A highly effective plant growth regulator containing methopromine and a preparation method thereof

Abstract

The present application relates to the technical field of plant growth regulator, in particular to a kind of efficient plant growth regulator containing methopromine and preparation method thereof: including the following mass percentage of raw material composition: methopromine 5-40%, indole butyric acid potassium salt 0.2-2%, composite extract 1-10%, humectant 2-8%, antifreeze 2-8% and preservative 0.1-0.5%, the rest is water.In the present application, methopromine inhibits gibberellin synthesis key enzyme, reduces gibberellin level promoting cell elongation from the source, effectively controls plant overgrowth, and terpenes, phenols and L-proline in composite extract of spruce needle and alkali bush seed extract, on the one hand, slightly promote the accumulation of stress resistance hormone abscisic acid, form a suppress and promote hormone balance with methopromine control effect, on the other hand, directly stabilize cell membrane structure, maintain osmotic balance, and jointly enhance the tolerance of plant to adversity.

Description

Technical Field

[0001] This invention relates to the field of plant growth regulators, specifically to a highly efficient plant growth regulator containing mepiquat chloride and its preparation method. Background Technology

[0002] Plant growth regulators are chemical or biological agents that can mimic or interfere with endogenous hormone signals in plants and regulate plant growth and development. Mepiquat chloride, a commonly used plant growth retardant, mainly works by inhibiting gibberellin synthesis, controlling excessive plant growth, promoting sturdy plants and well-developed root systems, and improving crop resistance and yield. Currently, mepiquat chloride is widely used in various crops such as cotton and wheat.

[0003] Existing mepiquat chloride plant growth regulators mainly focus on controlling plant height, lacking synergistic promotion of root development, photosynthetic efficiency, stress resistance, and other aspects, making it difficult to achieve a comprehensive improvement in the overall growth quality of crops.

[0004] Based on this, the present invention provides a highly efficient plant growth regulator containing mepiquat chloride and its preparation method. Summary of the Invention

[0005] The purpose of this invention is to provide a highly efficient plant growth regulator containing mepiquat chloride and its preparation method. The plant growth regulator prepared by this invention not only has significant effects in controlling excessive growth, resisting stress, and promoting root growth and seedling development, but also has excellent synergistic effects and yield-increasing and quality-improving properties, effectively enhancing the overall growth quality and final yield of crops.

[0006] To achieve the above objectives, the present invention provides the following technical solution: a highly efficient plant growth regulator containing mepiquat chloride, comprising the following raw material composition by mass percentage: mepiquat chloride 5-40%, potassium indolebutyrate 0.2-2%, compound extract 1-10%, humectant 2-8%, antifreeze 2-8%, and preservative 0.1-0.5%, with the balance being water; The preparation of the compound extract includes the following steps: extracting fir needles, paper mulberry male flowers and Suaeda salsa seeds to obtain corresponding extracts, and mixing the extracts in a 1:1:1 ratio to complete the preparation of the compound extract.

[0007] Preferably, the extraction and processing method of fir needles is as follows: dried fir needles are selected, pulverized, and passed through a 40-mesh sieve to obtain fir powder. The fir powder is placed in a multifunctional hot reflux extraction tank equipped with stirring and jacket heating. A buffer solution is added, and the mixture is stirred at 200-300 r / min for 10-20 min. Then, a compound enzyme preparation is added, and the temperature is set at 40-50℃. The mixture is stirred at 150 rpm for 2 h. After the reaction, the temperature is raised to 90℃ and maintained for 10-12 min. Subsequently, hot reflux extraction is performed at 90-95℃ for 1 h. The mixture is then immediately removed and filtered through a 200-300 mesh filter. The filtrate is collected, and the filter residue is washed once with 60℃ hot water. The residue is filtered again and the filtrate is collected. The filtrates from the two filtrations are combined and placed in a vacuum concentration tank. The mixture is concentrated under reduced pressure at -0.09 MPa and 60℃ to a paste to obtain fir needle extract, thus completing the extraction and processing of fir needles.

[0008] Preferably, the compound enzyme preparation is prepared by mixing cellulase and pectinase at a mass ratio of 2:1, the buffer solution is prepared by mixing pH 5.0 citrate-disodium hydrogen phosphate solution and L-proline at a mass ratio of 500:1, the mass ratio of the buffer solution, the compound enzyme preparation and the fir powder is 500-600:15-20:100, and the mass ratio of the filter residue to hot water is 1:(0.8-1.2).

[0009] Preferably, the extraction and processing method of the male flowers of Broussonetia papyrifera is as follows: select male flower powder of Broussonetia papyrifera and pass it through a 30-mesh sieve, add it to an ultrasonic extraction tank, then add the extraction solution, turn on the ultrasonic wave, set the power to 300W, and extract at 50℃ for 30-40 minutes. Then filter it through a 200-300 mesh sieve, collect the filtrate, wash the filter residue once with 50% ethanol, filter again and collect the filtrate, combine the filtrates from the two filtrations, place them in a vacuum concentration tank, and concentrate them under reduced pressure at -0.08MPa vacuum and 65℃ until they become a paste, thus obtaining the extract of male flowers of Broussonetia papyrifera, completing the extraction and processing of male flowers of Broussonetia papyrifera.

[0010] Preferably, the extraction solution is prepared by mixing trehalose, anhydrous ethanol and deionized water in a mass ratio of 1:120:150, the mass ratio of the extraction solution to the male flower powder of Broussonetia papyrifera is 10-20:600, and the mass ratio of 50% ethanol to the filter residue is 1:(0.8-1.2).

[0011] Preferably, the extraction and processing method of Suaeda salsa seeds is as follows: select Suaeda salsa seed powder and pass it through a 50-60 mesh sieve. Add the Suaeda salsa seed powder and reaction solution to a reaction vessel at a ratio of 5-10:400. Stir at 100-200 r / min for 1-1.2 h at 60-70℃. Then, slowly adjust the pH of the system to 6.5-7.0 by adding 1.0 mol / L hydrochloric acid solution dropwise. After adjustment, place the mixture in a centrifuge and centrifuge at 5000-6000 r / min for 15-20 min. Collect the supernatant and place it in a vacuum concentration tank. Concentrate the supernatant under reduced pressure at -0.09 MPa vacuum and 70℃ until it becomes a paste to obtain Suaeda salsa seed extract, thus completing the extraction and processing of Suaeda salsa seeds.

[0012] Preferably, the reaction solution is prepared by dissolving sodium hydroxide and potassium silicate in deionized water at a mass ratio of 8:15-20, wherein the mass ratio of deionized water to sodium hydroxide is 240-260:1.

[0013] Preferably, the composite extract is obtained by adding fir needle leaf extract, mulberry male flower extract and Suaeda salsa seed extract to a reaction vessel and stirring at 50-60℃ for 20-40 minutes.

[0014] Preferably, the wetting agent is one or more of alkylphenol polyoxyethylene ether, alkyl polysaccharide glycoside, betaine, sorbitan oleate, polyoxyethylene 20 sorbitan monooleate, and sodium fatty alcohol polyoxyethylene ether sulfate; the antifreeze agent is one or more of ethylene glycol, glycerol, and urea; and the preservative is one or more of potassium sorbate and Kathon.

[0015] A method for preparing a highly effective plant growth regulator containing mepiquat chloride includes the following steps: Step 1: Add half of the water (by weight percentage), antifreeze, preservative, and wetting agent to the preparation vessel, and stir at 200-400 r / min for 5-15 min to form a uniform mixed mother liquor. Step 2: While stirring, add the potassium indolebutyrate and mepiquat chloride (by mass percentage) to the mixed mother liquor from Step 1, increase the rotation speed to 400-600 r / min, control the material temperature at 40-50℃, and stir for 20-40 min to obtain a transparent solution. Step 3: Add the compound extract of the specified mass percentage to the transparent solution obtained in Step 2, and continue stirring for 20-40 minutes at 40-50℃ and 400-600 r / min. Stop heating, add the remaining water, adjust the speed to 300-500 r / min, and stir for 10-20 minutes to obtain the coarse material. Step 4: Filter the coarse material through a 200-300 mesh filter, collect the filtrate, and complete the preparation of the high-efficiency plant growth regulator.

[0016] Compared with the prior art, the beneficial effects of the present invention are: 1. In this invention, mepiquat reduces the level of gibberellin that promotes cell elongation by inhibiting key enzymes in gibberellin synthesis, effectively controlling excessive plant growth. Furthermore, the terpenes, phenols, and L-proline in the extracts of fir needles and Suaeda salsa seeds slightly promote the accumulation of the stress-resistance hormone abscisic acid, forming a hormonal balance with the growth-controlling effect of mepiquat. On the other hand, they directly stabilize cell membrane structure and maintain osmotic balance, jointly enhancing the plant's tolerance to adversity.

[0017] 2. In this invention, potassium indolebutyrate acts as an exogenous auxin signal, activating the signaling pathway and initiating the genetic program of root development. Simultaneously, the compound extract bidirectionally optimizes this program. Flavonoids in the male flowers of Broussonetia papyrifera inhibit PIN protein-mediated auxin efflux, achieving local enrichment of auxin in the roots, thereby positively regulating and amplifying the initial signal. Meanwhile, monosilicic acid in the extract of Suaeda salsa seeds polymerizes in the cell wall to form a silica gel network, significantly enhancing its mechanical strength through bonding with cell wall polysaccharides, thus constructing a robust endoskeleton for the new root system. Multiple synergistic actions work together to cultivate structurally stable and highly efficient superior roots.

[0018] 3. In this invention, the compound extract directly improves photosynthetic efficiency: fir needle extract provides chlorophyll precursors and photosynthetic protective pigments, while paper mulberry male flower extract supplements the sugars and amino acids required for energy metabolism. At the same time, mepiquat reduces the competitive consumption of photosynthetic products by new branches and leaves by inhibiting vegetative growth, forcing more sucrose to be transported to the spike and grains. Meanwhile, the organic acid components in Suaeda salsa seed extract optimize the energy metabolism of grain cells and enhance the overall storage capacity. The synergistic effect of each component achieves the unity of maximizing photosynthetic production capacity and optimizing product distribution, thereby significantly increasing the number of spikes per acre, the number of grains per spike, and the thousand-grain weight. Attached Figure Description

[0019] Figure 1 The present invention provides a flowchart of a highly efficient plant growth regulator containing mepiquat chloride and its preparation method. Detailed Implementation

[0020] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0021] It should be noted that the raw materials used in the following embodiments are all commercially available.

[0022] Example 1:

[0023] A highly effective plant growth regulator containing mepiquat chloride comprises the following raw materials in weight percentages: 5% mepiquat chloride, 0.2% potassium indolebutyrate, 1% compound extract, 2% humectant, 2% antifreeze, and 0.1% preservative, with the balance being water.

[0024] The preparation of the compound extract includes the following steps: extracting fir needles, paper mulberry male flowers and Suaeda salsa seeds to obtain corresponding extracts, and mixing the extracts in a 1:1:1 ratio to complete the preparation of the compound extract.

[0025] The extraction and processing method of fir needles is as follows: Select dried fir needles, pulverize them, and pass them through a 40-mesh sieve to obtain fir powder. Place the fir powder in a multi-functional hot reflux extraction tank equipped with stirring and jacket heating, add buffer solution, stir at 200 r / min for 10 min, then add compound enzyme preparation, set the temperature to 40℃, and stir at 150 rpm for 2 h. After the reaction, raise the temperature to 90℃ and hold for 10 min, then perform hot reflux extraction at 90℃ for 1 h. Immediately after extraction, filter through a 200-mesh filter, collect the filtrate, wash the filter residue once with 60℃ hot water, filter again, collect the filtrate, combine the two filtrates, place them in a vacuum concentration tank, and concentrate under reduced pressure at -0.09 MPa and 60℃ to a paste to obtain fir needle extract, thus completing the extraction and processing of fir needles.

[0026] The compound enzyme preparation was prepared by mixing cellulase and pectinase at a mass ratio of 2:1. The buffer solution was prepared by mixing pH 5.0 citrate-disodium hydrogen phosphate solution and L-proline at a mass ratio of 500:1. The mass ratio of buffer solution, compound enzyme preparation and fir powder was 500:15:100. The mass ratio of filter residue to hot water was 1:0.8.

[0027] The extraction method for male paper mulberry flowers is as follows: Select male paper mulberry flower powder and pass it through a 30-mesh sieve. Add it to an ultrasonic extraction tank, then add the extraction solution. Turn on the ultrasonic waves, set the power to 300W, and extract at 50℃ for 30 minutes. Then filter through a 200-mesh filter, collect the filtrate, wash the residue once with 50% ethanol, filter again, collect the filtrate, combine the two filtrates, place them in a vacuum concentration tank, and concentrate under reduced pressure at -0.08MPa vacuum and 65℃ until a paste is obtained, thus completing the extraction process of male paper mulberry flowers.

[0028] The extraction solution was prepared by mixing trehalose, anhydrous ethanol and deionized water in a mass ratio of 1:120:150. The mass ratio of the extraction solution to the male flower powder of Broussonetia papyrifera was 10:600, and the mass ratio of 50% ethanol to the filter residue was 1:0.8.

[0029] The extraction and processing method of Suaeda salsa seeds is as follows: Select Suaeda salsa seed powder and pass it through a 50-60 mesh sieve. Add the Suaeda salsa seed powder and reaction solution to the reaction vessel at a ratio of 5:400. Stir at 100 r / min for 1 h at 60℃. Then, slowly adjust the pH of the system to 6.5 by adding 1.0 mol / L hydrochloric acid solution dropwise. After adjustment, place it in a centrifuge and centrifuge at 5000 r / min for 15 min. Collect the supernatant and place it in a vacuum concentration tank. Concentrate it under reduced pressure at -0.09 MPa vacuum and 70℃ until it becomes a paste to obtain Suaeda salsa seed extract, thus completing the extraction and processing of Suaeda salsa seeds.

[0030] The reaction solution was prepared by dissolving sodium hydroxide and potassium silicate in deionized water at a mass ratio of 8:15, with the mass ratio of deionized water to sodium hydroxide being 240:1.

[0031] The compound extract was obtained by adding fir needle leaf extract, mulberry male flower extract and Suaeda salsa seed extract to a reaction vessel and stirring at 50°C for 20 minutes.

[0032] The wetting agent is alkylphenol polyoxyethylene ether, the antifreeze agent is ethylene glycol, and the preservative is potassium sorbate.

[0033] A method for preparing a highly effective plant growth regulator containing mepiquat chloride includes the following steps: Step 1: Add half the weight percentage of water, antifreeze, preservative and wetting agent to the preparation vessel, and stir at 200 r / min for 5 min to form a uniform mixed mother liquor; Step 2: While stirring, add potassium indolebutyrate and mepiquat chloride (by mass percentage) to the mixed mother liquor from Step 1, increase the speed to 400 r / min, control the material temperature at 40℃, and stir for 20 min to obtain a transparent solution. Step 3: Add the mass percentage of the compound extract to the transparent solution obtained in Step 2, and continue stirring for 20 minutes at 40℃ and 400r / min. Stop heating, add the remaining water, adjust the speed to 300r / min, and stir for 10 minutes to obtain the coarse material. Step 4: Filter the coarse material through a 200-mesh filter, collect the filtrate, and complete the preparation of the high-efficiency plant growth regulator.

[0034] Example 2:

[0035] A highly effective plant growth regulator containing mepiquat chloride comprises the following raw materials in weight percentages: 18% mepiquat chloride, 1.1% potassium indolebutyrate, 5% compound extract, 5% humectant, 5% antifreeze, and 0.3% preservative, with the balance being water.

[0036] The preparation of the compound extract includes the following steps: extracting fir needles, paper mulberry male flowers and Suaeda salsa seeds to obtain corresponding extracts, and mixing the extracts in a 1:1:1 ratio to complete the preparation of the compound extract.

[0037] The extraction and processing method of fir needles is as follows: Select dried fir needles, pulverize them, and pass them through a 40-mesh sieve to obtain fir powder. Place the fir powder in a multi-functional hot reflux extraction tank equipped with stirring and jacket heating, add buffer solution, stir at 250 r / min for 15 min, then add compound enzyme preparation, set the temperature to 45℃, and stir at 150 rpm for 2 h. After the reaction, raise the temperature to 90℃ and hold for 11 min, then perform hot reflux extraction at 95℃ for 1 h. Immediately after extraction, filter through a 250-mesh filter, collect the filtrate, wash the filter residue once with 60℃ hot water, filter again, collect the filtrate, combine the two filtrates, place them in a vacuum concentration tank, and concentrate under reduced pressure at -0.09 MPa vacuum and 60℃ to a paste to obtain fir needle extract, thus completing the extraction and processing of fir needles.

[0038] The compound enzyme preparation was prepared by mixing cellulase and pectinase at a mass ratio of 2:1. The buffer solution was prepared by mixing pH 5.0 citrate-disodium hydrogen phosphate solution and L-proline at a mass ratio of 500:1. The mass ratio of buffer solution, compound enzyme preparation and fir powder was 550:17:100. The mass ratio of filter residue to hot water was 1:1.

[0039] The extraction method for male paper mulberry flowers is as follows: Select paper mulberry male flower powder and pass it through a 30-mesh sieve. Add it to an ultrasonic extraction tank, then add the extraction solution. Turn on the ultrasonic waves and set the power to 300W. Extract at 50℃ for 35 minutes. Then filter through a 250-mesh sieve and collect the filtrate. Wash the filter residue once with 50% ethanol, filter again, and collect the filtrate. Combine the filtrates from the two filtrations and place them in a vacuum concentration tank. Concentrate under reduced pressure at -0.08MPa vacuum and 65℃ until it becomes a paste to obtain the paper mulberry male flower extract, thus completing the extraction process of paper mulberry male flowers.

[0040] The extraction solution was prepared by mixing trehalose, anhydrous ethanol and deionized water in a mass ratio of 1:120:150. The mass ratio of the extraction solution to the male flower powder of Broussonetia papyrifera was 15:600, and the mass ratio of 50% ethanol to the filter residue was 1:1.

[0041] The extraction and processing method of Suaeda salsa seeds is as follows: Select Suaeda salsa seed powder and pass it through a 55-mesh sieve. Add the Suaeda salsa seed powder and reaction solution to the reaction vessel at a ratio of 7:400. Stir at 150 r / min for 1.1 h at 65℃. Then, slowly adjust the pH of the system to 6.5 by adding 1.0 mol / L hydrochloric acid solution dropwise. After adjustment, place it in a centrifuge and centrifuge at 5000 r / min for 15 min. Collect the supernatant and place it in a vacuum concentration tank. Concentrate under reduced pressure at -0.09 MPa and 70℃ until it becomes a paste to obtain Suaeda salsa seed extract, thus completing the extraction and processing of Suaeda salsa seeds.

[0042] The reaction solution was prepared by dissolving sodium hydroxide and potassium silicate in deionized water at a mass ratio of 8:17, with the mass ratio of deionized water to sodium hydroxide being 250:1.

[0043] The compound extract was obtained by adding fir needle leaf extract, mulberry male flower extract and Suaeda salsa seed extract to a reaction vessel and stirring at 55°C for 30 min.

[0044] The wetting agent is alkyl polysaccharide glycoside, the antifreeze agent is urea, and the preservative is Kathon.

[0045] A method for preparing a highly effective plant growth regulator containing mepiquat chloride includes the following steps: Step 1: Add half the weight percentage of water, antifreeze, preservative and wetting agent to the preparation vessel, and stir at 300 r / min for 10 min to form a uniform mixed mother liquor; Step 2: While stirring, add potassium indolebutyrate and mepiquat chloride (by mass percentage) to the mixed mother liquor from Step 1, increase the speed to 500 r / min, control the material temperature at 45℃, and stir for 30 min to obtain a transparent solution. Step 3: Add the mass percentage of the compound extract to the transparent solution obtained in Step 2, and continue stirring for 30 minutes at 45℃ and 500 r / min. Stop heating, add the remaining water, adjust the speed to 400 r / min, and stir for 15 minutes to obtain the coarse material. Step 4: Filter the coarse material through a 250-mesh filter, collect the filtrate, and complete the preparation of the high-efficiency plant growth regulator.

[0046] Example 3:

[0047] A highly effective plant growth regulator containing mepiquat chloride comprises the following raw materials in weight percentages: 40% mepiquat chloride, 2% potassium indolebutyrate, 10% compound extract, 8% humectant, 8% antifreeze, and 0.5% preservative, with the balance being water.

[0048] The preparation of the compound extract includes the following steps: extracting fir needles, paper mulberry male flowers and Suaeda salsa seeds to obtain corresponding extracts, and mixing the extracts in a 1:1:1 ratio to complete the preparation of the compound extract.

[0049] The extraction and processing method of fir needles is as follows: Select dried fir needles, pulverize them, and pass them through a 40-mesh sieve to obtain fir powder. Place the fir powder in a multi-functional hot reflux extraction tank equipped with stirring and jacket heating, add buffer solution, stir at 300 r / min for 20 min, then add compound enzyme preparation, set the temperature to 50℃, and stir at 150 rpm for 2 h. After the reaction, raise the temperature to 90℃ and hold for 12 min, then perform hot reflux extraction at 95℃ for 1 h. Immediately after extraction, filter through a 300-mesh filter, collect the filtrate, wash the filter residue once with 60℃ hot water, filter again, collect the filtrate, combine the two filtrates, place them in a vacuum concentration tank, and concentrate under reduced pressure at -0.09 MPa and 60℃ to a paste to obtain fir needle extract, thus completing the extraction and processing of fir needles.

[0050] The compound enzyme preparation was prepared by mixing cellulase and pectinase at a mass ratio of 2:1. The buffer solution was prepared by mixing pH 5.0 citrate-disodium hydrogen phosphate solution and L-proline at a mass ratio of 500:1. The mass ratio of buffer solution, compound enzyme preparation and fir powder was 600:20:100. The mass ratio of filter residue to hot water was 1:1.2.

[0051] The extraction method for male paper mulberry flowers is as follows: Select male paper mulberry flower powder and pass it through a 30-mesh sieve. Add it to an ultrasonic extraction tank, then add the extraction solution. Turn on the ultrasonic waves, set the power to 300W, and extract at 50℃ for 40 minutes. Then filter through a 300-mesh filter, collect the filtrate, wash the residue once with 50% ethanol, filter again, and collect the filtrate. Combine the two filtrates and place them in a vacuum concentration tank. Concentrate under reduced pressure at -0.08MPa and 65℃ until a paste is obtained, thus completing the extraction process of male paper mulberry flowers.

[0052] The extraction solution was prepared by mixing trehalose, anhydrous ethanol and deionized water in a mass ratio of 1:120:150. The mass ratio of the extraction solution to the male flower powder of Broussonetia papyrifera was 20:600, and the mass ratio of 50% ethanol to the filter residue was 1:1.2.

[0053] The extraction and processing method of Suaeda salsa seeds is as follows: Suaeda salsa seed powder is passed through a 60-mesh sieve. The Suaeda salsa seed powder and reaction solution are added to a reaction vessel at a ratio of 10:400. The mixture is stirred at 200 r / min for 1.2 h at 70 °C. Then, 1.0 mol / L hydrochloric acid solution is added dropwise to slowly adjust the pH of the system to 7.0. After adjustment, the mixture is placed in a centrifuge and centrifuged at 6000 r / min for 20 min. The supernatant is collected and placed in a vacuum concentration tank. The supernatant is concentrated under reduced pressure at -0.09 MPa and 70 °C until it becomes a paste, thus obtaining the Suaeda salsa seed extract and completing the extraction and processing of Suaeda salsa seeds.

[0054] The reaction solution was prepared by dissolving sodium hydroxide and potassium silicate in deionized water at a mass ratio of 8:20, with the mass ratio of deionized water to sodium hydroxide being 260:1.

[0055] The compound extract was obtained by adding fir needle leaf extract, mulberry male flower extract and Suaeda salsa seed extract to a reaction vessel and stirring at 60°C for 40 min.

[0056] The wetting agent is sorbitan oleate, the antifreeze agent is glycerol, and the preservative is potassium sorbate.

[0057] A method for preparing a highly effective plant growth regulator containing mepiquat chloride includes the following steps: Step 1: Add half the weight percentage of water, antifreeze, preservative and wetting agent to the preparation vessel, and stir at 400 r / min for 15 min to form a uniform mixed mother liquor; Step 2: While stirring, add potassium indolebutyrate and mepiquat chloride (by mass percentage) to the mixed mother liquor from Step 1, increase the speed to 600 r / min, control the material temperature at 45℃, and stir for 40 min to obtain a transparent solution. Step 3: Add the mass percentage of the compound extract to the transparent solution obtained in Step 2, and continue stirring for 40 minutes at 50℃ and 600 r / min. Stop heating, add the remaining water, adjust the speed to 500 r / min, and stir for 20 minutes to obtain the coarse material. Step 4: Filter the coarse material through a 300-mesh filter, collect the filtrate, and complete the preparation of the high-efficiency plant growth regulator.

[0058] Comparative Example 1: The difference between this comparative example and Example 1 is that this comparative example does not contain the compound extract.

[0059] Comparative Example 2 differs from Example 1 in that the composite extract in this comparative example does not contain fir needle extract.

[0060] Comparative Example 3 differs from Example 1 in that the composite extract of this comparative example does not contain extract of male flowers of Broussonetia papyrifera.

[0061] Comparative Example 4 differs from Example 1 in that the composite extract in this comparative example does not contain Suaeda salsa seed extract.

[0062] Performance testing: Biochemical tests were conducted on the growth regulators treated in Examples 1-3 and Comparative Examples 1-4. Biochemical test 1: The application was carried out by spraying 40g of the pesticide per acre diluted in 30kg of water. The selected wheat variety was Jimai 22. For wheat agronomic trait testing, transplanted seedlings with uniform growth from each treatment were selected. Following the wheat agronomic trait survey and measurement methods, plant height, stem diameter, root fresh weight, and above-ground fresh weight were measured 15 days after application. Chlorophyll content and ash content were measured 30 days later. The test data are recorded in Table 1. Table 1:

[0063] As shown in Table 1: Using the complete compound extract (fir needles + mulberry male flowers + Suaeda salsa seeds), plant height was well controlled, and stem diameter, root and above-ground fresh weight, chlorophyll content, and ash content were significantly better than the control group. This indicates that the compound extract, in synergy with mepiquat chloride, effectively regulates plant growth and enhances photosynthetic capacity and mineral accumulation. Control group 1: Without the compound extract, all indicators were the worst, indicating that the compound extract is a key component for improving plant growth quality. Control group 2 lacked the terpenoids and phenolic active substances abundant in fir needles. The lack of endogenous hormone precursors and nutrients provided by the male flowers of the paper mulberry tree in Comparative Example 3 interfered with the plant's own nutrient allocation signals, resulting in a weakened driving force for the transport and accumulation of photosynthetic products to the above-ground parts. In Comparative Example 4, the lack of osmotic regulators and root-promoting factors unique to Suaeda salsa seeds directly led to poor root development, which in turn weakened the absorption capacity of water and nutrients, affecting the overall stability and growth of the plant.

[0064] Live test experiment 2: A live test safety experiment was conducted on wheat seed treatment using a dosage of 2.5g / 30 catties of seeds diluted with 30g of water. The treatment was carried out in indoor germination boxes and indoor potted plants in nutrient soil. Jimai 22 wheat was used, with an untreated control group. Germination rate was measured daily in the germination boxes the day after application, and plant height was measured on day 7 post-treatment. For potted plants, plant height, fresh weight per 100 leaves, and dry weight were measured 15 days after sowing. The test data are recorded in Table 2. Table 2:

[0065] As shown in Table 2: Examples 1-3: The addition of the compound extract significantly improved seed germination potential, germination rate, and emergence rate, resulting in higher seedling leaf fresh weight and dry weight, and better plant height control, indicating that the compound extract has a synergistic effect in promoting seed vigor and seedling growth; Comparative Example 1: Without the compound extract, seed vigor and seedling growth indicators were the worst; In Comparative Example 2, the lack of active substances in fir needles led to insufficient energy metabolism in the early stage of seed germination, resulting in slow germination initiation (low germination potential); at the same time, the photosynthetic capacity and material accumulation efficiency of seedling leaves decreased, resulting in low dry weight; In Comparative Example 3, the lack of nutrients and regulatory substances in male flowers of paper mulberry affected seedling cell division and expansion, resulting in insufficient leaf biomass accumulation; at the same time, its deficiency weakened the ability to transport photosynthetic products to leaves; In Comparative Example 4, the lack of root-promoting and stress-resistant components in Suaeda salsa seeds led to poor seedling root development, affecting water absorption and seedling uniformity; at the same time, the plant's water retention capacity was weak, directly manifested in its lowest leaf fresh weight among all comparative examples. Experiment 3: The product was applied evenly as a spray at a dosage of 40g per mu (approximately 0.067 hectares) diluted in 30kg of water. The wheat variety used was Yannong 1212, and the sowing rate was 30 jin (approximately 15 kg) per mu. Winter wheat fields with flat terrain, uniform water and fertilizer distribution, and at the three-leaf-one-heart stage were selected. Equal amounts of the product were applied using drone aerial spraying. Fifteen days after application, the average number of tillers was measured at randomly selected sites. Five days before harvest the following year, the average number of ears per mu, average number of grains per ear, and thousand-grain weight were measured according to the theoretical yield survey method for wheat fields. The theoretical yield and yield increase rate were calculated. The test data are recorded in Table 3. Table 3:

[0066] According to Table 3, in Examples 1-3, the compound extract significantly increased the number of tillers, panicles, grains per panicle, and thousand-grain weight. The final yield and yield increase rate increased with the increase of dosage, indicating that it has a synergistic effect of comprehensively optimizing the yield composition. In Comparative Example 1, there was no compound extract, and the yield composition indicators and yield increase rate were the lowest. In Comparative Example 2, the lack of active ingredients from fir needles reduced the photosynthetic efficiency and stress resistance of the plants, resulting in insufficient energy accumulation, which limited the growth of tiller and grains per panicle and made the yield increase weak. In Comparative Example 3, the lack of extract from male flowers of paper mulberry affected key signals of reproductive development, hindered the differentiation of florets in the panicle and grain formation, and reduced the efficiency of photosynthetic product allocation to the panicle. In Comparative Example 4, the lack of root-promoting substances unique to Suaeda salsa seeds resulted in poor root development, weakened nutrient absorption and stress resistance, and directly affected tillering, panicle formation, and grain filling.

[0067] By comparing and analyzing the relevant data in Tables 1, 2, and 3, it can be seen that the plant growth regulator prepared in this invention not only has significant effects in controlling excessive growth, resisting stress, and promoting root and seedling growth, but also has excellent synergistic effects and yield-increasing and quality-improving properties, effectively enhancing the overall growth quality and final yield of crops. This indicates that the high-efficiency plant growth regulator containing mepiquat chloride provided by this invention has a broader market prospect and is more suitable for promotion.

[0068] In the description of this specification, references to terms such as "an embodiment," "example," and "specific example" indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the invention. In this specification, illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0069] The preferred embodiments of the present invention disclosed above are merely illustrative of the invention. These preferred embodiments do not exhaustively describe all details, nor do they limit the invention to the specific implementations described. Clearly, many modifications and variations can be made based on the content of this specification. This specification selects and specifically describes these embodiments to better explain the principles and practical applications of the invention, thereby enabling those skilled in the art to better understand and utilize the invention. The invention is limited only by the claims and their full scope and equivalents.

Claims

1. A highly efficient plant growth regulator containing mepiquat chloride, characterized in that, The raw material composition includes the following percentages by weight: mepiquat chloride 5-40%, potassium indolebutyrate 0.2-2%, compound extract 1-10%, humectant 2-8%, antifreeze 2-8%, and preservative 0.1-0.5%, with the balance being water; The preparation of the compound extract includes the following steps: The leaves of fir needles, the male flowers of paper mulberry and the seeds of Suaeda salsa were extracted to obtain the corresponding extracts of fir needles, paper mulberry male flowers and Suaeda salsa seeds. Fir needle leaf extract, paper mulberry male flower extract and Suaeda salsa seed extract were added to a reaction vessel in a mass ratio of 1:1:1 and stirred at 50-60℃ for 20-40 minutes until homogeneous to obtain the composite extract. The extraction and processing method of fir needles is as follows: dried fir needles are selected, pulverized, and passed through a 40-mesh sieve to obtain fir powder. The fir powder is placed in a multi-functional hot reflux extraction tank with stirring and jacket heating, a buffer solution is added, and the mixture is stirred at 200-300 r / min for 10-20 min. Then, a compound enzyme preparation is added, the temperature is set at 40-50℃, and the mixture is stirred at 150 rpm for 2 h. After the reaction, the temperature is raised to 90℃ and held for 10-12 min. Then, the mixture is hot refluxed at 90-95℃ for 1 h. The mixture is then immediately removed and filtered through a 200-300 mesh filter. The filtrate is collected, and the residue is washed once with 60℃ hot water. The mixture is filtered again and the filtrate is collected. The filtrates from the two filtrations are combined and placed in a vacuum concentration tank. The mixture is concentrated under reduced pressure at -0.09 MPa and 60℃ to a paste to obtain fir needle extract. The compound enzyme preparation is prepared by mixing cellulase and pectinase at a mass ratio of 2:

1. The buffer solution is prepared by mixing pH 5.0 citrate-disodium hydrogen phosphate solution and L-proline at a mass ratio of 500:

1. The mass ratio of the buffer solution, compound enzyme preparation and fir powder is 500-600:15-20:

100. The mass ratio of the filter residue to hot water is 1:(0.8-1.2). The extraction and processing method of the male flowers of Broussonetia papyrifera is as follows: Select the powder of male flowers of Broussonetia papyrifera and pass it through a 30-mesh sieve. Add it to an ultrasonic extraction tank, then add the extraction solution, turn on the ultrasonic waves, set the power to 300W, and extract at 50℃ for 30-40 minutes. Then filter it through a 200-300 mesh sieve, collect the filtrate, wash the filter residue once with 50% ethanol, filter again and collect the filtrate. Combine the filtrates from the two filtrations and place them in a vacuum concentration tank. Concentrate under reduced pressure at -0.08MPa vacuum and 65℃ until it becomes a paste to obtain the extract of male flowers of Broussonetia papyrifera. The extraction solution is prepared by mixing trehalose, anhydrous ethanol and deionized water in a mass ratio of 1:120:

150. The mass ratio of the extraction solution to the male flower powder of Broussonetia papyrifera is 10-20:

600. The mass ratio of 50% ethanol to the filter residue is 1:(0.8-1.2). The extraction and processing method of Suaeda salsa seeds is as follows: Select Suaeda salsa seed powder and pass it through a 50-60 mesh sieve. Add the Suaeda salsa seed powder and reaction solution to a reaction vessel at a mass ratio of 5-10:

400. Stir at 100-200 r / min for 1-1.2 h at 60-70℃. Then, slowly adjust the pH of the system to 6.5-7.0 by adding 1.0 mol / L hydrochloric acid solution dropwise. After adjustment, place it in a centrifuge and centrifuge at 5000-6000 r / min for 15-20 min. Collect the supernatant and place it in a vacuum concentration tank. Concentrate it under reduced pressure at -0.09 MPa vacuum and 70℃ until it becomes a paste to obtain Suaeda salsa seed extract. The reaction solution is prepared by dissolving sodium hydroxide and potassium silicate in deionized water at a mass ratio of 8:15-20, wherein the mass ratio of deionized water to sodium hydroxide is 240-260:

1. The plant growth regulator is prepared by the following method: Step 1: Add half the amount of water, antifreeze, preservative and wetting agent to the preparation vessel, and stir at 200-400 r / min for 5-15 min to form a uniform mixed mother liquor. Step 2: While stirring, add the potassium indolebutyrate and mepiquat chloride to the mixed mother liquor obtained in Step 1, increase the rotation speed to 400-600 r / min, control the material temperature at 40-50℃, and stir for 20-40 min to obtain a transparent solution. Step 3: Add the composite extract to the transparent solution obtained in Step 2, and continue stirring for 20-40 minutes at 40-50℃ and 400-600 r / min. Stop heating, add the remaining water, adjust the speed to 300-500 r / min, and stir for 10-20 minutes to obtain the coarse material. Step 4: Filter the coarse material through a 200-300 mesh filter and collect the filtrate to obtain the highly efficient plant growth regulator. The wetting agent is one or more of alkylphenol polyoxyethylene ether, alkyl polysaccharide glycoside, betaine, sorbitan oleate, polyoxyethylene 20 sorbitan monooleate, and sodium fatty alcohol polyoxyethylene ether sulfate; the antifreeze agent is one or more of ethylene glycol, glycerol, and urea; and the preservative is one or more of potassium sorbate and Kathon.

2. The highly efficient plant growth regulator containing mepiquat chloride according to claim 1, characterized in that, The wetting agent is an alkyl polysaccharide glycoside, the antifreeze agent is urea, and the preservative is Kathon.

3. The highly efficient plant growth regulator containing mepiquat chloride according to claim 1 or 2, characterized in that, The mass percentages of the mepiquat chloride are 18%, potassium indolebutyrate is 1.1%, compound extract is 5%, humectant is 5%, antifreeze is 5%, and preservative is 0.3%.

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