一种鉴定芒果热农1号和凯特杂种真实性的SNP分子标记及其应用
By designing an SNP molecular marker at locus 11253818 on mango chromosome 18 and applying specific primers, the difficulty of identifying the authenticity of hybrids in mango hybridization breeding was solved, enabling rapid and accurate hybrid identification and improving breeding efficiency.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- SOUTH SUBTROPICAL CROP RES INST CHINA ACAD OF TROPICAL AGRI SCI
- Filing Date
- 2025-10-15
- Publication Date
- 2026-07-17
AI Technical Summary
In mango hybridization breeding, it is difficult to identify the authenticity of the hybrids 'Renong No. 1' and 'Kate'. Existing methods are time-consuming, labor-intensive, and easily affected by environmental and human factors, making it difficult to accurately identify true and false hybrids.
SNP molecular markers were designed and applied. PCR reaction was performed using specific primers located at locus 11253818 on mango chromosome 18. The authenticity of the hybrid was determined by detecting the genotype. The primer sequences are shown in SEQ ID NO.1-3.
It enables rapid and accurate identification of the authenticity of mango hybrids 'Renong No. 1' and 'Kate', improving the efficiency and accuracy of hybrid identification and making it applicable to mango genetic breeding.
Smart Images
Figure CN121204281B_ABST