Reproduction apparatus and reproduction method for sea anemone

Abstract

The present application relates to the technical field of marine product cultivation, in particular to a sea anemone breeding device and method, which comprises a support, a temporary breeding cylinder, a circulation pipeline, a flow guide pipeline, a cutting breeding cylinder and a circulation pump. The support is fixed on the ground, and the temporary breeding cylinder and the cutting breeding cylinder are fixed on the support respectively. The flow guide outlet on the side of the temporary breeding cylinder is connected with the flow guide inlet above the cutting breeding cylinder through the flow guide pipeline. The circulation outlet at the bottom of the cutting breeding cylinder is connected with the circulation inlet above the temporary breeding cylinder through the circulation pipeline. The circulation pump is installed on the circulation pipeline. Control valves are installed on the circulation pipeline and the flow guide pipeline respectively. The cutting device for cutting the sea anemone evenly is installed in the cutting breeding cylinder. The sea anemone is bred through the steps of temporary breeding, cutting and growth repair, and the device has the advantages of simple structure, high breeding efficiency and good breeding effect.

Description

Technical Field

[0001] This invention relates to the field of marine aquaculture technology, specifically to a simple, efficient, and effective breeding device and method for sea anemones. Background Technology

[0002] As is well known, sea anemones, as an important component of marine biological resources, are increasingly demonstrating their economic and ecological value. In recent years, the market price of sea anemones has continued to rise, surpassing many traditional seafoods, reflecting a significant increase in market demand. This trend is mainly driven by three factors: the catering industry has innovatively developed sea anemone-themed dishes, making them a new culinary favorite; the biopharmaceutical field has discovered the potential of sea anemone toxins in the development of analgesic and anticancer drugs, increasing their added value; and the ornamental aquarium market has seen a steady increase in demand for sea anemones. my country's sea anemone resources are distributed in a pattern of "highest concentration in the South China Sea, followed by the Yellow and Bohai Seas, and lowest concentration in the East China Sea," and this regional difference provides space for specialized development. As sea anemones have transformed from a "netting nuisance" for fishermen to a "marine delicacy," their industrial development is ushering in new opportunities, but also faces challenges such as sustainable resource utilization and quality standardization. The indicative role of sea anemones in the marine ecosystem and their potential in multiple application areas make them a promising emerging species worthy of close attention in the marine economy.

[0003] Asexual reproduction, a key technology in sea anemone farming, primarily achieves efficient proliferation through artificially triggered division mechanisms. Specific methods include: selecting healthy adults for longitudinal or transverse cutting, utilizing the sea anemone's strong regenerative ability to allow fragments to develop into new individuals; or inducing spontaneous division through environmental stimuli. The core advantage of this technology lies in maintaining consistent superior traits in the parent organism, significantly shortening the reproductive cycle, and reducing seedling costs. However, this technology currently suffers from significant drawbacks: firstly, insufficient standardization, with cutting mainly done manually, and a lack of scientific specifications for cutting location and size, leading to large fluctuations in survival rates (typically between 40% and 70%); secondly, deformities or developmental delays are prone to occur during regeneration, affecting the uniformity of commercial specifications; and thirdly, long-term asexual reproduction leads to a decline in population genetic diversity, increasing the risk of disease outbreaks. Furthermore, before artificial cutting, the sea anemones shrink into a ball due to operational stimuli, drastically reducing their volume and increasing the difficulty of precise cutting. These bottlenecks severely restrict the large-scale development of sea anemone farming and urgently require breakthroughs through patented technologies, such as developing specialized cutting devices and standardized operating procedures, to achieve industrial upgrading. Summary of the Invention

[0004] The purpose of this invention is to overcome the shortcomings of the prior art and provide a breeding device and method for sea anemones that is simple in structure, has high breeding efficiency, and good breeding effect.

[0005] The technical solution adopted by this invention to solve its technical problem is: A breeding device for sea anemones, characterized in that the device includes a support frame, a temporary holding tank, a circulation pipeline, a guide pipeline, a cutting breeding tank, and a circulation pump. The support frame is fixed to the ground, and the temporary holding tank and the cutting breeding tank are respectively fixed on the support frame. The guide outlet on the side of the temporary holding tank is connected to the guide inlet above the cutting breeding tank via the guide pipeline. The circulation outlet at the bottom of the cutting breeding tank is connected to the circulation inlet above the temporary holding tank via the circulation pipeline. The circulation pump is installed on the circulation pipeline, and control valves are installed on the circulation pipeline and the guide pipeline respectively. A cutting device for evenly cutting the sea anemones is installed inside the cutting breeding tank. The sea anemones in the temporary holding tank are transported to the cutting breeding tank and cut inside the cutting breeding tank by the circulation pump through the circulation of the temporary holding tank, the circulation pipeline, the guide pipeline, and the cutting breeding tank.

[0006] The cutting device of this invention includes a support frame, a cutting disc, a cutting blade, a tilting motor, and a linear motor. The support frame is fixed to the wall of a cutting and breeding cylinder above the circulation outlet. An inlet / outlet for the cutting disc is provided on the side above the support frame of the cutting and breeding cylinder. A sealing door is installed on the cutting and breeding cylinder at the inlet / outlet. After entering the cutting and breeding cylinder through the inlet / outlet, the cutting disc is placed above the support frame. Multiple placement slots are evenly distributed on the cutting disc, and cross-shaped or straight cutting holes are provided at the bottom of the slots. The cutting blade is located above the cutting disc and includes a cutting frame and a cutting blade. The cutting frame is fixedly connected to the cutting holes. The corresponding cutting blade is placed, and one side of the cutting frame is connected to the output shaft of the flip motor. The base of the flip motor is connected to the slider, and the slider is slidably connected to the slide rail fixed on the inner wall of the cutting breeding tube. The output end of the linear motor is fixedly connected to the slider, and the base of the linear motor is fixed on the inner wall of the cutting breeding tube. The flip motor flips the cutting blade on the side wall of the cutting breeding tube. When cutting is required, the flip motor rotates the cutting blade to a state parallel to the cutting disc. Then, the linear motor drives the slider and the cutting blade to move down, thereby cutting the sea anemone that falls into the placement groove of the cutting disc. A drain pipe is installed at the bottom of the cutting breeding tube, and a drain valve is installed on the drain pipe.

[0007] The present invention describes a method for cutting and breeding tubes. A conveyor belt is installed on the outside of the inlet and outlet of the tube. The rear side of the conveyor belt extends to the inlet of the primary remediation tank. The conveyor belt is fixed on the conveyor frame. Above the conveyor belt, along the conveying direction, a sterilizing liquid spray head and a sterilizing seawater spray head are installed in sequence. The sterilizing liquid spray head and the sterilizing seawater spray head are respectively fixed on the bracket and connected to the sterilizing liquid tube and the sterilizing seawater tube through pipelines. Spray water pumps are installed on the pipelines respectively.

[0008] The present invention describes a temporary holding tank equipped with a temporary holding circulation pump, which enables the liquid to flow within the tank and ensures that the sea anemone is in a flowing state.

[0009] The invention describes a primary circulation pump installed in the primary repair pool. The primary circulation pump enables liquid flow within the primary repair pool, allowing the cut sea anemones to roll and grow within the primary repair pool.

[0010] The present invention installs COD probes on the temporary holding tank and the primary remediation tank to detect the COD concentration in the temporary holding tank and the primary remediation tank.

[0011] The temporary incubation tube of the present invention is equipped with a culture medium inlet, which is connected to a culture medium inlet pipe.

[0012] The temporary holding tank and primary repair pool described in this invention are equipped with adjustable light sources to provide a stable light source for sea anemones.

[0013] The present invention describes a cutting disc with a fixed vibrating motor installed on it, which uses micro-vibration to make the sea anemones fall into the placement groove of the cutting disc.

[0014] A method for propagating sea anemones using a propagation device, characterized by the following steps: (1) Temporary holding: The sea anemone is placed in a temporary holding tube and filled with seawater that has been sterilized and filtered by ultraviolet light. Antibiotics are added to the seawater, and culture medium is added at the same time. The culture medium includes antibiotics, zooxanthellae, and shrimp meat paste. Antibiotics are added for antibacterial effect, zooxanthellae are added to provide energy for the sea anemone, and shrimp meat paste is added to supplement protein, fat, etc. to meet the energy needs after cutting. An adjustable light source is installed on the temporary holding tube and a COD probe is used for detection. When the COD concentration is ≥3 mg / L, the seawater is replaced and the above culture medium is added. (2) Cutting: After 3-5 days of temporary rearing in step (1), the sea anemones will show strong tentacles, fresh color and active feeding, and can be prepared for cutting. When preparing to cut, feeding should be stopped. First, fill the cutting and breeding tube with sterilized seawater, open the control valves on the guide pipe and circulation pipe and turn on the circulation pump. At this time, the water flow direction is temporary rearing tube, guide pipe, cutting and breeding tube, circulation pipe, temporary rearing tube. Keep the water full throughout the process to ensure that the sea anemones will not be stimulated and shrink. During the water circulation process, the sea anemones enter the cutting and breeding tube through the guide pipe and fall onto the cutting plate of the cutting and breeding tube. After the sea anemones are evenly distributed in the placement slot of the cutting plate, keep the water flow for 5-15 minutes. Then turn on the flip motor. The flip motor slowly rotates the cutting blade to a state parallel to the cutting plate. Then the linear motor drives the slider and cutting blade to move down, and then cuts the sea anemones that have fallen into the placement slot of the cutting plate. (3) Restoration and Growth: After cutting, maintain water flow for 20-35 minutes, close the circulation pump, diversion pipeline and control valves on the circulation pipeline, open the bottom drain valve of the cutting breeding tube to completely drain the seawater in the cutting breeding tube, open the sealed door after draining, pull out the cutting disc from the inlet and outlet of the sealed door, place the cut sea anemone on the conveyor belt, and rinse the sea anemone through the sterilization spray head to remove the meat fragments and organic matter, and then rinse it through the sterilization seawater spray head to remove the sterilization solution, and then enter the primary restoration tank. Sterilization seawater and restoration solution are added to the primary restoration tank. The restoration solution includes electrolyzed multivitamins and amino acids, aquatic probiotics and zooxanthellae. Turn on the restoration circulation pump to keep the seawater in the primary circulation tank circulating, so that the cut sea anemone continuously rolls in the primary restoration tank, and detect the COD concentration. When the COD concentration is ≥3 When the concentration is mg / L, replace the seawater and add the above-mentioned repair solution. Adjust the light source to 400-700nm blue light with a light intensity of 1000-3000 lux to provide a stable light source for the sea anemone. Detect and identify sea anemones that show a transparent film or tentacle buds after cutting as dominant growth sea anemones.

[0015] The present invention, by employing the above-described structure and method, has the advantages of simple structure, high reproductive efficiency, and good reproductive effect in sea anemones. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the structure of the present invention.

[0017] Figure 2 yes Figure 1 A schematic diagram of the structure of the cutting propagation tube.

[0018] Figure 3 yes Figure 2 A schematic diagram of the supporting frame structure.

[0019] Figure 4 yes Figure 2 A schematic diagram of the structure of the cutting disc.

[0020] Figure 5 yes Figure 3 Side view. Detailed Implementation

[0021] The present invention will be further described below with reference to the accompanying drawings: As shown in the attached figure, a breeding device for sea anemones is characterized by comprising a support 1, a temporary holding tank 2, a circulation pipeline 3, a guide pipeline 4, a cutting breeding tank 5, and a circulation pump 6. The support 1 is fixed on the ground, and the temporary holding tank 2 and the cutting breeding tank 5 are respectively fixed on the support 1. The guide outlet 8 on the side of the temporary holding tank 2 is connected to the guide inlet 7 above the cutting breeding tank 5 via the guide pipeline 4. The circulation outlet 9 at the bottom of the cutting breeding tank 5 is connected to the circulation inlet 10 above the temporary holding tank 2 via the circulation pipeline 3. The circulation pump 6 is installed on the circulation pipeline 3. Control valves 11 are respectively installed on the circulation pipeline 3 and the guide pipeline 4. The cutting breeding tank 5 is equipped with a cutting device for evenly cutting the sea anemones. The sea anemones in the temporary holding tank 2 are transported to the cutting breeding tank 5 and cut inside the cutting breeding tank 5 by the circulation action of the circulation pump 6.

[0022] Furthermore, the cutting device includes a support frame 12, a cutting disc 13, a cutting blade 14, a tilting motor 15, and a linear motor 16. The support frame 12 is fixed to the wall of the cutting and breeding cylinder 5 above the circulation outlet 9. The cutting and breeding cylinder 5 on the upper side of the support frame 12 is provided with an inlet / outlet 43 for the entry and exit of the cutting disc 13. A sealing door 44 is installed on the cutting and breeding cylinder 5 at the inlet / outlet 43. After the cutting disc 13 enters the cutting and breeding cylinder 5 through the inlet / outlet 43, it is placed above the support frame 12. The cutting disc 13 is provided with a plurality of placement grooves 17 evenly distributed on it. The bottom of the placement grooves 17 is provided with cross-shaped or straight cutting holes 18. The cutting blade 14 is located above the cutting disc 13. The cutting blade 14 includes a cutting frame 19 and a cutting blade 20. The cutting frame 19 is fixedly connected to the cutting blade. The cutting blade 20 corresponding to the hole 18 is connected to the output shaft of the flip motor 15 on one side of the cutting frame 19. The base of the flip motor 15 is connected to the slider 21. The slider 21 is slidably connected to the slide rail 22 fixed on the inner wall of the cutting breeding tube 5. The output end of the linear motor 16 is fixedly connected to the slider 21. The base of the linear motor 16 is fixed on the inner wall of the cutting breeding tube 5. The cutting blade 14 is flipped on the side wall of the cutting breeding tube 5 by the flip motor 15. When cutting is required, the flip motor 15 rotates the cutting blade 14 to a state parallel to the cutting disc 13. Then the linear motor 16 drives the slider 21 and the cutting blade 14 to move down, thereby cutting the sea anemone that falls into the placement groove 17 of the cutting disc 13. A drain pipe 23 is installed at the bottom of the cutting breeding tube 5, and a drain valve 24 is installed on the drain pipe 23.

[0023] Furthermore, a conveyor belt 25 is installed on the outside of the inlet 43 of the cutting and breeding tube 5. The rear side of the conveyor belt 25 extends to the inlet position of the primary repair pool 26. The conveyor belt 25 is fixed on the conveyor frame 27. Above the conveyor belt 25, along the conveying direction, a bactericidal liquid spray head 28 and a bactericidal seawater spray head 29 are installed in sequence. The bactericidal liquid spray head 28 and the bactericidal seawater spray head 29 are respectively fixed on the bracket 1 and connected to the bactericidal liquid tube 30 and the bactericidal seawater tube 31 through pipelines. Spray water pumps 32 are respectively installed on the pipelines.

[0024] Furthermore, a temporary holding circulation pump 33 is installed inside the temporary holding tank 2 to achieve liquid flow inside the temporary holding tank 2 and ensure that the sea anemone is in a flowing state.

[0025] Furthermore, a primary circulation pump 34 is installed in the primary repair pool 26 to achieve liquid flow in the primary repair pool 26, allowing the cut sea anemones to roll and grow within the primary repair pool 26.

[0026] Furthermore, COD probes 35 are installed on the temporary holding tank 2 and the primary remediation tank 26 to detect the COD concentration in the temporary holding tank 2 and the primary remediation tank 26.

[0027] Furthermore, a culture medium inlet is installed on the temporary incubation tube 2, and the culture medium inlet is connected to the culture medium inlet pipe 36.

[0028] Furthermore, adjustable light sources 37 are installed on the temporary holding tank 2 and the primary repair pool 26 to provide a stable light source for the sea anemones.

[0029] Furthermore, a vibration motor 38 is fixedly installed on the cutting disc 13, and the sea anemones are dropped into the placement groove 17 of the cutting disc 13 by micro-vibration.

[0030] The aforementioned sealing door 44 is a door that can close the entrance 43. It is existing technology and closes the entrance 43 through a rubber sealing gasket and a lock body.

[0031] A method for propagating sea anemones using a propagation device, characterized by the following steps: (1) Temporary holding: The sea anemone is placed in the temporary holding tube 2. The temporary holding tube 2 is filled with seawater that has been sterilized by ultraviolet light and filtered for impurities. Antibiotics are added to the seawater, and culture medium is added at the same time. The culture medium includes antibiotics, zooxanthellae, and shrimp meat paste. Antibiotics are added for antibacterial effect, zooxanthellae are added to provide energy source for the sea anemone, and shrimp meat paste is added to supplement protein, fat, etc. to meet the energy demand after cutting. An adjustable light source 37 is installed on the temporary holding tube 2, and a COD probe 35 is used for detection. When the COD concentration is ≥3 mg / L, the seawater is replaced and the above culture medium is added. (2) Cutting: After 3-5 days of temporary rearing in step (1), the sea anemones will show strong tentacles, fresh color, and active feeding, and can be prepared for cutting. When preparing for cutting, feeding should be stopped. First, fill the cutting and breeding tube with sterilized seawater, open the control valve 11 on the guide pipe 4 and the circulation pipe 3 and turn on the circulation pump 6. At this time, the water flow direction is temporary rearing tube 2, guide pipe 4, cutting and breeding tube 5, circulation pipe 3, and temporary rearing tube 2. Keep the tube full of water throughout the process to ensure that the sea anemones will not be stimulated and shrink. During the water circulation process, the sea anemones enter the cutting and breeding tube 5 through the guide pipe 4 and fall onto the cutting plate 13 of the cutting and breeding tube 5. After the sea anemones are evenly distributed in the placement slot 17 of the cutting plate 13, the water flow is maintained for 5-15 minutes. Then, the flip motor 15 is turned on. The flip motor 15 slowly rotates the cutting blades 20 14 to a state parallel to the cutting plate 13. Then, the linear motor 16 drives the slider 21 and the cutting blades 20 14 to move down, thereby cutting the sea anemones that have fallen into the placement slot 17 of the cutting plate 13. (3) Repair and growth: After cutting, maintain water flow for 20-35 minutes, close the control valve 11 on the circulation pump 6, the guide pipe 4, and the circulation pipe 3, open the bottom drain valve 24 of the cutting breeding tube 5 to completely drain the seawater in the cutting breeding tube 5, and after draining, open the sealing door 44, pull out the cutting disc 13 from the inlet 43 of the closed door, place the cut sea anemone on the conveyor belt 25, and rinse the sea anemone through the sterilization spray head 28 to remove the meat scraps and organic matter, and then rinse it through the sterilization seawater spray head 29 to remove the sterilization solution, and then enter the primary repair tank 26. Sterilization seawater and repair solution are added to the primary repair tank 26. The repair solution includes electrolyzed multivitamins, aquatic probiotics, and zooxanthellae. Turn on the repair circulation pump 6 to keep the seawater in the primary circulation tank circulating, so that the cut sea anemone continuously rolls in the primary repair tank 26, and detect the COD concentration. When the COD concentration is ≥3 When the concentration is mg / L, replace the seawater and add the above-mentioned repair solution. Adjust the light source to 400-700nm blue light with a light intensity of 1000-3000 lux to provide a stable light source for the sea anemone. Detect and identify sea anemones that show a transparent film or tentacle buds after cutting as dominant growth sea anemones.

[0032] The antibiotics in the culture medium include one or more of florfenicol, enrofloxacin, and oxytetracycline. Florfenicol is administered at 10-15 mg / kg / day for 3-5 days; enrofloxacin at 10-20 mg / kg / day for 5-7 days; and oxytetracycline at 3-5 ppm for 3-5 days. The final concentration of the zooxanthellae is 100,000-500,000 cells / mL. Shrimp meat paste is fed in small, frequent amounts, at a rate of one soybean per shrimp. If the shrimp is completely coated within 10-15 minutes, feeding can continue. If the shrimp is not completely coated within 30 minutes, it should be removed with a pipette or clamp.

[0033] The aforementioned adjustable light source 37 uses 400-700nm blue light with a light intensity of 1000-3000 lux. Some wavelengths can be absorbed by bacterial endogenous porphyrins and generate free radicals that damage bacteria.

[0034] During the temporary rearing process, 0.1-0.3 ppm of povidone-iodine can be added to the water for prevention. After changing the water, it can be added again, or a 1-6 ppm solution can be used for a 5-10 minute bath. After the bath, the water should be changed in time to kill pathogens such as bacteria, viruses, fungi and protozoa on the surface of the sea anemone and in the aquaculture water.

[0035] The addition of electrolyzed multivitamins and amino acids to the aforementioned repair solution should start with a very low dose and be cautiously increased based on the sea anemone's response, ideally until the sea anemone's condition does not change significantly. The aforementioned aquatic probiotics include Bacillus, photosynthetic bacteria, and lactic acid bacteria. The Bacillus dosage is 1-2 ppm, which powerfully decomposes organic matter (uneaten feed, feces), reduces ammonia nitrogen / nitrite, and produces antibacterial substances that directly inhibit pathogens, thus forming a "protective ring" around the wound. The photosynthetic bacteria dosage is 5-15 ppm, which absorbs and utilizes ammonia nitrogen and sulfur... Toxic substances such as hydrogen sulfide; lactic acid bacteria at 3-5 ppm, which regulate the balance of intestinal and aquatic microorganisms, and produce lactic acid to lower local pH; among the above-mentioned probiotics, Bacillus, photosynthetic bacteria and lactic acid bacteria can be combined in one or more ways to degrade organic matter in water, antagonize pathogenic bacteria and eliminate excessive ammonia nitrogen; zooxanthellae at 100,000-500,000 cells / mL; if the sea anemone is in poor condition, zooxanthellae can be injected into the sea anemone using a micro-syringe, with the injection concentration based on the weight of the zooxanthellae, at 100,000-50,000 cells / g.

[0036] Example 1: The specific implementation method of the propagation equipment for sea anemones is as follows: (1) Temporary holding: Place the sea anemone in temporary holding tank 2, which is filled with seawater that has been sterilized and filtered for impurities by ultraviolet light. Add antibiotics to the seawater. The amount of antibiotics is based on the weight of the sea anemone: florfenicol, 10-15 mg / kg / day, for 3-5 days; enrofloxacin, 10-20 mg / kg / day, for 5-7 days; oxytetracycline, 3-5 ppm, for 3-5 days. The above three antibiotics, florfenicol, enrofloxacin and oxytetracycline, are used simultaneously. Simultaneously, a culture medium is added, which includes antibiotics, zooxanthellae, and shrimp paste. The antibiotics are added for antibacterial purposes, and the zooxanthellae provide an energy source for the sea anemone. After the sea anemone is cut, it will stop feeding for a period of time, at which point the zooxanthellae becomes its sole energy source. The shrimp paste is added to supplement protein and fat to meet its energy needs after cutting. The dosage of antibiotics is based on the weight of the sea anemone: florfenicol, 10-15 mg / kg / day for 3-5 days; enrofloxacin, 10-20 mg / kg / day for 5-7 days; oxytetracycline, 3-5 ppm. Use for 3-5 consecutive days. Florfenicol, enrofloxacin, and oxytetracycline can be used individually, or two or three can be used simultaneously. The final concentration of zooxanthellae should be 100,000-500,000 cells / mL. Feed the shrimp meat paste according to the principle of small, frequent feedings, at a rate of one soybean per shrimp. If the shrimp is completely coated within 10-15 minutes, continue feeding. If it is not finished after 30 minutes, remove it with a pipette or clamp. An adjustable light source 37 is installed on the temporary incubation tank 2, which uses 400-700nm blue light with a light intensity of 1000-3000 lux. Some wavelengths can be absorbed by the endogenous porphyrins of bacteria and generate free radicals that damage the bacteria. COD probe 35 detects the COD concentration. When the COD concentration is too high, ≥3 mg / L, the seawater is replaced at a volume ratio of 1 / 3 to 1 / 2, and the above-mentioned culture medium is added. During the temporary rearing process, 0.1-0.3 ppm of povidone-iodine can be added to the water for prevention. After changing the water, it can be added again, or a 1-6 ppm solution can be used for a 5-10 minute bath. After the bath, the water should be changed in time to kill pathogens such as bacteria, viruses, fungi and protozoa on the surface of the sea anemone and in the aquaculture water.

[0037] (2) Cutting: After 3-5 days of temporary rearing in step (1), the sea anemones will show strong tentacles, fresh color, and active feeding, and can be cut. When preparing to cut, feeding should be stopped. First, fill the cutting and breeding tube 5 with sterilized seawater, open the control valve 11 on the guide pipe 4 and the circulation pipe 3 and turn on the circulation pump 6. At this time, the water flow direction is temporary rearing tube 2, guide pipe 4, cutting and breeding tube 5, circulation pipe 3, and temporary rearing tube 2. Keep the tube full of water throughout the process to ensure that the sea anemones will not be stimulated and shrink. During the water circulation process, the sea anemones enter the cutting and breeding tube 5 through the guide pipe 4 and fall onto the cutting plate 13 of the cutting and breeding tube 5. After the sea anemones are evenly distributed in the placement slot 17 of the cutting plate 13, the water flow is maintained for 5-15 minutes. Then, the flip motor 15 is turned on. The flip motor 15 slowly rotates the cutting blades 20 14 to a state parallel to the cutting plate 13. Then, the linear motor 16 drives the slider 21 and the cutting blades 20 14 to move down, thereby cutting the sea anemones that have fallen into the placement slot 17 of the cutting plate 13. (3) Repair and growth: After cutting, maintain water flow for 20-35 minutes, close the control valve 11 on the circulation pump 6, the guide pipe 4, and the circulation pipe 3, open the bottom drain valve 24 of the cutting breeding tube 5 to completely drain the seawater in the cutting breeding tube 5, and after draining, open the sealing door 44, pull out the cutting disc 13 from the inlet 43 of the closed door, place the cut sea anemone on the conveyor belt 25, and rinse the sea anemone through the sterilization spray head 28 to remove the meat scraps and organic matter, and then rinse it through the sterilization seawater spray head 29 to remove the sterilization solution, and then enter the primary repair tank 26. Sterilization seawater, electrolyzed multivitamins, aquatic probiotics, and zooxanthellae are added to the primary repair tank 26, and the repair circulation pump 6 is turned on to keep the seawater in the primary circulation tank circulating, so that the cut sea anemone continuously rolls in the primary repair tank 26. The addition of the aforementioned electrolyzed multivitamins and amino acids should begin with a very low dose and be cautiously increased based on the sea anemone's response, ideally until the sea anemone's condition does not change significantly. The aforementioned aquatic probiotics include Bacillus, photosynthetic bacteria, and lactic acid bacteria. The Bacillus dosage is 1-2 ppm, which powerfully decomposes organic matter (uneaten feed, feces), reduces ammonia nitrogen / nitrite, and produces antibacterial substances that directly inhibit pathogens, thus forming a "protective ring" around the wound. The photosynthetic bacteria dosage is 5-15 ppm, which absorbs and utilizes toxic substances such as ammonia nitrogen and hydrogen sulfide. Lactic acid bacteria... Acid bacteria at 3-5 ppm regulate the balance of intestinal and aquatic microorganisms, producing lactic acid and other substances to lower local pH. Among the probiotics mentioned above, a combination of Bacillus, photosynthetic bacteria, and lactic acid bacteria is recommended to degrade organic matter in the water, antagonize pathogens, and eliminate excess ammonia nitrogen. Zooxanthin is used at 100,000-500,000 cells / mL. If the sea anemone is in poor condition, zooxanthin can be injected into the sea anemone using a micro-syringe. The injection concentration is based on the weight of the zooxanthin, at 100,000-50,000 cells / g. COD concentration should be monitored. When COD concentration ≥ 3 mg / L, the seawater should be replaced, and the above-mentioned sterilized seawater containing electrolyzed multivitamins, aquatic probiotics, and zooxanthin should be added. The light source should be adjusted (400-700nm blue light, intensity 1000-3000 lux) to provide a stable light source for the sea anemone. Sea anemones exhibiting a transparent film or tentacle buds after being cut are identified as dominant growth sea anemones.

[0038] Example 2 The difference between Example 2 and Example 1 is that in step (1), the antibiotics used during temporary confinement are either a combination of florfenicol and enrofloxacin or a combination of florfenicol and oxytetracycline. Other parameters are the same as in Example 1.

[0039] A water jet pipe 39 is installed inside the cutting and breeding cylinder 5 on the upper side of the cutting disc 13. The water jet pipe 39 extends out of the cutting and breeding cylinder 5 and connects to the water flow conduit 40 and the sterilization spray cylinder 41. A water jet pump 42 is installed on the water flow conduit 40. A sterilization solution, including iodine and antibiotics, is added to the sterilization spray cylinder. The horizontal water flow from the water jet pipe can help the sea anemone fall into the placement tank 17 and can also disinfect the cutting blades 20 14. An infrared counter is installed on the above-mentioned guide pipe 4. The infrared counter detects the number of sea anemones passing through the guide pipe 4. After detecting the number of sea anemones corresponding to the placement tank 17, the valve of the guide pipe 4 is closed.

[0040] Through the combination of the above-mentioned equipment and methods and various embodiments, the survival rate of sea anemones finally reached over 85%.

[0041] The present invention, by employing the above-described structure and method, has the advantages of simple structure, high reproductive efficiency, and good reproductive effect in sea anemones.

Claims

1. A breeding device for sea anemones, characterized in that... The equipment includes a support frame, a temporary holding tank, a circulation pipeline, a guide pipeline, a cutting and breeding tank, and a circulation pump. The support frame is fixed to the ground, and the temporary holding tank and the cutting and breeding tank are fixed on the support frame. The guide outlet on the side of the temporary holding tank is connected to the guide inlet above the cutting and breeding tank via the guide pipeline. The circulation outlet at the bottom of the cutting and breeding tank is connected to the circulation inlet above the temporary holding tank via the circulation pipeline. The circulation pump is installed on the circulation pipeline, and control valves are installed on the circulation pipeline and the guide pipeline respectively. The cutting and breeding tank is equipped with a cutting device that evenly cuts the sea anemones. The equipment also includes the temporary holding tank, the circulation pipeline, the guide pipeline, and the cutting and breeding tank. The anemones in the temporary holding tank are transported to the cutting and breeding tank by a circulation pump and then cut inside the cutting and breeding tank. The cutting device includes a support frame, a cutting disc, cutting blades, a tilting motor, and a linear motor. The support frame is fixed to the wall of the cutting and breeding tank above the circulation outlet. The cutting and breeding tank on the side above the support frame has an inlet and outlet for the cutting disc to enter and exit. A sealing door is installed on the cutting and breeding tank at the inlet and outlet. After entering the cutting and breeding tank through the inlet and outlet, the cutting disc is placed above the support frame. The cutting disc has multiple placement slots evenly distributed on it, and the bottom of the placement slots has a cross-shaped or straight groove. The cutting hole is of the [type] shape, and the cutting tool is located above the cutting disc. The cutting tool includes a cutting frame and cutting blades. Cutting blades corresponding to the positions of the cutting holes are fixedly connected to the cutting frame. One side of the cutting frame is connected to the output shaft of a rotating motor. The base of the rotating motor is connected to a slider, and the slider is slidably connected to a slide rail fixed to the inner wall of the cutting and breeding cylinder. The output end of a linear motor is fixedly connected to the slider, and the base of the linear motor is fixed to the inner wall of the cutting and breeding cylinder. The rotating motor rotates the cutting blades onto the side wall of the cutting and breeding cylinder. When cutting is required, the rotating motor rotates the cutting blades to a state parallel to the cutting disc. Then, the linear motor drives the slider and cutting blade to move down, thereby cutting the sea anemone that falls into the placement groove of the cutting disc. A drain pipe is installed at the bottom of the cutting breeding tube, and a drain valve is installed on the drain pipe. A conveyor belt is installed on the outside of the inlet and outlet of the cutting breeding tube. The rear side of the conveyor belt extends to the inlet position of the primary restoration pool. The conveyor belt is fixed on the conveyor frame. Above the conveyor belt, along the conveying direction, a bactericidal liquid spray head and a bactericidal seawater spray head are installed in sequence. The bactericidal liquid spray head and the bactericidal seawater spray head are fixed on the bracket and connected to the bactericidal liquid tube and the bactericidal seawater tube through pipelines. Spray water pumps are installed on the pipelines respectively.

2. The sea anemone breeding equipment according to claim 1, characterized in that... A temporary holding tank is equipped with a temporary holding circulation pump, which enables the liquid to flow within the tank and ensures that the sea anemone is in a flowing state.

3. The sea anemone breeding equipment according to claim 2, characterized in that... A primary circulation pump is installed in the primary repair pool to achieve liquid flow within the pool, allowing the cut sea anemones to roll and grow within it.

4. The sea anemone breeding equipment according to claim 3, characterized in that... COD probes are installed on the temporary holding tank and the primary remediation tank to detect the COD concentration in the temporary holding tank and the primary remediation tank.

5. A sea anemone breeding device according to claim 4, characterized in that... The temporary incubation tube is equipped with a culture medium inlet, which is connected to the culture medium inlet pipe.

6. The sea anemone breeding device according to claim 5, characterized in that... Adjustable light sources are installed on the temporary holding tank and the primary repair pool to provide a stable light source for the sea anemones.

7. A sea anemone breeding device according to claim 6, characterized in that... A vibration motor is fixedly installed on the cutting disc, and the sea anemones are dropped into the placement groove of the cutting disc by micro-vibration.

8. A method for propagating sea anemones using the propagation equipment described in claim 7, characterized in that... The steps for the breeding method are as follows: (1) Temporary holding: The sea anemone is placed in a temporary holding tube and filled with seawater that has been sterilized and filtered by ultraviolet light. Antibiotics are added to the seawater, and culture medium is added at the same time. The culture medium includes antibiotics, zooxanthellae, and shrimp meat paste. Antibiotics are added for antibacterial effect, zooxanthellae are added to provide energy for the sea anemone, and shrimp meat paste is added to supplement protein and fat to meet the energy needs after cutting. An adjustable light source is installed on the temporary holding tube and a COD probe is used for detection. When the COD concentration is ≥3 mg / L, the seawater is replaced and the above culture medium is added. (2) Cutting: After 3-5 days of temporary rearing in step (1), the sea anemones will show strong tentacles, fresh color and active feeding, ready for cutting. When ready to cut, feeding should be stopped. First, fill the cutting and breeding tube with sterilized seawater, open the control valves on the guide pipe and circulation pipe and turn on the circulation pump. At this time, the water flow direction is temporary rearing tube, guide pipe, cutting and breeding tube, circulation pipe, temporary rearing tube. Keep the water full throughout the process to ensure that the sea anemones will not be stimulated and shrink. During the water circulation process, the sea anemones enter the cutting and breeding tube through the guide pipe and fall onto the cutting plate of the cutting and breeding tube. After the sea anemones are evenly distributed in the placement slot of the cutting plate, keep the water flow for 5-15 minutes. Then turn on the flip motor. The flip motor slowly rotates the cutting blade to a state parallel to the cutting plate. Then the linear motor drives the slider and cutting blade to move down, and then cuts the sea anemones that fall into the placement slot of the cutting plate. (3) Restoration and Growth: After cutting, maintain water flow for 20-35 minutes, close the circulation pump, diversion pipeline and control valves on the circulation pipeline, open the bottom drain valve of the cutting breeding tube to completely drain the seawater in the cutting breeding tube, open the sealed door after draining, pull out the cutting disc from the inlet and outlet of the sealed door, place the cut sea anemone on the conveyor belt, and rinse the sea anemone through the sterilization spray head to remove the meat fragments and organic matter, and then rinse it through the sterilization seawater spray head to remove the sterilization solution, and then enter the primary restoration tank. Sterilization seawater and restoration solution are added to the primary restoration tank. The restoration solution includes electrolyzed multivitamins and amino acids, aquatic probiotics and zooxanthellae. Turn on the restoration circulation pump to keep the seawater in the primary circulation tank circulating, so that the cut sea anemone continuously rolls in the primary restoration tank, and detect the COD concentration. When the COD concentration is ≥3 When the concentration is mg / L, replace the seawater and add the above-mentioned repair solution. Adjust the light source to 400-700nm blue light with a light intensity of 1000-3000 lux to provide a stable light source for the sea anemone. Detect and identify sea anemones that show a transparent film or tentacle buds after cutting as dominant growth sea anemones.

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