一种用于抑制流式细胞术串联染料降解的组合物及其应用
By using a combination of reactive oxygen species scavengers and NADPH oxidase inhibitors, the degradation of tandem dyes in flow cytometry was inhibited, solving the problem of easy degradation of tandem dyes, maintaining cell viability, and improving the quality of experimental data.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- BEIJING MAOYANG BIOTECHNOLOGY CO LTD
- Filing Date
- 2025-11-11
- Publication Date
- 2026-07-17
AI Technical Summary
Tandem dyes are easily degraded in flow cytometry due to light and fixation conditions, leading to weakened fluorescence signals and interference between adjacent channels, which affects the accuracy and reproducibility of the experiment. Current technologies for cell fixation either result in cell death or the high toxicity of sodium azide, which cannot meet the requirements of live cell experiments.
The study employs reactive oxygen species (ROS) scavengers such as dihydrolipoic acid (DHLA) and NADPH oxidase inhibitors such as VAS 2870, combined with superoxide dismutase mimics such as EUK-134. This approach aims to eliminate existing ROS and inhibit the generation of new ROS, thereby suppressing tandem dye degradation. Additionally, auxiliary components such as energy sources, protein stabilizers, and stress protectants are added to maintain cell viability.
It significantly reduces tandem dye degradation, maintains cell viability, enhances the stability of target channel signals, and ensures the accuracy and reproducibility of experimental results. It is suitable for a variety of cell samples and dyes and is applicable to multicolor flow cytometry.
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