Rubber undergrowth hill turtle stage-by-stage fertilization method

Abstract

The present application relates to rubber forest mountain turtle stage-by-stage fertilization method, belong to agricultural planting technical field, the present application provides a kind of rubber forest mountain turtle stage-by-stage fertilization method, including the following steps: selecting suitable rubber forest land fertilization land preparation, planting mountain turtle seedling, applying seedling fertilizer, compound fertilizer 1 is applied during the growth period of this year;Compound fertilizer 2 is applied during the growth period of second year, vine is topped, and humic acid aqueous solution is sprayed on leaf surface;Compound fertilizer 3 is applied during the growth period of third year, vine is pruned, and dihydrogen potassium phosphate aqueous solution is sprayed on leaf surface, and mountain turtle tuber is harvested during dormancy period.According to the method of the present application, mountain turtle is fertilized, the nutrient requirement of different growth stages of mountain turtle can be met, the combination of underground application of fertilizer, aboveground pruning and leaf spraying solution realizes the rapid growth of mountain turtle tuber and the accumulation of nutrients, and the yield and quality of mountain turtle are improved.

Description

Technical Field

[0001] This invention relates to the field of agricultural planting technology, and in particular to a method for staged fertilization of turtles in rubber forests. Background Technology

[0002] Mountain tortoise, a traditional Chinese medicinal herb, has tubers with medicinal value including clearing heat and detoxifying, relieving pain, and resolving phlegm and nodules. The tubers also contain ingredients for producing the non-narcotic analgesic, craniolone, which, compared to existing narcotic analgesics, is not addictive. Rubber forests, an important economic forest species in tropical and subtropical regions, have unused understory space. Intercropping rubber trees with mountain tortoises can improve land utilization and farmers' economic benefits, achieving a win-win ecological and economic model of "using short-term gains to support long-term development."

[0003] Currently, there are techniques for intercropping *Triplophysa grahami* in rubber plantations. However, *Triplophysa grahami* typically takes 3-5 years from sowing to harvest. Its growth cycle generally consists of a growing season and a dormant season, and the nutritional requirements vary depending on the age of the plant. Currently, most growers use a "one-size-fits-all" fertilization method without considering the differentiated nutritional needs of *Triplophysa grahami*, resulting in low quality and delayed harvesting. There is an urgent need for a fertilization method tailored to different stages of *Triplophysa grahami* growth to improve its quality and shorten the harvest time.

[0004] Based on this, the present invention is proposed. Summary of the Invention

[0005] The purpose of this invention is to provide a method for fertilizing wild tortoises in rubber forests in stages, so as to solve the problem that there is no method for fertilizing wild tortoises at different stages in the prior art.

[0006] To achieve the above-mentioned objectives, the present invention provides the following technical solution: This invention provides a method for staged fertilization of tortoises in rubber plantations, comprising the following steps: (1) Select rubber forest land with a canopy density of 0.4~0.6, apply base fertilizer and plow the forest land to obtain plowed forest land; (2) In spring, the seedlings of the mountain tortoise are planted in the tilled forest land at a density of 900-1100 seedlings / mu, with the distance between the seedlings and the roots of the rubber trees being 0.5-1.5m. (3) Apply seedling fertilizer every 15 to 25 days after planting, 1 to 3 times, with an application rate of 25 to 30 kg / mu each time; (4) Apply compound fertilizer every 25-35 days during the growing season, 3-5 times; apply 500-700 kg / mu of well-rotted sheep manure once during the dormant period; (5) Apply compound fertilizer 2 every 25-35 days during the second year’s growing season, 4-6 times; top the vines of the mountain tortoise every 15-25 days; spray the leaves with humic acid solution every 20-30 days, 3-5 times; apply 400-600 kg / mu of decomposed sheep manure once during the dormant period. (6) Apply compound fertilizer 3 every 25-35 days during the third year of growth, apply 3-5 times, prune the vines of the mountain tortoise every 15-25 days, spray the leaves with potassium dihydrogen phosphate solution every 20-30 days, and harvest the tubers of the mountain tortoise during the dormant period.

[0007] Preferably, the base fertilizer in step (1) is well-rotted animal manure; The amount of base fertilizer used is 1800~2600 kg / mu.

[0008] Preferably, the seedling fertilizer in step (3) comprises the following components in parts by weight: 3-5 parts superphosphate, 0.5-1.5 parts zinc sulfate, 9-11 parts humic acid, and 11-13 parts well-rotted cow manure.

[0009] Preferably, the compound fertilizer 1 in step (4) includes nitrogen, phosphorus and potassium elements, and the mass ratio of nitrogen, phosphorus and potassium elements is 1~2:1~2:1~2; the application amount of the compound fertilizer 1 each time is 80~90 kg / mu.

[0010] Preferably, the compound fertilizer 2 in step (5) includes nitrogen, phosphorus, potassium and silicon elements, and the mass ratio of nitrogen, phosphorus, potassium and silicon elements is 4~6:6~8:2~4:0.4~0.6; the application amount of the compound fertilizer 2 each time is 90~100kg / mu.

[0011] Preferably, the concentration of the humic acid aqueous solution in step (5) is 0.4~0.6wt%, and the spraying amount for each application is 45~55L / mu.

[0012] Preferably, the compound fertilizer 3 in step (6) includes nitrogen and phosphorus elements, and the mass ratio of nitrogen to phosphorus elements is 2~4:1~3; the application amount of the compound fertilizer 3 each time is 60~80kg / mu.

[0013] Preferably, the concentration of the potassium dihydrogen phosphate aqueous solution in step (6) is 0.1~0.3wt%, and the spraying amount for each application is 30~40L / mu.

[0014] Preferably, the pruning method in step (6) is to prune the vines of the mountain tortoise to 0.5~1.5 meters / s.

[0015] This invention provides the application of the method in breeding high-quality mountain turtles.

[0016] The present invention has the following technical effects and advantages: This invention provides a phased fertilization method for the three-year growth of *Trionyx sinensis*. In the first year, fertilization during the seedling stage ensures seedling growth. During the growing season, compound fertilizer is applied to guarantee the nutrients needed for the growth of *Trionyx sinensis*, promoting balanced development of its tubers and vines. In the second year, the compound fertilizer applied is supplemented with silicon to promote root growth. The element ratios and dosages of the compound fertilizer are also modified. Combined with pruning of the above-ground vines and foliar spraying, photosynthesis is enhanced, further accelerating the accumulation of nutrients in the roots. In the third year, above-ground pruning and foliar spraying promote the rapid transfer of nutrients from the above-ground parts to the underground. This three-year phased fertilization method achieves rapid tuber growth and nutrient accumulation in *Trionyx sinensis*, improving its yield and quality. Detailed Implementation

[0017] This invention provides a method for staged fertilization of tortoises in rubber plantations, comprising the following steps: (1) Select rubber forest land with a canopy density of 0.4~0.6, apply base fertilizer and plow the forest land to obtain plowed forest land; The canopy closure of the rubber plantation is preferably 0.5; (2) In spring, the seedlings of the mountain tortoise are planted in the tilled forest land at a density of 900-1100 seedlings / mu, with the distance between the seedlings and the roots of the rubber trees being 0.5-1.5m. The preferred planting density is 1000 plants per mu; the preferred distance between the mountain tortoise seedlings and the rubber tree roots is 1m; (3) Apply seedling fertilizer every 15 to 25 days after planting, 1 to 3 times, with an application rate of 25 to 30 kg / mu each time, preferably 28 kg / mu; The preferred time to apply seedling fertilizer to seedlings is every 20 days; the preferred number of applications is twice. (4) Apply compound fertilizer every 25-35 days during the growing season, 3-5 times; apply 500-700 kg / mu of well-rotted sheep manure once during the dormant period, preferably 600 kg / mu. The preferred application time for compound fertilizer 1 is every 30 days; the preferred number of applications is 4. (5) Apply compound fertilizer 2 every 25-35 days during the second year’s growing season, 4-6 times. Pinch the top of the vines every 15-25 days. Spray the leaves with humic acid solution every 20-30 days, 3-5 times. Apply 400-600 kg / mu of well-rotted sheep manure once during the dormant period, preferably 500 kg / mu. The preferred application time for compound fertilizer 2 is every 30 days; the preferred number of applications is 5 times; the preferred time for topping the tortoise vine is every 20 days; the preferred time for foliar spraying of humic acid aqueous solution is every 25 days; the preferred number of sprays is 4 times. (6) Apply compound fertilizer 3 every 25-35 days during the third year of growth, apply 3-5 times, prune the vines of the mountain tortoise every 15-25 days, spray the leaves with potassium dihydrogen phosphate solution every 20-30 days, and harvest the tubers of the mountain tortoise during the dormant period. The preferred time for applying compound fertilizer 3 is every 30 days; the preferred number of applications is 4 times; the preferred time for pruning the tortoise vine is every 20 days; and the preferred time for foliar spraying with potassium dihydrogen phosphate solution is every 25 days.

[0018] In this invention, the base fertilizer mentioned in step (1) is well-rotted animal manure, and the animal manure is one or more of cattle, sheep and chicken manure; The amount of base fertilizer used is 1800~2600 kg / mu, preferably 2200 kg / mu.

[0019] In this invention, the seedling fertilizer in step (3) comprises the following components in parts by mass: 3-5 parts superphosphate, 0.5-1.5 parts zinc sulfate, 9-11 parts humic acid, and 11-13 parts well-rotted cow manure; The preferred mass fraction of the superphosphate is 4 parts; the preferred mass fraction of the zinc sulfate is 1 part; the preferred mass fraction of the humic acid is 10 parts; and the preferred mass fraction of the decomposed cow manure is 12 parts.

[0020] In this invention, the compound fertilizer 1 in step (4) includes nitrogen, phosphorus and potassium elements, and the mass ratio of nitrogen, phosphorus and potassium elements is 1~2:1~2:1~2, preferably 1:1:1; the application amount of the compound fertilizer 1 each time is 80~90kg / mu, preferably 85kg / mu.

[0021] In this invention, the compound fertilizer 2 in step (5) includes nitrogen, phosphorus, potassium and silicon elements, and the mass ratio of nitrogen, phosphorus, potassium and silicon elements is 4~6:6~8:2~4:0.4~0.6, preferably 5:7:3:0.5; the application amount of the compound fertilizer 2 each time is 90~100kg / mu, preferably 95kg / mu.

[0022] In this invention, the concentration of the humic acid aqueous solution in step (5) is 0.4~0.6wt%, and the spraying amount for each application is 45~55L / mu, preferably 50L / mu; The concentration of the humic acid aqueous solution is preferably 0.5 wt%.

[0023] In this invention, the compound fertilizer 3 in step (6) includes nitrogen and phosphorus elements, and the mass ratio of nitrogen to phosphorus elements is 2~4:1~3, preferably 3:2; the application amount of the compound fertilizer 3 each time is 60~80kg / mu, preferably 70kg / mu.

[0024] In this invention, the concentration of the potassium dihydrogen phosphate aqueous solution in step (6) is 0.1~0.3wt%, and the spraying amount for each application is 30~40L / mu, preferably 35L / mu; The concentration of the potassium dihydrogen phosphate aqueous solution is preferably 0.2 wt%.

[0025] In this invention, the pruning method in step (6) is to prune the vines of the mountain tortoise to 0.5~1.5 meters / s, preferably 1 meter / s.

[0026] This invention provides the application of the method in breeding high-quality mountain turtles.

[0027] The technical solutions provided by the present invention will be described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0028] Example 1

[0029] Preparation of seedling fertilizer: Mix 20 kg of superphosphate, 5 kg of zinc sulfate, 50 kg of humic acid and 60 kg of well-rotted cow manure evenly, crush them, and granulate them to obtain seedling fertilizer for later use.

[0030] Compound Fertilizer 1: Mix ammonium chloride, superphosphate and potassium chloride evenly in a mass ratio of nitrogen, phosphorus and potassium of 1:1:1 to obtain Compound Fertilizer 1, and set aside.

[0031] Compound Fertilizer 2: Mix ammonium chloride, superphosphate, potassium chloride and sodium silicate evenly in a mass ratio of nitrogen, phosphorus, potassium and silicon of 5:7:3:0.5 to obtain Compound Fertilizer 2, and set aside.

[0032] Compound fertilizer 3: Mix ammonium chloride and superphosphate evenly in a nitrogen-to-phosphorus ratio of 3:2 to obtain compound fertilizer, and set aside for later use.

[0033] A method for fertilizing rubber forest tortoises in stages includes the following steps: (1) Select a rubber forest with a canopy density of 0.5 in Mengla County, Xishuangbanna, Yunnan Province, and apply well-rotted cow manure at a rate of 2200 kg / mu to plow the forest land to obtain the plowed forest land. (2) At the end of April in spring, the seedlings of the mountain tortoise were planted in the tilled forest land at a density of 1,000 seedlings / mu, with a distance of 1m between the seedlings and the roots of the rubber trees; (3) After the seedlings are planted, seedling fertilizer is applied to the seedlings every 20 days by hole application, twice, with an application rate of 28 kg / mu each time; (4) Apply compound fertilizer every 30 days during the growing season, for a total of 4 applications, with each application amount being 85 kg / mu; apply 600 kg / mu of well-rotted sheep manure once during the dormant period. (5) Apply compound fertilizer 2 every 30 days during the second year’s growing season, 5 times, with an application rate of 95 kg / mu each time; top the vines of the mountain tortoise every 20 days; spray the leaves with a 0.5 wt% humic acid solution every 25 days, 4 times, with an application rate of 50 L / mu each time; apply 500 kg / mu of well-rotted sheep manure once during the dormant period. (6) Apply compound fertilizer 3 every 30 days during the third year of growth, 4 times, with an application rate of 70 kg / mu each time; prune the vines of the mountain tortoise every 20 days, pruning them to 1 meter / strip; spray the leaves with a 0.2 wt% potassium dihydrogen phosphate solution every 25 days, with an application rate of 35 L / mu each time; harvest the tuberous roots of the mountain tortoise during the dormant period.

[0034] Example 2

[0035] Preparation of seedling fertilizer: Mix 20 kg of superphosphate, 5 kg of zinc sulfate, 50 kg of humic acid and 60 kg of well-rotted cow manure evenly, crush them, and granulate them to obtain seedling fertilizer for later use.

[0036] Compound Fertilizer 1: Mix ammonium chloride, superphosphate and potassium chloride evenly in a mass ratio of nitrogen, phosphorus and potassium of 1:1:1 to obtain Compound Fertilizer 1, and set aside.

[0037] Compound Fertilizer 2: Mix ammonium chloride, superphosphate, potassium chloride and sodium silicate evenly in a mass ratio of nitrogen, phosphorus, potassium and silicon of 4:8:2:0.5 to obtain Compound Fertilizer 2, and set aside.

[0038] Compound fertilizer 3: Mix ammonium chloride and superphosphate evenly at a nitrogen-to-phosphorus ratio of 2:1 to obtain compound fertilizer, and set aside.

[0039] A method for fertilizing rubber forest tortoises in stages includes the following steps: (1) Select rubber forest land with a canopy density of 0.5 adjacent to Example 1 and Example 1, apply well-rotted sheep manure at a rate of 2000 kg / mu to plow the forest land to obtain the plowed forest land; (2) At the end of April in spring, the seedlings of the mountain tortoise were planted in the tilled forest land at a density of 800 seedlings / mu, with a distance of 1m between the seedlings and the roots of the rubber trees; (3) After the seedlings are planted, seedling fertilizer is applied to the seedlings every 20 days by hole application, 3 times, with an application amount of 30 kg / mu each time; (4) Apply compound fertilizer every 30 days during the growing season, for a total of 5 applications, with each application amount being 88 kg / mu; apply 600 kg / mu of well-rotted sheep manure once during the dormant period. (5) Apply compound fertilizer 2 every 30 days during the second year’s growing season, 4 times, with an application rate of 90 kg / mu each time; top the vines of the mountain tortoise every 20 days; spray the leaves with a 0.4 wt% humic acid solution every 25 days, 4 times, with an application rate of 52 L / mu each time; apply 500 kg / mu of well-rotted sheep manure once during the dormant period. (6) Apply compound fertilizer 3 every 30 days during the third year of growth, 5 times, with an application rate of 70 kg / mu each time; prune the vines of the mountain tortoise every 20 days, pruning them to 0.8 meters / vine; spray the leaves with a 0.1 wt% potassium dihydrogen phosphate solution every 25 days, with an application rate of 40 L / mu each time; harvest the tuberous roots of the mountain tortoise during the dormant period.

[0040] Example 3

[0041] Preparation of seedling fertilizer: Mix 20 kg of superphosphate, 5 kg of zinc sulfate, 50 kg of humic acid and 60 kg of well-rotted cow manure evenly, crush them, and granulate them to obtain seedling fertilizer for later use.

[0042] Compound Fertilizer 1: Mix ammonium chloride, superphosphate and potassium chloride evenly in a mass ratio of nitrogen, phosphorus and potassium of 1:1:1 to obtain Compound Fertilizer 1, and set aside.

[0043] Compound Fertilizer 2: Mix ammonium chloride, superphosphate, potassium chloride and sodium silicate evenly in a mass ratio of nitrogen, phosphorus, potassium and silicon of 6:6:4:0.5 to obtain Compound Fertilizer 2, and set aside.

[0044] Compound fertilizer 3: Mix ammonium chloride and superphosphate evenly in a mass ratio of nitrogen, phosphorus, potassium and silicon of 4:3 to obtain compound fertilizer, and set aside for later use.

[0045] A method for fertilizing rubber forest tortoises in stages includes the following steps: (1) Select rubber forest land with a canopy density of 0.5 adjacent to Example 1 and Example 1, apply well-rotted chicken manure at a rate of 1900 kg / mu to plow the forest land and obtain the plowed forest land; (2) At the end of April in spring, the seedlings of the mountain tortoise were planted in the tilled forest land at a density of 1,100 seedlings / mu, with a distance of 1m between the seedlings and the roots of the rubber trees; (3) After the seedlings are planted, seedling fertilizer is applied to the seedlings every 20 days by hole application, once, with an application amount of 30 kg / mu each time. (4) Apply compound fertilizer 1 every 30 days during the growing season, for a total of 4 applications, with each application amount being 88 kg / mu; apply 600 kg / mu of well-rotted sheep manure once during the dormant period. (5) Apply compound fertilizer 2 every 30 days during the second year’s growing season, 4 times, with an application rate of 93 kg / mu each time; top the vines of the mountain tortoise every 20 days; spray the leaves with a 0.3 wt% humic acid solution every 25 days, 4 times, with an application rate of 48 L / mu each time; apply 500 kg / mu of well-rotted sheep manure once during the dormant period. (6) Apply compound fertilizer 3 every 30 days during the third year of growth, 5 times, with an application amount of 68 kg / mu each time; prune the vines of the mountain tortoise every 20 days, pruning them to 1.1 meters / vine; spray the leaves with a 0.2 wt% potassium dihydrogen phosphate solution every 25 days, with an application amount of 40 L / mu each time; harvest the tuberous roots of the mountain tortoise during the dormant period.

[0046] Comparative Example 1

[0047] Compound fertilizer: Mix ammonium chloride, superphosphate and potassium chloride in a mass ratio of nitrogen, phosphorus and potassium of 1:1:1 to obtain compound fertilizer, and set aside for later use.

[0048] A method for routine fertilization of tortoises found in rubber plantations includes the following steps: (1) Select rubber forest land with a canopy density of 0.5 adjacent to Example 1 and Example 1, apply well-rotted cow manure at a rate of 2200 kg / mu to plow the forest land and obtain the plowed forest land; (2) At the end of April in spring, the seedlings of the mountain tortoise were planted in the tilled forest land at a density of 1,000 seedlings / mu, with a distance of 1m between the seedlings and the roots of the rubber trees; (3) After the seedlings are planted, compound fertilizer is applied to the seedlings by hole application every 20 days, twice, with an application rate of 28 kg / mu each time; (4) Apply compound fertilizer every 30 days during the growing season, 4 times, with an application rate of 85 kg / mu each time; apply 600 kg / mu of well-rotted sheep manure once during the dormant period. (5) Apply compound fertilizer every 30 days during the second year’s growing season, five times, with each application amount being 95 kg / mu; apply 500 kg / mu of well-rotted sheep manure once during the dormant period; (6) Apply compound fertilizer every 30 days during the third year of growth, 4 times, with an application rate of 70 kg / mu each time; harvest the tuberous roots of the mountain tortoise during the dormant period.

[0049] Comparative Example 2: Compound fertilizer: Mix ammonium chloride, superphosphate and potassium chloride in a mass ratio of nitrogen, phosphorus and potassium of 1:1:1 to obtain compound fertilizer, and set aside for later use.

[0050] A method for routine fertilization of tortoises found in rubber plantations includes the following steps: (1) Select rubber forest land with a canopy density of 0.5 near Example 1 and Example 1, and apply well-rotted cow manure at a rate of 2200 kg / mu to plow the forest land to obtain plowed forest land; (2) At the end of April in spring, the seedlings of the mountain tortoise were planted in the tilled forest land at a density of 1,000 seedlings / mu, with a distance of 1m between the seedlings and the roots of the rubber trees; (3) After the seedlings are planted, apply 56 kg of compound fertilizer per mu at one time using the hole application method; (4) Apply 340 kg of compound fertilizer per mu during the current growing season; apply 600 kg of well-rotted sheep manure per mu during the dormant period. (5) Apply 380 kg of compound fertilizer per mu during the second year's growing season; apply 500 kg of well-rotted sheep manure per mu during the dormant period. (6) Apply 280 kg of compound fertilizer per mu during the third year of growth; harvest the tuberous roots of the mountain turtle during the dormant period.

[0051] Comparative Example 3

[0052] Preparation of seedling fertilizer: Mix 20 kg of superphosphate, 5 kg of zinc sulfate, 50 kg of humic acid and 60 kg of well-rotted cow manure evenly, crush them, and granulate them to obtain seedling fertilizer for later use.

[0053] Compound Fertilizer 1: Mix ammonium chloride, superphosphate and potassium chloride evenly in a mass ratio of nitrogen, phosphorus and potassium of 1:1:1 to obtain Compound Fertilizer 1, and set aside.

[0054] Compound Fertilizer 2: Mix ammonium chloride, superphosphate, potassium chloride and sodium silicate evenly in a mass ratio of nitrogen, phosphorus, potassium and silicon of 5:7:3:0.5 to obtain Compound Fertilizer 2, and set aside.

[0055] Compound fertilizer 3: Mix ammonium chloride and superphosphate evenly in a nitrogen-to-phosphorus ratio of 3:2 to obtain compound fertilizer, and set aside for later use.

[0056] A method for fertilizing rubber forest tortoises in stages includes the following steps: (1) Select a rubber forest with a canopy density of 0.5 in Mengla County, Xishuangbanna, Yunnan Province, and apply well-rotted cow manure at a rate of 2200 kg / mu to plow the forest land to obtain the plowed forest land. (2) At the end of April in spring, the seedlings of the mountain tortoise were planted in the tilled forest land at a density of 1,000 seedlings / mu, with a distance of 1m between the seedlings and the roots of the rubber trees; (3) After the seedlings are planted, seedling fertilizer is applied to the seedlings every 20 days by hole application, twice, with an application rate of 28 kg / mu each time; (4) Apply compound fertilizer every 30 days during the growing season, for a total of 4 applications, with each application amount being 85 kg / mu; apply 600 kg / mu of well-rotted sheep manure once during the dormant period. (5) Apply compound fertilizer 2 every 30 days during the second year’s growing season, for a total of 5 applications, with each application amount being 95 kg / mu; apply 500 kg / mu of well-rotted sheep manure once during the dormant period. (6) Apply compound fertilizer 3 every 30 days during the third year of growth, for a total of 4 applications, with each application amount being 70 kg / mu; harvest the tuberous roots of the mountain tortoise during the dormant period.

[0057] Experimental Example 1

[0058] Twenty tubers of *Solanum tuberosum* from Example 1 and Comparative Examples 1-3 were collected and weighed. The average weight was calculated, and the weights of the tubers from different methods were compared. The results are shown in Table 1.

[0059] Table 1 shows the weight of the mountain tortoise tuber obtained by different methods.

[0060] As shown in Table 1, the average weight of the mountain tortoise tubers obtained by different schemes is not the same. The average weight of the mountain tortoise tubers in Example 1 is significantly higher than that in Comparative Examples 1 to 3. The phased fertilization method of this application meets the growth needs of mountain tortoises at different stages, increases the accumulation of mountain tortoise tuber biomass, and accelerates the growth of mountain tortoise tubers.

[0061] Experiment Example 2

[0062] The main components of *Trionyx sinensis* are alkaloids, possessing sedative, analgesic, and antiarrhythmic pharmacological activities. Its main component, Crebanine, exhibits good antiarrhythmic activity and is a key raw material for extracting 1-Tetrahgdropalmatine (Rotundina), showing promising development and application potential. Clinical trials have shown a direct correlation between the total alkaloid content of *Trionyx sinensis* and its therapeutic effects. Alkaloids were extracted from the tubers of *Trionyx sinensis* in Example 1 and Comparative Examples 1-3, and the effective component content of *Trionyx sinensis* tubers cultivated under different fertilization schemes was compared.

[0063] The extraction method of alkaloids from mountain tortoises is as follows: (1) Sample processing Wash the collected tuberous roots of the mountain tortoise to remove mud and sand, scrape off the rough outer skin, slice them, and air-dry them in a well-ventilated indoor area. Then, dry them in a constant temperature forced-air drying oven at 60℃ for 4 hours. Grind them using a plant pulverizer and pass them through an 80-mesh sieve for later use. (2) Extraction method Weigh 40g of mountain tortoise powder and place it in a 500mL beaker. Add 480mL of 75% ethanol and soak at room temperature for 1 hour. First, extract by ultrasonication for 30 minutes (ultrasonic power 1500W, frequency 40kHz), then heat under reflux for 2 hours and filter. Add 200mL of 75% ethanol to the remaining residue and heat under reflux for 1 hour, then filter. Combine the two filtrates, concentrate the filtrate under reduced pressure using a rotary evaporator to a paste, cool, dissolve in 3% HCl solution, and filter to remove insoluble matter.

[0064] The tuberous roots of *Scutellaria barbata* from Examples 1 and Comparative Examples 1-3 were pulverized to obtain corresponding powders. The alkaloid content in the tuberous roots of *Scutellaria barbata* from Examples 1 and Comparative Examples 1-3 was extracted using the method described above. The results are shown in Table 2.

[0065] Table 2. Alkaloid content in the tuberous roots of *Trionyx sinensis* from Example 1 and Comparative Examples 1-3.

[0066] As shown in Table 2, the alkaloid content in the tuberous roots of *Trionyx sinensis* prepared in Example 1 was the highest, at 63.371 mg / g. This indicates that the phased fertilization of *Trionyx sinensis* in this application not only increases the yield of *Trionyx sinensis*, but also increases the alkaloid content in the tuberous roots, thereby enhancing its medicinal value.

[0067] As can be seen from the above embodiments, the present invention provides a staged fertilization method for *Triplophysa gracilis* under rubber plantations. This invention provides a staged fertilization method for the three-year growth of *Triplophysa gracilis*. In the first year, fertilization during the seedling stage ensures seedling growth. During the growing season, compound fertilizer is applied to ensure the nutritional needs of *Triplophysa gracilis*, promoting balanced development of tubers and vines. In the second year, the compound fertilizer applied is supplemented with silicon to promote root growth. The element ratio and dosage of the compound fertilizer are also modified. Combined with pruning of the above-ground vines and foliar spraying, photosynthesis is increased, further accelerating the accumulation of nutrients in the roots. In the third year, above-ground pruning and foliar spraying promote the accelerated transfer of nutrients from the above-ground parts to the underground. Through this three-year staged fertilization method, rapid growth of *Triplophysa gracilis* tubers and nutrient accumulation are achieved, improving the yield and quality of *Triplophysa gracilis*.

[0068] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A method for staged fertilization of tortoises in rubber forests, characterized in that, The steps include the following: (1) Select rubber forest land with a canopy density of 0.4~0.6, apply base fertilizer and plow the forest land to obtain plowed forest land; (2) In spring, the seedlings of the mountain tortoise are planted in the tilled forest land at a density of 900-1100 seedlings / mu, with the distance between the seedlings and the roots of the rubber trees being 0.5-1.5m. (3) Apply seedling fertilizer every 15 to 25 days after planting, 1 to 3 times, with an application rate of 25 to 30 kg / mu each time; (4) Apply compound fertilizer every 25-35 days during the growing season, 3-5 times; apply 500-700 kg / mu of well-rotted sheep manure once during the dormant period; (5) Apply compound fertilizer 2 every 25-35 days during the second year’s growing season, 4-6 times; top the vines of the mountain tortoise every 15-25 days; spray the leaves with humic acid solution every 20-30 days, 3-5 times; apply 400-600 kg / mu of decomposed sheep manure once during the dormant period. (6) Apply compound fertilizer 3 every 25-35 days during the third year of growth, apply 3-5 times, prune the vines of the mountain tortoise every 15-25 days, spray the leaves with potassium dihydrogen phosphate solution every 20-30 days, and harvest the tubers of the mountain tortoise during the dormant period. The seedling fertilizer mentioned in step (3) comprises the following components in parts by weight: 3-5 parts superphosphate, 0.5-1.5 parts zinc sulfate, 9-11 parts humic acid, and 11-13 parts well-rotted cow manure; The compound fertilizer 1 mentioned in step (4) includes nitrogen, phosphorus and potassium elements, and the mass ratio of nitrogen, phosphorus and potassium elements is 1~2:1~2:1~2; the application rate of compound fertilizer 1 each time is 80~90 kg / mu; The compound fertilizer 2 mentioned in step (5) includes nitrogen, phosphorus, potassium and silicon elements, and the mass ratio of nitrogen, phosphorus, potassium and silicon elements is 4~6:6~8:2~4:0.4~0.6; the application rate of compound fertilizer 2 each time is 90~100 kg / mu; The concentration of the humic acid aqueous solution in step (5) is 0.4~0.6wt%, and the spraying amount for each application is 45~55L / mu; The compound fertilizer 3 mentioned in step (6) includes nitrogen and phosphorus elements, and the mass ratio of nitrogen to phosphorus elements is 2~4:1~3; the application rate of the compound fertilizer 3 each time is 60~80 kg / mu; The concentration of the potassium dihydrogen phosphate aqueous solution in step (6) is 0.1~0.3wt%, and the spraying amount for each application is 30~40L / mu.

2. The method according to claim 1, characterized in that, The base fertilizer mentioned in step (1) is well-rotted animal manure; The amount of base fertilizer used is 1800~2600 kg / mu.

3. The method according to claim 1, characterized in that, The pruning method described in step (6) is to prune the vines of the mountain tortoise to 0.5~1.5 meters / s.

4. The application of the method according to any one of claims 1 to 3 in the breeding of high-quality mountain turtles.

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