A soothing anti-itch composition containing an olive-derived ceramide, uses and skin care

Abstract

The application belongs to the technical field of skin care, and discloses a soothing and itching-relieving composition containing olive-derived ceramide, application and skin care product. The soothing and itching-relieving composition containing olive-derived ceramide comprises olive-derived ceramide, guayule extract and hazelnut oil, and the weight ratio of the olive-derived ceramide, guayule extract and hazelnut oil is 10-30:1-3:1-20. The application provides a soothing and itching-relieving composition containing olive-derived ceramide. The olive-derived ceramide, guayule extract and hazelnut oil are compounded, synergistic effect is generated, the soothing effect of the composition is effectively improved, and the effect of soothing and itching-relieving is achieved.

Description

Technical Field

[0001] This application relates to the field of skin care technology, specifically to a soothing and antipruritic composition containing olive-derived ceramides, its uses, and skin care products. Background Technology

[0002] There is a close relationship between skin soothing and itch relief: soothing is the physiological basis for itch relief, while itch relief is a direct manifestation of soothing. When the skin barrier is damaged due to dryness, inflammation, or external stimuli, nerve endings release itching signals. At this time, repairing the barrier through soothing methods such as moisturizing and anti-inflammation can effectively break the vicious cycle of itching.

[0003] Prior art 1: Chinese patent application 202010674230.1 discloses a scalp care composition and its application. By weight percentage, the scalp care composition comprises: a scalp barrier function repair component of 0.1-2%, comprising 0.1-0.5% water-soluble ceramide, 0.1-0.5% Lactobacillus / soybean fermentation product filtrate, and 0.2-0.8% Sargassum fusiforme extract; an oil-controlling component of 0.1-1%, comprising 0.1-0.5% Uncaria rhynchophylla extract and 0.1-0.5% Magnolia officinalis bark extract; and an anti-allergic component of 0.1-0.5%, comprising 0.1-0.5% maltodextrin. This scalp care composition is mild and safe, with low irritation, effectively improving the scalp microbial environment, and has the effects of balancing oil and water, soothing and relieving itching, and repairing the damaged scalp barrier.

[0004] Existing technology 1 uses a combination of scalp barrier repair components and anti-allergy components to effectively soothe and relieve scalp itching, which is a technology worth learning from. However, the scalp is somewhat different from the skin on other parts of the body. Due to the large number of hair follicles on the scalp, it needs to control oil production, which limits the use of oily ingredients. On the other hand, the skin on the face or hands tends to use oily ingredients to enhance lipid affinity, barrier repair ability, and water retention.

[0005] Therefore, the composition of the prior art 1 is not suitable for use on the skin such as the face, and it is necessary to develop a soothing and antipruritic composition suitable for use on the skin of the face and other areas. Summary of the Invention

[0006] One of the objectives of this application is to provide a soothing and antipruritic composition containing olive-derived ceramides, which is formulated with a variety of oil-soluble ingredients and has excellent soothing and antipruritic effects. Due to its oil-soluble properties, it is more suitable for use on the skin of the face and other areas.

[0007] Another objective of this application is to provide the use of a soothing and antipruritic composition containing olive-derived ceramides, wherein skin care products prepared using this soothing and antipruritic composition containing olive-derived ceramides can provide skin soothing and antipruritic effects.

[0008] In addition, this application also provides a skin care product containing the soothing and antipruritic composition of olive-derived ceramides of this application, which has the effect of soothing and relieving skin itch.

[0009] To achieve the above objectives, this application provides a soothing and antipruritic composition containing olive-derived ceramides, comprising olive-derived ceramides, white immortelle extract, and hazelnut oil, wherein the weight ratio of the olive-derived ceramides, white immortelle extract, and hazelnut oil is 10-30:1-3:1-20.

[0010] Preferably, the composition further includes ascorbic acid tetraisopalmitate, wherein the weight ratio of the olive-derived ceramide, white immortelle extract, hazelnut oil, and ascorbic acid tetraisopalmitate is 10-30:1-3:1-20:1-3. This application also provides the use of the above-described soothing and antipruritic composition containing olive-derived ceramide in the preparation of skin care products.

[0011] Furthermore, this application also discloses a skin care product containing 0.01-2 wt% of the soothing and antipruritic composition containing olive-derived ceramides.

[0012] Beneficial effects

[0013] Compared with the prior art, this application provides a soothing and antipruritic composition containing olive-derived ceramides. The combination of olive-derived ceramides, white immortelle extract and hazelnut oil produces a synergistic effect, effectively enhancing the soothing efficacy of the composition, thereby achieving the effect of soothing and relieving itching. Detailed Implementation

[0014] The present application will be further described below with reference to embodiments, but this does not constitute any limitation on the present application. Any limited modifications made within the scope of the claims of the present application shall still be within the scope of the claims of the present application.

[0015] To illustrate the technical content of this application in detail, the following description is provided in conjunction with the embodiments.

[0016] In the following examples and comparative examples, the olive-derived ceramide was purchased from Luxinno (Guangzhou) Biotechnology Co., Ltd., containing 1% olive-derived ceramide NP; the white immortelle extract was purchased from PERFELINE®-ESSENCE provided by Guangzhou Aoxue Chemical Co., Ltd., containing 98wt% caprylic / capric triglycerides and 2% white immortelle flower extract; the Italian immortelle oil was purchased from Xiteng (Shanghai) International Trading Co., Ltd.; the globe amaranth extract was purchased from Jiafashi (Shanghai) Trading Co., Ltd., containing 97wt% caprylic / capric triglycerides and 3wt% globe amaranth extract; the macadamia nut oil was purchased from Guangzhou Nuoran Biotechnology Co., Ltd., with a content of 100%; and the hazelnut oil was purchased from Guangzhou Minghui Fine Chemical Co., Ltd., with a content of 100%.

[0017] In the following embodiments and comparative examples, unless otherwise specified, the parts are parts by weight and the percentage is a weight percentage.

[0018] It should be noted that in the following examples and comparative examples, the component amounts are calculated by pure substance weight. For example, 2 parts of white-leaf helichrysum extract is equivalent to 2 parts by weight of white-leaf helichrysum extract and 98 parts of caprylic / capric triglycerides, which together form PERFELINE®-ESSENCE.

[0019] Example 1

[0020] A soothing and antipruritic composition comprising 10 parts olive-derived ceramide, 3 parts white immortelle extract and 20 parts hazelnut oil.

[0021] Example 2

[0022] A soothing and antipruritic composition comprising 30 parts olive-derived ceramide, 1 part white immortelle extract and 1 part hazelnut oil.

[0023] Example 3

[0024] A soothing and antipruritic composition comprising 20 parts olive-derived ceramide, 2 parts white immortelle extract and 8 parts hazelnut oil.

[0025] Example 4

[0026] A soothing and antipruritic composition comprising 20 parts olive-derived ceramide, 2 parts white immortelle extract, 8 parts hazelnut oil and 2 parts ascorbate tetraisopalmitate.

[0027] Comparative Example 1

[0028] A soothing and antipruritic composition comprising 6 parts white immortelle extract and 24 parts hazelnut oil.

[0029] Comparative Example 2

[0030] A soothing and antipruritic composition comprising 21.4 parts olive-derived ceramide and 8.6 parts hazelnut oil.

[0031] Comparative Example 3

[0032] A soothing and antipruritic composition comprising 27.3 parts olive-derived ceramide and 2.7 parts white immortelle extract.

[0033] Comparative Example 4

[0034] A soothing and antipruritic composition comprising 20 parts olive-derived ceramide, 2 parts Italian immortelle oil, and 8 parts hazelnut oil.

[0035] Comparative Example 5

[0036] A soothing and antipruritic composition comprising 20 parts olive-derived ceramide, 2 parts chamomile extract and 8 parts hazelnut oil.

[0037] Comparative Example 6

[0038] A soothing and antipruritic composition comprising 20 parts macadamia nut oil, 2 parts white immortelle extract and 8 parts hazelnut oil.

[0039] Comparative Example 7

[0040] A soothing and antipruritic composition comprising 20 parts olive-derived ceramide, 2 parts white immortelle extract and 8 parts macadamia oil.

[0041] Comparative Example 8

[0042] Macadamia nut oil

[0043] Comparative Example 9

[0044] Italian Helichrysum Oil

[0045] Comparative Example 10

[0046] White immortelle extract

[0047] Comparative Example 11

[0048] Hazelnut oil

[0049] Comparative Example 12

[0050] Ceramides from olives

[0051] Comparative Example 13

[0052] Globe Amaranth Extract

[0053] Soothing efficacy test

[0054] Experimental Objectives and Principles

[0055] Hyaluronidase is a specific lysin that breaks down hyaluronic acid. It is involved in allergic reactions and is strongly correlated with the release of histamine from mast cells. (Test sample)

[0056] Whether a substance has a soothing effect can be determined by the hyaluronidase inhibition rate. The higher the hyaluronidase inhibition rate, the stronger the soothing effect of the substance, and vice versa.

[0057] This experiment references the laboratory method (HMC-WI-029 hyaluronidase inhibition rate). The results of the hyaluronidase inhibition rate test of the test sample and the negative control were compared. If the inhibition rate of the test sample was higher than that of the negative control and the difference was statistically significant (P<0.05), the test sample could be considered to have a soothing effect.

[0058] 2. Test Indicators

[0059] Table 1 Test Indicators

[0060] *If the hyaluronidase inhibition rate of the sample is significantly higher than that of the negative control (P < 0.5), the sample can be considered to have a soothing effect.

[0061] 3. Experimental Materials and Methods

[0062] 3.1 Instruments and Equipment

[0063] BASA224S Analytical Balance

[0064] L6S UV spectrophotometer

[0065] 3.2 Reagents

[0066] Hyaluronidase

[0067] Sodium hyaluronate

[0068] 3.3 Experimental Methods

[0069] Treatment of control materials and test samples

[0070] Sample Groups: Examples and Comparative Examples

[0071] Positive control (dipotassium glycyrrhizate, purity ≥98%): Dilute with pure water to a concentration of 3%.

[0072] Negative control: Anhydrous ethanol

[0073] Test operation procedures

[0074] Set up a sample group, a sample background group, a solvent group, and a solvent background group, with three replicates for each group. Add different reagent solutions to each of the four groups, shake well, and incubate at room temperature for 30 minutes for color development. Measure the absorbance at 528 nm using a UV spectrophotometer.

[0075] Calculation formula

[0076]

[0077] In the formula: A—is the absorbance of the reaction solution without the sample; B—is the absorbance of the reaction solution without the sample and enzyme.

[0078] C—Absorbance of the reaction solution containing the sample and enzyme; D—Absorbance of the reaction solution containing the sample and without the enzyme.

[0079] Data Analysis

[0080] Examples 1-4 and Comparative Examples 1-13 were tested according to the above method, and the results are shown in Table 2.

[0081] Table 2. Results of the soothing effects of Examples 1-4 and Comparative Examples 1-13

[0082] Example 1 56.34% Example 2 53.77% Example 3 50.81% Example 4 61.47% Comparative Example 1 36.78% Comparative Example 2 33.43% Comparative Example 3 35.59% Comparative Example 4 32.18% Comparative Example 5 29.69% Comparative Example 6 28.43% Comparative Example 7 27.15% Comparative Example 8 26.82% Comparative Example 9 25.43% Comparative Example 10 28.63% Comparative Example 11 38.77% Comparative Example 12 42.39% Comparative Example 13 39.17%

[0083] According to the results in Table 1:

[0084] Based on the data from Comparative Examples 8 to 13, when using pure raw materials to test the hyaluronidase inhibition rate, the order of soothing efficacy among the raw materials should be: ceramide from olives > chamomile extract > hazelnut oil > white immortelle extract > macadamia nut oil > Italian immortelle oil.

[0085] Comparing the data from Example 3 and Comparative Examples 1-3, it can be seen that when any two of the following are combined: olive-derived ceramide, white immortelle extract, and hazelnut oil, there is no significant increase in hyaluronidase inhibition rate compared to using a single component. This is because the concentration of each component is reduced when any two are combined. Therefore, it can be determined that the combination of any two of the following can only produce a simple additive effect. Furthermore, based on the data comparison of Comparative Examples 11, 12, and 2, it can be inferred that the combination of olive-derived ceramide and hazelnut oil may also produce a low antagonistic effect. However, when olive-derived ceramide, white immortelle extract, and hazelnut oil are combined in Example 3 of this application, the hyaluronidase inhibition rate of the composition is significantly improved. This indicates that the combination of olive-derived ceramide, white immortelle extract, and hazelnut oil in this application produces a synergistic effect, which can significantly improve the hyaluronidase inhibition rate of the composition.

[0086] According to the data from Comparative Examples 4-7, when different components were used to replace one of the olive-derived ceramides, white immortelle extract, and hazelnut oil, the hyaluronidase inhibition rate of the technical solution of this application could not be achieved, further verifying that the technical solution of this application has a synergistic effect.

[0087] Antipruritic efficacy test

[0088] Experimental Objective

[0089] This experiment aims to evaluate the inhibitory effect of the test sample on the activation of pruritus-related cells and the release of pruritus mediators using an in vitro cell model, thereby making a preliminary judgment on its antipruritic effect and providing a scientific basis for subsequent in vivo experiments and product development.

[0090] Experimental Principle

[0091] The occurrence of pruritus is closely related to the activation of mast cells and the release of histamine, inflammatory factors (such as IL-6 and TNF-α), and the activation of pruritus-related receptors (such as TRPV1) on sensory neurons. This experiment used mast cells (such as RBL-2H3 cells) as a model, induced cell activation with a specific stimulant (Compound 48 / 80), and examined the effect of the test sample on the amount of histamine released by the cells to reflect its antipruritic potential.

[0092] 3. Experimental Materials and Instruments

[0093] 3.1 Cell lines

[0094] Mast cell line: RBL-2H3 cells

[0095] 3.2 Main Reagents

[0096] Cell culture media: RPMI-1640 medium (RBL-2H3 cells) and DMEM medium (ND7 / 23 cells), both supplemented with 10% fetal bovine serum (FBS), 100 U / mL penicillin, and 100 μg / mL streptomycin.

[0097] Stimulant: Compound 48 / 80 (mast cell activator, Sigma-Aldrich)

[0098] Test kit: Histamine ELISA test kit (Thermo Fisher Scientific)

[0099] Other reagents: PBS buffer, trypsin-EDTA digestion solution, DMSO (solvent control)

[0100] Test sample

[0101] 3.3 Main Instruments

[0102] Cell incubator

[0103] Clean bench

[0104] ELISA reader

[0105] high-speed centrifuge

[0106] Inverted microscope

[0107] pipette

[0108] 96-well cell culture plate

[0109] 4 Experimental Methods

[0110] 4.1 Cell Culture and Preparation

[0111] RBL-2H3 cell culture: Cells were seeded in RPMI-1640 medium containing 10% FBS and cultured in a 37°C, 5% CO2 incubator. When the cell confluence reached 80%-90%, the cells were passaged by digestion with 0.25% trypsin-EDTA. Logarithmic growth phase cells were selected for the experiment.

[0112] 2. Cell seeding: Seed the above cells at an appropriate density into 96-well plates (for ELISA detection) and culture for 24 hours to allow the cells to adhere.

[0113] 4.2 Experimental Grouping

[0114] Blank control group (Control): Only cell culture medium was added, without adding stimulants or test samples;

[0115] Positive control group (Model): Cell culture medium and stimulant were added, but no test sample was added;

[0116] Sample group: Add cell culture medium, stimulant and 1 μg / mL of the sample to be tested;

[0117] 4.3 Sample preparation and stimulation

[0118] After cell adhesion, the original culture medium was aspirated. The sample groups were given culture medium containing 1 μg / mL of the test sample, while the blank control group and positive control group were given the corresponding culture medium. The cells were incubated for 2 hours. Subsequently, except for the blank control group, the other groups were given stimulants: RBL-2H3 cells were given 1 μg / mL of Compound 48 / 80.

[0119] 4.4 Detection Indicators and Methods

[0120] 4.4.1 Histamine release assay (RBL-2H3 cells)

[0121] After stimulation incubation, cell supernatant was collected from each well. The absorbance at 450 nm was measured using a microplate reader according to the instructions of the histamine ELISA kit. The concentration of histamine in the supernatant was calculated based on the standard curve, and the differences in histamine release among the groups were compared.

[0122] Data Analysis

[0123] Measurement data are expressed as mean ± standard deviation (x ± s), and each experiment was repeated at least three times. The inhibition rate of the sample for each index was calculated, and the inhibition rate was used to evaluate the antipruritic effect of the test sample.

[0124] Inhibition rate = [(BC) / (BA)] * 100%

[0125] A: The blank control group consisted of mast cell culture systems without any stimulation, with only basal histamine release being measured.

[0126] B: The positive control group only received histamine release stimulant (Compound 48 / 80), representing the maximum histamine release.

[0127] C: The group where both the stimulant and the test sample were added represents the histamine release level after intervention with the test sample.

[0128] The above methods were tested on Examples 1-4 and Comparative Examples 1-13, and the results are shown in Table 3.

[0129] Table 3. Results of antipruritic efficacy of Examples 1-4 and Comparative Examples 1-13

[0130] Example 1 49.77% Example 2 45.83% Example 3 43.19% Example 4 51.89% Comparative Example 1 26.19% Comparative Example 2 23.47% Comparative Example 3 21.03% Comparative Example 4 19.88% Comparative Example 5 19.05% Comparative Example 6 17.62% Comparative Example 7 17.43% Comparative Example 8 10.84% Comparative Example 9 21.37% Comparative Example 10 25.31% Comparative Example 11 19.37% Comparative Example 12 18.64% Comparative Example 13 30.12%

[0131] According to the results in Table 3:

[0132] According to the data from Examples 1-4, the technical solution of this application has a superior antipruritic effect on the skin. In Example 4, ascorbic acid tetraisopalmitate was added to the basis of olive-derived ceramide, white immortelle extract and hazelnut oil, which significantly increased the antipruritic effect on the skin.

[0133] According to the data from Comparative Examples 8-13, the antipruritic effects of the single components were ranked as follows: Globe amaranth extract > White immortelle extract > Italian immortelle oil > Hazelnut oil > Olive-derived ceramide > Macadamia nut oil. Furthermore, the antipruritic effect of globe amaranth extract itself was very significant, reaching as high as 30%.

[0134] Comparing the data from Example 3 and Comparative Examples 1-3, it can be seen that when Comparative Example 1 uses a combination of white immortelle extract and hazelnut oil, the antipruritic effect is improved to a certain extent compared to using a single component, but the improvement is not significant. This indicates that the combination of white immortelle extract and hazelnut oil only produces a simple additive effect. However, when Comparative Example 2 uses a combination of hazelnut oil and olive-derived ceramides, its antipruritic effect is significantly improved compared to a single component. This suggests that the combination of hazelnut oil and olive-derived ceramides has a certain degree of synergistic effect in improving the antipruritic effect of the composition, but the synergistic effect is weak, and its antipruritic effect is significantly reduced compared to Example 3 of this application. Comparative Example 3 also only shows a simple additive effect. Therefore, it can be inferred that Example 3 of this application has a significant synergistic effect, which can significantly improve the antipruritic effect of the composition, and this synergistic effect is formed by adding white immortelle extract to the combination of hazelnut oil and olive-derived ceramides.

[0135] Based on the above speculation, this application investigated the necessity of adding Helichrysum fusiforme extract through Comparative Examples 4 and 5. According to the data comparison between Example 3 and Comparative Examples 4 and 5, it was found that both Helichrysum fusiforme oil and Helichrysum fusiforme extract significantly weakened the synergistic effect formed by the combination of hazelnut oil and olive-derived ceramides. The reason may be that Helichrysum fusiforme oil or Helichrysum fusiforme extract reduced the concentration of hazelnut oil and olive-derived ceramides, thereby causing their weak synergistic effect to disappear. However, the addition of Helichrysum fusiforme extract to the combination of hazelnut oil and olive-derived ceramides in this application can significantly improve the antipruritic effect of the composition.

[0136] Similarly, to verify the necessity of hazelnut oil and olive-derived ceramides in the technical solution of this application, this application used macadamia nut oil to replace hazelnut oil and olive-derived ceramides in Comparative Examples 6 and 7, respectively. The results showed that the technical solution of this application can only achieve significant antipruritic effects by combining olive-derived ceramides, white immortelle extract and hazelnut oil.

[0137] The embodiments presented herein are merely selected implementations based on combinations of all possible embodiments. The appended claims should not be limited to the embodiments described herein. Some numerical ranges used in the claims include sub-ranges within them, and variations within these ranges should also be covered by the appended claims.

Claims

1. A soothing and antipruritic composition containing olive-derived ceramides, characterized in that, It includes olive-derived ceramides, white immortelle extract, hazelnut oil, and ascorbate tetraisopalmitate, wherein the weight ratio of the olive-derived ceramides, white immortelle extract, hazelnut oil, and ascorbate tetraisopalmitate is 10-30:1-3:1-20:1-3.

2. Use of the soothing and antipruritic composition containing olive-derived ceramides as described in claim 1 to prepare skin care products.

3. A skincare product, characterized in that, A soothing and antipruritic composition containing olive-derived ceramides as described in claim 1, comprising 0.01-2 wt%.

Images