A composition for efficiently activating cellular energy and delaying photoaging, its preparation method and application.

Abstract

This invention discloses a composition for efficiently activating cellular energy and delaying photoaging, its preparation method, and its application, belonging to the field of cosmetic technology. The composition consists of recombinant collagen and anti-photoaging components, wherein the anti-photoaging components include resveratrol, kukui seed oil, *Imperata cylindrica* seed oil, and *Snow Algae* extract in a specific mass ratio. This composition can efficiently activate cellular energy and delay photoaging, effectively providing anti-aging, firming, wrinkle-reducing, antioxidant, soothing, repairing, and moisturizing effects. This invention enhances efficacy through synergistic effects of its components and has been tested to have high safety. It can be used to prepare various skincare products such as toners, lotions, and serums, providing a reliable technical solution for anti-photoaging skincare products.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, specifically relating to a composition for generating live cell energy and delaying photoaging, its preparation method, and its application. Background Technology

[0002] Photoaging (especially caused by UVA and UVB radiation) can lead to disordered energy metabolism in skin cells, loss of collagen, and breakage of elastic fibers, resulting in problems such as wrinkles, sagging, roughness, and damage to the skin barrier. It is also accompanied by oxidative damage caused by the accumulation of free radicals. Therefore, highly effective anti-photoaging products need to meet multiple needs such as "resisting light damage", "repairing the skin barrier", and "promoting collagen synthesis".

[0003] However, existing anti-photoaging skincare products have limited efficacy and poor synergy. Most products rely on a single active ingredient (such as adding only antioxidants or single plant extracts), and cannot simultaneously achieve the synergistic effects of resisting photodamage and repairing collagen. Summary of the Invention

[0004] To address the shortcomings of the existing technologies, this invention provides a composition that is comprehensive in efficacy, highly safe, can efficiently activate cellular energy, and delay photoaging by screening a synergistic formulation of recombinant collagen and anti-photoaging components and optimizing the preparation process of polar snow algae extract, thus filling the gap in the existing technologies.

[0005] To achieve the above objectives, the present invention discloses the following technical solutions: In a first aspect, the present invention provides a composition that efficiently activates cellular energy and delays photoaging, the composition containing recombinant collagen and anti-photoaging components; The anti-aging components consist of resveratrol, kukui seed oil, plantain leaf and blue thistle seed oil, and polar snow algae extract.

[0006] Preferably, the mass ratio of recombinant collagen to anti-photoaging component in the composition is (0.1-1):(0.1-3).

[0007] More preferably, the anti-photoaging component is composed of resveratrol, kukui seed oil, plantain leaf and blue thistle seed oil and polar snow algae extract in a mass ratio of (0.01-0.5):(1-5):(0.5-1):(0.2-5).

[0008] More preferably, the preparation method of the polar snow algae extract includes the following steps: Step 1: Add PBS buffer to polar snow algae and then perform cell disruption to obtain polar snow algae slurry. Filter the slurry to obtain a clear filtrate. Step 2: After centrifuging the filtrate, the exosome precipitate is obtained. The precipitate is resuspended with PBS buffer to obtain a suspension. The suspension is then concentrated by tangential flow filtration to obtain the exosome concentrate. Step 3: Dilute the exosome concentrate to a concentration of (1-10)×10⁻⁶. 9 The suspension was mixed with mannitol at a mass ratio of 100:(1-5) and the resulting mixture was freeze-dried to obtain the polar snow algae extract.

[0009] More preferably, in step 2, the centrifugation involves centrifuging the filtrate at 4000-6000×g for 25-35 min, then centrifuging at 15000-20000×g for 35-45 min, and finally ultracentrifuging at 100000-120000×g for 50-60 min to obtain exosome precipitate.

[0010] More preferably, the pore size of the filter for tangential flow filtration concentration is 30-400 nm.

[0011] Secondly, the present invention provides the application of the composition described in the first aspect in the preparation of skin care products with anti-aging, firming and anti-wrinkle, antioxidant, anti-photoaging, soothing and repairing, and moisturizing effects.

[0012] Thirdly, the present invention provides the application of the composition described in the first aspect in the preparation of lotions, emulsions, creams, masks, serums, sprays, lipsticks, lip essences, lip masks, lip creams, lip jelly, lip glosses, lip stains, and lipsticks with anti-aging, firming and anti-wrinkle, antioxidant, anti-photoaging, soothing and repairing, and moisturizing effects.

[0013] Fourthly, the present invention provides an essence that effectively activates cell energy and delays photoaging, wherein the essence contains the composition described in the first aspect; The composition is added to the serum in an amount of 0.2-4 wt%.

[0014] Preferably, the essence also contains thickeners, moisturizers, pH adjusters, emulsifiers, preservatives, fragrances, and solvents.

[0015] Fifthly, the present invention provides a method for preparing the serum described in the fourth aspect, comprising the following steps: The composition, thickener, moisturizer, pH adjuster, emulsifier, preservative, daily fragrance and solvent are stirred and mixed evenly to obtain the essence.

[0016] In this invention: Recombinant collagen, as a key structural protein in the dermis, can directly act on skin fibroblasts, activate cellular energy metabolism pathways, increase cellular ATP production, and provide an energy basis for collagen synthesis and cell repair.

[0017] Resveratrol, as a powerful free radical scavenger, can eliminate reactive oxygen species generated by UV radiation, inhibit lipid peroxidation, reduce free radical damage to cellular DNA and mitochondria, and block the initiation of photoaging from the source. At the same time, it can inhibit the release of inflammatory factors caused by photoaging, reduce skin redness, sensitivity and other photodamage reactions, and regulate the expression of anti-aging related genes to enhance cellular resilience.

[0018] Kukui seed oil is rich in unsaturated fatty acids and phytosterols, which can penetrate into the stratum corneum of the skin, replenish lipids, repair the skin barrier damaged by photoaging, reduce moisture loss, and improve skin tolerance. The oil matrix of kukui seed oil can serve as a carrier for dissolving and delivering resveratrol and polar snow algae extract, enhancing the skin permeability of fat-soluble active ingredients and preventing the inactivation of active ingredients.

[0019] Plantago asiatica seed oil contains a high proportion of linolenic acid, which can effectively relieve skin inflammation and discomfort caused by UV radiation. At the same time, it can promote the synthesis of ceramides in the stratum corneum, further strengthen the skin barrier, reduce the accumulation of photodamage, and work synergistically with resveratrol to enhance the efficiency of free radical scavenging and prolong the antioxidant effect.

[0020] Polar snow algae extract can activate the collagen synthesis pathway of skin cells, synergistically promote the synthesis of type I collagen by recombining collagen, and make up for the collagen loss caused by photoaging.

[0021] The beneficial effects of this invention are: 1. The anti-photoaging component provided by this invention can effectively promote the synthesis of type I collagen in fibroblasts, reverse the reduction in collagen synthesis in skin cells under light radiation, and upregulate the expression level of zebrafish elastin gene, thus having significant firming and anti-aging effects. 2. A 28-day efficacy test on human subjects showed that the serum containing the recombinant collagen and anti-photoaging components of this invention can effectively improve the skin elasticity R2 value, reduce the average volume and area of ​​wrinkles, and increase the water content in the stratum corneum. All indicators are far superior to those containing recombinant collagen alone, anti-photoaging components alone, or blank matrix, proving that the combination of recombinant collagen and anti-photoaging components achieves anti-aging, firming, and moisturizing effects. Attached Figure Description

[0022] To better illustrate the purpose, technical solution, and advantages of the present invention, the present invention will be further described below in conjunction with the accompanying drawings: Figure 1 Transmission electron microscopy image of exosomes from polar snow algae; Figure 2 This is a particle size distribution diagram of exosomes from polar snow algae. Detailed Implementation

[0023] To better illustrate the objectives, technical solutions, and advantages of this invention, the invention will be further described below with reference to specific embodiments. Those skilled in the art should understand that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

[0024] Unless otherwise specified, the test methods used in the examples and comparative examples are conventional methods; the materials and reagents used are commercially available unless otherwise specified; and the percentages mentioned in the examples and comparative examples are mass percentages unless otherwise specified.

[0025] In this invention: Recombinant collagen: Recombinant collagen types I, III, IV, VII, and XVII were compounded in a mass ratio of 1:1:1:1:1 and were all purchased from Jiangsu Chuangjian Medical Technology Co., Ltd. Resveratrol: Purchased from DSM Vitamins (Shanghai) Co., Ltd.; Kumquat seed oil: purchased from Xianting (Guangzhou) Trading Co., Ltd.; Plantago asiatica seed oil: purchased from Northstar Lipids (UK) Ltd; Polar snow algae: Chlamydomonas nivalis, purchased from Guangdong Zhuotai Pharmaceutical Co., Ltd.

[0026] Preparation of polar snow algae extract Step 1: Place the polar snow algae in a high-speed homogenizer, add PBS buffer at a material-to-liquid ratio of 1:1 g / mL, start the homogenizer to break down the tissue and obtain polar snow algae slurry. Pass the homogenate through a 200-mesh sieve for preliminary filtration to remove large tissue fragments and other impurities, and obtain a clear filtrate. Step 2: Centrifuge the filtrate at 5000×g for 30 min to remove larger cell fragments, then centrifuge at 17000×g for 40 min to further remove organelles and large vesicles. Finally, centrifuge at 100000×g for 60 min. After centrifugation, the exosomes from *Alternaria solani* will precipitate at the bottom of the tube. Discard the supernatant and resuspend the precipitate in pre-cooled PBS buffer to obtain a suspension. Concentrate the suspension by tangential flow filtration with a pore diameter of 30-700 nm to obtain the concentrated exosome solution (transmission electron microscopy image of *Alternaria solani* exosomes is shown below). Figure 1 As shown in the figure, the exosome concentrate was diluted a certain factor and then subjected to particle size distribution analysis. The results are as follows. Figure 2 As shown; Step 3: Dilute the exosome concentrate to a concentration of 1×10⁻⁶. 9 A suspension of 100 cells / mL was mixed with mannitol at a mass ratio of 100:4. The resulting mixture was then freeze-dried under vacuum to obtain the freeze-dried formulation, which is the polar snow algae extract.

[0027] Preparation of anti-photoaging components Weigh the raw materials precisely according to the mass ratio in Table 1, mix them evenly, and obtain the anti-photoaging component; Table 1 Resveratrol 0.01 0.1 0.5 / 0.1 0.1 0.1 Kumquat seed oil 1 3 5 3 / 3 3 Plantago asiatica seed oil 0.5 0.8 1 0.8 0.8 / 0.8 Polar snow algae extract 0.2 0.6 5 0.6 0.6 0.6 / Note: " / " in the table indicates no addition.

[0028] Performance testing 1. MTT testing (1) Cell seeding: at 2.2 × 10 4 Seed cells at a density of cells / well into 96-well plates and incubate overnight in an incubator (37°C, 5 v / v% CO2).

[0029] (2) Experimental grouping: The experiment set up a blank control group and an experimental group, and three cell-free wells were set up as zeroing wells. Five concentration gradients were set up for the samples in the experimental group, and three replicate wells were set up for each gradient concentration.

[0030] (3) Solution preparation: According to the experimental design, different concentrations of the test substance were prepared using basal culture medium.

[0031] Table 2 Test Concentration Setting Table

[0032] (4) Drug administration: 24 hours later, remove the 96-well plate, discard the old culture medium, add 100 μL of culture medium to each well of the blank control group, add 100 μL of culture medium containing the corresponding concentration of the sample to each well of the sample group; no cell seeding is done in the zeroing group, only 100 μL of cell culture medium is added. After drug administration, put the plate back into the incubator for continued culture.

[0033] (5) Detection: After culturing cells for 24 hours, discard the supernatant and add MTT working solution. Incubate at 37°C in the dark for 4 hours. After incubation, discard the supernatant, add 100 μL of DMSO to each well, and read the OD value at 490 nm.

[0034] (6) Calculation of relative cell viability: Calculated according to the formula,

[0035] (7) Cell viability test results Table 3 MTT test results

[0036] When the sample concentration is ≤1%, the cell viability is ≥90%, and further testing can be carried out within the concentration range of ≤1%.

[0037] 2. Effect test on type I collagen content (1) Inoculation: 2.2 × 10 4 Seed cells at a density of cells / well into 96-well plates and incubate overnight in an incubator (37°C, 5 v / v% CO2).

[0038] (2) UVA irradiation: After washing cells with PBS, according to the experimental groups, the groups exposed to UVA were subjected to 30 J / cm² irradiation. 2 UVA irradiation.

[0039] (3) Solution preparation: Based on the cell viability test results obtained above, select an appropriate sample concentration as the test concentration and prepare the test sample solution. Use TGF-β1 (100 ng / mL) as a positive control for the experiment. Add 100 μL of sample to each well, and set up 3 replicates for each group. After drug administration, place the 96-well plate in a 37℃ incubator and incubate in the dark for 24 h.

[0040] Table 4 Experimental Groups

[0041] (4) Collection of cell supernatant: 24 h after drug administration, remove the 96-well plate and collect the cell supernatant of each sample group into a centrifuge tube. Centrifuge at 1000 rpm for 10 min, collect the supernatant and place it in a 1.5 mL centrifuge tube. Store at -20℃ for later use.

[0042] (5) ELISA kit to determine the concentration of type I collagen in cell supernatant: Take out the kit and the sample to be tested 30 minutes before the test, place them at room temperature before use, and strictly follow the kit instructions for the test operation.

[0043] (6) Data statistics Statistical analysis was performed using SPSS 19.0 statistical software to conduct descriptive statistics on the measurements in the test area. Two-tailed tests were used for all statistical methods, with a significance level of α=0.05.

[0044] (7) Test results Table 5 Results of Type I Collagen Content Detection Model comparison 46.133±1.784 / Blank (not damaged by UVA) 63.125±4.115 36.83% Positive (TGFβ1 - 100 ng / mL) 61.379±3.884 33.05% Component 1 57.461±4.601 24.56% Component 2 60.845±4.615 31.89% Component 3 60.072±4.163 30.21% Component 4 50.214±7.205* 8.85% Component 5 52.801±5.447* 14.45% Component 6 51.658±6.084* 11.98% Component 7 49.276±2.956* 6.81% Note: Compared with component 2, "*" indicates a significant difference (P < 0.05).

[0045] (8) Results Analysis: According to the test results in Table 5, components 1-3 can effectively reverse the inhibition of collagen synthesis in fibroblasts by UVA. Among them, the effect of component 2 in promoting type I collagen synthesis is close to that of the potent inducer TGF-β1, which proves that it can repair collagen loss caused by photoaging at the cellular level. The collagen growth rate of components 4-7 decreased significantly after the absence of any of the components such as resveratrol and kukui seed oil, indicating that the four anti-photoaging components need to work synergistically to achieve the best collagen-promoting effect. Resveratrol scavenge free radicals and reduce collagen degradation, kukui seed oil / plantago asiatica seed oil repairs the barrier and protects cell function, and polar snow algae extract activates the collagen synthesis pathway. None of them can be missing.

[0046] 3. Zebrafish Firming Efficacy Test Embryos from wild-type AB zebrafish at 4 days post-fertilization were selected and randomly assigned to 6-well plates, 30 embryos per well. The plates were divided into a normal control group, a positive control group, and a test substance group. The test substance consisted of fractions 1-7 diluted to 0.1 wt% with embryo culture medium, with three replicates per group. The buffer water in the 6-well plates was removed without harming the larvae, and 3 mL of the corresponding solution was quickly added to each well. The plate was then covered and incubated in a biochemical incubator at 28.5°C in the dark for 24 hours.

[0047] in, Normal control group: containing zebrafish embryos and embryo culture medium; Positive control group: containing zebrafish embryos and a 20 mg / mL L-carnosine solution prepared with embryo culture medium; Test group: test substance and zebrafish embryos.

[0048] Total RNA was extracted from zebrafish in each group using a rapid RNA extraction kit, and cDNA was synthesized. Quantitative real-time PCR was used to detect the Ct values ​​of the internal reference gene, target genes eln1 and eln2 in each group, as detailed in "T / ZHCA 015-2022 Evaluation of the Firming Efficacy of Cosmetics: Relative Expression Level of Elastin Gene in Zebrafish Juveniles". The relative gene expression levels of each group of samples are calculated using the following formula.

[0049] A = C(t) - C(t) 0 B = A0 - A1 Relative gene expression level = 2 B ; In the formula: A—the difference between the number of cycles of the target gene and the internal reference; C(t) — the number of cycles required for the target gene fluorescence signal to reach the set threshold; C(t)0 — the number of cycles required for the internal reference fluorescence signal to reach the set threshold.

[0050] B—The difference between the average value of A in the blank control group and the value of A in the test substance; A0 – The mean difference between the target gene and the internal reference cycle number in the blank control group; A1 – The difference between the number of target genes and the number of internal reference cycles in the test group.

[0051] The results are shown in Table 6.

[0052] Table 6

[0053] Note: "#" indicates a significant difference compared to the blank control group, P < 0.05; "*" indicates a significant difference compared to component 2, P < 0.05.

[0054] Results analysis: Elastin (encoded by the eln1 and eln2 genes) is a key structural protein for skin firmness. Components 1-3 can significantly upregulate the expression of eln1 and eln2 genes in zebrafish juveniles, proving that the anti-photoaging components provided by this invention can promote elastin synthesis to achieve a firming effect. When any of the anti-photoaging components was missing from components 4-7, the expression level of elastin genes was significantly reduced, further demonstrating that the quaternary components of resveratrol, kukui seed oil, plantain leaf blue thistle seed oil, and polar snow algae extract need to work synergistically to activate the elastin synthesis pathway.

[0055] Preparation of application examples The preparation method for a serum that effectively activates cell energy and delays photoaging is as follows: Step 1: Accurately weigh the raw materials according to the raw material mass percentage in Table 7; Step 2: Add deionized water to a container, then add butanediol and glycerol in sequence. Stir at 300 rpm until completely dissolved. Add xanthan gum and ammonium acryloyl dimethyl taurate / VP copolymer, and heat to 75±2℃. Keep stirring at this temperature for 15 min to form a homogeneous and transparent solution, which is solution A. Step 3: Mix the anti-photoaging component 2 and PEG-20 methyl glucosesquistearate, heat to 65±2℃, stir until homogeneous, and form a transparent solution to obtain solution B; Step 4: Stabilize the temperature of solution A at 75℃, start high-speed homogenization, slowly add the preheated solution B to solution A, homogenize for 10 minutes to form a uniform milky white emulsion, then lower the temperature to 40℃, add recombinant collagen and 1,2-hexanediol, stir to disperse evenly, add fragrance and pH adjuster, adjust the pH to 6.5-7, filter and bottle to obtain the essence that effectively activates cell energy and delays photoaging.

[0056] Table 7. Application Examples: Raw Material Mass Percentage (Unit: %) Recombinant collagen 1 1 / / Anti-photoaging component 2 3 / 3 / PEG-20 Methyl Glucosamine Sesquistearate 0.7 0.7 0.7 0.7 Xanthan Gum 0.2 0.2 0.2 0.2 Acryloyldimethyltaurate ammonium / VP copolymer 0.1 0.1 0.1 0.1 Butylene glycol 4 4 4 4 glycerin 3 3 3 3 1,2-Hexanediol 0.1 0.1 0.1 0.1 pH adjuster Appropriate amount Appropriate amount Appropriate amount Appropriate amount Daily fragrance Appropriate amount Appropriate amount Appropriate amount Appropriate amount Deionized water Add to 100 Add to 100 Add to 100 Add to 100 Note: " / " in the table indicates no addition.

[0057] Human efficacy test (1) Test substance: the essence of the application example, comparative example 1, comparative example 2, and blank example. (2) Volunteer selection: The selection of subjects followed the medical and ethical standards for human testing. All subjects must participate in the test voluntarily and sign an informed consent form before the test. Forty healthy female subjects aged 18 to 40 years were recruited and randomly divided into four groups of 10 each.

[0058] (3) Method of using the experimental samples: After cleansing their face in the morning and evening, the subjects used the serums of the application example, comparative example 1, comparative example 2 and blank example respectively. Each time, 0.5g of serum was used and gently massaged until absorbed. Other anti-wrinkle skin care products were discontinued during the experiment.

[0059] (4) Before and 28 days after the use of the sample, the subjects’ facial images were collected using VISIA-CR, and the subjects’ facial skin roughness SEr and skin smoothness SEsm were collected using VC20 Plus. The moisture content of the stratum corneum of the cheeks was measured using the Corneometer CM 825 of CK Instruments in Germany. The changes in values ​​before and after the use of the sample were compared.

[0060] Before the test, the subjects washed their faces with the same facial cleanser and stabilized their skin in a constant temperature and humidity (temperature: 21 ℃ ± 1℃, humidity: 50% ± 10%) room for 20 minutes to maintain skin stability before the above test was performed.

[0061] (5) The results are shown in the table below: Change rate after product use = (Data after use - Data before use) / Data before use × 100%.

[0062] Table 8. Results of the rate of change of various skin parameters after 28 days. Application examples Increased by 8.45% Reduced by 12.63% Reduced by 15.51% Increased by 22.85% Comparative Example 1 Increased by 2.19% Reduced by 6.51% Reduced by 6.94% Increased by 10.29% Comparative Example 2 Increased by 3.27% Decrease of 4.28% Reduced by 5.34% Increased by 12.15% Blank example Increased by 0.22% Decrease by 0.16% Reduced by 0.74% Increased by 2.73% Results analysis: The experimental results show that the application example is significantly better than the comparative examples 1 and 2 and the blank example in all aspects, which proves that the recombinant collagen and the anti-photoaging components can achieve multi-dimensional and multi-pathway skin firming and anti-aging effects, and there is a certain synergistic effect. The recombinant collagen activates cell energy and promotes collagen synthesis, while the anti-photoaging components resist UV damage and repair the barrier, together achieving improved elasticity, reduced wrinkles and enhanced moisturization.

[0063] During the 28-day testing period, no adverse reactions such as redness, swelling, or stinging were observed in any of the subjects, and the efficacy indicators continued to improve, proving that it has both safety and long-lasting effects in actual skincare scenarios and can meet consumers' needs for combating photoaging and multi-effect repair.

[0064] Finally, it should be noted that the above descriptions are merely preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A composition for activating cellular energy and delaying photoaging, characterized in that, The composition comprises recombinant collagen and anti-photoaging components; The mass ratio of the recombinant collagen to the anti-photoaging component is (0.1-1):(0.1-3). The recombinant collagen is obtained by compounding recombinant collagen types I, III, IV, VII, and XVII in a mass ratio of 1:1:1:1:1; The anti-photoaging component is composed of resveratrol, kukui seed oil, plantain leaf and blue thistle seed oil and polar snow algae extract in a mass ratio of (0.01-0.5):(1-5):(0.5-1):(0.2-5); The preparation method of the polar snow algae extract includes the following steps: Step 1: Add PBS buffer to polar snow algae and then perform cell disruption to obtain polar snow algae slurry. Filter the slurry to obtain a clear filtrate. Step 2: Centrifuge the filtrate at 4000-6000×g for 25-35 min, then centrifuge at 15000-20000×g for 35-45 min, and finally ultracentrifuge at 100000-120000×g for 50-60 min to obtain exosome precipitate. Resuspend the precipitate in PBS buffer to obtain a suspension, and concentrate it by tangential flow filtration to obtain exosome concentrate. Step 3: Dilute the exosome concentrate to a concentration of (1-10)×10⁻⁶. 9 The suspension of 100 cells / mL was mixed with mannitol at a mass ratio of 100:(1-5), and the resulting mixture was freeze-dried to obtain the freeze-dried preparation, which is the polar snow algae extract.

2. The use of the composition of claim 1 in the preparation of skin care products with firming, anti-wrinkle, soothing, repairing, and moisturizing effects.

3. The use of the composition of claim 1 in the preparation of lotions, creams, masks and sprays having firming, anti-wrinkle, soothing and repairing, and moisturizing effects.

4. The use of the composition of claim 1 in the preparation of serums and lotions with firming, anti-wrinkle, soothing, repairing, and moisturizing effects.

5. The use of the composition of claim 1 in the preparation of lipsticks, lip serums, lip masks, lip creams, lip jelly, lip glosses and lip stains having firming, anti-wrinkle, soothing, repairing and moisturizing effects.

6. An essence that activates cellular energy and delays photoaging, characterized in that, The serum contains the composition according to claim 1; The composition is added to the serum in an amount of 0.2-4 wt%.

7. The essence according to claim 6, characterized in that, The essence also contains thickeners, moisturizers, pH adjusters, emulsifiers, preservatives, fragrances, and solvents.

8. The method for preparing the essence according to claim 7, characterized in that, Includes the following steps: The composition, thickener, moisturizer, pH adjuster, emulsifier, preservative, daily fragrance and solvent are stirred and mixed evenly to obtain the essence.

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