A method for improving survival rate of induced spawning of parent fish of pallasia pallasia by precise regulation

Abstract

The application provides a method for improving the survival rate of induced spawning of Pseudosciaena crocea parent fish of the Lanyu population through precise regulation, and belongs to the technical field of marine fish breeding. The method comprises the following steps: S1, pre-production cultivation and regulation stage, bait nutrition matching, and cultivation environment regulation; S2, environment transition stage, gradient environment adaptation, construction of cultivation ponds, transition ponds, and induced spawning ponds, three-stage transition system; batch transfer, parent fish are first transferred from the cultivation ponds to the transition ponds; S3, precise induction stage, parent fish are grouped; S4, post-production care stage, after spawning, the parent fish are immediately separated and transferred to the care ponds. Through stage-by-stage regulation of the cultivation environment, customized hormone programs, refined post-production care, and targeted nutritional reinforcement, physical damage and post-production infection during the induction process are reduced, the post-production recovery speed and reusability of the parent fish are improved, and the induced spawning survival rate is improved.

Description

Technical Field

[0001] This application relates to the field of marine fish breeding, and more specifically, to a method for precisely controlling the spawning survival rate of large yellow croaker broodstock. Background Technology

[0002] Currently, the large yellow croaker from Naozhou often lives in groups and mainly feeds on crustaceans such as shrimp and crabs, or small fish. Due to their long-term exposure to warm environments, the Naozhou large yellow croaker has developed better heat tolerance and immune adaptability. Inducing spawning in parent fish is a crucial step in the artificial breeding of large yellow croaker; traditional spawning induction methods have the following drawbacks:

[0003] 1. Strong environmental stress: The temperature, salinity, and flow of the spawning tank are poorly regulated. When the broodstock are transferred from the rearing tank to the spawning tank, the environment changes abruptly, and the stress response is severe (such as swimming wildly and colliding with the tank wall), resulting in damage to the body surface and decreased immunity.

[0004] 2. Inappropriate use of hormones: Single hormones (such as human chorionic gonadotropin HCG) or fixed dose combinations are often used without being adjusted according to the differences in the weight and gonadal maturity of the broodstock. This can easily lead to excessive hormones causing metabolic disorders, or insufficient doses that prevent normal spawning. Furthermore, the mortality rate of broodstock after spawning can be as high as 30%-40%.

[0005] 3. Lack of postpartum care: After spawning, the parent fish were directly returned to the ordinary breeding pond without special care for the weak and susceptible to infection characteristics of the parent fish after spawning. As a result, wound infections (such as oviposition hole inflammation) and secondary diseases (such as bacterial sepsis) were common.

[0006] 4. Single nutritional intake: During the early stages of induced spawning, broodstock are fed only frozen food, which lacks key nutrients that promote gonadal development and resist stress. As a result, the broodstock are in poor condition and cannot tolerate the induced spawning process.

[0007] The aforementioned problems result in low survival rates of broodstock large yellow croaker after induced spawning and a limited number of reuses, thus restricting the efficiency of large-scale breeding. Summary of the Invention

[0008] To overcome the above shortcomings, this application provides a method for improving the survival rate of broodstock spawning through precise regulation, aiming to improve the problems of strong stress, high damage rate and low survival rate of broodstock during traditional spawning induction processes.

[0009] This application provides a method for precisely controlling the spawning and survival rate improvement of broodstock large yellow croaker, including the following steps:

[0010] S1, Prenatal care and regulation stage, 30-45 days before labor induction;

[0011] The feed is nutritionally formulated by combining basic feed with functional additives; the functional additives include anti-stress agents, gonadal development promoters, and immune enhancers.

[0012] The cultivation environment is controlled by maintaining the water temperature in the cultivation tank at 18-20℃, the salinity at 28-31‰, the dissolved oxygen at ≥6mg / L, the daily light exposure at 12h, and the light intensity at 500-800lux, and keeping the environmental parameters stable.

[0013] S2, the environmental transition phase, 2-3 days before labor induction;

[0014] Gradient environment adaptation: a three-level transition system is constructed, consisting of a rearing tank, a transition tank, and a spawning tank. The environmental parameters of the transition tank are consistent with those of the rearing tank, while the initial parameters of the spawning tank are controlled to be within the range of: water temperature ±0.5℃, salinity ±2‰, and dissolved oxygen ±0.4mg / L.

[0015] The fish were transferred in batches. The parent fish were first transferred from the rearing pond to the transition pond and allowed to adapt for 24 hours. Then, the parent fish were transferred to the spawning induction pond. The parameters of the spawning induction pond were gradually adjusted to the target value at a rate of one parameter every 6 hours.

[0016] S3, Precision Labor Induction Stage

[0017] The broodstock were divided into groups, and each group was placed in a separate spawning tank. A combination of luteinizing hormone-releasing hormone analogs and dopamine antagonists was used to administer differential hormone injections to each group.

[0018] Monitoring of the spawning process: After injection, maintain the stability of the spawning tank environment and observe the activity status of the parent fish every 2 hours. When the parent fish exhibit chasing and pairing behaviors, reduce the water flow speed to 0.06-0.1 m / s to prepare for spawning collection. Personnel are prohibited from approaching the edge of the tank during the spawning process.

[0019] S4. Postpartum care stage, 1-15 days postpartum;

[0020] After spawning, the parent fish are immediately separated and transferred to a care tank with environmental parameters consistent with those of the spawning induction tank to avoid secondary stress. They are then bathed in a 3‰ saline solution for 10-15 minutes to disinfect any wounds on their bodies. At the same time, erythromycin ointment is applied to any parent fish with red and swollen spawning holes to prevent infection.

[0021] In a preferred embodiment of the present invention, the basic bait includes fresh sand eels and formulated feed, wherein the fresh sand eels and the formulated feed are mixed in a 7:3 ratio.

[0022] In a preferred embodiment of the present invention, the frozen sand eel is chopped into 2-3cm pieces, and the feed includes crude protein and crude fat, wherein the crude protein is ≥45% and the crude fat is ≥12%.

[0023] In a preferred embodiment of the present invention, the anti-stress agent includes vitamin C and taurine, which enhances the stress resistance of broodstock.

[0024] The gonadal development promoter includes arachidonic acid and zinc, which promote oocyte maturation.

[0025] The immune enhancer includes yeast cell walls, which enhance the immunity of the parent fish.

[0026] In a preferred embodiment of the present invention, the daily dosage of the functional additives is as follows: vitamin C at a feed ratio of 500 mg / kg, taurine at a feed ratio of 300 mg / kg, arachidonic acid at a feed ratio of 100 mg / kg, zinc at a feed ratio of 50 mg / kg, and yeast cell wall at a feed ratio of 200 mg / kg.

[0027] In a preferred embodiment of the present invention, the water temperature in the cultivation tank is maintained by a constant temperature heating rod linked with a chiller; the salinity of the cultivation tank is monitored in real time by a salinity meter and adjusted with fresh water or sea salt; dissolved oxygen is aerated by both an aeration disc and an aerator; and the light intensity is achieved by using LED dimmable lamps to simulate the natural light rhythm.

[0028] In a preferred embodiment of the present invention, the target values ​​for adjusting the parameters of the spawning tank include a water temperature of 21-22℃, a salinity of 30-31‰, dissolved oxygen ≥7mg / L, and a water flow velocity of 0.1-0.2m / s; the density of the transition tank is ≤5 spawners / m³. 3 The induced labor tank has an area of ​​10-15m². 2 The water is 1.5m deep.

[0029] In a preferred embodiment of the present invention, during the precise spawning induction stage, the broodstock are grouped according to their weight and gonadal maturity; they are divided into three groups based on weight: 1.0-1.5kg, 1.5-2.0kg, and over 2.0kg; gonadal maturity is determined by ultrasound, with oocytes having a diameter ≥450μm considered mature.

[0030] Hormone injection dosage adjusted according to body weight:

[0031] 1.0-1.5kg group: LHRH-A25μg / kg+DOM2mg / kg;

[0032] 1.5-2.0kg group: LHRH-A 24μg / kg + DOM 1.5mg / kg;

[0033] Group over 2.0kg: LHRH-A 23μg / kg + DOM 1mg / kg;

[0034] The injection method is intramuscular injection at the base of the pectoral fin, with an injection depth of 0.5-1cm and an injection speed of ≤0.2mL / s, to avoid damage to internal organs.

[0035] In a preferred embodiment of the present invention, during the postpartum care stage, within 1-3 days postpartum, the fish are fed easily digestible copepod larvae and cooked fish paste, with probiotics added at 100 cfu / g of feed; fed twice daily at 1%-2% of the parent fish's body weight; from 4-7 days postpartum, the fish are gradually transitioned to pre-partum fortified feed, with the feeding amount increased to 3%-4%; from 8-15 days postpartum, the normal feeding amount is restored to 5%.

[0036] In a preferred embodiment of the present invention, during the postpartum care stage, the water temperature in the care tank is maintained at 20-21°C, dissolved oxygen ≥7mg / L, and 1 / 3 of the water is replaced daily with ultraviolet-disinfected seawater. The body surface and feeding status of the broodstock are observed daily; broodstock with poor vitality are fed separately and supplemented with vitamin B. 12 Promotes recovery, Vitamin B 12 The dosage was 200 μg / kg of feed; 15 days after spawning, the weight recovery rate and gonadal degeneration of the parent fish were tested, and those that passed were transferred to ordinary rearing ponds.

[0037] Beneficial effects: This application provides a method for improving the survival rate of broodstock of large yellow croaker with precise regulation and spawning. By regulating the breeding environment in stages, customizing hormone programs, providing refined postpartum care and targeted nutritional enhancement, the method reduces environmental and hormonal stress on broodstock; reduces physical damage and postpartum infection during spawning; and improves the postpartum recovery speed and reusability of broodstock, thereby increasing the survival rate of spawning.

[0038] 1. A three-level environmental transition system is adopted, consisting of a rearing tank, a transition tank and a spawning tank. The initial parameter differences between the transition tank and the spawning tank are limited, and the stress response is significantly reduced by adjusting the parameters in a gradient adaptation manner. The fine transition improves the adaptability of the broodstock.

[0039] 2. Differential hormone injection is adopted. Based on the weight of the parent fish (1.0-1.5kg, 1.5-2.0kg, and over 2.0kg) and the maturity of the gonads (oocyte diameter ≥450μm as detected by ultrasound), a decreasing hormone dosage is designed, and a precise control differential logic of "the greater the weight, the lower the dosage" is adopted.

[0040] 3. A detailed design for phased postpartum care over 15 days extends the care period to 15 days, with phased feed design using copepod larvae, cooked fish paste, pre-partum feed, and added probiotics and vitamin B. 12 This meticulous care, which monitors weight recovery rate and gonadal degeneration, greatly improves postpartum recovery and survival rates. Detailed Implementation

[0041] In this invention, unless otherwise explicitly specified and limited, the terms "installation," "connection," "linking," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances.

[0042] In this invention, unless otherwise explicitly specified and limited, "above" or "below" the second feature can include direct contact between the first and second features, or contact between the first and second features through another feature between them. Furthermore, "above," "over," and "on top" of the second feature includes the first feature directly above or diagonally above the second feature, or simply indicates that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature includes the first feature directly below or diagonally below the second feature, or simply indicates that the first feature is at a lower horizontal level than the second feature.

[0043] The technical solutions in the embodiments of this application will be described below with reference to the embodiments of this application.

[0044] This invention provides a method for precisely controlling the spawning and survival rate improvement of broodstock large yellow croaker from the Naozhou group, comprising the following steps:

[0045] S1, Prenatal care and regulation stage, 30-45 days before labor induction;

[0046] The feed is nutritionally formulated by combining basic feed with functional additives; the functional additives include anti-stress agents, gonadal development promoters, and immune enhancers.

[0047] The rearing environment is controlled by maintaining the water temperature in the rearing pond at 18-20℃, the salinity at 28-31‰, the dissolved oxygen at ≥6mg / L, the light exposure at 12 hours per day, and the light intensity at 500-800 lux, and keeping the environmental parameters stable. In the pre-birth stage, "anti-stress agents, gonadal development promoters and immune enhancers are combined in specific dosages with the basic feed to form a precise nutrition program."

[0048] S2, the environmental transition phase, 2-3 days before labor induction;

[0049] Gradient environment adaptation: a three-level transition system is constructed, consisting of a rearing tank, a transition tank, and a spawning tank. The environmental parameters of the transition tank are consistent with those of the rearing tank, while the initial parameters of the spawning tank are controlled to be within the range of: water temperature ±0.5℃, salinity ±2‰, and dissolved oxygen ±0.4mg / L.

[0050] The fish were transferred in batches. The parent fish were first transferred from the rearing pond to the transition pond and allowed to adapt for 24 hours. Then, the parent fish were transferred to the spawning induction pond. The parameters of the spawning induction pond were gradually adjusted to the target value at a rate of one parameter every 6 hours.

[0051] S3, Precision Labor Induction Stage

[0052] The broodstock were divided into groups, and each group was placed in a separate spawning tank. A combination of luteinizing hormone-releasing hormone analogs and dopamine antagonists was used to administer differential hormone injections to each group.

[0053] Monitoring of the spawning process: After injection, maintain the stability of the spawning tank environment and observe the activity status of the parent fish every 2 hours. When the parent fish exhibit chasing and pairing behaviors, reduce the water flow speed to 0.06-0.1 m / s to prepare for spawning collection. Personnel are prohibited from approaching the edge of the tank during the spawning process.

[0054] S4. Postpartum care stage, 1-15 days postpartum;

[0055] After spawning, the parent fish are immediately separated and transferred to a care tank with environmental parameters consistent with those of the spawning induction tank to avoid secondary stress. They are then bathed in a 3‰ saline solution for 10-15 minutes to disinfect any wounds on their bodies. At the same time, erythromycin ointment is applied to any parent fish with red and swollen spawning holes to prevent infection.

[0056] Through a four-stage closed-loop program encompassing prenatal care and regulation, environmental transition, precise labor induction, and postpartum care, the parameters of each stage are integrated into a synergistic regulatory logic. The program's stage division and collaborative design enable multi-element synergistic regulation.

[0057] After reducing stress through a three-stage transition, hormones are injected according to individual differences in physical condition, avoiding the loss of broodstock caused by "uniform hormone injection under high stress" in existing technologies; achieving improved survival rate, reduced postpartum infection rate, reduced stress index, reduced surface damage rate; and increased number of repeat induced spawning cycles.

[0058] 1. Survival rate is significantly improved. Through three-level environmental transition, customized hormone program and 15 days of special care, the survival rate of broodstock spawning is increased from the traditional 60%-70% to more than 90%, the postpartum infection rate is reduced to less than 5%, and the number of spawning inductions can be increased from 1-2 times to 3-4 times.

[0059] 2. Gradient environment adaptation and gentle operation reduce the stress index during the transfer of parent fish, minimizing stress response and greatly reducing the rate of surface damage.

[0060] 3. The spawning efficiency and quality are both taken into account. Different hormone dosages can increase the spawning rate of broodstock to over 85% and the fertilization rate of eggs to ≥80% (compared to about 70% with traditional methods). Furthermore, the broodstock recover quickly after spawning, shortening the interval between the next spawning induction (from 60 days to 45 days).

[0061] 4. Highly operable, all steps rely on conventional breeding equipment (constant temperature heating rod, B-ultrasound, salinity meter, etc.), no special instruments are required, feed additives are readily available, suitable for large-scale application in small and medium-sized breeding farms, reducing the cost of broodstock per 10,000 fry by 30%-40%.

[0062] In a specific embodiment of the present invention, the basic bait includes fresh jade shark and formulated feed, wherein the fresh jade shark and the formulated feed are mixed in a ratio of 7:3.

[0063] In a specific embodiment of the present invention, the frozen jade fish is chopped into 2-3cm pieces, and the feed includes crude protein and crude fat, wherein the crude protein is ≥45% and the crude fat is ≥12%.

[0064] In a specific embodiment of the present invention, the anti-stress agent includes vitamin C and taurine, which enhance the stress resistance of broodstock.

[0065] The gonadal development promoter includes arachidonic acid and zinc, which promote oocyte maturation.

[0066] The immune enhancer includes yeast cell walls, which enhance the immunity of the parent fish.

[0067] In a specific embodiment of the present invention, the daily dosage of the functional additives is as follows: vitamin C at a feed ratio of 500 mg / kg, taurine at a feed ratio of 300 mg / kg, arachidonic acid at a feed ratio of 100 mg / kg, zinc at a feed ratio of 50 mg / kg, and yeast cell wall at a feed ratio of 200 mg / kg.

[0068] In a specific embodiment of the present invention, the water temperature in the cultivation tank is maintained by a constant temperature heating rod linked with a chiller; the salinity of the cultivation tank is monitored in real time by a salinity meter and adjusted with fresh water or sea salt; dissolved oxygen is aerated by both an aeration disc and an aerator; and the light intensity is achieved by using LED dimmable lamps to simulate the natural light rhythm.

[0069] In a specific embodiment of the present invention, the target values ​​for adjusting the parameters of the spawning induction tank include a water temperature of 21-22℃, a salinity of 30-31‰, dissolved oxygen ≥7mg / L, and a water flow velocity of 0.1-0.2m / s; the density of the transition tank is ≤5 spawners / m³. 3 The induced labor tank has an area of ​​10-15m². 2 The water is 1.5m deep.

[0070] In a specific embodiment of the present invention, during the precise spawning induction stage, the broodstock are grouped according to their weight and gonadal maturity; they are divided into three groups based on weight: 1.0-1.5kg, 1.5-2.0kg, and over 2.0kg; gonadal maturity is determined by ultrasound, with oocytes having a diameter ≥450μm considered mature.

[0071] Hormone injection dosage adjusted according to body weight:

[0072] 1.0-1.5kg group: LHRH-A25μg / kg+DOM2mg / kg;

[0073] 1.5-2.0kg group: LHRH-A 24μg / kg + DOM 1.5mg / kg;

[0074] Group over 2.0kg: LHRH-A 23μg / kg + DOM 1mg / kg;

[0075] The injection method is intramuscular injection at the base of the pectoral fin, with an injection depth of 0.5-1cm and an injection speed of ≤0.2mL / s, to avoid damage to internal organs.

[0076] In a specific embodiment of the present invention, during the postpartum care stage, within 1-3 days postpartum, feed easily digestible copepod larvae and cooked fish paste, with probiotics added at 100 cfu / g of feed; feed twice daily at a rate of 1%-2% of the parent fish's body weight; from 4-7 days postpartum, gradually transition to pre-partum fortified feed, increasing the feed rate to 3%-4%; from 8-15 days postpartum, restore the normal feed rate to 5%.

[0077] In a specific embodiment of the present invention, during the postpartum care stage, the water temperature in the care tank is maintained at 20-21℃, dissolved oxygen is ≥7mg / L, and 1 / 3 of the water is replaced daily with seawater disinfected by ultraviolet light. The body surface and feeding status of the parent fish are observed daily. For parent fish with poor vitality, they are fed separately and vitamin B12 is added to promote recovery. The dosage of vitamin B12 is 200μg / kg of feed. 15 days after spawning, the weight recovery rate and the degree of gonadal degeneration of the parent fish are tested. After passing the test, they are transferred to a regular rearing tank.

[0078] In practical implementation, the following points should be noted:

[0079] When selecting broodstock, prioritize those with intact bodies, strong vitality, and no history of disease, and avoid using injured or emaciated individuals (weighing less than 1.0kg).

[0080] Equipment calibration: Before inducing labor, equipment such as water temperature controller, salinity meter, and ultrasound should be calibrated to ensure accurate parameter monitoring (water temperature error ≤ 0.3℃, salinity error ≤ 1‰).

[0081] Hormone storage: LHRH-A2 and DOM should be stored in the dark and refrigerated (2-8℃). Before use, allow them to warm to room temperature for 30 minutes and avoid repeated freeze-thaw cycles.

[0082] In case of emergency, if the parent fish show severe stress (such as surfacing or not feeding), immediately apply vitamin C solution (100 mg / L) and reduce the water flow rate. If necessary, isolate and care for them separately.

[0083] The above description is merely an embodiment of this application and is not intended to limit the scope of protection of this application. Various modifications and variations can be made to this application by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this application should be included within the scope of protection of this application.

Claims

1. A method for precisely controlling and improving the survival rate of broodstock of large yellow croaker from the Naozhou group, characterized in that, The steps include the following: S1, Prenatal care and regulation stage, 30-45 days before labor induction; The feed is nutritionally formulated by combining basic feed with functional additives; the functional additives include anti-stress agents, gonadal development promoters, and immune enhancers. The cultivation environment is controlled by maintaining the water temperature in the cultivation tank at 18-20℃, the salinity at 28-31‰, the dissolved oxygen at ≥6mg / L, the daily light exposure at 12h, and the light intensity at 500-800lux, and keeping the environmental parameters stable. S2, the environmental transition phase, 2-3 days before labor induction; Gradient environment adaptation: a three-level transition system is constructed, consisting of a rearing tank, a transition tank, and a spawning tank. The environmental parameters of the transition tank are consistent with those of the rearing tank, while the initial parameters of the spawning tank are controlled to be within the range of: water temperature ±0.5℃, salinity ±2‰, and dissolved oxygen ±0.4mg / L. The fish were transferred in batches. The parent fish were first transferred from the rearing pond to the transition pond and allowed to adapt for 24 hours. Then, the parent fish were transferred to the spawning induction pond. The parameters of the spawning induction pond were gradually adjusted to the target value at a rate of one parameter every 6 hours. S3, Precision Labor Induction Stage The parent fish were divided into groups, and each group of parent fish was placed in a separate spawning tank. A combination of luteinizing hormone-releasing hormone analogues and dopamine antagonists was used to administer differential hormone injections to each group; Monitoring of the spawning process: After injection, maintain the stability of the spawning tank environment and observe the activity status of the parent fish every 2 hours. When the parent fish exhibit chasing and pairing behaviors, reduce the water flow speed to 0.06-0.1 m / s to prepare for spawning collection. Personnel are prohibited from approaching the edge of the tank during the spawning process. S4. Postpartum care stage, 1-15 days postpartum; After spawning, the parent fish are immediately separated and transferred to the care tank with environmental parameters consistent with those of the spawning induction tank to avoid secondary stress. They are then bathed in a 3‰ saline solution for 10-15 minutes to disinfect any wounds on their bodies. At the same time, erythromycin ointment is applied to parent fish with red and swollen spawning holes to prevent infection. The anti-stress agents include vitamin C and taurine, which enhance the stress resistance of broodstock. The gonadal development promoter includes arachidonic acid and zinc, which promote oocyte maturation. The immune enhancer includes yeast cell walls, which enhance the immunity of the parent fish; During the precise spawning induction phase, the broodstock were grouped according to their weight and gonadal maturity. Based on weight, they were divided into three groups: 1.0-1.5kg, 1.5-2.0kg, and over 2.0kg. Gonadal maturity was determined by ultrasound, with oocytes ≥450μm in diameter considered mature. Hormone injection dosage adjusted according to body weight: 1.0-1.5kg group: ; 1.5-2.0kg group: ; Groups weighing 2.0kg and above: ; The injection method is intramuscular injection at the base of the pectoral fin, with an injection depth of 0.5-1cm and an injection speed of ≤0.2mL / s, to avoid damage to internal organs; During the postpartum care phase, within 1-3 days postpartum, feed easily digestible copepod larvae and cooked fish paste, with probiotics added at 100 cfu / g of feed; feed twice daily at 1%-2% of the parent fish's body weight; from 4-7 days postpartum, gradually transition to pre-partum fortified feed, increasing the feed amount to 3%-4%; from 8-15 days postpartum, resume the normal feed amount of 5%.

2. The method for precisely controlling the spawning and survival rate improvement of broodstock of large yellow croaker (Cyprinus naanensis) according to claim 1, characterized in that, The basic bait includes fresh sand eels and formulated feed, with the fresh sand eels and formulated feed mixed in a 7:3 ratio.

3. The method for improving the survival rate of broodstock of large yellow croaker (Cyprinus naanensis) through precise regulation according to claim 2, characterized in that, The frozen sand eel is chopped into 2-3cm pieces, and the feed contains crude protein and crude fat, with crude protein ≥45% and crude fat ≥12%.

4. The method for improving the survival rate of broodstock of large yellow croaker (Cyprinus naanensis) through precise regulation according to claim 1, characterized in that, The daily dosage of the functional additives is as follows: Vitamin C at a feed ratio of 500 mg / kg, Taurine at a feed ratio of 300 mg / kg, Arachidonic acid at a feed ratio of 100 mg / kg, Zinc at a feed ratio of 50 mg / kg, and Yeast cell wall at a feed ratio of 200 mg / kg.

5. The method for precisely controlling the spawning and survival rate improvement of broodstock of large yellow croaker (Cyprinus naanensis) according to claim 1, characterized in that, The water temperature in the culture tank is maintained by a constant temperature heating rod linked with a chiller; the salinity of the culture tank is monitored in real time by a salinity meter and adjusted with fresh water or sea salt; dissolved oxygen is aerated by both aeration discs and aerators; and the light intensity is achieved by using dimmable LED lights to simulate the natural light rhythm.

6. The method for improving the survival rate of broodstock of large yellow croaker (Cyprinus naan) through precise regulation according to claim 1, characterized in that, The target values ​​for adjusting the parameters of the spawning tank include a water temperature of 21-22℃, a salinity of 30-31‰, dissolved oxygen ≥7mg / L, and a water flow velocity of 0.1-0.2m / s; the density of the transition tank is ≤5 tails / m³, the area of ​​the spawning tank is 10-15m², and the water depth is 1.5m.

7. The method for improving the survival rate of broodstock of large yellow croaker (Cyprinus naan) through precise regulation according to claim 1, characterized in that, During the postpartum care phase, the water temperature in the care tank was maintained at 20-21℃, and dissolved oxygen was ≥7mg / L. One-third of the water was replaced daily using seawater disinfected with ultraviolet light. The body surface and feeding status of the broodstock were observed daily. Broodstock with poor vitality were fed separately and supplemented with vitamin B. 12 Promotes recovery, Vitamin B 12 The dosage was 200 μg / kg of feed; 15 days after spawning, the weight recovery rate and gonadal degeneration of the parent fish were tested, and those that passed were transferred to ordinary rearing ponds.