一种基于电荷定向交联的酶固定方法
The enzyme immobilization method, which utilizes charge-directed crosslinking and multivalent metal ion enhancement, solves the problems of conformational distortion of enzyme active sites and low immobilization amount in enzyme immobilization, achieving efficient enzyme catalysis and enhanced stability, and optimizing enzyme spatial orientation and substrate mass transfer pathways.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- SUZHOU ZHEYUAN AUTOMATION ENG TECH CO LTD
- Filing Date
- 2026-01-05
- Publication Date
- 2026-07-17
AI Technical Summary
In existing enzyme immobilization technologies, covalent cross-linking leads to conformational distortion of the enzyme's active site, physical adsorption results in low immobilization capacity and easy desorption of enzyme molecules, and traditional encapsulation methods face high substrate diffusion resistance, making it difficult to maintain the enzyme's native conformation and optimize substrate mass transfer pathways.
A charge-directed crosslinking method was adopted to modify the enzyme surface with anionic groups and form an electrostatic crosslinked gel network with a carrier carrying protonated amino groups under acidic or alkaline conditions. Multivalent metal ions were used to enhance the stability of the network, avoid conformational distortion caused by covalent bonds, and optimize the distribution of enzyme molecules in the gel.
It achieves high activity retention, controllable immobilization and enhanced operational stability, improves enzyme catalytic efficiency and reusability, optimizes enzyme spatial orientation and substrate mass transfer pathway, and reduces enzyme activity decay and enzyme leakage problems.
Smart Images
Figure CN121450630B_ABST