Primer combination and method for LAMP detection of Candidatus Liberibacter asiaticum
By designing primer combinations targeting the s9 gene, a pathogen-related gene of citrus Huanglongbing (HLB), and combining them with the LAMP detection method, a rapid and accurate detection of HLB was achieved. This method overcomes the shortcomings of existing detection methods and is suitable for the prevention and control of HLB.
CN121406804APending Publication Date: 2026-01-27INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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Patent Information
- Application Number
- CN202511015206.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Priority Date
- 2024-07-26
- Filing Date
- 2025-07-23
- Publication Date
- 2026-01-27
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Figure CN121406804A_ABST
Abstract
The invention belongs to the technical field of pathogenic bacteria detection, and particularly relates to a primer combination and a method for liberobacter asiaticum LAMP detection. The invention provides a primer combination for LAMP (Loop-Mediated Isothermal Amplification) detection of Candidatus Liberibacter asiaticum, wherein the primer combination comprises F3-05-yq, B3-05-yq, FIP-05-yq, BIP-05-yq and LB-05-yq. The invention also provides a method for carrying out liberobacter asiaticum LAMP detection by using the primer combination. The method can rapidly and accurately detect the Candidatus Liberobacter asiaticum in the sample, the detection cost is low, the detection result is visual, and the method has a wide application prospect in prevention and control of the Candidatus Liberobacter asiaticum.
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