The invention discloses mRNA (messenger ribonucleic acid) 5apos capable of enhancing mRNA translation efficiency. UTR sequence and application thereof

Abstract

The invention discloses mRNA 5 'UTR (Untranslated Region) sequences for enhancing mRNA translation efficiency and application of the mRNA 5' UTR sequences, and belongs to the technical field of biology. According to the mRNA 5 'UTR sequence for enhancing the mRNA translation efficiency, provided by the invention, the protein expression quantities of mRNA for coding firefly luciferase (Fluc) in a Hela cell line, an HEK293T cell line and a mouse body are respectively 3.4 times, 1.6 times and 2 times higher than those of a 5' UTR sequence (contrast) used in a Luy / BioNTeck COVID-19 vaccine BNT162b2, and the expression duration is longer; the protein expression quantities of mRNA for coding enhanced green fluorescent protein (eGFP) in two cell lines of Hela and HEK293T are higher than that of a control; the expression quantity of a neutralizing antibody induced in a mouse model by using the COVID-19mRNA candidate vaccine with the 5 'UTR sequence provided by the invention is 2.5 times higher than that of a control group; the 5 'UTR sequence provided by the invention is suitable for various mRNAs, and can be applied to preparation of vaccines, research and development of local injection materials and application in protein expression tools.

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