A soothing repair plant extract complex, and methods of making and using the same

Abstract

The application belongs to the field of cosmetics and provides a soothing and repairing plant extract composition, which is prepared from cactus extract, oat bran extract, radix ophiopogonis extract, radix paeoniae extract, radix scrophulariae extract, butanediol and water. The preparation method of the soothing and repairing plant extract composition comprises the following steps: (1) cactus extract is configured into an aqueous solution, and radix ophiopogonis extract is configured into an aqueous solution; the two aqueous solutions are mixed at a volume ratio of 1:1; reaction is carried out in a water bath at 85-90 DEG C for 10-12 hours; the reaction product is concentrated and then freeze-dried; (2) the product of step (1), oat bran extract, radix paeoniae extract, radix scrophulariae extract, butanedioli are mixed and subjected to high-pressure homogenization; (3) water is added to the product of step (2), which is subjected to high-pressure homogenization again and then filtered. The composition has good soothing and repairing effects and can be used in various cosmetics.

Description

Technical Field

[0001] This application belongs to the field of cosmetics. Specifically, this application provides a soothing and repairing plant extract complex, its preparation method, and its application. Background Technology

[0002] Skin allergic reactions refer to abnormal, specific immune responses that occur when the body is stimulated by certain antigens, primarily characterized by physiological dysfunction or tissue and cell damage. Common symptoms include redness, swelling, itching, dryness, and peeling, affecting appearance and significantly impacting daily life. Environmental factors, lifestyle and work rhythms, and the influence of daily chemical products contribute to very common skin allergies, making them a widespread concern in the fields of dermatology treatment and cosmetics.

[0003] Compared to histamine-based anti-allergens, plant extracts have certain advantages in terms of the diversity of anti-allergy mechanisms, safety, and other functions such as providing barrier repair. Therefore, developing soothing and repairing cosmetic compositions using plant extracts is an effective way to solve the above problems.

[0004] The applicant previously determined a compositional formula including cactus extract, oat bran extract, ophiopogon root extract, peony root extract, and scutellaria root extract, and applied for and obtained the relevant patent CN 110200830 B. Summary of the Invention

[0005] To further improve the effects of plant extract compositions, this application provides a soothing and repairing plant extract complex prepared using raw materials including cactus extract, oat bran extract, ophiopogon root extract, peony root extract, scutellaria root extract, butylene glycol, and water.

[0006] Furthermore, the raw materials for preparing the soothing and repairing plant extract complex are: 5-10 wt% cactus extract, 2-5 wt% oat bran extract, 2-5 wt% ophiopogon root extract, 1-3 wt% peony root extract, 0.5-2 wt% scutellaria root extract, 45-55 wt% butylene glycol, and the balance being water.

[0007] Furthermore, the raw materials for preparing the soothing and repairing plant extract complex are: 7wt% cactus extract, 3wt% oat bran extract, 3wt% ophiopogon root extract, 2wt% peony root extract, 1wt% scutellaria root extract, 50wt% butylene glycol, and 34wt% water.

[0008] Furthermore, the cactus extract contains ≥68wt% polysaccharide; the oat bran extract contains ≥85wt% oat alkaloids; the ophiopogon root extract contains ≥70wt% polysaccharide; the peony root extract contains ≥60wt% paeoniflorin, 4-5wt% paeonol, and 7-9wt% catechins; and the scutellaria root extract contains ≥85wt% baicalin.

[0009] Furthermore, the preparation method of the soothing and repairing plant extract complex includes:

[0010] Step (1): Prepare an 8-12 w / v% aqueous solution of cactus extract and an 8-12 w / v% aqueous solution of Ophiopogon japonicus root extract; mix the two aqueous solutions in a 1:1 volume ratio; react in a water bath at 85-90℃ for 10-12 hours; concentrate the reactants and freeze dry.

[0011] Step (2): Thoroughly mix the product from step (1), oat bran extract, peony root extract, scutellaria root extract, and butylene glycol, and homogenize under high pressure;

[0012] Step (3): Add water to the product from step (2), homogenize again under high pressure, and filter to obtain the composition.

[0013] Further, step (1) involves preparing a 10 w / v% aqueous solution of cactus extract and a 10 w / v% aqueous solution of Ophiopogon japonicus root extract; mixing the two aqueous solutions in a 1:1 volume ratio; reacting in a 90°C water bath for 10 hours; and then lyophilizing the reactants after concentration.

[0014] Furthermore, the high-pressure homogenization pressure in step (2) is 1000-1200 bar; the high-pressure homogenization pressure in step (3) is 1300-1500 bar.

[0015] Furthermore, the high-pressure homogenization pressure in step (2) is 1200 bar; the high-pressure homogenization pressure in step (3) is 1500 bar.

[0016] On the other hand, this application provides the application of the above-mentioned soothing and repairing plant extract complex in the preparation of cosmetics.

[0017] Furthermore, the amount of the soothing and repairing plant extract complex added to the cosmetic is 0.1wt%-10wt%.

[0018] Furthermore, the amount of the soothing and repairing plant extract complex added to the cosmetic is 0.5wt%-5wt%.

[0019] The cosmetics include, but are not limited to, facial masks, moisturizing creams, facial cleansers, toners, lotions, sunscreens, BB creams, shampoos, shower gels, and skin gels. Those skilled in the art can adjust the preparation methods of the cosmetics appropriately according to the properties of the raw materials and product requirements.

[0020] On the other hand, this application also provides a method for preparing a soothing and repairing plant extract complex; the raw materials for preparing the plant extract composition are: 7wt% cactus extract, 3wt% oat bran extract, 3wt% ophiopogon root extract, 2wt% peony root extract, 1wt% scutellaria root extract, 50wt% butylene glycol, and 34wt% water; the method for preparing the plant extract composition includes:

[0021] Step (1): Prepare a 10 w / v aqueous solution of cactus extract and a 10 w / v aqueous solution of Ophiopogon japonicus root extract; mix the two aqueous solutions in a 1:1 volume ratio; react in a 90℃ water bath for 10 hours; concentrate the reactants and freeze dry.

[0022] Step (2): Thoroughly mix the product from step (1), oat bran extract, peony root extract, scutellaria root extract, and butylene glycol, and homogenize under high pressure;

[0023] Step (3): Add water to the product from step (2), homogenize again under high pressure, and filter to obtain the composition.

[0024] By performing Maillard reactions on two crude aqueous extracts containing high levels of protein, this application further improves the soothing effect based on previous compositions, providing a good foundation for further improvements in the effects of the product and the corresponding cosmetic. Attached Figure Description

[0025] Figure 1 The results of hyaluronidase inhibition experiments for various compositions are shown.

[0026] Figure 2 Results of behavioral easing experiments on zebrafish embryos using various compositions.

[0027] Figure 3 The results of hyaluronidase inhibition experiments on products at different temperatures are shown.

[0028] Figure 4 The results of hyaluronidase inhibition experiments for products at different reaction times are shown.

[0029] Figure 5 Results of behavioral easing experiments on zebrafish embryos with products of different reaction times. Detailed Implementation

[0030] Example 1: Basic Formulation and Method

[0031] The basic formula is:

[0032] Cactus extract 7wt%, oat bran extract 3wt%, ophiopogon root extract 3wt%, peony root extract 2wt%, scutellaria root extract 1wt%, butylene glycol 50wt%, water 34wt%.

[0033] Among the above ingredients, the cactus extract used is a water extract of prickly pear cactus with a polysaccharide content ≥68wt%; the oat bran extract has an oat alkaloid content ≥85wt%; the ophiopogon root extract is a water extract of ophiopogon root with a polysaccharide content ≥70wt%; the peony root extract has a paeoniflorin content ≥60wt%, a paeonol content of 4-5wt%, and a catechin content of 7-9wt%; and the scutellaria root extract has a baicalin content ≥85wt%.

[0034] Basic preparation method:

[0035] The cactus extract, oat bran extract, ophiopogon root extract, peony root extract, scutellaria root extract, and butylene glycol were thoroughly mixed and added to a high-pressure microfluidic homogenizer for homogenization at an operating pressure of 1200 bar. Water was added to the homogenized product, and the mixture was added to the high-pressure microfluidic homogenizer again for homogenization at an operating pressure of 1500 bar. The mixture was then filtered to obtain the final composition.

[0036] In vitro inhibition assay of hyaluronidase:

[0037] The test samples were divided into four groups: A, B, C, and D. 0.5 mL of hyaluronidase (500 U / mL) was added to groups A and C, and 0.5 mL of acetate buffer (pH 5.6) was added to groups B and D. Then, 0.1 mL of CaCl2 solution (2.5 mol / L) was added to each group and the samples were kept in a constant temperature water bath at 37°C for 20 min.

[0038] Take out group A and group B and add sample solution (5mg / mL) respectively, and add distilled water to group C and group D respectively. Then keep warm in a constant temperature water bath at 37℃ for 20min.

[0039] Take out group A and group C and add 0.5 mL of sodium hyaluronate solution (0.5 mg / mL), and add 0.5 mL of acetic acid solution (pH 5.6) to group B and group D respectively. Then keep warm in a 37℃ constant temperature water bath for 30 min.

[0040] Take out groups A through D and place them at room temperature for 5 minutes. Then add 0.5 mL of NaOH solution (0.5 mol / L) and 0.5 mL of acetylacetone solution (3.5 mL acetylacetone + 50 mL 1.0 mol / L sodium carbonate solution) to each group. Then boil in a water bath for 15 minutes, in an ice water bath for 5 minutes, and place at room temperature for 10 minutes. Add 1 mL of Ehrlich's reagent (0.8 g p-dimethylaminobenzaldehyde + 15 mL concentrated hydrochloric acid + 15 mL anhydrous ethanol) to each group, shake thoroughly, and then add 3 mL of anhydrous ethanol to dilute. Place at room temperature for 20 minutes for color development and measure the absorbance value. Calculate the hyaluronidase inhibition rate = [(CD) - (AB)] / (CD) × 100%.

[0041] Zebrafish embryo behavioral relaxation experiment:

[0042] Before the experiment, AB line zebrafish embryos were prepared 36 hours after fertilization; 5 embryos were added to each well of the plate and washed twice with distilled water.

[0043] Add 100 μL of water to each well in the blank group and the model group, and add 100 μL of 2% w / v test solution to each well in the sample group (5 parallel experiments). Cover the culture plate with the panel and incubate at 28°C for 1 hour.

[0044] Add 100 μL of 500 μM sodium dodecyl sulfate solution to each well, incubate at 28°C for 15 minutes, and then start recording a 30-second video.

[0045] The number of spin movements of each embryo in the observation video was counted, and the relative stimulation intensity was calculated as: number of spin movements of embryos in the sample group or model group / number of spin movements of embryos in the blank group.

[0046] Example 2: Treatment of cactus extract and Ophiopogon japonicus root extract using Maillard reaction

[0047] The oat bran extract and scutellaria root extract used in this application are oat alkaloids and baicalin of a certain purity; the peony root extract has also been purified and mainly contains paeoniflorin, paeonol, and catechins. The cactus extract and ophiopogon root extract used are water extracts, which, in addition to polysaccharides as the main component, also contain a certain amount of protein (determined by the Coomassie brilliant blue method, using bovine serum albumin as the standard; the protein content in the cactus extract is 16-18 wt%, and the protein content in the ophiopogon root extract is 13-14 wt%). Currently, there is very little research on the properties of these proteins.

[0048] The applicant attempted to improve the properties of cactus extract and ophiopogon root extract by performing Maillard reactions:

[0049] Reactant 1:

[0050] A 10% aqueous solution of cactus extract and a 10% aqueous solution of Ophiopogon japonicus root extract were prepared. The two aqueous solutions were mixed in a 1:1 volume ratio. The mixture was reacted in a water bath at 85°C for 8 hours. The reactants were concentrated and freeze-dried to obtain a light brown powder.

[0051] Reactant 2:

[0052] The cactus extract was prepared into a 10% aqueous solution; the reaction was carried out in a water bath at 85°C for 8 hours; the reactants were concentrated and freeze-dried to obtain a light brown powder.

[0053] Reactant 3:

[0054] The root extract of Ophiopogon japonicus was prepared into a 10% aqueous solution; the two aqueous solutions were mixed in a volume ratio of 1:1; the mixture was reacted in a water bath at 85°C for 8 hours; the reactants were concentrated and freeze-dried to obtain a light brown powder.

[0055] Compositions 1-3 were prepared by replacing the cactus extract and ophiopogon root extract in the preparation method of Example 1 with reactants 1-3.

[0056] Example 3: Soothing effect of the improved composition

[0057] The results of capsaicin skin irritation test and carrageenan inflammation test showed that compositions 1-3 of Example 2 had similar soothing and repairing effects to the compositions of Example 1 and the compositions of Example 1 of this application and CN110200830B.

[0058] In the areas of hyaluronidase inhibition experiments and zebrafish embryo behavioral relaxation experiments, such as Figure 1 and Figure 2 As shown, mixing cactus extract and Ophiopogon japonicus root extract and performing Maillard reaction effectively improved the hyaluronidase inhibition rate and the soothing effect on zebrafish embryos. However, performing Maillard reaction on cactus extract and Ophiopogon japonicus root extract alone did not have this effect. Composition 3 (Maillard reaction of Ophiopogon japonicus root extract alone) even had the opposite effect on the hyaluronic acid inhibition rate.

[0059] Composition 1 was selected for further investigation of Maillard reaction parameters.

[0060] Example 4: Study of Maillard Reaction Parameters

[0061] Maintaining a constant reaction time of 8 hours, the effects of the Maillard reaction were compared at 85, 90, 100, and 120°C (120°C was achieved using an oil bath). The soothing effect on zebrafish embryos was generally consistent across the different temperatures. Figure 3As shown, the reaction at 120℃ has a detrimental effect on the inhibition of hyaluronidase, and the product at 120℃ is also darker in color, which is not conducive to the appearance quality of cosmetic products. Therefore, 90℃, which has an inhibitory effect on hyaluronidase and is at a lower temperature, was chosen.

[0062] The effects of reactants on different reaction times were studied at 90℃, and the results are as follows: Figure 4 , 5 As shown, a reaction time of 16 hours not only intensified the product color but also had adverse effects on both of the soothing experiments of interest. Given that the effects of 10 hours and 12 hours were not significantly different, 10 hours was preferred for both color accuracy and time / energy savings.

Claims

1. A soothing and repairing plant extract complex, characterized in that, The raw materials for preparing the soothing and repairing plant extract complex are: 7wt% cactus extract, 3wt% oat bran extract, 3wt% ophiopogon japonicus root extract, 2wt% peony root extract, 1wt% scutellaria baicalensis root extract, 50wt% butylene glycol, and 34wt% water; the cactus extract is a water extract of cactus, wherein the polysaccharide content is ≥68wt% and the protein content is 16-18wt%. The oat bran extract contains ≥85wt% oat alkaloids; the Ophiopogon japonicus root extract is an aqueous extract of Ophiopogon japonicus root, containing ≥70wt% polysaccharides and 13-14wt% protein; the Paeonia lactiflora root extract contains ≥60wt% paeoniflorin, 4-5wt% paeonol, and 7-9wt% catechins; the Scutellaria baicalensis root extract contains ≥85wt% baicalin. The preparation method of the soothing and repairing plant extract complex includes: Step (1): Prepare a 10 w / v aqueous solution of cactus extract and a 10 w / v aqueous solution of Ophiopogon japonicus root extract; mix the two aqueous solutions in a 1:1 volume ratio; react in a 90℃ water bath for 10 hours; concentrate the reactants and freeze dry. Step (2): Mix the product from step (1), oat bran extract, peony root extract, scutellaria root extract, and butylene glycol, and homogenize under high pressure; the high pressure homogenization pressure is 1000-1200 bar. Step (3): Add water to the product of step (2) and homogenize under high pressure; filter to obtain the soothing and repairing plant extract complex; the high pressure homogenization pressure is 1300-1500 bar.

2. According to claim 1, the high-pressure homogenization pressure in step (2) of the preparation method of the soothing and repairing plant extract complex is 1200 bar; and the high-pressure homogenization pressure in step (3) is 1500 bar.

3. The application of the soothing and repairing plant extract complex according to claim 1 or 2 in the preparation of cosmetics, wherein the amount of the soothing and repairing plant extract complex added to the cosmetic is 0.5wt%-5wt%.

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