辣椒多分枝基因CaBr1连锁的KASP分子标记及其引物、试剂盒和应用

By developing a KASP molecular marker linked to the CaBr1 branching gene in chili peppers, and using specific primers and fluorescence signals for detection, the problem of low efficiency in chili pepper branching breeding was solved, enabling rapid and accurate branching identification and breeding, and promoting the breeding process for chili peppers with good plant type.

CN121249954BActive Publication Date: 2026-07-17HUNAN AGRI UNIV

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
HUNAN AGRI UNIV
Filing Date
2025-11-24
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

In existing technologies, the regulatory mechanisms of chili branching-related genes are unclear, resulting in low efficiency in chili branching breeding, long conventional breeding cycles, and susceptibility to environmental influences.

Method used

To develop a KASP molecular marker linked to the branching gene CaBr1 in chili peppers, PCR amplification using specific primers and KASP reagent detection will be performed. Fluorescence signals will be used to distinguish the branching morphology of chili peppers, providing a rapid identification and breeding-assisted method for chili pepper branches.

Benefits of technology

This method enables efficient identification and early screening of chili pepper branching morphology, shortens the breeding cycle, improves breeding efficiency and accuracy, and lays the foundation for the selection of good chili pepper plant types.

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Abstract

本发明属于辣椒种植技术领域,公开了辣椒多分枝基因CaBr1连锁的KASP分子标记及其引物、试剂盒和应用,该KASP分子标记是以辣椒S8(zhangshugang)为参考基因组,8号染色体上第103175415个碱基处的单核苷酸多态性,此处发生了碱基G到A的替换。利用BSA群体定位的方法获得与辣椒多分枝紧密连锁的染色体区域,并在候选区间内开发分子标记确定候选基因CaBr1,根据候选基因的碱基突变筛选到一个KASP分子标记,利用该标记对F2群体90个随机抽样的单株进行基因型鉴定,符合率达到100%。该结果不仅有助于辣椒分枝表型鉴别及辅助育种,且为分枝基因的图位克隆及解析辣椒株型调控的分子机理奠定了基础。
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