Microbial complex culture device and method thereof

Abstract

The application provides a microbial compound culture device and a method thereof, and belongs to the technical field of microbial culture. The device comprises a frame body, an algae pool, a microbial culture pool and a zooplankton culture pool are sequentially arranged in the frame body from inside to outside, a center cylinder is arranged in the middle of the algae pool, an installation partition plate is arranged in the lower end of the center cylinder, a water delivery device is arranged in the middle of the installation partition plate, the water pumping end of the water delivery device is located below, the water draining end is located above the inside of the algae pool, the algae pool and the microbial culture pool are separated by a first partition plate, the upper end of the first partition plate is provided with a sunlight shed, the microbial culture pool and the zooplankton culture pool are separated by a second partition plate, and a carrier frame is arranged in the inside of the microbial culture pool. The algae pool, the microbial culture pool and the zooplankton culture pool are arranged in the frame body, so that the algae, the bacterial microorganism, the formicidae and the zooplankton can be jointly cultured, and a compound microbial culture system is constructed.

Description

Technical Field

[0001] This invention belongs to the field of microbial culture technology, specifically a microbial composite culture device and method. Background Technology

[0002] Microbial communities are structural and functional units that are widely present in ecosystems. They are a vibrant part of the ecosystem, and different populations in microbial communities can coexist in a regular way. At the same time, they have their own distinct nutritional and metabolic types. In a sense, the development of microbial communities has led to the development of life. With the gradual deepening of modern biology, people have been able to verify many biological questions through microbial culture.

[0003] Some microorganisms can be cultivated using culture devices. Microbial culture devices are widely used in water environment management and aquaculture. In these fields, microbial culture devices are generally set up independently of the water environment and mainly exist in the form of tanks. The cultivation conditions are harsh and require a certain amount of land area. The cultivated microorganisms need to be transferred, stored or put into use, which is uneconomical.

[0004] Therefore, we propose a microbial co-culture device and method to solve the problems encountered above. Summary of the Invention

[0005] The purpose of this invention is to address the problems that microbial culture devices are widely used in water environment management and aquaculture, but in these fields, they are generally set up independently of the water environment, mainly in the form of tanks, with harsh cultivation conditions, requiring a certain amount of land area, and the cultivated microorganisms need to be transferred, stored or put into use, which is uneconomical. Therefore, this invention proposes a microbial composite culture device and method.

[0006] The objective of this invention can be achieved through the following technical solution: A frame is included, inside which, from the inside out, are arranged an algae pond, a microbial culture pond, and an algae-eating insect culture pond. A central cylinder is located in the middle of the algae pond, and a mounting partition is located inside the lower end of the central cylinder. A water conveying device is located in the middle of the mounting partition, with the water pumping end located below and the water draining end located inside the algae pond above. A filtration mechanism with a filtration pore size of 0.01-200 μm is located inside the water pumping end. The algae pond and the microbial culture pond are connected by a partition. A partition is provided at the top of the partition, which is located above the algae pond. The microbial culture pond and the algae-eating insect culture pond are separated by a partition. The microbial culture pond is provided with a carrier frame inside, and a transmission mechanism is provided on one side of the carrier frame. The bottom of the frame is provided with filler. There is a certain space between the lower end of the partition and the upper end of the filler, so that the algae pond and the microbial culture pond are connected through the partition and the filler. A submerged plant planting area is provided above the filler inside the algae-eating insect culture pond.

[0007] In a preferred embodiment of the present invention, the carrier frame is rotatably installed inside the microbial culture tank via a rotating shaft. The left and right ends of the rotating shaft are rotatably connected to partition one and partition two, respectively. The interior of the carrier frame is respectively provided with a wasp culture area, a microbial culture area and a seaweed culture area, and each of the wasp culture area, the microbial culture area and the seaweed culture area is provided with a carrier that matches its culture environment.

[0008] In a preferred embodiment of the present invention, the transmission mechanism includes a hollow mounting plate and a ratchet. The ratchet is disposed on the circumferential surface of the end of the rotating shaft. The hollow mounting plate is disposed on the side of the partition located in the microbial culture tank. A float is disposed inside the upper end of the hollow mounting plate, and a lifting rod is disposed at the lower end of the float. A lever is hinged to the lower end of the lifting rod, and a connecting plate is hinged to the other end of the lever via a hinge shaft. A toothed plate is disposed at the upper end of the connecting plate, and a spur gear is meshed with the side of the upper end of the toothed plate. A toggle rod is disposed inside the spur gear, and an active pawl is fixedly connected to the end of the toggle rod via a protrusion. The end of the active pawl abuts against the teeth of the ratchet. A check pawl abuts against the upper end of the ratchet, and the check pawl is rotatably mounted on the surface of the partition via a rotating rod. A return spring is disposed on the circumferential surface of the rotating rod.

[0009] In a preferred embodiment of the present invention, a limiting groove is provided on the inner side wall of the hollow mounting plate, and a limiting block is slidably installed inside the limiting groove, the limiting block being fixedly connected to the lifting rod.

[0010] In a preferred embodiment of the present invention, the lever is rotatably mounted on the surface of the partition plate by means of a fulcrum, and the fulcrum is closer to the connection point between the lifting rod and the lever. The connecting plate has a movable groove inside, and the end of the hinge shaft is inserted into the movable groove.

[0011] In a preferred embodiment of the present invention, a positioning groove is formed on the surface of the partition plate, and a positioning block is slidably installed inside the positioning groove. The outer end of the positioning block is fixedly connected to the toothed plate. An annular groove is formed on the surface of the partition plate, and a positioning rod is movably installed inside the annular groove. The outer end of the positioning rod is fixedly connected to the surface of the actuating rod. The actuating rod is sleeved on the surface of the rotating shaft, and the center of the actuating rod is the same as the center of the rotating shaft. A limiting mounting plate is provided on the surface of the partition plate, and an arc-shaped groove is formed inside the limiting mounting plate. The inner end of the protrusion is movably inserted into the arc-shaped groove.

[0012] In a preferred embodiment of the present invention, the partition plate 2 is provided with an overflow port, and the overflow port is designed in a Z-shape. The lower end of the overflow port is the liquid inlet and is located inside the microbial culture tank, and the upper end of the overflow port is the liquid outlet and is located inside the algae-eating insect culture tank. A baffle is provided next to the liquid inlet of the overflow port.

[0013] In a preferred embodiment of the present invention, a discharge port is provided on the rear side of the frame, and one is provided at each algae pond, microbial culture pond and algae-eating insect culture pond. The liquid outlet of the overflow port is located above the discharge port. A sealing plate is provided inside the discharge port. An electric telescopic rod is provided at the upper end of the sealing plate, and the electric telescopic rod is located inside the frame.

[0014] As a preferred embodiment of the present invention, a method for microbial co-culture specifically includes the following steps:

[0015] Step 1: Place the pre-cultured algae into the algae pond, and place various microbial culture carriers inside the carrier frame to simulate the microbial living and reproduction environment. Plant submerged plants at the bottom of the algae-eating insect culture pond to provide dissolved oxygen for the water and a suitable growth environment for the algae-eating insects. Then place the entire frame in the surface water environment or aquaculture pond without occupying land area.

[0016] Step 2: During the growth and reproduction of algae and the growth of submerged plants in the algae pond, the water level in the algae pond and the algae-eating insect cultivation pond will drop. At this time, the water conveying device will draw water from the outside from the bottom of the central cylinder to replenish the algae pond. When replenishing water, the water level in the central cylinder will rise and overflow the top of the central cylinder, so that the water will carry algae to the area between the partition and the central cylinder. Then the water level here will rise, and the water flow carrying algae will flow from the bottom of the partition into the microbial cultivation pond, replenishing the water and providing algae for the microbial cultivation pond. The algae can serve as food for organisms such as wasps and promote the reproduction of microorganisms.

[0017] Step 3: The water level in the microbial culture tank drops due to environmental evaporation and microbial growth. Then, water is added to raise the water level. The buoyancy of the water drives the transmission mechanism, which in turn drives the carrier frame to rotate periodically. This periodic exposure to air significantly increases the oxygen acquisition efficiency of aerobic microorganisms and helps to wash away the aging biofilm on the carrier surface, maintaining high activity.

[0018] Step 4: Water in the microbial culture tank can be discharged into the algae-eating insect culture tank through the overflow outlet to replenish the water in the algae-eating insect culture tank, prevent the submerged plants from lacking water and affecting the provision of dissolved oxygen to the water body by the submerged plants, and provide a suitable growth environment for the algae-eating insects.

[0019] Step 5: When microorganisms are needed for water purification in the external aquatic environment, the corresponding microorganisms and algae-eating insects can be selectively released into the environment by opening the sealing plate at the corresponding discharge outlet. The cultivated algae, wasps, and algae-eating insects can also be used as feed for fish and shrimp, realizing ecological aquaculture.

[0020] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0021] (1) By distributing algae ponds, microbial culture ponds and algae-eating insect culture ponds within the frame, algae, bacteria, microorganisms, wasps, and algae-eating insects can be cultured together, thus constructing a composite microbial culture system.

[0022] (2) When in use, the entire device can be placed in surface water environment or aquaculture ponds without occupying additional land area;

[0023] (3) The microorganisms cultivated in the device have the function of water purification. Each unit can be set to selectively release microorganisms needed by the water environment into the water environment. For example, in aquaculture ponds, algae, wasps, and algae-eating insects can be used as feed for fish and shrimp to achieve ecological aquaculture.

[0024] (4) By setting a transmission mechanism on the carrier frame, the transmission mechanism can drive the carrier frame to rotate periodically during the reciprocating water loss and replenishment process of the microbial culture tank, which significantly increases the oxygen acquisition efficiency of aerobic microorganisms. At the same time, it helps to wash away the aging biofilm on the carrier surface, maintain high activity, and improve the cultivation effect of microorganisms.

[0025] (5) The Z-shaped design of the overflow outlet makes the liquid inlet much lower than the microbial living area. The water flow speed generated by the liquid level difference alone cannot carry the microorganisms at the water surface into the algae-eating insect culture tank. This prevents the microorganisms from entering the algae-eating insect culture tank with the water flow when the water in the microbial culture tank enters the algae-eating insect culture tank, thus avoiding the presence of additional aquatic plants in the algae-eating insect culture tank that would affect the cultivation of algae-eating insects. Attached Figure Description

[0026] To facilitate understanding by those skilled in the art, the present invention will be further described below with reference to the accompanying drawings.

[0027] Figure 1 This is a three-dimensional structural diagram of the present invention;

[0028] Figure 2 This is a front sectional view of the present invention;

[0029] Figure 3 This is a left sectional view of the present invention;

[0030] Figure 4 This is a cross-sectional perspective view of the carrier frame of the present invention;

[0031] Figure 5 This is a partial sectional perspective view of the transmission mechanism of the present invention;

[0032] Figure 6 This is a partial perspective view of the partition plate of the present invention.

[0033] In the diagram: 1. Frame; 2. Algae pond; 3. Microbial culture pond; 4. Algae-eating insect culture pond; 5. Partition 1; 501. Positioning groove; 502. Annular groove; 6. Partition 2; 7. Carrier frame; 701. Wasp culture area; 702. Microbial culture area; 703. Ulva culture area; 8. Transmission mechanism; 801. Hollow mounting plate; 802. Ratchet; 803. Float; 804. Lifting rod; 805. Lever; 806. Fulcrum; 807. Hinge; 808. Connecting plate; 809. Movable groove; 810. Gear plate; 811. Spur gear 812. Actuating lever; 813. Active pawl; 814. Check pawl; 815. Rotating rod; 816. Return spring; 817. Limiting block; 818. Limiting groove; 819. Positioning block; 820. Positioning rod; 821. Protrusion; 822. Limiting mounting plate; 823. Arc groove; 9. Central cylinder; 10. Mounting partition; 11. Water conveying device; 12. Sunshade; 13. Flow outlet; 14. Baffle; 15. Discharge outlet; 16. Sealing plate; 17. Electric telescopic rod; 18. Packing material; 19. Submerged plant planting area; 20. Rotating shaft. Detailed Implementation

[0034] The technical solution of the present invention will be clearly and completely described below with reference to the embodiments. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0035] Example 1:

[0036] Please see Figure 1 - Figure 6As shown, a microbial composite culture device includes a frame 1. Inside the frame 1, from the inside out, are arranged an algae pond 2, a microbial culture pond 3, and an algae-eating insect culture pond 4. The algae pond 2 is located in the center of the frame 1. The two microbial culture ponds 3 are respectively located on the left and right sides of the algae pond 2. The two algae-eating insect culture ponds 4 are respectively located on the outer sides of the two microbial culture ponds 3. A central cylinder 9 is located in the middle of the algae pond 2. A partition 10 is installed inside the lower end of the central cylinder 9. A water conveying device 11 is located in the middle of the partition 10, with the water pumping end of the water conveying device 11 located at the bottom and the water draining end located at the bottom. Located inside the upper algae pond 2, the water pump end is equipped with a filtration mechanism with a pore size of 0.01-200μm. This filtration mechanism blocks impurities from the external water during pumping, preventing them from entering the cultivation pond and contaminating the water. The water conveying device 11 can be a water pump or similar device capable of pumping water from the outside into the algae pond 2. The algae pond 2 and the microbial cultivation pond 3 are separated by a partition 5. A sunroom 12 is installed at the upper end of the partition 5, positioned above the algae pond 2. The microbial cultivation pond 3 and the algae-eating insect cultivation pond 4 are separated by a partition 6. The microbial culture tank 3 is equipped with a carrier frame 7, which is rotatably installed inside the microbial culture tank 3 via a rotating shaft 20. The left and right ends of the rotating shaft 20 are rotatably connected to partition 5 and partition 6, respectively. The carrier frame 7 contains a wasp culture area 701, a microbial culture area 702, and a seaweed culture area 703. Each of the wasp culture area 701, microbial culture area 702, and seaweed culture area 703 contains a carrier that matches its culture environment. The carrier in the wasp culture area 701 can be a PVC tube bundle or a pile of shells to simulate a natural benthic environment. The environment provides hiding space. The carrier of the microbial culture area 702 can be made of porous ceramic or polyethylene fiber to maximize the attachment area of ​​microorganisms. The carrier of the seaweed culture area 703 can be made of coarse fiber woven net to facilitate the attachment and growth of seaweed. A transmission mechanism 8 is set on one side of the carrier frame 7. The inner bottom of the frame 1 is filled with filler 18. The lower end of the partition 5 and the upper end of the filler 18 have a certain space, so that the algae pond 2 and the microbial culture pond 3 are connected through the partition 5 and the filler 18. A submerged plant planting area 19 is set above the filler 18 inside the algae-eating insect culture pond 4.

[0037] It should be noted that the algae pond 2 and the microbial culture pond 3 are separated by a partition 5. However, the lower end of the partition 5 does not completely isolate the algae pond 2 and the microbial culture pond 3, so that the water levels in the algae pond 2 and the microbial culture pond 3 are the same. When water is added to the algae pond 2 to raise the water level, a water flow will be generated inside the algae pond 2 and flow towards the microbial culture pond 3. At the same time, the flowing water can also carry some algae into the microbial culture pond 3, providing food for the microorganisms and replenishing water and providing nutrients for the microbial culture pond 3.

[0038] The transmission mechanism 8 includes a hollow mounting plate 801 and a ratchet 802. The ratchet 802 is located on the circumferential surface of the end of the rotating shaft 20, and can drive the rotating shaft 20 to rotate when the ratchet 802 rotates. The hollow mounting plate 801 is located on the side of the partition 5 located in the microbial culture tank 3. A float 803 is provided inside the upper end of the hollow mounting plate 801. A lifting rod 804 is provided at the lower end of the float 803. A lever 805 is hinged to the lower end of the lifting rod 804. The other end of the lever 805 is hinged to a connecting plate 808 through a hinge pin 807. A toothed plate 810 is provided at the upper end of the connecting plate 808. The side of the upper end of the toothed plate 810 is engaged with the connecting plate. There is a spur gear 811, and a toggle lever 812 is provided inside the spur gear 811. The end of the toggle lever 812 is fixedly connected to the active pawl 813 through the protrusion 821. The end of the active pawl 813 abuts against the teeth of the ratchet 802. The upper end of the ratchet 802 abuts against the check pawl 814, and the check pawl 814 is rotatably mounted on the surface of the partition 5 through the rotating rod 815. A return spring 816 is provided on the circumferential surface of the rotating rod 815. The arrangement of the check pawl 814 and the return spring 816 ensures that when the toothed plate 810 drives the toggle lever 812 and the active pawl 813 to rotate back and forth, the ratchet 802 always rotates in one direction.

[0039] The partition 2 6 has an outlet 13 inside, which is designed in a Z-shape. The lower end of the outlet 13 is the liquid inlet located inside the microbial culture tank 3, and the upper end of the outlet 13 is the liquid outlet located inside the algae-eating insect culture tank 4. The algae-eating insect culture tank 4 contains more submerged plants, which consume more water. At the same time, because the algae-eating insect culture tank 4 is completely exposed to the outside, the water evaporates faster. Therefore, the water consumption rate inside the algae-eating insect culture tank 4 is greater than that in the microbial culture tank 3, so that the outlet 13 always supplies water from the microbial culture tank 3 to the algae-eating insect culture tank 4. A baffle 14 is set next to the liquid inlet of the outlet 13. The baffle 14 can block the water flow from the algae tank 2 into the microbial culture tank 3, preventing the water flow from directly carrying algae into the outlet 13 and causing the water flow to carry algae into the algae-eating insect culture tank 4, which would affect the cultivation of algae-eating insects.

[0040] The rear side of the frame 1 is provided with a discharge port 15, and one is provided at each algae pond 2, microbial culture pond 3 and algae-eating insect culture pond 4. The liquid outlet of the overflow port 13 is located above the discharge port 15. The discharge port 15 is provided with a sealing plate 16 inside. An electric telescopic rod 17 is provided at the upper end of the sealing plate 16 and is located inside the frame 1. The discharge port 15 located at the algae-eating insect culture pond 4 is located at a lower position inside the rear side of the frame 1, because the algae-eating insects will attach to the submerged plants. The other discharge ports 15 located at the microbial culture pond 3 and algae pond 2 are located at a lower position at the initial water level, because the microorganisms and algae float on the water surface, so that when the discharge port 15 is opened, the corresponding organisms can be smoothly discharged to the outside.

[0041] It should be noted that the initial water level inside the frame 1 only covers the lower half of the carrier frame 7 and is slightly higher than the upper end of the overflow port 13, providing a breeding space for the microorganisms inside the carrier frame 7 and allowing the water in the microbial culture tank 3 to be smoothly discharged into the algae-eating insect culture tank 4 through the overflow port 13.

[0042] Preferably, a limiting groove 818 is provided on the inner side wall of the hollow mounting plate 801, and a limiting block 817 is slidably installed inside the limiting groove 818. The limiting block 817 is fixedly connected to the lifting rod 804. The setting of the limiting groove 818 and the limiting block 817 can limit the lifting rod 804, so that when the float 803 floats up and down, the lifting rod 804 can move vertically stably.

[0043] Preferably, lever 805 is rotatably mounted on the surface of partition 5 via fulcrum 806, and fulcrum 806 is closer to the connection point between lifting rod 804 and lever 805, so that when lifting rod 804 drives one end of lever 805 to move, the other end of lever 805 moves a greater distance. Connecting plate 808 has a movable groove 809 inside, and the end of hinge shaft 807 is inserted into the movable groove 809, providing movable space for hinge shaft 807. Because lever 805 is rotatable, when the end connected to hinge shaft 807 rotates, hinge shaft 807 will move inside movable groove 809, thereby enabling lever 805 to smoothly drive toothed plate 810 to move vertically.

[0044] Preferably, the surface of partition 5 is provided with a positioning groove 501, and a positioning block 819 is slidably installed inside the positioning groove 501. The outer end of the positioning block 819 is fixedly connected to the toothed plate 810. The positioning groove 501 and the positioning block 819 provide support and limit the toothed plate 810, improving the stability of the vertical movement of the toothed plate 810. The surface of partition 5 is provided with an annular groove 502, and a positioning rod 820 is movably installed inside the annular groove 502. The outer end of the positioning rod 820 is fixedly connected to the surface of the actuating rod 812. The annular groove 502 and the positioning rod 820 allow the spur gear 811 and the actuating rod 812 to be rotatably mounted on the partition. On the surface of partition 5, a lever 812 is sleeved on the surface of the rotating shaft 20. The center of the lever 812 is the same as the center of the rotating shaft 20, so that when the lever 812 drives the ratchet 802 to rotate through the active pawl 813, the rotation direction and rotation amplitude are the same. A limiting mounting plate 822 is provided on the surface of partition 5, and an arc groove 823 is opened inside the limiting mounting plate 822. The inner end of the protrusion 821 is movably inserted into the arc groove 823. The setting of the limiting mounting plate 822 and the arc groove 823 limits the protrusion 821, so that the rotation path of the protrusion 821 will not deviate, thus improving the rotation effect of the active pawl 813.

[0045] Example 2:

[0046] Please see Figure 1 - Figure 3 As shown, the present invention also discloses a method for microbial co-culture, which specifically includes the following steps;

[0047] Step 1: Place the pre-cultured algae into the algae pond 2, and place various microbial culture carriers inside the carrier frame 7 to simulate the microbial survival and reproduction environment. Plant submerged plants at the bottom of the algae-eating insect culture pond 4 to provide dissolved oxygen for the water and a suitable growth environment for the algae-eating insects. Then place the frame 1 as a whole in the surface water environment or aquaculture pond without occupying land area.

[0048] Step 2: During the growth and reproduction of algae and the growth of submerged plants in algae pond 2, the water level in algae pond 2 and algae-eating insect cultivation pond 4 will drop. At this time, the water conveying device 11 will work to draw water from the outside from the bottom of the central cylinder 9 to replenish the algae pond 2. When replenishing water, the water level in the central cylinder 9 will rise and overflow the top of the central cylinder 9, so that the water will carry algae to the area between the partition 5 and the central cylinder 9. Then the water level here will rise, and the water flow carrying algae will flow from the bottom of the partition 5 into the microbial cultivation pond 3, replenishing the water and providing algae for the microbial cultivation pond 3. The algae can serve as food for organisms such as wasps and promote the reproduction of microorganisms.

[0049] Step 3: In the microbial culture tank 3, the water level drops due to environmental evaporation and microbial growth. Water is then replenished to raise the water level. Based on the buoyancy of the water, the transmission mechanism 8 is activated. As the water level in the microbial culture tank 3 drops, the float 803 descends accordingly. The float 803 then lowers one end of the lever 805 via the lifting rod 804, causing the other end of the lever 805 to rise. This, in turn, drives the toothed plate 810 upwards via the hinge shaft 807 and connecting plate 808. The upward movement of the toothed plate 810 drives the spur gear 811 and the actuating rod 812 to rotate. The lever 812 pushes the ratchet 802 to rotate slightly through the active pawl 813. The ratchet 802 then drives the carrier frame 7 to rotate slightly through the rotating shaft 20. When the water level rises to the initial state, the float 803 rises synchronously, driving the transmission mechanism 8 to work in reverse and reset. Due to the characteristics of the ratchet pawl 802, the carrier frame 7 will not rotate back in reverse. When the water level undergoes evaporation and water replenishment cycles, the transmission mechanism 8 will drive the carrier frame 7 to rotate periodically. Periodic exposure to air significantly increases the oxygen acquisition efficiency of aerobic microorganisms, while also helping to flush away the aging biofilm on the carrier surface and maintain high activity.

[0050] Step 4: The water in the microbial culture tank 3 can be discharged into the algae-eating insect culture tank 4 through the overflow port 13 to replenish the water in the algae-eating insect culture tank 4, so as to avoid the submerged plants from lacking water and affecting the submerged plants to provide dissolved oxygen to the water body, and to provide a suitable growth environment for the algae-eating insects.

[0051] Step 5: When microorganisms are needed for water purification in the external aquatic environment, the sealing plate 16 at the corresponding discharge outlet 15 can be opened, and the corresponding microorganisms and algae-eating insects can be selectively released into the environment. The cultivated algae, wasps, and algae-eating insects can also be used as feed for fish and shrimp, thus realizing ecological aquaculture.

[0052] The preferred embodiments of the present invention disclosed above are merely illustrative of the invention. These preferred embodiments do not exhaustively describe all details, nor do they limit the invention to any specific implementation. Clearly, many modifications and variations can be made based on the content of this specification. This specification selects and specifically describes these embodiments to better explain the principles and practical applications of the invention, thereby enabling those skilled in the art to better understand and utilize the invention. The invention is limited only by the claims and their full scope and equivalents.

Claims

1. A microbial co-culture device, comprising a frame (1), characterized in that, The frame (1) is arranged from the inside out as follows: an algae pond (2), a microbial culture pond (3), and an algae-eating insect culture pond (4). A central cylinder (9) is arranged in the middle of the algae pond (2). A partition (10) is arranged inside the lower end of the central cylinder (9). A water conveying device (11) is arranged in the middle of the partition (10). The water conveying device (11) has its pumping end located below and its draining end located inside the algae pond (2) above. A filtration mechanism is arranged inside the pumping end, with a filtration aperture of 0.01-200 mm. The algae pond (2) and the microbial culture pond (3) are separated by a partition (5). A sunroom (12) is arranged at the upper end of the partition (5). And the sunroom (12) is located above the algae pond (2). The microbial culture pond (3) and the algae-eating insect culture pond (4) are separated by partition two (6). The microbial culture pond (3) is equipped with a carrier frame (7). A transmission mechanism (8) is provided on one side of the carrier frame (7). The bottom of the frame (1) is equipped with filler (18). The lower end of the partition one (5) and the upper end of the filler (18) have a certain space, so that the algae pond (2) and the microbial culture pond (3) are connected through the partition one (5) and the filler (18). A submerged plant planting area (19) is provided above the filler (18) inside the algae-eating insect culture pond (4). The carrier frame (7) is rotatably installed inside the microbial culture tank (3) via a rotating shaft (20). The left and right ends of the rotating shaft (20) are rotatably connected to partition one (5) and partition two (6) respectively. The carrier frame (7) is provided with a wasp culture area (701), a microbial culture area (702) and a seaweed culture area (703) respectively. The wasp culture area (701), the microbial culture area (702) and the seaweed culture area (703) are all provided with carriers that match their culture environment. The transmission mechanism (8) includes a hollow mounting plate (801) and a ratchet (802). The ratchet (802) is disposed on the circumferential surface of the end of the rotating shaft (20). The hollow mounting plate (801) is disposed on the side of the partition (5) located in the microbial culture tank (3). A float (803) is disposed inside the upper end of the hollow mounting plate (801). A lifting rod (804) is disposed at the lower end of the float (803). A lever (805) is hinged to the lower end of the lifting rod (804). A connecting plate (808) is hinged to the other end of the lever (805) through a hinge pin (807). The upper end of the connecting plate (808) A toothed plate (810) is provided at the end. A spur gear (811) is meshed with the side of the upper end of the toothed plate (810). A toggle rod (812) is provided inside the spur gear (811). An active pawl (813) is fixedly connected to the end of the toggle rod (812) through a protrusion (821). The end of the active pawl (813) abuts against the teeth of the ratchet (802). A check pawl (814) abuts against the upper end of the ratchet (802). The check pawl (814) is rotatably mounted on the surface of the partition plate (5) through a rotating rod (815). A return spring (816) is provided on the circumferential surface of the rotating rod (815).

2. The microbial co-culture device according to claim 1, characterized in that, The inner sidewall of the hollow mounting plate (801) has a limiting groove (818), and a limiting block (817) is slidably installed inside the limiting groove (818). The limiting block (817) is fixedly connected to the lifting rod (804).

3. The microbial co-culture device according to claim 1, characterized in that, The lever (805) is rotatably mounted on the surface of the partition (5) via a fulcrum (806), and the fulcrum (806) is closer to the connection point between the lifting rod (804) and the lever (805). The connecting plate (808) has an movable groove (809) inside, and the end of the hinge (807) is inserted into the movable groove (809).

4. The microbial co-culture device according to claim 1, characterized in that, The surface of the partition (5) is provided with a positioning groove (501), and a positioning block (819) is slidably installed inside the positioning groove (501). The outer end of the positioning block (819) is fixedly connected to the toothed plate (810). The surface of the partition (5) is provided with an annular groove (502), and a positioning rod (820) is movably installed inside the annular groove (502). The outer end of the positioning rod (820) is fixedly connected to the surface of the actuating rod (812). The actuating rod (812) is sleeved on the surface of the rotating shaft (20). The center of the actuating rod (812) is the same as the center of the rotating shaft (20). The surface of the partition (5) is provided with a limiting mounting plate (822), and an arc groove (823) is provided inside the limiting mounting plate (822). The inner end of the protrusion (821) is movably inserted into the arc groove (823).

5. The microbial co-culture device according to claim 1, characterized in that, The partition 2 (6) is provided with an overflow port (13) inside, and the overflow port (13) is designed in a Z shape. The lower end of the overflow port (13) is the liquid inlet and is located inside the microbial culture tank (3). The upper end of the overflow port (13) is the liquid outlet and is located inside the algae-eating insect culture tank (4). A baffle (14) is provided next to the liquid inlet of the overflow port (13).

6. The microbial co-culture device according to claim 5, characterized in that, The rear side of the frame (1) is provided with a discharge port (15), and one is provided at each algae pond (2), microbial culture pond (3) and algae-eating insect culture pond (4). The outlet of the overflow port (13) is located above the discharge port (15). A sealing plate (16) is provided inside the discharge port (15). An electric telescopic rod (17) is provided at the upper end of the sealing plate (16), and the electric telescopic rod (17) is located inside the frame (1).

7. A method for microbial co-culture, applied to the microbial co-culture apparatus according to any one of claims 1-6, characterized in that, Specifically, the following steps are included: Step 1: Place the pre-cultured algae in the algae pond (2), place various microbial culture carriers inside the carrier frame (7), plant submerged plants at the bottom of the algae-eating insect culture pond (4), and then place the frame (1) as a whole in the surface water environment or aquaculture pond. Step 2: During the growth and reproduction of algae and the growth of submerged plants in the algae pond (2), the water level in the algae pond (2) and the algae-eating insect culture pond (4) will drop. At this time, the water conveying device (11) will draw water from the outside from the bottom of the central cylinder (9) to replenish the algae pond (2). When replenishing water, the water level in the central cylinder (9) will rise and overflow the top of the central cylinder (9), so that the water will carry algae to the area between the partition (5) and the central cylinder (9). Then the water level here will rise, and the water flow carrying algae will flow from the bottom of the partition (5) into the microbial culture pond (3). Step 3: The water level in the microbial culture tank (3) drops due to environmental evaporation and microbial growth. Then, the water level rises by replenishing water. Subsequently, the transmission mechanism (8) is driven to work based on the buoyancy of the water. The transmission mechanism (8) will drive the carrier frame (7) to rotate periodically. Step 4: Water in the microbial culture tank (3) can be discharged into the algae-eating insect culture tank (4) through the overflow port (13).

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