一种橡树耐盐QrMBF1c基因、蛋白及其应用

By cloning and validating the oak MBF1c gene (QrMBF1c), constructing a recombinant expression vector, and validating its salt tolerance in yeast and Arabidopsis thaliana, the research gap in oak salt tolerance was filled, the resources of oak salt tolerance genes were enriched, and targets for molecular breeding were provided, thus promoting the ecological restoration of saline-alkali land.

CN121160719BActive Publication Date: 2026-07-17SICHUAN AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SICHUAN AGRI UNIV
Filing Date
2025-08-28
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

The lack of key gene resources in oak salt tolerance research, and the lack of reports on the regulatory function of the MBF1c gene, have affected the development of oak salt tolerance breeding and saline-alkali land ecological restoration.

Method used

The coding sequence and protein of the oak MBF1c gene (QrMBF1c) were cloned and verified. A recombinant expression vector was constructed, and its salt tolerance was verified by transformation with yeast and Arabidopsis thaliana. Agrobacterium-transformed lines were prepared to achieve efficient expression of the gene in plants.

Benefits of technology

This study enriched the salt tolerance gene resources of oak trees, provided target genes for molecular breeding, expanded the suitable habitat area of ​​oak trees, promoted the greening and ecological restoration of saline-alkali land, and revealed the salt tolerance regulation mechanism of QrMBF1c.

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Abstract

本发明公开了一种橡树耐盐QrMBF1c基因、蛋白及其应用,属于生物技术领域,本发明从夏栎中分离并克隆了转录共激活因子QrMBF1c基因编码序列全长,并利用酵母和拟南芥过表达分析证实了QrMBF1c对盐胁迫的耐受能力,明确了QrMBF1c是橡树耐盐性分子育种的重要基因资源,具备良好的应用前景。本发明填补了MBF1c基因在橡树耐盐性研究领域的空白,拓展了MBF1c基因在木本植物盐胁迫应答中的功能认知。同时,通过该基因的应用,有望加速橡树耐盐新品种的培育进程,助力盐碱地生态修复与木本植物产业的良好发展。
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Citation Information

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