A tricholoma matsutake endophytic fungus fermentation oil with oil control and water supplementing effects, and a preparation method and application thereof

Abstract

This invention relates to a matsutake mushroom endophytic fungal fermented oil with oil-controlling and water-replenishing effects, its preparation method, and its application. The preparation method includes: (1) inoculating matsutake mushroom endophytic fungal seed liquid into a fermentation medium for fermentation culture to obtain a primary fermentation broth; (2) mixing the primary fermentation broth with vegetable oil and a linolenic acid-glycerol-based ternary eutectic solvent, continuing fermentation culture, and collecting the upper oil phase by centrifugation to obtain matsutake mushroom endophytic fungal fermented oil. The matsutake mushroom endophytic fungal fermented oil prepared by this invention is rich in water-soluble and oil-soluble active ingredients and has excellent water-oil balance regulating ability.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, specifically relating to a fermented oil of matsutake mushroom endophytic fungi with oil-controlling and moisturizing effects, its preparation method, and its application. Background Technology

[0002] Matsutake mushrooms are a precious medicinal and edible fungus, mainly distributed in temperate and subtropical high-altitude forest ecosystems. They rely on forming a symbiotic relationship with the roots of specific tree species to grow. Matsutake mushrooms contain dozens of active nutrients and are rich in protein and carbohydrate resources.

[0003] Studies have confirmed that matsutake mushrooms are rich in a variety of active substances with important physiological functions, among which polysaccharides and flavonoids are two core functional components: matsutake polysaccharides, as natural high-molecular-weight carbohydrates, have significant immunomodulatory and antioxidant biological activities; matsutake flavonoids are a class of secondary metabolites containing benzopyran ring structures, which have strong free radical scavenging capabilities and excellent moisturizing effects.

[0004] Currently, the acquisition of matsutake polysaccharides and flavonoids mainly relies on direct extraction from the fruiting body of matsutake mushrooms. However, matsutake mushrooms have extremely demanding requirements for their growth environment, making large-scale artificial cultivation impossible. Furthermore, the slow growth and metabolic focus of matsutake fruiting bodies on structural substances and secondary metabolism result in low synthesis efficiency of flavonoids and polysaccharides. In addition, water-soluble and fat-soluble active ingredients in the extracts are difficult to coexist, significantly reducing the utilization rate of matsutake mushrooms.

[0005] Endophytic fungi of matsutake mushrooms are a group of microorganisms that have long coexisted with matsutake mushrooms. Through long-term co-evolution, their metabolic pathways are somewhat similar to those of matsutake mushrooms, and they possess the potential to synthesize the characteristic active ingredients of matsutake mushrooms. Compared with natural matsutake fruiting bodies, matsutake endophytic fungi have advantages such as faster growth rate, milder culture conditions, and the ability to achieve large-scale production through fermentation engineering. Furthermore, by optimizing fermentation process parameters, their metabolic flow can be directionally regulated.

[0006] Therefore, developing a fermentation process for matsutake mushroom endophytic fungi to achieve targeted and efficient enrichment of matsutake polysaccharides and flavonoids, and to improve the water-oil balance regulation ability of fermentation products, has become one of the urgent technical problems to be solved. Summary of the Invention

[0007] To address the shortcomings of existing technologies, the present invention aims to provide a fermented oil of matsutake endophytic fungi with oil-controlling and water-replenishing effects, its preparation method, and its application.

[0008] To achieve this objective, the present invention adopts the following technical solution:

[0009] In a first aspect, the present invention provides a method for preparing fermented oil from matsutake mushroom endophytic fungi, the method comprising:

[0010] (1) The seed liquid of matsutake endophytic fungi was inoculated into a fermentation medium for fermentation culture to obtain a primary fermentation broth;

[0011] (2) The primary fermentation broth was mixed with vegetable oil and linolenic acid-glycerol ternary eutectic solvent, and fermentation was continued. The upper oil phase was collected by centrifugation to obtain matsutake endophytic fungal fermentation oil.

[0012] This invention uses matsutake endophytic fungi as a highly efficient substitute for matsutake, and creatively utilizes vegetable oil and linolenic acid-glycerol ternary eutectic solvent to further ferment the primary fermentation broth of matsutake endophytic fungi.

[0013] First, as a highly effective alternative to matsutake mushrooms, the endophytic fungi of matsutake mushrooms produce polysaccharides, flavonoids, and a large number of other water-soluble and oil-soluble active ingredients during the fermentation process, which gives the fermented products excellent water-oil balance regulation capabilities.

[0014] Secondly, the fermentation raw materials of this invention include vegetable oil and linolenic acid, which can be used to synthesize and enrich flavonoids, the characteristic fat-soluble components of matsutake mushrooms, thereby increasing the content of flavonoids in the product.

[0015] In addition, the linolenic acid-glycerol-based ternary eutectic solvent self-assembles into water-in-oil vesicles during fermentation, which can improve the transdermal absorption of water-soluble active ingredients in the fermentation broth. At the same time, the addition of glycerol significantly improves the stability of water-in-oil vesicles and increases the encapsulation rate of water-soluble active ingredients in oils. Furthermore, as a highly efficient hydrating ingredient, the eutectic solvent prepared from glycerol can further enhance the water-oil balance regulation ability of fermented oils.

[0016] Preferably, the hydrogen bond donor of the linolenic acid-glycerol-based ternary eutectic solvent in step (2) is selected from linolenic acid and glycerol.

[0017] Preferably, the hydrogen bond acceptor of the linolenic acid-glycerol ternary eutectic solvent in step (2) is selected from any one or a combination of at least two of lauric acid, decanoic acid, stearic acid, coconut oil acid or palmitic acid.

[0018] Studies have found that the eutectic solvents obtained by any combination of the above-mentioned hydrogen bond acceptors and hydrogen bond donors produce fermentation oils with varying water replenishment and oil control effects. The following specific types of eutectic solvents are preferred, as they further enhance the water replenishment and oil control effects of fermentation oils.

[0019] Preferably, the linolenic acid-glycerol-based ternary eutectic solvent in step (2) is obtained by reacting linolenic acid, glycerol and coconut oil acid at 50-90℃ (e.g., 50℃, 60℃, 70℃, 80℃, 90℃, etc.).

[0020] Furthermore, by using linolenic acid and glycerol as hydrogen bond donors and coconut oil acid as hydrogen bond acceptor, the prepared ternary eutectic solvent can better regulate the metabolism of endophytic fungi in matsutake mushrooms and improve the water-oil balance regulation ability of the product.

[0021] Preferably, the molar ratio of linolenic acid, glycerol and coconut oil acid is (5-10):(1-5):(1-5).

[0022] Among them, the specific point values ​​for 5-10 can be 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10, etc., and the specific point values ​​for 1-5 can be 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, etc.

[0023] Preferably, the vegetable oil in step (2) includes any one or a combination of at least two of the following: babassu seed oil, meadowfoam seed oil, jojoba seed oil, linseed oil, camellia oil, safflower seed oil, or perilla seed oil.

[0024] Preferably, the vegetable oil in step (2) includes a combination of meadowfoam seed oil, flaxseed oil and camellia oil.

[0025] This invention creatively discovers that the combination of meadowfoam seed oil, flaxseed oil, and camellia oil in the preparation of fermented oil from matsutake mushroom endophytic fungi can better improve the water replenishment and oil control effect of the fermented oil. The three have a significant synergistic effect in improving the oil control and water replenishment effect of the product.

[0026] Preferably, the mass ratio of meadowfoam seed oil, flaxseed oil and camellia oil is (1-3):(1-3):(1-3).

[0027] Among them, the specific point values ​​in 1-3 can be selected as 1, 1.3, 1.6, 1.9, 2.2, 2.5, 2.8, 3, etc.

[0028] Preferably, the mass ratio of the primary fermentation broth, vegetable oil, and linolenic acid-glycerol-based ternary eutectic solvent in step (2) is (30-60):(40-60):(1-10).

[0029] Among them, the specific point values ​​in the range of 30-60 can be 30, 35, 40, 45, 50, 55, 60, etc.; the specific point values ​​in the range of 40-60 can be 40, 45, 50, 55, 60, etc.; and the specific point values ​​in the range of 1-10 can be 1, 3, 5, 7, 9, 10, etc.

[0030] Preferably, the matsutake endophytic fungus in the matsutake endophytic fungus seed liquid in step (1) is the matsutake endophytic fungus strain XWJ0001.

[0031] The endophytic fungus strain XWJ0001 of the matsutake mushroom is classified as Umbelopsis sp., with accession number CGMCCNo.42463, accession date of December 4, 2025, and deposited at the China General Microbiological Culture Collection Center (CGMCC) at No. 3, No. 1 Beichen West Road, Chaoyang District, Beijing.

[0032] Preferably, the inoculation volume of the matsutake endophytic fungal seed liquid in step (1) is 1-10% of the fermentation medium volume, for example, it can be 1%, 3%, 5%, 7%, 9%, 10%, etc.

[0033] Preferably, the fermentation medium in step (1) comprises: yeast powder, peptone, glycerol, potassium dihydrogen phosphate, magnesium sulfate and water.

[0034] Preferably, the fermentation culture medium in step (1) comprises, by mass percentage: 0.5-2% yeast powder, 1-5% peptone, 1-5% glycerol, 0.1-0.3% potassium dihydrogen phosphate, 0.1-0.3% magnesium sulfate, and the remainder water.

[0035] Among them, the specific point values ​​in the 0.5-2% range can be 0.5%, 1%, 1.5%, 2%, etc.; the specific point values ​​in the 1-5% range can be 1%, 2%, 3%, 4%, 5%, etc.; and the specific point values ​​in the 0.1-0.3% range can be 0.1%, 0.15%, 0.2%, 0.25%, 0.3%, etc.

[0036] Preferably, the fermentation temperature in step (1) is 25-35℃ (e.g., 25℃, 27℃, 29℃, 31℃, 33℃, 35℃, etc.), and the fermentation time is 6-24 h (e.g., 6 h, 10 h, 14 h, 18 h, 22 h, 24 h, etc.).

[0037] Preferably, the fermentation culture in step (1) is carried out under agitation conditions with an aeration rate (e.g., 40 L, 50 L, 60 L, 70 L, 80 L, etc.), a pressure of 0.05-0.3 MPa (e.g., 0.05 MPa, 0.1 MPa, 0.15 MPa, 0.2 MPa, 0.25 MPa, 0.3 MPa, etc.), and a speed of 50-150 rpm (e.g., 50 rpm, 80 rpm, 110 rpm, 130 rpm, 150 rpm, etc.).

[0038] Preferably, the preparation of the matsutake endophytic fungal seed liquid in step (1) can be obtained using existing techniques in the art, for example, by the following process:

[0039] The endophytic fungal strain of matsutake mushroom was inoculated into YPD medium and cultured to the logarithmic phase to obtain seed culture.

[0040] Preferably, the fermentation temperature in step (2) is 25-35℃ (e.g., 25℃, 27℃, 29℃, 31℃, 33℃, 35℃, etc.), and the fermentation time is 6-24 h (e.g., 6 h, 10 h, 14 h, 18 h, 22 h, 24 h, etc.).

[0041] In a second aspect, the present invention provides a matsutake mushroom endophytic fungal fermentation oil prepared by the preparation method described in the first aspect.

[0042] Thirdly, the present invention provides the application of matsutake endophytic fungal fermented oil as described in the second aspect in cosmetics.

[0043] Preferably, the cosmetic product has oil-controlling and moisturizing effects.

[0044] Compared with the prior art, the present invention has the following beneficial effects:

[0045] This invention uses matsutake endophytic fungi as a highly efficient substitute for matsutake, and creatively utilizes vegetable oil and linolenic acid-glycerol ternary eutectic solvent to ferment matsutake endophytic fungi.

[0046] First, as a highly effective alternative to matsutake mushrooms, the endophytic fungi of matsutake mushrooms produce polysaccharides, flavonoids, and a large number of other water-soluble and oil-soluble active ingredients during the fermentation process, which gives the fermented products excellent water-oil balance regulation capabilities.

[0047] Secondly, the fermentation raw materials of this invention include vegetable oil and linolenic acid, which can be used to synthesize and enrich flavonoids, the characteristic fat-soluble components of matsutake mushrooms, thereby increasing the content of flavonoids in the product.

[0048] In addition, the linolenic acid-glycerol-based ternary eutectic solvent self-assembles into water-in-oil vesicles during fermentation, which can improve the transdermal absorption of water-soluble active ingredients in the fermentation broth. At the same time, the addition of glycerol significantly improves the stability of water-in-oil vesicles and increases the encapsulation rate of water-soluble active ingredients in oils. Furthermore, as a highly efficient hydrating ingredient, the eutectic solvent prepared from glycerol can further enhance the water-oil balance regulation ability of fermented oils.

[0049] The matsutake endophytic fungus strain XWJ0001 involved in this invention is classified as Umbelopsis sp., with accession number CGMCC No.42463, accession date of December 4, 2025, and deposited at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, No. 1 Beichen West Road, Chaoyang District, Beijing. Detailed Implementation

[0050] To further illustrate the technical means and effects of the present invention, the following describes the technical solution of the present invention in conjunction with preferred embodiments of the present invention. However, the present invention is not limited to the scope of the embodiments.

[0051] The method for preparing the Matsutake endophytic fungal seed liquid described in the following specific embodiments is as follows:

[0052] The endophytic fungus strain XWJ0001 from matsutake mushrooms was added to 10 mL of YPD medium and cultured at 30℃ and 180 rpm for 14 h until mid-log phase to obtain seed culture. The YPD medium formula was: 1% yeast extract, 2% peptone, and 2% glucose, with natural pH.

[0053] Preparation Example 1

[0054] This preparation example provides a eutectic solvent, and the preparation method is as follows:

[0055] The linolenic acid, glycerol, and coconut oil acid were mixed in a molar ratio of 7:3:3 and reacted at 70°C for 2 h to obtain the final product.

[0056] Preparation Example 2

[0057] This preparation example provides a eutectic solvent, and the preparation method is as follows:

[0058] The linolenic acid, glycerol and coconut oil acid were mixed in a molar ratio of 5:1:5 and reacted at 90°C for 1 h to obtain the final product.

[0059] Preparation Example 3

[0060] This preparation example provides a eutectic solvent, and the preparation method is as follows:

[0061] The linolenic acid, glycerol and coconut oil acid were mixed in a molar ratio of 10:5:1 and reacted at 50°C for 4 h to obtain the final product.

[0062] Preparation Example 4

[0063] This preparation example provides a eutectic solvent, and the preparation method is as follows:

[0064] The linolenic acid, glycerol, and lauric acid were mixed in a molar ratio of 7:3:3 and reacted at 70°C for 2 h to obtain the final product.

[0065] Comparative Preparation Example 1

[0066] This preparation example provides a eutectic solvent, and the preparation method is as follows:

[0067] Linoleic acid, glycerol, and coconut oil acid were mixed in a molar ratio of 7:3:3 and reacted at 70°C for 2 h to obtain the final product.

[0068] Comparative Preparation Example 2

[0069] This preparation example provides a eutectic solvent, and the preparation method is as follows:

[0070] The linolenic acid and coconut oil acid were mixed in a molar ratio of 10:3 and reacted at 70°C for 2 h to obtain the product.

[0071] Example 1

[0072] (1) The endophytic fungal seed liquid of matsutake mushroom was fermented in a fermentation medium at an inoculation rate of 10% at a fermentation temperature of 30℃, an aeration rate of 60 L, a stirring speed of 100 rpm, a pressure of 0.2 MPa, and cultured for 18 h to obtain the primary fermentation liquid.

[0073] The fermentation medium, by mass, is formulated as follows: 1% yeast extract, 3% peptone, 3% glycerol, 0.2% potassium dihydrogen phosphate, 0.2% magnesium sulfate, with the remainder being water.

[0074] (2) Mix 45% of the primary fermentation broth with 50% of vegetable oil (marginally meadow flower seed oil, flaxseed oil and camellia oil in a mass ratio of 1:1:1) and 5% of the eutectic solvent prepared in Preparation Example 1, and continue to culture at 30°C for 12 h. After centrifugation, take the upper oil phase to obtain the fermentation oil of matsutake endophytic fungi.

[0075] Example 2

[0076] (1) The endophytic fungal seed liquid of matsutake mushroom was fermented in a fermentation medium at an inoculation rate of 10% at a fermentation temperature of 25℃, an aeration rate of 80 L, a stirring speed of 150 rpm, a pressure of 0.05 MPa, and cultured for 24 h to obtain the primary fermentation liquid.

[0077] The fermentation medium, by mass, consists of: 0.5% yeast extract, 5% peptone, 1% glycerol, 0.3% potassium dihydrogen phosphate, 0.1% magnesium sulfate, with the remainder being water.

[0078] (2) Mix 50% of the primary fermentation broth with 40% of vegetable oil (marginal ratio of meadowfoam seed oil, flaxseed oil and camellia oil of 1:3:1) and 10% of the eutectic solvent prepared in Example 2, and continue to culture at 25°C for 6 h. After centrifugation, take the upper oil phase to obtain the fermentation oil of matsutake endophytic fungi.

[0079] Example 3

[0080] (1) The endophytic fungal seed liquid of matsutake mushroom was fermented in a fermentation medium at an inoculation rate of 10% at a fermentation temperature of 35℃, an aeration rate of 40 L, a stirring speed of 50 rpm, a pressure of 0.3 MPa, and cultured for 6 h to obtain the primary fermentation liquid.

[0081] The fermentation medium, by mass, is formulated as follows: 2% yeast extract, 1% peptone, 5% glycerol, 0.1% potassium dihydrogen phosphate, 0.3% magnesium sulfate, with the remainder being water.

[0082] (2) 42% of the primary fermentation broth was mixed with 54% of vegetable oil (meat ratio of meadowfoam seed oil, flaxseed oil and camellia oil in 3:1:3 mass ratio) and 4% of the eutectic solvent prepared in Preparation Example 3. The mixture was then cultured at 35°C for 24 h. After centrifugation, the upper oil phase was taken to obtain the fermentation oil of matsutake endophytic fungi.

[0083] Example 4

[0084] This embodiment provides a fermented oil of matsutake endophytic fungi, which differs from Example 1 only in that the eutectic solvent obtained in Preparation Example 1 is replaced with the eutectic solvent obtained in Preparation Example 4, while the other steps are consistent with Example 1.

[0085] Example 5

[0086] This embodiment provides a matsutake endophytic fungal fermented oil, which differs from Embodiment 1 only in that the amount of vegetable oil added remains unchanged at 50%, and the vegetable oil is adjusted to a mass ratio of meadowfoam seed oil and flaxseed oil of 1:1. All other steps are consistent with Embodiment 1.

[0087] Example 6

[0088] This embodiment provides a matsutake endophytic fungal fermented oil, which differs from Embodiment 1 only in that the amount of vegetable oil added remains unchanged at 50%, and the vegetable oil is adjusted to a mass ratio of meadowfoam seed oil and camellia oil of 1:1. All other steps are consistent with Embodiment 1.

[0089] Example 7

[0090] This embodiment provides a fermented oil of matsutake endophytic fungi, which differs from Embodiment 1 only in that the amount of vegetable oil added remains unchanged at 50%, and the vegetable oil is adjusted to a mass ratio of 1:1 of flaxseed oil and camellia oil. All other steps are consistent with Embodiment 1.

[0091] Comparative Example 1

[0092] This comparative example provides a fermented oil of matsutake endophytic fungi, which differs from Example 1 only in that the eutectic solvent prepared in Preparation Example 1 is replaced with the eutectic solvent prepared in Comparative Preparation Example 1, while the other steps are consistent with Example 1.

[0093] Comparative Example 2

[0094] This comparative example provides a fermented oil of matsutake endophytic fungi, which differs from Example 1 only in that the eutectic solvent prepared in Preparation Example 1 is replaced with the eutectic solvent prepared in Comparative Preparation Example 2, while the other steps are consistent with Example 1.

[0095] Comparative Example 3

[0096] This comparative example provides a matsutake endophytic fungal fermentation oil, which differs from Example 1 only in that a low eutectic solvent is not added in step (2), that is, 45% of the primary fermentation liquid is mixed with 55% of vegetable oil for fermentation, and the remaining steps are consistent with Example 1.

[0097] Comparative Example 4

[0098] This comparative example provides a fermented oil of matsutake endophytic fungi, which differs from Example 1 only in that the vegetable oil is replaced with synthetic GTCC (caprylic / capric triglyceride) in step (2), that is, 45% primary fermentation broth, 50% GTCC and 5% eutectic solvent prepared in Example 1 are mixed and fermented. The remaining steps are consistent with Example 1.

[0099] Comparative Example 5

[0100] This comparative example provides a matsutake mushroom fermentation liquid, the preparation method of which is as follows:

[0101] Seed culture of the endophytic fungus strain XWJ0001 of matsutake mushroom was inoculated into 10 mL of YPD medium at a 10% inoculation rate and cultured at 30℃ for 5 days. The culture was then centrifuged and filtered to obtain the final product.

[0102] Comparative Example 6

[0103] This comparative example provides a direct-extract oil from matsutake mushrooms, prepared by the following method:

[0104] This comparative example provides a direct-extract oil from matsutake mushrooms, prepared by the following method:

[0105] Wipe fresh matsutake mushrooms clean with a damp cloth, slice them thinly, and soak them in twice their weight of vegetable oil (meadowfoam seed oil, flaxseed oil, and camellia oil in a 1:1:1 ratio). Simmer over low heat for 60 minutes, then filter and collect the filtrate.

[0106] Test Example 1

[0107] Total flavonoid content test:

[0108] (1) Test method:

[0109] Rutin was used as a reference, and aluminum nitrate was used as the colorimetric reagent for the flavonoid colorimetric determination. The absorbance showed a linear relationship with the concentration of rutin, and the total flavonoid content was determined by spectrophotometry. A series of rutin standard stock solutions of different concentrations were prepared (freshly prepared for use). 250 mg of the products from Examples 1-7 and Comparative Examples 1-6 were accurately weighed and added to 5 mL of 80% ethanol. The mixture was sonicated for 30 min, centrifuged at 10000 r / min for 10 min, and the supernatant was collected. Samples were added and measured according to the information shown in Table 1.

[0110] Table 1

[0111]

[0112] (2) Test results:

[0113] The test results of total flavonoid content in the products obtained from each embodiment and comparative example are shown in Table 2.

[0114] Table 2

[0115]

[0116] As can be seen from the data in Table 2, the flavonoid content in the fermented oil involved in this invention is significantly higher than that of matsutake mushroom direct extraction oil obtained by traditional extraction technology.

[0117] Comparison of data from Examples 1 and 4, and Comparative Examples 1-3, shows that by adding a eutectic solvent, the metabolism of endophytic fungi in matsutake mushrooms can be regulated, and the content of flavonoids can be increased. Furthermore, the eutectic solvent prepared from linolenic acid, glycerol, and coconut oil acid can better regulate the metabolism of endophytic fungi in matsutake mushrooms, and improve the targeted enrichment and synthesis of flavonoids.

[0118] Comparison of data from Examples 1, 5-7, and Comparative Example 4 shows that the addition of vegetable oil can further regulate the metabolism of matsutake endophytic fungi and improve the directional accumulation and synthesis of flavonoids. In addition, the combination of meadowfoam seed oil, linseed oil, and camellia oil in the preparation of matsutake endophytic fungal fermentation oil has a significant synergistic effect in increasing the flavonoid content in the product.

[0119] Application Example 1

[0120] This application example provides an essential oil comprising, by weight percentage, 40% squalane, 25% caprylate triglyceride, 15% isononyl isononanoate, 5% matsutake endophytic fungal fermentation oil provided in Example 1, and the balance being olive oil (up to 100%).

[0121] Application Example 2-7

[0122] Application Examples 2-7 each provide an essential oil, which differs from Application Example 1 only in that the matsutake endophytic fungal fermentation oil provided in Example 1 is replaced by an equal mass of matsutake endophytic fungal fermentation oil provided in Examples 2-7, while the rest of the formula remains the same as in Application Example 1.

[0123] Comparative Application Examples 1-6

[0124] Comparative Application Examples 1-6 each provide an essential oil, the only difference from Application Example 1 is that the matsutake endophytic fungal fermented oil provided in Example 1 is replaced by equal masses of matsutake endophytic fungal fermented oil / matsutake fermented liquid / matsutake direct extract oil provided in Comparative Examples 1-6 respectively, and the rest of the formula is the same as Application Example 1.

[0125] Test Example 2

[0126] Moisturizing performance test:

[0127] (1) Test method:

[0128] Seventy-eight participants were randomly divided into 13 groups of six, each using the same essential oil as the control group. Participants washed their hands and forearms, sat quietly for 30 minutes in a controlled humidity environment, and a 3×3cm sample was taken from each forearm. 2 A square experimental area was used, with the left arm as the test area for the sample and the symmetrical area of ​​the right arm as the blank control area. The moisture content (test value) and oil content (blank value) of the test area and the blank control area were detected by a Corneometer CM825 and a Sebumeter SM815, respectively. The test was repeated 3 times and the average value was recorded.

[0129] The formula for calculating skin hydration rate is: Hydration rate = (Test value - Blank value) / Blank value × 100%.

[0130] The formula for calculating the rate of decrease in skin oil content is: Rate of decrease in skin oil content = (test value - blank value) / blank value × 100% (take positive value).

[0131] (2) Test results:

[0132] (2.1) The average skin hydration rate of each sample after 2 h of use is shown in Table 3.

[0133] Table 3

[0134]

[0135] (2.2) The average decrease rate of skin oil content of each sample after 2 h of use is shown in Table 4.

[0136] Table 4

[0137]

[0138] As shown in Tables 3 and 4, the matsutake endophytic fungal fermented oil of this invention has excellent oil-controlling and moisturizing effects. Furthermore, a comparison of the data from Example 1 and Examples 4-7 shows that the selection of the eutectic solvent and the formulation of the vegetable oil also affect the moisturizing and oil-controlling capabilities of the matsutake endophytic fungal fermented oil to a certain extent.

[0139] The applicant declares that the technical solution of this invention is illustrated by the above embodiments, but this invention is not limited to the above embodiments, that is, it does not mean that this invention must rely on the above embodiments to be implemented. Those skilled in the art should understand that any improvements to this invention, equivalent substitutions of raw materials for the products of this invention, addition of auxiliary components, selection of specific methods, etc., all fall within the protection scope and disclosure scope of this invention.

[0140] The preferred embodiments of the present invention have been described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the scope of the technical concept of the present invention, various simple modifications can be made to the technical solution of the present invention, and these simple modifications all fall within the protection scope of the present invention.

[0141] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not describe the various possible combinations separately.

Claims

1. A method for preparing a Tricholoma matsutake endophytic fungus fermentation oil having oil control and water replenishment effects, characterized by, The preparation method includes: (1) The seed liquid of matsutake endophytic fungi was inoculated into a fermentation medium for fermentation culture to obtain a primary fermentation broth; (2) The primary fermentation broth is mixed with vegetable oil and linolenic acid-glycerol ternary eutectic solvent, wherein the mass ratio of the primary fermentation broth, vegetable oil and linolenic acid-glycerol ternary eutectic solvent is (30-60):(40-60):(1-10), and fermentation is continued. The upper oil phase is collected by centrifugation to obtain matsutake endophytic fungal fermentation oil. The linolenic acid-glycerol-based ternary eutectic solvent is obtained by reacting linolenic acid, glycerol and coconut oil acid in a molar ratio of (5-10):(1-5):(1-5) at 50-90℃. The vegetable oil is a combination of meadowfoam seed oil, flaxseed oil and camellia oil in a mass ratio of (1-3):(1-3):(1-3); The matsutake endophytic fungus mentioned in step (1) is the matsutake endophytic fungus strain XWJ0001, which is classified as Umbelopsissp. and has the preservation number CGMCC No.42463.

2. The production method according to claim 1, characterized by, The fermentation medium in step (1) includes: yeast powder, peptone, glycerol, potassium dihydrogen phosphate, magnesium sulfate and water.

3. The preparation method according to claim 1, characterized in that, In step (1), the inoculation volume of the matsutake endophytic fungal seed solution is 1-10% of the fermentation medium volume; The fermentation culture temperature in step (1) is 25-35℃, and the fermentation culture time is 6-24 h; The fermentation temperature in step (2) is 25-35℃, and the fermentation time is 6-24 h.

4. A fermented oil of matsutake endophytic fungi prepared by any one of claims 1-3.

5. The application of the matsutake endophytic fungal fermented oil according to claim 4 in the preparation of cosmetics.

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