A sampling device for groundwater microbial detection

Abstract

The application discloses a sampling device for underground water microorganism detection and belongs to the technical field of underground water detection equipment. The sampling rotating mechanism comprises a fixed column, a rotating lifting sleeve is sleeved on the fixed column, a guide block is slidably connected in the rotating lifting sleeve, and a return spring is fixedly connected between the guide block and the rotating lifting sleeve. A sliding groove is dug on the fixed column, the sliding groove comprises a plurality of first-end and last-end communicating inclined lifting grooves and straight moving grooves. A sliding sleeve is rotatably connected to the upper end of the rotating lifting sleeve through a fixing sleeve, a limiting block is slidably connected to the inner side wall of the sliding sleeve and fixedly connected to the side wall of the fixed column, an L-shaped pressing rod is fixedly connected to the side wall of the sliding sleeve, and a sampling mechanism is rotatably connected to the fixed column. The sampling device can improve the remote management capability of the plurality of sampling mechanisms.

Description

Technical Field

[0001] This invention relates to the field of groundwater testing equipment technology, specifically a sampling device for detecting microorganisms in groundwater. Background Technology

[0002] Groundwater microbial testing refers to the comprehensive analysis of the types, quantities, activities, and community structure of microorganisms in groundwater samples using a series of professional microbiological testing techniques and methods. By collecting groundwater samples and, under laboratory conditions, using methods such as culture and molecular biology techniques (e.g., PCR, high-throughput sequencing), the presence of pathogenic microorganisms (such as Escherichia coli and Salmonella, which may harm human health) and various indicator microorganisms (microorganisms used to reflect the degree of groundwater pollution or ecological status) is detected. This assessment evaluates the microbial quality and safety of the groundwater, determines whether it meets relevant water quality standards, and provides a scientific basis for groundwater development and utilization, pollution prevention and control, and ecological environmental protection.

[0003] A Chinese patent discloses a groundwater sampling device for different depths used in groundwater surveys (authorization announcement number CN118624295B). The patent includes a ring frame, a mounting base, a sampling tube, a limiting component, an adjustment component, and a pulling frame.

[0004] Most existing groundwater sampling devices are single-use and cannot be used for multiple samplings at different depths. Therefore, multiple samplings are required to sample groundwater at different depths. To prevent the sampling tube from contaminating the groundwater collected next time, the sampling device also needs to be cleaned, which is inconvenient and inefficient. The patent mentioned above solves the problem of needing to repeatedly sample groundwater at different depths. It requires a waterproof push rod to provide upward driving force, but the length of the waterproof push rod determines the depth of groundwater that can be sampled. Therefore, a longer waterproof push rod is not suitable for sampling deeper groundwater. In addition, due to the large temperature difference between the ground and the surface, if the groundwater is stored for a long time after sampling, the metabolism and growth rate of microorganisms in the water sample will change, thus affecting the detection results of microorganisms. Therefore, we propose a sampling device for groundwater microbial detection. Summary of the Invention

[0005] The purpose of this invention is to provide a sampling device for detecting microorganisms in groundwater, so as to solve the problems mentioned in the background art.

[0006] To achieve the above objectives, the present invention provides the following technical solution: A sampling device for detecting microorganisms in groundwater includes a sampling rotation mechanism, and further includes: A fixed column is fitted with a lifting sleeve. A guide block is slidably connected inside the lifting sleeve, and a return spring is fixedly connected between the guide block and the lifting sleeve. A sliding groove is chiseled on the fixed column, and the sliding groove includes multiple inclined lifting grooves and straight moving grooves that are connected end to end. A sliding sleeve is rotatably connected to the upper end of the lifting sleeve through the fixed sleeve. A limiting block that is slidably connected to the side wall of the fixed column is fixedly connected to the inner side wall of the sliding sleeve, and an L-shaped pressure rod is fixedly connected to the side wall of the sliding sleeve. The sampling mechanism, which is rotatably connected to the fixed column, includes a rotating sleeve and a sampling cylinder fixedly connected to the rotating sleeve by multiple fixed bent rods. The sampling cylinder draws water from the source by negative pressure suction. A piston is slidably connected inside the sampling cylinder, and the piston is fixedly connected to the upper inner wall of the sampling cylinder by a connecting spring. A pull rod that penetrates the upper end of the sampling cylinder is fixedly connected to the upper side wall of the piston, and an insertion hole is drilled on the pull rod. A limiting mechanism, disposed at the upper end of the sampling cylinder, includes a limiting cylinder fixedly connected to the upper end of the rotating sleeve, with a pull rod passing through the limiting cylinder. Two fixed cylinders are fixedly connected to the side wall of the limiting cylinder, each fixed cylinder having a limit block fixedly connected to it, and each fixed cylinder having a rod inserted into it that is slidably connected to the corresponding limit block. Two rotating sleeves are rotatably connected to the fixed cylinder, with a pressing plate fixedly connected between the two rotating sleeves, and a torsion spring fixedly connected between each rotating sleeve and the limiting cylinder. The ends of the two rods away from the limiting cylinder are fixedly connected to a movable cylinder with a groove carved on its side wall, and a slider fixedly connected to the inner side wall of the rotating sleeve is slidably connected inside the groove. The rods are matched with the insertion holes. The lower end of the sampling tube is equipped with a sealing mechanism, which is used to intercept and filter microorganisms and impurities during the sampling process. A prompt block is fixedly connected to the upper end of the pull rod. The lower end of the sampling tube is equipped with a filtering mechanism, and a winding depth measuring mechanism is installed on the fixed column.

[0007] As a further embodiment of the present invention, the sealing mechanism includes a sealing cover, a drain check valve is inserted into the sealing cover, the sealing cover is fixedly connected to the pull rod through two connecting rods, and a filter screen sleeve fitted on the sampling cylinder is fixedly connected to the upper end of the sealing cover.

[0008] As a further embodiment of the present invention, a semiconductor cooling chip is fixedly connected to the inner wall of the sealing cover.

[0009] As a further embodiment of the present invention, the colors of multiple indicator blocks change from dark to light in a counterclockwise direction.

[0010] As a further embodiment of the present invention, the filtration mechanism includes an annular block fixedly connected to the lower end of the sampling cylinder, an ultrafiltration screen fixedly disposed on the annular block, and an inlet check valve disposed at the center of the ultrafiltration screen.

[0011] As a further embodiment of the present invention, the winding depth measuring mechanism includes a winding shell, a winding drum is provided inside the winding shell, and the winding drum is rotatably connected to the winding shell through two cylinders. A winding tube is wound on the winding drum, and a handle is fixedly connected to the side wall of the winding drum.

[0012] As a further embodiment of the present invention, the winding tube is provided with scale lines.

[0013] As a further embodiment of the present invention, an air cylinder is fixedly connected to the side wall of the winding shell, an air plug is slidably connected inside the air cylinder, two limiting rods that penetrate the air cylinder are fixedly connected to the air plug, and a threaded rod that is threadedly connected to the air cylinder is rotatably connected to the air plug.

[0014] As a further embodiment of the present invention, the lower end of the lifting sleeve is fixedly connected to a base plate through multiple transmission rods passing through the rotating sleeve, and all the transmission rods are arranged to pass through the rotating sleeve.

[0015] As a further embodiment of the present invention, a threaded rod that is rotatably connected to the bottom plate is fixedly connected to the lower end of the fixed column, and the winding tube passes through the fixed column and communicates with the threaded rod.

[0016] Compared with the prior art, the beneficial effects of the present invention are: When in use, this invention allows for the collection of groundwater by using a combination of sampling, limiting, and sealing mechanisms. After the sampling rotation mechanism reaches the specified underground depth, the sampling mechanisms can be opened one by one by a winding depth sounding mechanism. This device can improve the remote management capability of multiple sampling mechanisms. Furthermore, through the combination of sealing and filtration mechanisms, most microorganisms and small impurities can be intercepted at the ultrafiltration screen during the groundwater sampling process. The temperature at the groundwater sampling depth is maintained constant by using a semiconductor cooling chip. Attached Figure Description

[0017] Figure 1 A three-dimensional view of a sampling device for detecting microorganisms in groundwater; Figure 2 This is a schematic diagram of the sampling mechanism in a sampling device for detecting microorganisms in groundwater. Figure 3 This is a schematic diagram of the sampling rotation mechanism and sampling mechanism part in a sampling device for detecting microorganisms in groundwater; Figure 4 This is a schematic diagram of the limiting mechanism in a sampling device for detecting microorganisms in groundwater; Figure 5 This is a schematic diagram of the chute structure in a sampling device for detecting microorganisms in groundwater; Figure 6 This is a schematic diagram of the internal structure of a sampling tube in a sampling device for detecting microorganisms in groundwater; Figure 7 An exploded view of the limiting mechanism in a sampling device for detecting microorganisms in groundwater; Figure 8 This is a schematic diagram of the sealing mechanism in a sampling device for detecting microorganisms in groundwater; Figure 9 This is a schematic diagram of the structure of the winding depth sounding mechanism in a sampling device for detecting groundwater microorganisms. Figure 10 This is a diagram showing the state of a sampling device used for groundwater microbial detection when the sampling mechanism extracts groundwater.

[0018] In the picture: 1. Sampling rotation mechanism; 101. Fixed column; 102. Lifting sleeve; 103. Slide groove; 1031. Inclined lifting groove; 1032. Straight movement groove; 104. Guide block; 105. Return spring; 2. Sampling mechanism; 201. Rotating sleeve; 202. Fixed bent rod; 203. Sampling cylinder; 204. Piston; 205. Pull rod; 206. Connecting spring; 3. Restriction mechanism; 301. Restriction cylinder; 302. Fixed cylinder; 303. Insert rod; 304. Limiting block; 305. Rotating sleeve; 306. Moving cylinder; 307. Inclined groove; 308. Sliding block; 309. Pressing plate; 310. Torsion spring; 311. Insertion hole; 312. Fixed sleeve; 313. Sliding sleeve; 314. Restriction block; 315. L-shaped pressure rod; 4. Sealing mechanism; 401. Sealing cover; 402. Drain check valve; 403. Connecting rod; 404. Filter screen cylinder; 405. Semiconductor refrigeration chip; 5. Prompt block; 6. Filtration mechanism; 601. Annular block; 602. Ultrafiltration screen; 603. Inlet check valve; 7. Rewinding depth measuring mechanism; 701. Rewinding shell; 702. Rewinding drum; 703. Rewinding tube; 704. Cylinder; 705. Handle; 706. Air pump; 707. Air plug; 708. Limiting rod; 709. Threaded rod; 710. Transmission rod; 711. Base plate. Detailed Implementation

[0019] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0020] Example 1: Please refer to Figures 1 to 10In this embodiment of the invention, a sampling device for groundwater microbial detection includes a sampling rotation mechanism 1. This device must meet the requirements of HJ164-2020 "Technical Specification for Groundwater Environmental Monitoring," which stipulates that the sampling equipment should not alter the chemical composition of the groundwater and that the water sample to be tested should be rinsed 2-3 times before sampling. The sampling rotation mechanism 1 includes a fixed column 101, which is a plastic cylinder formed by injection molding. The fixed column 101 has a cavity reserved inside for placing a take-up tube 703. A lifting sleeve 102 is fitted onto the fixed column 101. A groove 103, formed by the mold during the injection molding process, is chiseled on the fixed column 101. The groove 103 includes multiple grooves of varying depths from bottom to top. The inclined riser 1031 has a depth that decreases from deep to shallow, and the straight mover 1032 has a depth that decreases from shallow to deep from bottom to top. The inclined riser 1031 and the straight mover 1032 are connected end to end, so that the guide block 104 can switch back and forth between the inclined riser 1031 and the straight mover 1032 during the movement. The angle between the two ends of the inclined riser 1031 and the center of the circle is 90 degrees. The guide block 104 is slidably connected to the inner side wall of the lifting sleeve 102 through the excavated installation groove. A return spring 105 for extending and retracting the guide block 104 is fixedly connected between the guide block 104 and the lifting sleeve 102. In the initial state, the return spring 105 is located at the upper part of the straight mover 1032. A sampling mechanism 2 for extracting groundwater is rotatably connected to the fixed column 101. refer to Figure 2 , Figure 3 , Figure 6 and Figure 7The sampling mechanism 2 includes a rotating sleeve 201 rotatably connected to a fixed column 101 via a mechanical seal. The rotating sleeve 201 is made of stainless steel. The mechanical seal effectively reduces the entry of impurities from groundwater into the gap between the rotating sleeve 201 and the fixed column 101, improving the structural durability. Multiple fixed bent rods 202 are welded to the sidewalls of the rotating sleeve 201 and fixedly connected to the sampling cylinder 203. The sampling cylinder 203 must meet the requirements of HJ / T164-2004 "Technical Specification for Groundwater Environmental Monitoring," which stipulates that microbial sampling containers should be pre-sterilized and that external microbial contamination should be avoided during the sampling process. The sampling cylinder 203 is made of two layers of 304 stainless steel with a vacuum interlayer in between, effectively isolating external temperatures and ensuring that the extracted groundwater remains at a constant temperature for a short time. An exhaust vent is drilled at the top of the sampling cylinder 203. When the piston 204 moves, it allows air to enter and exit the upper part of the sampling cylinder 203. The piston 204 is slidably connected inside the sampling cylinder 203. The piston 204 is made of hollow stainless steel, which reduces weight while also having a certain degree of hardness to prevent deformation during use. Two layers of rubber rings are fixedly connected to the side wall of the piston 204 to improve the sealing between it and the inner wall of the sampling cylinder 203. A pull rod 205 is fixedly connected to the upper side wall of the piston 204 by welding, and the pull rod 205 passes through the upper end of the sampling cylinder 203. A connecting spring 206 is fixedly connected between the piston 204 and the upper inner wall of the sampling cylinder 203 to pull the piston 204 upward. Before the groundwater is extracted, the connecting spring 206 is in a stretched state. A limiting mechanism 3 is provided at the upper end of the sampling cylinder 203 to limit the pull rod 205. refer to Figure 3 , Figure 4 and Figure 7As shown, the limiting mechanism 3 includes a limiting cylinder 301 welded and fixed to the upper end of the rotating sleeve 201, and a pull rod 205 passing through the limiting cylinder 301. Two fixed cylinders 302 are fixedly connected and communicated on the side wall of the limiting cylinder 301. Inserted rods 303 are inserted into each of the two fixed cylinders 302. Limiting blocks 304, which are fixedly connected to the side walls of the two inserted rods 303, are slidably connected to the side walls of the two inserted rods 303, so that the limiting blocks 304 restrict the inserted rods 303 and prevent the inserted rods 303 from rotating. A rotating sleeve 305 is rotatably connected to the fixed cylinder 302. A movable cylinder 306 is fixedly connected to the end of each of the two inserted rods 303 away from the limiting cylinder 301. A slanted groove 307 is carved into the side wall of sleeve 06. The two ends of the slanted groove 307 form a 90-degree angle with the center of the moving cylinder 306, meaning the maximum rotation angle between the rotating sleeve 305 and the pressing plate 309 is 90 degrees. A slider 308, which is fixedly connected to the inner side wall of the rotating sleeve 305, is slidably connected inside the slanted groove 307. When the pressing plate 309 rotates downward, the slider 308 can drive the moving cylinder 306 to move through the slanted groove 307. The moving cylinder 306 drives the insert rod 303 to be pulled out from the insertion hole 311 of the pull rod 205. A pressing plate 309 is fixedly connected between the two rotating sleeves 305. The end of the pressing plate 309 away from the rotating sleeve 305 is tilted upward to reduce the pressure. The reset force of the pressure plate 309 is achieved by a torsion spring 310 fixedly connected between the rotating sleeve 305 and the limiting cylinder 301. An annular plate is provided at one end of the rotating sleeve 305 near the limiting cylinder 301. The limiting cylinder 301, located between the annular plate and the limiting cylinder 301, can reset the pressure plate 309. Two sets of insertion holes 311 are drilled on the pull rod 205 to match the insertion rod 303. When the pull rod 205 is released from its limit and moves upward, the pressure plate 309 resets via the torsion spring 310, allowing the moving cylinder 306 to push the insertion rod 303 into the insertion hole 311 again, thus completing a secondary locking of the pull rod 205. The sleeve is then rotated upwards. The upper end of the sleeve 102 is rotatably connected to the sliding sleeve 313 via the fixed sleeve 312. The upper end of the lifting sleeve 102 is welded and fixed to the fixed sleeve 312. The inner side wall of the sliding sleeve 313 is fixedly connected to the limiting block 314 which is slidably connected to the side wall of the fixed column 101. The fixed column 101 is chiseled with a groove that matches the limiting block 314, so that the sliding sleeve 313 can only move up and down in the fixed column 101. The side wall of the sliding sleeve 313 is fixedly connected to the L-shaped pressure rod 315. The lower end of the L-shaped pressure rod 315 corresponds to the pressing plate 309. The lower end of the L-shaped pressure rod 315 is provided with a rotatable rubber cylinder to reduce the friction between it and the pressing plate 309. When an upward force is applied to the lifting sleeve 102, the lifting sleeve 102 moves from the lower part to the upper part of the inclined groove 1031 via the guide block 104. Since the depth of the inclined groove 1031 decreases from deep to shallow from bottom to top, the return spring 105 is in a compressed state after it moves to the upper part of the inclined groove 1031. As the lifting sleeve 102 continues to move upward, the guide block 104 slides into the upper part of the straight groove 1032. At the same time, the lifting sleeve 102 spirals upward counterclockwise relative to the fixed column 101, so that the lifting sleeve 102 can drive the multiple sampling mechanisms 2 to rotate 90 degrees through the transmission rod 710 and the rotating sleeve 201. When a downward force is applied to the lifting sleeve 102, the lifting sleeve 102 moves from the upper part to the lower part of the straight groove 1032 via the guide block 104. Since the depth of the straight groove 1032 increases from bottom to top, the return spring 105 is also in a compressed state after moving to the lower part of the straight groove 1032. As the lifting sleeve 102 continues to move downward, the guide block 104 slides into the lower part of the inclined groove 1031. At the same time, the lifting sleeve 102... 02 The sliding sleeve 313 is pulled down by the fixed sleeve 312. The sliding sleeve 313 presses the pressing plate 309 through the L-shaped pressure rod 315. When the pressing plate 309 flips, it drives the rotating sleeve 305 and the slider 308 to rotate. When the slider 308 rotates, it guides the moving cylinder 306 to move through the inclined groove 307, so that the moving cylinder 306 pulls out the insertion rod 303. At this time, after the pull rod 205 is released from the limit, the piston 204 moves up under the pulling force of the connecting spring 206 to extract groundwater.

[0021] refer to Figure 6 and Figure 8 As shown, a sealing mechanism 4 for sealing the lower part of the sampling cylinder 203 is provided at the lower end of the sampling cylinder 203, a prompting block 5 for prompting the staff to take samples is fixedly connected to the upper end of the pull rod 205, a filtering mechanism 6 mainly for intercepting microorganisms is provided at the lower end of the sampling cylinder 203, and a winding depth measuring mechanism 7 for measuring the sampling depth and controlling the sampling of the sampling mechanism 2 is provided on the fixed column 101.

[0022] The sealing mechanism 4 includes a sealing cover 401. A sealing ring is provided between the sealing cover 401 and the lower end of the sampling cylinder 203 to improve the sealing performance between the sealing cover 401 and the sampling cylinder 203. A drain check valve 402 for draining excess groundwater is inserted into the sealing cover 401. When the drain check valve 402 covers the lower end of the sampling cylinder 203, excessive groundwater in the sampling cylinder 203 may prevent the sealing cover 401 from sealing tightly. The drain check valve 402 can drain the excess groundwater in the sampling cylinder 203, improving the sealing effect of the sealing cover 401. The sealing cover 401 is fixedly connected to the pull rod 205 by two connecting rods 403. A filter for filtering larger impurities is fixedly connected to the upper end of the sealing cover 401 and sleeved on the sampling cylinder 203. The filter cylinder 404 can prevent larger particulate impurities from entering the sealing cover 401, which would worsen the seal between the sealing cover 401 and the bottom of the sampling cylinder 203. A semiconductor cooling chip 405 is fixedly connected to the inner wall of the sealing cover 401. By setting a temperature sensor inside the sampling cylinder 203, the temperature at the current sampling depth can be measured when sampling groundwater. After sampling, the sampling cylinder 203 is kept at a constant temperature for the groundwater, and the temperature change inside the sampling cylinder 203 is continuously monitored by the temperature sensor. When the temperature difference inside the sampling cylinder 203 exceeds 2℃, the semiconductor cooling chip 405 and the drive circuit are used for heating or cooling, and the temperature is precisely adjusted by the PID control algorithm to maintain the water temperature within the target value ±2℃ range.

[0023] Multiple indicator blocks 5 change color from dark to light in a counterclockwise direction. By observing the indicator blocks 5 during multiple underground samplings, the sampling order of each sampling mechanism 2 can be determined based on the color intensity and the rotation direction of the multiple sampling mechanisms 2.

[0024] refer to Figure 8 and Figure 10 As shown, the filtration mechanism 6 includes an annular block 601 fixedly connected to the lower end of the sampling cylinder 203. The annular block 601 and the sampling cylinder 203 are designed as a single unit and are fixed inside the sampling cylinder 203 by threads, so that the annular block 601 can be removed for microbial testing and the contact force of the sealing ring can be adjusted. An ultrafiltration screen 602 for intercepting microorganisms is fixedly connected to the annular block 601. The pore size of the ultrafiltration screen 602 is 0.45μm. A one-way valve 603 is installed at the center of the ultrafiltration screen 602. The one-way valve 603 will not open when the ultrafiltration screen 602 is clear. When the ultrafiltration screen 602 is clogged due to too many small impurities, the one-way valve 603 can open, so that more groundwater can be extracted for testing.

[0025] The ultrafiltration screen 602 can be replaced with different mesh sizes as needed to achieve different filtration and interception effects.

[0026] Example 2: Please refer to Figure 9 Based on Embodiment 1, the winding depth measuring mechanism 7 includes an integrally die-cast winding shell 701 made of plastic, which effectively reduces weight and facilitates carrying. A winding drum 702 is installed inside the winding shell 701, and the winding drum 702 is rotatably connected to the side wall of the winding shell 701 via two cylinders 704. A winding tube 703 is wound around the winding drum 702. The winding tube 703 is made of PVC-U rigid polyvinyl chloride water pipe, which has high hardness, good pressure resistance, is not easily flattened, and has low cost. A handle 705 is fixedly connected to the side wall of the winding drum 702 to drive the winding drum 702 to unwind the winding tube 703. The winding tube 703 is provided with scale lines for observing the sampling depth. An air cylinder 706 is fixedly connected to the side wall of the winding shell 701. The air cylinder 706, the winding tube 703, and the threaded rod 709 are connected... The cylinder 706 is filled with hydraulic oil and has an air plug 707 slidably connected inside. Two limiting rods 708 are fixedly connected to the air plug 707, which are set through the air cylinder 706. The limiting rods 708 prevent the air plug 707 from rotating. A threaded rod 709 is rotatably connected to the air plug 707 and threadedly connected to the air cylinder 706. The lower end of the lifting sleeve 102 is fixedly connected to the base plate 711 through multiple transmission rods 710, and all multiple transmission rods 710 are set through the rotating sleeve 201. The lower end of the fixed column 101 is fixedly connected to the threaded rod 709, and the threaded rod 709 is rotatably connected to the base plate 711. When the threaded rod 709 extends, it can drive the base plate 711 to move downward, so that the base plate 711 can apply a force relative to the fixed column 101 to the lifting sleeve 102 through the multiple transmission rods 710. The winding tube 703 passes through the fixed column 101 and communicates with the threaded rod 709.

[0027] The working principle of this invention is: In this invention, during sampling, the operator first inserts the sampling rotating mechanism 1 into the sampling well, slowly rotates the handle 705 to unwind the take-up tube 703, and allows the sampling rotating mechanism 1 to gradually sink into the water to the sampling depth. Then, the threaded rod 709 is rotated to move the air plug 707. At this time, the hydraulic oil in the air cylinder 706 is transported to the threaded rod 709 through the take-up tube 703. The threaded rod 709 extends and drives the base plate 711 to move down. The base plate 711 then applies a force to the lifting sleeve 102 relative to the fixed column 101 through multiple transmission rods 710. At this time, the lifting sleeve 102 moves from the upper part to the lower part of the straight sliding groove 1032 via the guide block 104. Because the straight sliding groove 1032 is shallow at the bottom and deep at the top, the return spring 105 is also in a compressed state when it moves to the lower part of the straight sliding groove 1032. As the lifting sleeve 102 continues to move down, the guide block 104 slides into the lower part of the inclined lifting groove 1031. At the same time, the lifting sleeve 102 pulls the sliding sleeve 313 down through the fixed sleeve 312. The sliding sleeve 313 presses the pressing plate 309 through the L-shaped pressure rod 315, causing the pressing plate 309 to flip and drive the rotating sleeve 305 and the slider 308 to rotate. When the slider 308 rotates, it guides the moving cylinder 306 to move through the inclined groove 307, causing the moving cylinder 306 to pull out the insertion rod 303. The pull rod 205 is released from the limit, and the piston 204 moves up under the pulling force of the connecting spring 206 to extract groundwater. In this process, large particles of impurities are first filtered by the filter cylinder 404, while smaller particles and microorganisms are intercepted by the ultrafiltration screen 602. If the ultrafiltration screen 602 is clogged by tiny impurities, the inlet check valve 603 opens under pressure to extract more groundwater for testing. Simultaneously, the temperature sensor inside the sampling cylinder 203 measures the temperature at the current sampling depth and records it with the microcontroller when sampling groundwater. When the piston 204 moves upward, it drives the sealing cover 401 to move upward through the connecting rod 403 to seal the lower port of the sampling cylinder 203. Afterward, the operator reverses the threaded rod 709 to reset the air plug 707. The threaded rod 709 retracts under the transmission of hydraulic oil, pulling the base plate 711. The base plate 711 drives the guide block 104 in the lifting sleeve 102 through multiple transmission rods 710. As the inclined riser 1031 moves from the lower part to the upper part, because the inclined riser 1031 is deeper at the bottom and shallower at the top, the return spring 105 is compressed after moving to the upper part of the inclined riser 1031. Finally, the lifting sleeve 102 continues to move upward, causing the guide block 104 to slide into the upper part of the straight movement groove 1032. At the same time, the lifting sleeve 102 spirals upward counterclockwise relative to the fixed column 101, driving multiple sampling mechanisms 2 to rotate 90 degrees through the transmission rod 710 and the rotating sleeve 201, thereby allowing a sampling mechanism 2 to be replaced for the next sampling. The staff can then continue to rotate handle 705 to move the sampling rotation mechanism 1 down to change the depth and continue sampling. When a second sampling is required, the staff can rotate the threaded rod 709 again and repeat the above steps. After sampling, the sampling tube 203 is kept at a constant temperature for the groundwater and the temperature change inside the sampling tube 203 is continuously monitored by a temperature sensor. When the temperature difference inside the sampling tube 203 exceeds 2℃, the semiconductor cooling chip 405 and the driving circuit are used for heating or cooling, and the temperature is precisely adjusted by a PID control algorithm to maintain the water temperature within the target value ±2℃ range.

[0028] The above are merely preferred embodiments of the present invention, but the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and inventive concept of the present invention, should be covered within the scope of protection of the present invention.

Claims

1. A sampling device for detecting microorganisms in groundwater, comprising a sampling rotation mechanism (1), characterized in that, Also includes: A fixed column (101) is fitted with a lifting sleeve (102). A guide block (104) is slidably connected inside the lifting sleeve (102), and a return spring (105) is fixedly connected between the guide block (104) and the lifting sleeve (102). A sliding groove (103) is chiseled on the fixed column (101). The sliding groove (103) includes multiple end-to-end connected inclined grooves (1031) and straight grooves (1032). A sliding sleeve (313) is rotatably connected to the upper end of the lifting sleeve (102) through a fixed sleeve (312). A limiting block (314) is fixedly connected to the inner side wall of the sliding sleeve (313) and slidably connected to the side wall of the fixed column (101). An L-shaped pressure rod (315) is fixedly connected to the side wall of the sliding sleeve (313). The sampling mechanism (2), which is rotatably connected to the fixed column (101), includes a rotating sleeve (201) and a sampling cylinder (203) fixedly connected to the rotating sleeve (201) through multiple fixed bent rods (202), wherein the sampling cylinder (203) draws water from the source by negative pressure suction. A limiting mechanism (3) is provided at the upper end of the sampling cylinder (203), including a limiting cylinder (301) fixedly connected to the upper end of the rotating sleeve (201), and a pull rod (205) passing through the limiting cylinder (301). Two fixed cylinders (302) are fixedly connected to the side wall of the limiting cylinder (301), and each fixed cylinder (302) is fixedly connected to a limit block (304), and each fixed cylinder (302) is inserted with a rod (303) that is slidably connected to the corresponding limit block (304). Two rotating sleeves (305) are rotatably connected to the upper part of the device. A pressing plate (309) is fixedly connected between the two rotating sleeves (305), and a torsion spring (310) is fixedly connected between each rotating sleeve (305) and the limiting cylinder (301). The ends of the two insert rods (303) away from the limiting cylinder (301) are fixedly connected to a movable cylinder (306) with a groove (307) cut on the side wall. A slider (308) that is fixedly connected to the inner side wall of the rotating sleeve (305) is slidably connected inside the groove (307). The sampling tube (203) is provided with a sealing mechanism (4) at its lower end, which is used to intercept and filter microorganisms and impurities during the sampling process; The upper end of the pull rod (205) is fixedly connected to a prompting block (5), the lower end of the sampling tube (203) is provided with a filtering mechanism (6), and the fixed column (101) is provided with a winding depth measuring mechanism (7). The sealing mechanism (4) includes a sealing cover (401), a drain check valve (402) is inserted into the sealing cover (401), the sealing cover (401) is fixedly connected to the pull rod (205) through two connecting rods (403), a filter screen cylinder (404) sleeved on the sampling cylinder (203) is fixedly connected to the upper end of the sealing cover (401), and a semiconductor cooling chip (405) is fixedly connected to the inner wall of the sealing cover (401). A piston (204) is slidably connected inside the sampling cylinder (203), and the piston (204) is fixedly connected to the upper inner wall of the sampling cylinder (203) by a connecting spring (206); a pull rod (205) is fixedly connected to the upper side wall of the piston (204) and passes through the upper end of the sampling cylinder (203); an insertion hole (311) is drilled on the pull rod (205), and the insertion rod (303) matches the insertion hole (311); The filtration mechanism (6) includes an annular block (601) fixedly connected to the lower end of the sampling cylinder (203), an ultrafiltration screen (602) fixedly disposed on the annular block (601), and an inlet check valve (603) disposed at the center of the ultrafiltration screen (602). The winding depth measuring mechanism (7) includes a winding shell (701), inside which a winding drum (702) is provided, and the winding drum (702) is rotatably connected to the winding shell (701) through two cylinders (704). A winding tube (703) is wound on the winding drum (702), and a handle (705) is fixedly connected to the side wall of the winding drum (702). An air cylinder (706) is fixedly connected to the side wall of the winding shell (701), and an air plug (707) is slidably connected inside the air cylinder (706). The upper part has two limiting rods (708) that pass through the air cylinder (706) and are fixedly connected to it. The air plug (707) has a threaded rod (709) that is threaded to the air cylinder (706). The lower end of the lifting sleeve (102) passes through the rotating sleeve (201) through multiple transmission rods (710) and is fixedly connected to the base plate (711). The lower end of the fixed column (101) has a threaded rod (709) that is rotatably connected to the base plate (711). The winding tube (703) passes through the fixed column (101) and communicates with the threaded rod (709).

2. The sampling device for groundwater microbial detection according to claim 1, characterized in that, The colors of the multiple prompt blocks (5) change from dark to light in a counterclockwise direction.

3. A sampling device for groundwater microbial detection according to claim 2, characterized in that, The winding tube (703) is provided with scale lines.

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