Sample multiple detection method and HIV (Human Immunodeficiency Virus) detection kit

Abstract

The invention discloses a multiple sample detection method and an HIV (Human Immunodeficiency Virus) detection kit. The method comprises the following steps: coupling different affinity ligands I which are specifically combined with various substances to be detected to different solid-phase carriers; carrying out sealing treatment on the solid-phase carrier to form a corresponding compound I; coupling different affinity ligands II which are specifically combined with a plurality of objects to be detected to a luminous marker to form a corresponding compound II; mixing a to-be-detected sample with the compound I and the compound II, and if the sample contains at least one of the various to-be-detected substances, combining the to-be-detected substances with the corresponding compound I and the compound II to form a corresponding sandwich compound; enabling the sandwich compound to generate a corresponding light-emitting signal through the excitation substance; and judging the corresponding to-be-detected object according to the difference of the light-emitting signals, and calculating to obtain the concentration of the to-be-detected object. According to the invention, the corresponding to-be-detected object can be judged through different light-emitting signals; meanwhile, the concentration of the substance to be detected can be calculated by calculating the total value of signal values of the whole section of a luminescence curve.

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