一种快速可视化检测海鳗成分的引物组、试剂盒及方法
By designing a LAMP primer set for moray eels and a closed-tube detection method, the problems of rapid, economical, and specific identification of moray eel meat components were solved, enabling efficient moray eel detection in grassroots units and on-site, meeting the requirements of sensitivity and cost.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- 临沂市检验检测中心
- Filing Date
- 2025-12-01
- Publication Date
- 2026-07-17
AI Technical Summary
Existing technologies are insufficient for efficiently and economically identifying the components of eel meat in grassroots testing institutions and on-site rapid screening scenarios. Furthermore, existing methods are heavily reliant on sophisticated equipment and high-cost reagents, failing to meet the demand for rapid and convenient testing.
A set of LAMP primers based on the molecular genetic characteristics of moray eels was designed. Combined with loop-mediated isothermal amplification technology, a rapid and visual detection method was established. The moray eel components were identified by specific primers, and the results were determined by directly observing color changes using a closed-tube detection method.
It achieves highly specific and low-cost detection of eel components within 60 minutes, with a sensitivity of 1 ng, making it suitable for rapid on-site screening in grassroots units, avoiding aerosol contamination, and reducing equipment and reagent costs.
Smart Images
Figure FT_1 
Figure FT_2 
Figure FT_3