一种检测马立克氏病病毒MEQ基因的引物对、试剂盒及其检测方法与应用
By providing specific primer pairs and kits, combined with MIRA amplification and CRISPR/Cas12a reaction, the problem of high cost and long time time of existing Marek's disease virus detection equipment is solved, realizing rapid, low-cost, and sensitive virus detection, suitable for field applications.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- SOUTH CHINA AGRICULTURAL UNIVERSITY
- Filing Date
- 2026-01-07
- Publication Date
- 2026-07-17
AI Technical Summary
Existing methods for detecting Marek's disease virus require bulky or expensive instruments and equipment, take a long time to complete, and have high requirements for testing personnel. They are only suitable for laboratory-assisted diagnosis and cannot be widely applied.
This invention provides a primer pair and kit for detecting the MEQ gene of Marek's disease virus, which, combined with MIRA amplification and a CRISPR/Cas12a reaction system, enables rapid and specific detection, suitable for mobile on-site testing.
It enables rapid and accurate detection of Marek's disease virus, reduces the requirements for instruments and equipment and personnel skills, is suitable for field applications, is low in cost and highly sensitive, and has a low false positive rate.
Smart Images
Figure CN121472489B_ABST