Plant endophytic branch fungus with drought resistance, growth promotion and insect resistance functions, microbial inoculant and application thereof

Abstract

The present application relates to the field of microorganisms, and particularly to a plant leaf endophytic cladosporium with drought resistance, growth promotion and insect resistance functions, a microbial inoculant and application thereof.The present application provides cladosporium endophyticum, and the preservation number is CGMCC No.42248.The present application aims at the industry pain point of fragmented functions of existing microbial preparations, and obtains a single strain with four functions of salt tolerance, drought resistance, growth promotion and insect killing through screening, thereby breaking through the limitations of the prior art, such as the salt tolerance and growth promotion function of the stress-resistant strain (Kushna bacteria HH-2), the drought resistance and growth promotion function of the growth-promoting strain (Bacillus cereus F06) and the functional fragmentation of the insecticidal strain, and solving the problems of high cost and poor compatibility of multiple preparations.In addition, the preservation is carried out at room temperature, the shelf life is long, and the preservation cost is low.

Description

Technical Field

[0001] This invention relates to the field of microorganisms, and more particularly to an endophytic cladoceran fungus on plant leaves with drought-resistant, growth-promoting and insect-resistant functions, a microbial agent and its application. Background Technology

[0002] Against the backdrop of global climate change and ecological degradation, agricultural production is facing three core challenges: soil salinization, drought stress, and pest and disease infestation. According to statistics from the Food and Agriculture Organization of the United Nations, the global area of ​​saline-alkali land has exceeded 1 billion hectares, with arid and semi-arid regions accounting for 41% of the total land area. Furthermore, crop yield losses caused by lepidopteran pests (such as corn borers and cotton bollworms) can reach 15% to 30%. In this context, environmentally friendly microbial technologies have become a key direction for promoting sustainable agricultural development. Currently, relevant known technologies mainly focus on the following three areas:

[0003] Firstly, there is the technology of stress-resistant microorganisms. Researchers have isolated various salt- and drought-tolerant strains. For example, Bacillus pumilus developed by Qingdao Weilan Biotechnology Group can promote crop growth and alleviate salt damage under high-salt conditions; Bacillus subtilis B21 significantly improves the survival rate and yield of cucumbers in saline-alkali land by regulating crop physiological metabolism. These strains mainly function by secreting osmotic regulators (such as proline) and improving the root microenvironment, but they usually do not have growth-promoting or insecticidal functions.

[0004] Second, there are plant growth-promoting microbial technologies. Growth-promoting strains enhance crop yields through mechanisms such as nitrogen fixation, phosphorus solubilization, and auxin secretion. For example, Bacillus (Adj 1) and Priestia aryabhattai (Adj 6) isolated from agave can secrete indoleacetic acid (up to 10.00 µg·mL⁻¹) and dissolve zinc, significantly increasing wheat yield under salt stress. The synergistic technology of Rhizobium and Erwinia developed by Gansu Agricultural University increased the fresh weight of alfalfa by 3.8 times through nitrogen fixation and extracellular polysaccharide secretion. However, the activity of these strains is easily affected under adverse conditions and they lack pest control capabilities.

[0005] Thirdly, there is microbial insecticide technology. Represented by Bacillus thuringiensis (Bt), its Cry toxin can specifically kill target pests, such as underground pests, showing good control effects. Some strains of Bacillus subtilis can also produce nematicides and insecticidal metabolites, used to control corn root borers. However, existing insecticidal strains mostly target single pest groups, and their insecticidal activity significantly decreases under stress environments such as salinity, alkalinity, and drought.

[0006] In summary, although the three types of microbial technologies mentioned above are relatively mature in their respective functional areas, they still have limitations such as single function and difficulty in synergy. In practical applications, using different formulations separately not only increases costs but may also affect efficacy due to antagonistic effects between strains, making it impossible to effectively address the complex environmental stresses commonly encountered in agricultural production. Summary of the Invention

[0007] In view of this, the present invention provides a plant leaf endophytic herbaceous fungus with drought-resistant, growth-promoting, and insect-resistant functions, a microbial inoculant, and its application. Addressing the industry pain point of fragmented functions in existing microbial preparations, this invention obtains a single strain that naturally integrates four functions: salt tolerance, drought resistance, growth promotion, and insecticidal properties. This overcomes the limitations of existing technologies where stress-resistant strains (Kushnerella HH-2 with salt tolerance and growth promotion function), growth-promoting strains (Bacillus cereus F06 with drought resistance and growth promotion function), and insecticidal strains have separate functions, solving the problems of high cost and poor compatibility when using multiple formulations in combination. Furthermore, it has a long shelf life and low storage cost when stored at room temperature.

[0008] To achieve the above-mentioned objectives, the present invention provides the following technical solution:

[0009] This invention provides Cladosporium endophyticum, with accession number CGMCCNo.42248.

[0010] The present invention also provides microbial inoculants, comprising any one of the following and acceptable adjuvants:

[0011] (a) the aforementioned Cladosporium endophyticum; and / or

[0012] (b) Inactivated Cladosporium endophyticum; and / or

[0013] (c) Metabolites, derivatives, fermentation broth, cultures, exosomes, lysates, or extracts of the aforementioned Cladosporium endophyticum.

[0014] The present invention also provides the application of the above-mentioned Cladosporium endophyticum and / or the above-mentioned microbial agents in improving plant drought resistance and / or alleviating plant drought stress.

[0015] The present invention also provides the application of the above-mentioned Cladosporium endophyticum and / or the above-mentioned microbial agents in the prevention and / or killing of lepidopteran pests.

[0016] In some embodiments of the present invention, the lepidopteran pests mentioned above include one or more of the following: diamondback moth, beet armyworm, cotton bollworm, and armyworm.

[0017] In some embodiments of the present invention, in the above applications, the application concentration of the Cladosporium endophyticum and / or the microbial agent is 10. 2 CFUs / L ~10 4 CFUs / L.

[0018] The present invention also provides the application of the above-mentioned Cladosporium endophyticum and / or the above-mentioned microbial agents in increasing yield and / or promoting plant growth and development.

[0019] The present invention also provides the application of the above-mentioned Cladosporium endophyticum and / or the above-mentioned microbial agents in improving the salt tolerance of plants and / or alleviating salt stress in plants.

[0020] The present invention also provides a method for preventing and / or killing lepidopteran pests by applying the above-mentioned Cladosporium endophyticum and / or the above-mentioned microbial agents to plants.

[0021] In some embodiments of the present invention, the concentration of the substance applied in the above method is 10. 2 CFUs / L ~10 4 CFUs / L.

[0022] The beneficial effects of this invention include:

[0023] (1) The strain provided by this invention can significantly enhance crop growth. After soil application treatment, the plant height increased by 23.07%, the plant fresh weight increased by 33%, the root development increased by 6.46%, and the root fresh weight increased by 44.16%. After continuous drought stress in the greenhouse, the survival rate of the treated group was significantly increased by about 10 times compared with the control group.

[0024] (2) It is effective against two types of lepidopteran larvae that pose a high risk to agriculture in my country. Among them, it shows high insecticidal activity against the young larvae of the beet armyworm (corrected mortality rate ≥81.67%) and the young larvae of the diamondback moth (corrected mortality rate ≥40.0%). The mortality rate against non-target natural enemies is <5%, and the coverage is suitable for planting multiple crops such as grains and vegetables, without the need to change the formulation multiple times.

[0025] (3) Through the dual action of “intestinal intake and intestinal wall penetration”, the corrected mortality rate of beet armyworm larvae is ≥81.67% and the mortality rate ≥50% within 48 hours after contact. A single application can control the pest population below the economic harm level, reducing the number of applications and labor costs.

[0026] (4) To address the "poor adaptability to extreme environments": to overcome the limitations of existing insecticidal strains in saline-alkali and arid environments that have "low survival rate and reduced insecticidal efficacy", and to provide a functional strain with excellent stress resistance. It can effectively survive on slopes with a salt content of 15% NaCl, and while effectively improving the survival rate of plants under drought stress, it can significantly promote the growth and development of the above-ground and underground parts of plants, and can be adapted to the agricultural production needs of special production areas such as saline-alkali land and arid areas.

[0027] (5) Strong ecological adaptability to plants. The core advantage of this invention lies in the endophytic origin of the selected cladoceran strain from plant leaves. This characteristic enables it to exhibit higher colonization competitiveness and ecological adaptability than soil-derived microorganisms in application, overcoming the common problem that the efficacy of traditional microbial agents is constrained by region and planting environment. Thanks to its endophytic affinity, this strain ensures the reliable expression of its multiple beneficial functions throughout the entire crop growth period. This characteristic makes it possible to develop highly efficient and stable standardized microbial pesticides and fertilizers that are unaffected by variations in the field environment, and has significant industrial application value.

[0028] (6) Long shelf life and low storage requirements. General live bacterial agents require low-temperature storage and have a short shelf life. A spore suspension stored at 4˚C in a 30% glycerol solution showed a spore germination rate of approximately 85.24% after two years. Spore powder stored at 4˚C showed a spore germination rate of approximately 82.17% after two years. Spore powder stored at room temperature for 8 months maintained a spore germination rate of approximately 83.8%. The requirements for long-term storage conditions and methods are extremely low.

[0029] Biological Preservation Instructions

[0030] Biological material: NP17; Classification name: Cladosporium endophyticum; Deposited on October 17, 2025 at the China General Microbiological Culture Collection Center (CGMCC); Address: Institute of Microbiology, Chinese Academy of Sciences, No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing; Accession number: CGMCC No. 42248. Attached Figure Description

[0031] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the accompanying drawings used in the description of the embodiments or the prior art will be briefly introduced below.

[0032] Figure 1The image shows the electrophoresis results of the PCR amplification products and the comparison results with the GenBank database. The top image shows the sample to be tested, which shows a single band with no obvious impurities. The band is clear and bright. From left to right in the image, the amplification results are: the sample 2000bp Marker, the sample to be tested in this invention, and the 2000bp Marker. The bottom image shows the BLAST comparison results of the search sequence in NCBI GenBank.

[0033] Figure 2 The effects of bacterial strain treatment on the growth and development of pea plants are shown in Figures A-D, where A represents plant height, B represents root length, C represents plant fresh weight, and D represents root fresh weight (the Y-axis represents the mean ± standard error). Seed inoculation with a concentration of 10... 5 CFUs / L spore suspension;

[0034] Figure 3 The effect of bacterial strain treatment on pea germination rate;

[0035] Figure 4 The effect of bacterial strain treatment on the survival rate of pea plants after drought stress was shown.

[0036] Figure 5 The effects of bacterial strains and bacterial concentrations on the mortality rate of lepidopteran larvae are shown in Figures A-D, where A represents the beet armyworm, B the cotton bollworm, C the diamondback moth, and D the average lethality rate of armyworm larvae (the Y-axis represents the mean larval mortality rate ± standard error). NP1 and NP10 represent individual feeds at a concentration of 10⁻⁶ ppm. 4 CFUs / L and 10 2 The bacterial suspension at CFUs / L served as a negative control, with no bacterial suspension added.

[0037] Figure 6 The effects of bacterial strains and bacterial concentrations on the survival (median survival time) of lepidopteran larvae are shown in Figures A-D, where: A represents the beet armyworm; B the cotton bollworm; C the diamondback moth; and D represents the average mortality rate of armyworm larvae (legend: mean larval mortality rate ± standard error). NP1 and NP10 represent individual feeds at a concentration of 10⁻⁶ ppm. 4 CFUs / L and 10 2 The bacterial suspension at CFUs / L served as a negative control, with no bacterial suspension added.

[0038] Figure 7 The image shows the pure culture of the Cladosporium strain in PDA solid medium; where: A shows the front of the colony; B shows the back of the colony. Detailed Implementation

[0039] This invention discloses an endophytic cladoceran fungus on the leaves of a plant with drought-resistant, growth-promoting, and insect-resistant functions, a microbial agent, and their applications.

[0040] It should be understood that the expression “one or more of…” individually includes each of the objects described after the expression, as well as various different combinations of two or more of the described objects, unless otherwise understood from the context and usage. The expression “and / or” combined with three or more described objects should be understood to have the same meaning, unless otherwise understood from the context.

[0041] The terms “including,” “having,” or “containing,” including the use of their grammatical synonyms, should generally be understood as open-ended and non-restrictive, for example, not excluding other unstated elements or steps, unless otherwise specifically stated or understood from the context.

[0042] It should be understood that the order of the steps or the order in which certain actions are performed is not important as long as the invention remains operational. Furthermore, two or more steps or actions can be performed simultaneously.

[0043] The use of any and all instances or exemplary language such as “e.g.” or “including” in this document is merely intended to better illustrate the invention and is not intended to limit the scope of the invention unless the claims are made. No language in this specification should be construed as indicating that any unclaimed element is essential to the practice of the invention.

[0044] Furthermore, the numerical ranges and parameters used to define the present invention are approximate values, and the relevant values ​​in the specific embodiments have been presented as precisely as possible. However, any value inevitably contains standard deviations due to individual test methods. Therefore, unless explicitly stated otherwise, it should be understood that all ranges, quantities, values, and percentages used in this disclosure are modified with the word "approximately". Here, "approximately" generally means that the actual value is within plus or minus 10%, 5%, 1%, or 0.5% of a specific value or range.

[0045] In Examples 1 to 5 of this invention, all raw materials and reagents used can be purchased from the market.

[0046] The present invention will be further illustrated below with reference to the embodiments:

[0047] Example 1: Methods and procedures for isolating and screening salt-tolerant strains

[0048] Healthy, undamaged plant leaf samples were collected from alfalfa fields in the northern irrigation area of ​​Yinchuan, placed in sterile bags, and brought back to the laboratory. After rinsing the leaf samples under running water to remove surface debris, they were then immersed in 70% ethanol for 2 minutes, followed by immersion in 1% sodium hypochlorite solution for 2 minutes. In a laminar flow hood, the samples were transferred to sterile water and rinsed 3-5 times. Under sterile conditions, the leaves were cut into 0.5cm × 0.5cm pieces and transferred to PDA solid culture medium. After incubation at a constant temperature for 3-5 days, typical single colonies of different morphologies were picked, purified, and stored on slant at 4℃.

[0049] Example 2: Methods and Results for Strain Identification

[0050] Pure cultures of the strain were collected, and fungal DNA was extracted using a fungal genomic DNA extraction kit. The ITS region was amplified by PCR using primers: ITS5: 5'-GGAAGTAAAAGTCGTAACAAGG-3' (as shown in SEQ ID NO:2) and ITS4: 5'-TCCTCCGCTTATTGATATGC-3' (as shown in SEQ ID NO:3). The 20 μL PCR reaction mixture consisted of 10 μL 2×TaqMaster Mix, 1 μL upstream primer, 1 μL downstream primer, 1 μL template DNA, and ddH2O added to a final volume of 20 μL. PCR conditions were as follows: 95℃ for 5 min; 95℃ for 30 s, 56℃ for 30 s, 72℃ for 1 min, 30 cycles; 72℃ for 5 min, then incubated at 4℃. The amplified products were subjected to agarose gel electrophoresis.

[0051] Sequence results:

[0052] Cladosporium endophyticum NP17: (as shown in SEQ ID NO:1)

[0053] Example 3: Strain Cultivation and Inoculum Preparation

[0054] Cultivation was performed in the laboratory using PDA plates, without special cultivation conditions. Mature spores were collected using Tween-20 aqueous solution to prepare a spore suspension. A 10... 5 The spore suspension (CFUs / mL) should be stored at 4°C for later use. Before inoculating plants in the laboratory, dilute proportionally as needed.

[0055] Example 4 Biological Function Determination

[0056] (1) Screening of salt-tolerant strains

[0057] The strains were screened using selective medium containing 15% NaCl (v:v) PDA, and they grew normally.

[0058] (2) Screening of salt-tolerant growth-promoting strains

[0059] Use 1 mL of 10 5 Pea seeds were inoculated with a spore suspension at a concentration of CFUs / L to complete a plant growth experiment. The control group served as a negative control and did not receive any inoculum. Plants were cultured in an artificial climate chamber (25±3℃, photoperiod 16 L:8 D), watered every two days. Plant growth data, including plant height (cm), root length (cm), plant fresh weight (g), and root fresh weight (g), were collected 14 days after germination (e.g., ...). Figure 2 (As shown).

[0060] (3) Effect of strain on emergence rate.

[0061] Use 1 mL of 10 5 Pea seeds were inoculated with a spore suspension at a concentration of CFUs / L. Each treatment group had 36 biological replicates. Seed germination rates were statistically analyzed. The control group served as a negative control, without inoculation (e.g., [missing information]). Figure 3 (As shown).

[0062] (4) Effects of strains on drought stress in plants.

[0063] Use 1 mL of 10 5 Pea seeds were inoculated with a spore suspension at a concentration of CFUs / L. Each treatment group had 36 biological replicates. Drought stress was applied 14 days after emergence, followed by a 7-day watering halt, and then re-watering. Plant survival rates were recorded. The control group served as a negative control, without the addition of any inoculum (e.g., CFUs / L). Figure 4 (As shown).

[0064] Table 1. Statistical analysis results of plant growth and development in different treatment groups.

[0065]

[0066] Table 1 shows that after 10 5 After inoculating seeds with a spore suspension at a concentration of CFUs / L, plant growth information was collected 14 days after germination, including plant height (cm), root length (cm), plant fresh weight (g), root fresh weight (g), and germination rate (%). The data were recorded and statistically analyzed (P<0.05). Following the same seed treatment, drought stress was applied 14 days after germination, followed by a 7-day watering halt, and then re-watering. Plant survival rate (%) was then recorded.

[0067] Figure 2 Indicates 10 5 After inoculating seeds with a spore suspension at a concentration of CFUs / L, plant growth information was collected 14 days after germination, including statistical analysis results of plant height (A), root length (B), plant fresh weight (C), and root fresh weight (D). *This indicates a significant difference between the treatment group and the control group (P<0.05).

[0068] Figure 3 Indicates 10 5 After inoculating seeds with a spore suspension at a concentration of CFUs / L, the germination rate was calculated 14 days after germination. The control group used a spore-free solution. The germination rate of the control group was 72.22%, while that of the treatment group was 97.22%.

[0069] Figure 4 Indicates 10 5 Seeds were inoculated with a spore suspension at a concentration of CFUs / L. Drought stress was applied 14 days after emergence, followed by a 7-day watering halt, followed by rewatering. Plant survival rates (%) were then recorded. The control group received a spore-free solution. The survival rate of the control group was 8.33%, while the survival rate of the treatment group was 91.67%.

[0070] (5) Screening out some functional strains with dual resistance to salt and insects.

[0071] Two bacterial suspension concentration gradients were set up to screen the insecticidal resistance of bacterial strains against seven lepidopteran insects, including the diamondback moth (Plutella xylostella), beet armyworm (Spodoptera exigua), cotton bollworm (Helicoverpa armigera), armyworm (Mythimna separata), fall armyworm (Spodoptera frugiperda), corn borer (Ostrinia furnacalis), and beet armyworm (Spodoptera litura). Among these, the insecticidal resistance of the strains against the three major lepidopteran agricultural pests—fall armyworm, corn borer, and beet armyworm—was not ideal.

[0072] In experiments on the lethality of different bacterial strains against the larvae of four lepidopteran pests (diamond moth, beet armyworm, cotton bollworm, and armyworm) under different concentrations of bacterial agents, the insecticidal effects showed significant differences in lethality efficiency under different concentration gradient treatments (e.g., Figure 5 (As shown). Furthermore, survival curves of young lepidopteran larvae from four species (diamond moth, beet armyworm, cotton bollworm, and armyworm) were plotted under different feeding dosages to help evaluate the effects of different feeding dosages on the adaptability of lepidopteran larvae (e.g., ...). Figure 6 (As shown).

[0073] like Figure 5As shown in Table 2, the mortality rate (%) of the bacterial strains against the larvae of four lepidopteran pests—beet armyworm (A), cotton bollworm (B), diamondback moth (C), and armyworm (D)—under different bacterial agent concentrations was statistically analyzed. NP1 represents high concentration, and NP10 represents low concentration. Table 2 presents the statistical analysis results of larval mortality rates in different treatment groups.

[0074] like Figure 6 The survival curves and individual risk tables for four species of lepidopteran larvae—beet armyworm (A), cotton bollworm (B), diamondback moth (C), and armyworm (D)—under different feeding dosage treatments are shown. Figure 6 Figure A shows the survival curves of the beet armyworm, displaying three survival curves. The survival rate of group NP1 decreased rapidly over time, with a high mortality rate. Group NP10 was second only to group NP1, but significantly faster than the control group. The survival rate of the control group remained close to 100%. The differences in survival rates between groups were statistically significant (P < 0.0001). In the risk table, the number of remaining samples in each group decreased over time; specifically, the time points from 60 to 0 individuals in groups NP1 and NP10 were 2→4→6→8→10, while the control group remained at 60 individuals. Mortality was concentrated in the early stage (0-5 days), with group NP1 having the most deaths. The other three figures are similar.

[0075] Figure 6 Figure B shows the survival curves of the cotton bollworm. The survival rate of group NP1 decreased the fastest, falling below 50% around day 5. The survival rate of group NP10 initially decreased slowly, then accelerated, ultimately falling between that of group NP1 and the control group. The differences in the survival curves among the three groups were significant (P < 0.0001). The table of at-risk individuals shows that the sample size in group NP1 decreased sharply over time, leaving 0 individuals at time point 8. The sample size in group NP10 decreased more slowly, still having 44 individuals at time point 9. The control group had 60 individuals with no deaths throughout the process, indicating no sample loss. Deaths in group NP1 were concentrated between time points 5.0 and 7.5, indicating this period was a high-risk period. Deaths in group NP10 were more dispersed, while no deaths occurred in the control group.

[0076] Figure 6 C represents the survival curve of the diamondback moth. The survival rate of group NP1 decreased the fastest, while group NP10 had a moderate survival rate with a slower decline than NP1. The differences in the survival curves among the three groups were significant (P<0.0001). Group NP1 had the highest risk, with a rapid decline in survival rate, reaching only 32 cases (46.7% survival) at time point 4. Group NP10 had a moderate survival rate, between the control group and group NP1, but significantly better than group NP1 (60% survival rate at time point 4). The survival curves, consistent with the table of the number of at-risk individuals, indicate that group NP1 had the highest incidence of mortality.

[0077] Figure 6Figure D shows the survival curves of armyworms, displaying three survival curves. The survival rate of the NP1 group decreased rapidly over time (by day 5), with a high incidence of death. The NP10 group showed a slower decline, while the control group maintained a survival rate close to 100%. By the end of the observation period (approximately day 15), the NP1 group had the lowest survival rate (approximately 0.82), followed by the NP10 group (approximately 0.97), while the CK group maintained a 100% survival rate. The differences in survival rates between groups were significant (P < 0.0001). The survival curves, combined with the risk individual count, indicate that events in the NP1 group mostly occurred in the middle of the observation period (e.g., days 5-10).

[0078] Table 2. Statistical analysis results of larval mortality rate (%) in different treatment groups.

[0079]

[0080] Example 5: Spore activity assay

[0081] (1) Preservation and determination of glycerol solution

[0082] Take 1 mL of 10 10 A spore suspension of CFU / mL was thoroughly mixed with 19 mL of 30% glycerol solution in a sterile centrifuge tube. The samples were sealed and stored at 4°C. Samples were collected and analyzed after 0 months (initial), 6 months, 12 months, and 24 months of storage. For each sample collection, 0.1 mL of the storage solution was serially diluted with 10% Tween 20 aqueous solution to the appropriate concentration. 0.1 mL of the diluted solution was then spread onto plate counting agar and incubated at 37°C for 24 hours. The spore germination rate was then calculated. The formula for calculating the spore germination rate is:

[0083]

[0084] (2) Determination of spore powder preservation

[0085] Transfer the dried spore powder into sterile centrifuge tubes, seal them, and store them in a refrigerator at 4°C. Samples were taken at 0 months (initial), 6 months, 12 months, and 24 months after storage, with each sample being approximately 0.01 g. The samples were serially diluted with 10% Tween 20 aqueous solution to an appropriate concentration. 0.1 mL of the diluted solution was spread onto plate counting agar medium and incubated at 37°C for 24 h. The spore germination rate was then counted.

[0086] (3) Preservation test of spore powder at room temperature

[0087] Transfer 0.5g of dried spore powder into a sterile centrifuge tube, seal it, and store it in a dark environment at room temperature (20~25℃). Take samples after 8 months of storage, and use the same sampling and germination rate detection methods as above.

[0088] Table 3. Statistical analysis results of spore germination rate (%) under 4℃ storage environment.

[0089]

[0090] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. Cladosporium ( Cladosporium endophyticum ), characterized in that, Its accession number is: CGMCCNo.42248.

2. A microbial inoculant, characterized in that, include: The mycosporium as described in claim 1 ( Cladosporium endophyticum ).

3. The mycospora as described in claim 1 ( Cladosporium endophyticum The application of the microbial agents as described in claim 2 in improving the drought resistance of peas and / or alleviating drought stress in peas.

4. The mycospora as described in claim 1 ( Cladosporium endophyticum The application of the microbial agents as described in claim 2 in the control of lepidopteran pests; The lepidopteran pests mentioned are one or more of the following: diamondback moth, beet armyworm, cotton bollworm, and armyworm.

5. The application as described in claim 4, characterized in that, The mycosporum ( Cladosporium endophyticum The application concentration of the microbial agent is 10. 2 CFUs / L ~10 4 CFUs / L.

6. The mycospora as described in claim 1 ( Cladosporium endophyticum The application of the microbial agent as described in claim 2 in promoting the growth and development of peas.

7. A method for controlling lepidopteran pests, characterized in that, The mycosporum as described in claim 1 ( Cladosporium endophyticum ) and / or the microbial agent as described in claim 2, applied to pests; The lepidopteran pests mentioned are one or more of the following: diamondback moth, beet armyworm, cotton bollworm, and armyworm.

8. The method as described in claim 7, characterized in that, The applied concentration is 10. 2 CFUs / L ~10 4 CFUs / L.

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