Composite disinfectant and use thereof

Abstract

The application discloses a composite disinfectant and application thereof. The disinfectant independently researched and developed by the application has strong bactericidal effect, is environment-friendly, green and environment-friendly, high in safety, low in molecular weight, good in water solubility, low in cost, and wide in application prospect.

Description

Technical Field

[0001] This invention belongs to the field of biomedicine, specifically relating to a compound disinfectant and its application. Background Technology

[0002] Disinfectants are used to kill pathogenic microorganisms on a medium, rendering it harmless. In recent years, they have received increasing attention in public places and personal care. Disinfection and sterilization have become almost essential in daily work and life. Generally, they can be categorized into several applications, including air disinfection, skin disinfection, surface disinfection of objects, and disinfection of medical devices.

[0003] In the field of medical devices, especially dental devices, the actual implementation of disinfection for dental unit waterlines (DUWLs) is not ideal, mainly due to the lack of suitable disinfection products. In recent years, although more than ten local and group standards have been issued, setting forth specific requirements for DUWL contamination control at the regulatory level, reflecting the industry's high level of attention and concern about this issue, the lack of efficient, economical, and convenient local disinfection solutions, coupled with the lack of breakthroughs in related technologies and product development, makes it difficult to fully implement these standards in practice, severely limiting their clinical application. Therefore, the development of compound disinfectants suitable for my country's national conditions and capable of effectively controlling DUWL contamination is urgently needed. This will not only fill the product and technology gaps in related domestic fields but also provide a solid guarantee for improving the level of biosafety management of dental treatment waterlines in my country. Summary of the Invention

[0004] To overcome the shortcomings of existing technologies, this invention provides a composite disinfectant and its application.

[0005] To achieve the above objectives, the present invention adopts the following technical solution:

[0006] A first aspect of the present invention provides a composite disinfectant, wherein the disinfectant components include α-hydroxy acid, chelating agent, peroxide, metal corrosion inhibitor, chitosan, and AgNO3.

[0007] Furthermore, the α-hydroxy acid is selected from citric acid.

[0008] Furthermore, the chelating agent is selected from EDTA.

[0009] Furthermore, the peroxide is selected from sodium percarbonate.

[0010] Furthermore, the metal corrosion inhibitor is selected from sodium benzoate.

[0011] Furthermore, the chitosan is selected from biguanide chitosan.

[0012] Furthermore, the proportion of citric acid is 60%-70%.

[0013] Furthermore, the proportion of citric acid is 64.76%.

[0014] Furthermore, the proportion of EDTA is 12%-20%.

[0015] Furthermore, the proportion of EDTA is 14.39%.

[0016] Furthermore, the proportion of sodium percarbonate is 12%-20%.

[0017] Furthermore, the proportion of sodium percarbonate is 14.39%.

[0018] Furthermore, the proportion of sodium benzoate is 3%-10%.

[0019] Furthermore, the proportion of sodium benzoate is 4.30%.

[0020] Furthermore, the proportion of biguanide chitosan is 1%-5%.

[0021] Furthermore, the proportion of biguanide chitosan is 2.00%.

[0022] Furthermore, the proportion of AgNO3 is <1%.

[0023] Furthermore, the proportion of AgNO3 is 0.16%.

[0024] Furthermore, the disinfectant also includes one or more additional ingredients.

[0025] Furthermore, the additional ingredients include pH adjusters, buffers, nonionic surfactants, water-soluble growth promoters, rheology modifiers, preservatives, or any combination thereof.

[0026] Furthermore, the disinfectant includes both solid and liquid forms.

[0027] Furthermore, the solid form of the disinfectant includes tablets and powders.

[0028] A second aspect of the present invention provides a disinfection and sterilization product, the product comprising the disinfectant described in the first aspect of the present invention.

[0029] The third aspect of the present invention provides the application of the disinfectant described in the first aspect of the present invention or the product described in the second aspect of the present invention in disinfection and sterilization.

[0030] Furthermore, the bacteria include Gram-positive bacteria and Gram-negative bacteria.

[0031] Furthermore, the Gram-positive bacteria are selected from Gram-positive cocci.

[0032] Furthermore, the Gram-positive cocci are selected from Staphylococcus aureus.

[0033] Furthermore, the Gram-negative bacteria are selected from Gram-negative bacilli.

[0034] Furthermore, the Gram-negative bacilli are selected from Pseudomonas aeruginosa.

[0035] Furthermore, the disinfection and sterilization include waterway disinfection and sterilization, surface disinfection and sterilization, and environmental disinfection and sterilization.

[0036] Furthermore, the water system disinfection and sterilization refers to the disinfection and sterilization of the medical water system.

[0037] Furthermore, the disinfection and sterilization of the treatment water system refers to the disinfection and sterilization of the water system of the dental treatment unit.

[0038] Furthermore, the surface disinfection and sterilization mentioned herein refers to the surface disinfection and sterilization of oral instruments.

[0039] Furthermore, the environmental disinfection and sterilization refers to oral environmental disinfection and sterilization.

[0040] The fourth aspect of the present invention provides the application of the disinfectant described in the first aspect of the present invention in the preparation of disinfection and sterilization products.

[0041] The fifth aspect of the present invention provides a method for disinfection and sterilization, the method comprising using the disinfectant described in the first aspect of the present invention or the product described in the second aspect of the present invention for disinfection and sterilization.

[0042] Furthermore, the bacteria include Gram-positive bacteria and Gram-negative bacteria.

[0043] Furthermore, the Gram-positive bacteria are selected from Gram-positive cocci.

[0044] Furthermore, the Gram-positive cocci are selected from Staphylococcus aureus.

[0045] Furthermore, the Gram-negative bacteria are selected from Gram-negative bacilli.

[0046] Furthermore, the Gram-negative bacilli are selected from Pseudomonas aeruginosa.

[0047] Furthermore, the disinfection and sterilization include waterway disinfection and sterilization, surface disinfection and sterilization, and environmental disinfection and sterilization.

[0048] Furthermore, the water system disinfection and sterilization refers to the disinfection and sterilization of the medical water system.

[0049] Furthermore, the disinfection and sterilization of the treatment water system refers to the disinfection and sterilization of the water system of the dental treatment unit.

[0050] Furthermore, the surface disinfection and sterilization mentioned herein refers to the surface disinfection and sterilization of oral instruments.

[0051] Furthermore, the environmental disinfection and sterilization refers to oral environmental disinfection and sterilization.

[0052] Furthermore, the method described is an in vitro method for non-therapeutic purposes.

[0053] Advantages and beneficial effects of the present invention:

[0054] The disinfectant independently developed in this application has strong bactericidal power, is environmentally friendly, green and environmentally friendly, has high safety, low molecular weight and good water solubility, and is inexpensive, with broad application prospects. Attached Figure Description

[0055] Figure 1 These are the MIC plots of P. aeruginosa for each experimental group. Among them, 1A is the MIC plot of group 1, 1B is the MIC plot of group 2, 1C is the MIC plot of group 3, 1D is the MIC plot of group 4, 1E is the MIC plot of group 5, 1F is the MIC plot of group 6, 1G is the MIC plot of group 7, 1H is the MIC plot of group 8, 1I is the MIC plot of group 9, and 1J is the MIC plot of group 10.

[0056] Figure 2 These are the MIC plots for each experimental group against S. aureus. Among them, 2A is the MIC plot for group 1, 2B is the MIC plot for group 2, 2C is the MIC plot for group 3, 2D is the MIC plot for group 4, 2E is the MIC plot for group 5, 2F is the MIC plot for group 6, 2G is the MIC plot for group 7, 2H is the MIC plot for group 8, 2I is the MIC plot for group 9, and 2J is the MIC plot for group 10. Detailed Implementation

[0057] The following provides definitions for some of the terms used in this specification. Unless otherwise stated, all technical and scientific terms used herein generally have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains.

[0058] This invention provides a composite disinfectant, the components of which include α-hydroxy acid, chelating agent, peroxide, metal corrosion inhibitor, chitosan, and AgNO3.

[0059] In some embodiments, α-hydroxy acids include, but are not limited to, citric acid, lactic acid, formic acid, glycolic acid, malic acid, mandelic acid, and tartaric acid.

[0060] In a specific implementation, the α-hydroxy acid is selected from citric acid.

[0061] In some embodiments, the chelating agent includes, but is not limited to, EDTA, phosphate, NTA, MGDA, GLDA, citrate, carbonate, and bicarbonate.

[0062] In a specific implementation, the chelating agent is selected from EDTA.

[0063] In some embodiments, the peroxides include, but are not limited to, sodium percarbonate, sodium perborate, peroxyurea, superphosphate, and persilicate.

[0064] In a specific implementation, the peroxide is selected from sodium percarbonate.

[0065] In some embodiments, the metal corrosion inhibitor includes, but is not limited to, sodium benzoate, triethanolamine, diethanolamine, and benzotriazole.

[0066] In a specific implementation, the metal corrosion inhibitor is selected from sodium benzoate.

[0067] In some embodiments, chitosan includes, but is not limited to, biguanide chitosan and quaternary ammonium chitosan.

[0068] In a specific implementation, chitosan is selected from biguanide chitosan.

[0069] The disinfectant also includes one or more additional ingredients.

[0070] The additional ingredients include pH adjusters, buffers, nonionic surfactants, water-soluble growth promoters, rheology modifiers, preservatives, or any combination thereof.

[0071] In some embodiments, suitable pH adjusters include substances exhibiting basic or acidic properties. Examples of substances exhibiting basic properties include, but are not limited to, alkali metal hydroxides such as sodium hydroxide and potassium hydroxide; carbonates such as sodium carbonate, sodium bicarbonate, and potassium carbonate; silicates such as sodium silicate and potassium silicate; borates such as sodium borate; organic acid salts such as sodium citrate; amines such as monoethanolamine and diethanolamine; and ammonia. Examples of substances exhibiting acidic properties and used for pH adjustment include, but are not limited to, inorganic acids such as hydrochloric acid and sulfuric acid, or organic acids such as citric acid and acetic acid.

[0072] In some embodiments, suitable buffers include, but are not limited to, phosphoric acid, monosodium phosphate, disodium phosphate, trisodium phosphate, citric acid, and salts such as sodium citrate, benzoic acid, sodium benzoate, or any combination thereof.

[0073] In some embodiments, suitable nonionic surfactants include, but are not limited to, ethylene oxide adducts of C8 to C22, preferably C8 to C16, more preferably C8 to C12 alcohols; ethylene oxide / propylene oxide adducts of ethylene glycol; alkylene glycols; alkyl polyglucosides; or mixtures thereof. Non-limiting examples of suitable types of nonionic surfactants include ethoxylated alkyl polyethylene glycol ethers, polyalkylene glycols (e.g., 100% Breox FCC92), or alcohol alkoxylated EO / PO. Exemplary alcohol ethoxylated compounds include fatty alcohol ethoxylated compounds, such as tridecyl alcohol alkoxylated compounds, ethylene oxide adducts, alkylphenol ethoxylated compounds, or ethoxy / propoxy block surfactants. In some embodiments, the nonionic surfactant is an alcohol ethoxylated compound, an EO / PO block copolymer, an alkyl polyglucoside, or any combination thereof.

[0074] In some embodiments, suitable water-soluble growth promoters may include, but are not limited to, benzene sulfonates, naphthalene sulfonates, alkylbenzene sulfonates, naphthalene sulfonates, alkyl sulfonates, alkyl sulfates, alkyl diphenyl ether disulfonates, or phosphate ester water-soluble growth promoters. Exemplary alkylbenzene sulfonates include, for example, isopropylbenzene sulfonates, xylene sulfonates, toluene sulfonates, cumene sulfonates, or any mixture of two or more thereof. Exemplary alkyl sulfonates include hexyl sulfonates, octyl sulfonates, and hexyl / octyl sulfonates, or any mixture of two or more thereof.

[0075] In some embodiments, suitable rheology modifiers include, but are not limited to, water-soluble cellulose polymers, such as hydroxyethyl cellulose or hydroxypropyl cellulose.

[0076] In some embodiments, suitable preservatives include, but are not limited to, iodopropynyl butylcarbamate; formaldehyde; benzoic acid esters (para-hydroxybenzoates), such as methylparaben, propylparaben, butylparaben, ethylparaben, isopropylparaben, isobutylparaben, benzylparaben; 2-bromo-2-nitropropane-1,3-diol; benzoic acid; formic acid.

[0077] This invention provides the application of the above-mentioned disinfectant or product in disinfection and sterilization.

[0078] The bacteria include Gram-positive bacteria and Gram-negative bacteria.

[0079] In some embodiments, Gram-positive bacteria include Gram-positive cocci and Gram-positive bacilli. The Gram-positive cocci include Staphylococcus (Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus saprophyticus), Streptococcus (Streptococcus pneumoniae, Group A Streptococcus, Group B Streptococcus, viridans Streptococcus), and Enterococcus (Enterococcus faecalis); the Gram-positive bacilli include aerobic Gram-positive bacilli (Bacillus anthracis, Corynebacterium diphtheriae, Bacillus cereus, Listeria monocytogenes) and anaerobic Gram-positive bacilli (Clostridium tetani, Clostridium perfringens, Clostridium difficile, Clostridium botulinum).

[0080] In some embodiments, Gram-negative bacteria include Gram-negative bacilli and Gram-negative cocci. The Gram-negative bacilli include Enterobacteriaceae (Escherichia coli, Klebsiella pneumoniae, Proteus spp., Salmonella spp., Shigella spp., Enterobacter spp., Citrobacter spp., Serratia spp.), Vibrioceae (Vibrio cholerae, Vibrio parahaemolyticus), Pseudomonas spp. (Pseudomonas aeruginosa), Acinetobacter spp. (Acinetobacter baumannii), Haemophilus spp. (Haemophilus influenzae), Legionella spp. (Legionella pneumophila), Yersinia spp. (Yersinia pestis, Yersinia enterocolitica), and Campylobacter spp. (Campylobacter jejuni); the Gram-negative cocci include Neisseria spp. (Neisseria meningitidis, Neisseria gonorrhoeae) and Moraxella spp. (Moraxella catarrhalis).

[0081] In a preferred embodiment, Gram-positive bacteria are selected from Gram-positive cocci; Gram-negative bacteria are selected from Gram-negative bacilli.

[0082] In a specific implementation, Gram-positive cocci are selected from Staphylococcus aureus; Gram-negative bacilli are selected from Pseudomonas aeruginosa.

[0083] In some embodiments, the disinfectant may be physically and chemically stable during storage. For example, the disinfectant may be in the form of a liquid solution that maintains its physical appearance as a clear solution without detectable phase separation, precipitation, discoloration, or crystallization of the solute during storage. The solution may maintain a constant pH in its components or undergo minimal degradation or chemical changes, including, for example, degradation of its active ingredient of less than 1.0%, less than 0.5%, less than 0.1%, less than 0.05%, or even 0.01%. The storage duration may be, for example, about one month, about two months, about three months, about six months, about nine months, or even about twelve months.

[0084] In some embodiments, the disinfectant may also maintain substantially the same level of chemical and biological activity over the storage period. As used herein, the property of “maintaining” disinfectant activity refers to the ability to retain its disinfectant activity at least 90% of its original activity when the product is freshly prepared. For example, the disinfectant may maintain at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or even at least 99.9% of its disinfectant activity for a period of at least two weeks, at least one month, at least two months, at least three months, or even at least six months. The disinfectant may maintain about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98%, about 99%, about 99.5%, or even about 99.9% of its disinfectant activity for a period of at least one month or at least three months. In some implementations, the disinfectant retains approximately 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 99.5%, or even 99.9% of its disinfecting and bactericidal activity for a period of at least three months.

[0085] In some embodiments, the disinfectant can be stored stably in a temperature range of about 0°C to about 50°C, including, for example, about 0°C to about 45°C, about 0°C to about 40°C, about 0°C to about 35°C, about 0°C to about 30°C, about 0°C to about 25°C (or room temperature), or about 0°C to about 20°C. For example, the disinfectant can maintain its disinfecting and bactericidal activity for a period of at least two weeks, at least one month, at least two months, at least three months, or even at least six months when stored at temperatures of about 0°C, about 5°C, about 10°C, about 15°C, about 20°C, about 25°C, about 30°C, about 35°C, about 40°C, about 45°C, or even about 50°C. In some embodiments, the disinfectant maintains about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98%, about 99%, about 99.5%, or even about 99.9% of its disinfecting and bactericidal activity when stored in a temperature range of about 0°C to about 40°C for at least one month or at least three months. In some embodiments, the disinfectant retains approximately 90%, approximately 91%, approximately 92%, approximately 93%, approximately 94%, approximately 95%, approximately 96%, approximately 97%, approximately 98%, approximately 99%, approximately 99.5%, or even approximately 99.9% of its disinfectant activity when stored at a temperature of approximately 0°C, approximately 25°C, or approximately 40°C for at least a period of at least three months.

[0086] In some embodiments, the disinfectant is in solid form, such as powder or tablets; or in liquid form, such as solution or suspension. In some embodiments, the disinfectant is a powder that can be dissolved by a user with a suitable solvent (such as water) to prepare a liquid disinfectant. In some embodiments, the disinfectant is a ready-to-use solution. In some embodiments, the disinfectant is a liquid concentrate that can be diluted by a user with a suitable diluent at a concentrate / diluent ratio of, for example, 1:1 to 1:1000 by weight. This includes concentrate / diluent ratios of about 1:5, about 1:10, about 1:20, about 1:50, about 1:100, about 1:200, or about 1:500 by weight.

[0087] The invention is further illustrated below with reference to specific embodiments. It should be understood that the specific embodiments described herein are by way of example and are not intended to limit the invention. The main features of the invention can be used in various embodiments without departing from the scope of the invention.

[0088] Example

[0089] 1. Experimental Materials

[0090] The specific experimental materials are shown in Table 1.

[0091] Table 1 List of materials required for the experiment

[0092]

[0093] 2 Experimental Methods

[0094] Using *Staphylococcus aureus* and *Pseudomonas aeruginosa* as model strains, Luria-Bertany (LB) liquid medium was prepared by adding 0.5 g sodium chloride, 1 g tryptone, and 0.5 g yeast extract to 100 mL of deionized water. Tryptone-soybean agar (TSA) solid medium was prepared according to the manufacturer's instructions. Both bacterial strains were added to the LB medium and cultured in a shaker at 37 °C for 12 h. The OD value of the bacterial solution at 500 nm was measured using a micro-spectrophotometer and found to be in the range of 0.6–0.8, indicating that the bacteria had reached the logarithmic growth phase and were ready for further testing. The bacterial solution was diluted with LB medium to an initial concentration of 2 × 10⁻⁶. 5 CFU / mL. EDTA tetrasodium dihydrate, sodium benzoate, and cationic polymer were dissolved in a certain amount of water according to the formula, stirred until homogeneous, and freeze-dried into powder. This powder was then mixed with citric acid, sodium percarbonate, and AgNO3 powder to prepare a mixed disinfectant. A 96-well plate was prepared, and different concentrations and components of the mixed disinfectant (Table 2) were co-cultured with the bacterial strain in the wells using a micro-dilution method. The final disinfectant concentration ranged from 1024 mg / L to 0 mg / L. The plates were incubated for 12 hours at 37 ℃. The absorbance at 600 nm was read using a microplate reader, and the minimum inhibitory concentration (MIC) was calculated.

[0095] 3 Experimental Results

[0096] The MICs for all groups of two strains were calculated and are shown in Table 3. Figure 1 and Figure 2 As shown, since Pseudomonas aeruginosa is the dominant strain in dental waterline systems (DUWLs), experimental group 8 was selected as the final disinfectant formulation after comprehensive consideration.

[0097] Table 2 Experimental Groups

[0098]

[0099] Table 3 Sterilization effect

[0100]

[0101] The above description of the embodiments is only for understanding the method and core ideas of the present invention. It should be noted that those skilled in the art can make various improvements and modifications to the present invention without departing from the principles of the invention, and these improvements and modifications will also fall within the protection scope of the claims of the present invention.

Claims

1. A composite disinfectant, characterized by comprising: The disinfectant components include α-hydroxy acids, chelating agents, peroxides, metal corrosion inhibitors, chitosan, and AgNO3; The α-hydroxy acid is selected from citric acid; The chelating agent is selected from EDTA; The peroxide is selected from sodium percarbonate; The metal corrosion inhibitor is selected from sodium benzoate; The chitosan is selected from biguanide chitosan; The proportion of citric acid is 60%-70%; The proportion of EDTA is 12%-20%; The proportion of sodium percarbonate is 12%-20%; The proportion of sodium benzoate is 3%-10%; The proportion of biguanide chitosan is 1%-5%; The proportion of AgNO3 is <1%.

2. The disinfectant according to claim 1, characterized in that, The disinfectant also includes one or more additional ingredients.

3. The disinfectant of claim 2, wherein, The additional ingredients include pH adjusters, buffers, nonionic surfactants, water-soluble growth promoters, rheology modifiers, preservatives, or any combination thereof.

4. The disinfectant according to claim 1, characterized in that, The disinfectant includes both solid and liquid forms.

5. The disinfectant according to claim 4, characterized in that, The solid form of disinfectant includes tablets and powders.

6. A disinfection and sterilization product, characterized in that, The product includes the disinfectant as described in any one of claims 1-5.

7. The use of the disinfectant according to any one of claims 1-5 or the product according to claim 6 in disinfection and sterilization for non-therapeutic purposes, wherein the bacteria include Gram-positive bacteria and Gram-negative bacteria; The Gram-positive bacteria are selected from Gram-positive cocci; The Gram-positive cocci were selected from Staphylococcus aureus; The Gram-negative bacteria are selected from Gram-negative bacilli; The Gram-negative bacilli were selected from Pseudomonas aeruginosa.

8. The application according to claim 7, characterized in that, The disinfection and sterilization include waterway disinfection and sterilization, surface disinfection and sterilization, and oral environment disinfection and sterilization.

9. The application according to claim 8, characterized in that, The water system disinfection and sterilization refers to the disinfection and sterilization of the diagnostic and treatment water system.

10. The application according to claim 9, characterized in that, The disinfection and sterilization of the treatment water system refers to the disinfection and sterilization of the water system of the dental treatment unit.

11. The application according to claim 8, characterized in that, The surface disinfection and sterilization mentioned refers to the surface disinfection and sterilization of oral instruments.

12. The use of the disinfectant according to any one of claims 1-5 in the preparation of disinfection and sterilization products.

13. A method for disinfection and sterilization for non-therapeutic purposes, characterized in that, The method includes disinfection and sterilization using the disinfectant according to any one of claims 1-5 or the product according to claim 6.

14. The method according to claim 13, characterized in that, The bacteria include Gram-positive bacteria and Gram-negative bacteria; The Gram-positive bacteria are selected from Gram-positive cocci; The Gram-positive cocci were selected from Staphylococcus aureus; The Gram-negative bacteria are selected from Gram-negative bacilli; The Gram-negative bacilli were selected from Pseudomonas aeruginosa.

15. The method according to claim 13, characterized in that, The disinfection and sterilization include waterway disinfection and sterilization, surface disinfection and sterilization, and oral environment disinfection and sterilization.

16. The method according to claim 15, characterized in that, The water system disinfection and sterilization refers to the disinfection and sterilization of the diagnostic and treatment water system.

17. The method according to claim 16, characterized in that, The disinfection and sterilization of the treatment water system refers to the disinfection and sterilization of the water system of the dental treatment unit.

18. The method according to claim 15, characterized in that, The surface disinfection and sterilization mentioned refers to the surface disinfection and sterilization of oral instruments.

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