this application, but the scope of protection of this application is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the technical scope disclosed in this application should be included within the scope of protection of this application. Therefore, the scope of protection of this application should be determined by the scope of the claims.

Claims

1. A signal transmission device for microelectrode culture dishes, characterized in that, include: Multiple probe sets, probe mounting plate, circuit board, connector plug, culture dish base, top cover, multiple flexible positioning columns and multiple positioning pins; among them, the multiple flexible positioning columns include 4 flexible positioning columns; The probe mounting plate is located on one side of the circuit board; Each of the plurality of probe groups includes five probes arranged in a cross pattern, with the distance between two adjacent probes being a first interval; the first interval is 2.54 mm. Each of the probes includes a probe head and a probe sleeve; One of the probe sleeves passes through the probe mounting plate; one end of the probe sleeve extends beyond the probe mounting plate to the side facing away from the circuit board; the other end of the probe sleeve is soldered to the circuit board. The probe head is inserted into the probe sleeve; The probe group is used to connect to the signal output terminal of the microelectrode culture dish and receive the signal from the microelectrode culture dish; The connector plug is connected to the circuit board and is used to transmit the signals received by the probe group; One end of each of the plurality of elastic positioning posts is fixed to the circuit board, and the other end passes through the probe mounting plate; One end of each of the plurality of positioning pins is fixed to the side of the probe mounting plate opposite to the circuit board; The petri dish base is placed on the other end of the plurality of elastic positioning columns; After the top cover is pressed, the microelectrode culture dish and the culture dish base are pressed together and moved downwards; after the top cover is pressed, the microelectrode culture dish and the probe are connected by 4 elastic positioning columns, which reduces the pressure on the microelectrode culture dish.

2. The signal transmission device according to claim 1, characterized in that, Also includes: The outer casing; the culture dish base, multiple probe groups, probe mounting plate, circuit board and connector are all placed inside the outer casing.

3. The signal transmission device according to claim 2, characterized in that, The long side of the top cover is connected to the long side of the outer shell via a connecting part.

4. The signal transmission device according to claim 3, characterized in that, Also includes: A locking structure; the locking structure includes a locking sleeve and a sliding lock; The locking sleeve is fixed to one side of the other long side of the upper cover, and the sliding lock is fixed to one side of the other long side of the outer shell; or The locking sleeve is fixed to one side of the other long side of the outer shell, and the sliding lock is fixed to one side of the other long side of the upper cover.

5. The signal transmission device according to claim 1, characterized in that, The probe head and the probe sleeve are made of gold-plated stainless steel.

6. The signal transmission device according to claim 1, characterized in that, The probe mounting plate is made of bakelite.

7. A signal acquisition device for microelectrode culture dishes, characterized in that, The device includes a signal transmission apparatus for a microelectrode culture dish as described in any one of claims 1 to 6, and further includes a signal measurement circuit; the signal measurement circuit is disposed on the circuit board and electrically connected to the plurality of probe groups and the connector plug.

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