A detection device for detecting escherichia coli

Abstract

The application discloses a detection device for detecting escherichia coli, which comprises a detector shell and a sampling tube, the sampling tube is in an upper-lower split structure, and the upper part and the lower part of the sampling tube are mutually inserted, one side of the detector shell is provided with a fixing frame, and a fixed rod and a movable rod are arranged in the fixing frame. In the implementation of the device, the upper part and the lower part of the sampling tube can be separated through the driving of the connecting block, and when the upper part of the sampling tube moves to a predetermined position, the upper part of the sampling tube no longer moves upward along with the continuous upward movement of the connecting block; at this time, the sample is placed on the sample rack and pushed into the fixing frame, the cotton swab in the sampling tube can fully contact the surface of the sample to realize the sampling purpose, and then the sampling tube is placed into the detector, so that the escherichia coli in the sample can be detected; and the device can make the sampling process more standardized and avoid errors caused by improper sampling.

Description

Technical Field

[0001] This invention belongs to the field of biological detection technology, specifically a detection device for detecting Escherichia coli. Background Technology

[0002] When detecting E. coli, especially with ATP fluorescence detectors currently on the market, manual sampling is required. This involves using a sampling tube. Specifically, the user pulls out the top of the sampling tube, then fully contacts the sample with the swab on the tube (the swab rotates and slides on the sample surface). The sampling tube is then reinserted into the bottom, reagent is squeezed in, the tube is shaken well, and then placed into the detector to perform the test.

[0003] The sampling process described above is done manually by the operators. Different operators may use different methods to collect samples, which may result in insufficient sample collection and thus affect the detection results. Summary of the Invention

[0004] In view of the above situation and to overcome the shortcomings of the prior art, the present invention provides a detection device for detecting Escherichia coli, which effectively solves the problems in the background art.

[0005] To achieve the above objectives, the present invention provides the following technical solution: a detection device for detecting Escherichia coli, comprising a detector housing and a sampling tube, wherein the sampling tube has an upper and lower split structure, and the upper and lower parts of the sampling tube are interlocked with each other; a fixed frame is provided on one side of the detector housing, and a fixed rod and a movable rod are provided inside the fixed frame, with the movable rod located on one side of the fixed rod; a sample rack is also provided inside the fixed frame, and the sample rack is rotatable.

[0006] A sampling tube is placed inside the fixed frame. A first clamping block is provided on the fixed rod, and a second clamping block is provided on the movable rod. The first clamping block can clamp the lower part of the sampling tube, and the second clamping block can clamp the upper part of the sampling tube.

[0007] A connecting block is provided inside the fixed rod. The connecting block can move up and down inside the fixed rod. The movable rod is fixed to the connecting block. When the connecting block moves upward, the first clamp can clamp the outside of the sampling tube and prevent the lower end of the sampling tube from moving. The second clamp can clamp the outside of the sampling tube and make the upper end of the sampling tube move upward.

[0008] The second clamping block is equipped with a sliding structure. When the movable rod moves upward to the predetermined position, the sliding structure can drive the upper end of the sampling tube to rotate.

[0009] Preferably, a first gear is provided inside the fixed rod, and a first actuating rod arranged vertically meshes with one side of the first gear. One end of the first clamping block is fixed outside the fixed rod, and a first threaded rod is threaded onto the first clamping block. One end of the first threaded rod is inserted into the fixed rod and can rotate. A first annular tooth is provided outside the first threaded rod, and the first annular tooth meshes with the first actuating rod. An actuating rack is provided on the side of the movable rod, and the actuating rack cooperates with the first gear.

[0010] Preferably, a second threaded rod is rotatably provided inside the movable rod. The second threaded rod passes through the second clamping block and the two are threadedly engaged. A second actuating rod that can move up and down is provided inside the movable rod. A second annular tooth is provided outside the second threaded rod. The second annular tooth meshes with the second actuating rod. The second actuating rod and the movable rod are connected by a spring. When the movable rod moves upward, the second actuating rod moves downward under the action of the spring and its own weight. When the movable rod moves downward and presses against the inner lower wall of the fixed frame, the second actuating rod is pushed into the movable rod.

[0011] Preferably, the fixed rod is provided with a rotatable lead screw, the connecting block is threaded onto the outside of the lead screw, and a smooth surface is provided near the upper end of the lead screw. A second gear is fixed on the smooth surface. The distance between the threaded surface of the lead screw and the lower end face of the second gear is greater than the thickness of the connecting block. A third gear and a fourth gear are provided in the movable rod. The fourth gear is located on one side of the third gear and the two mesh with each other. When the connecting block moves outside the smooth surface of the lead screw, the third gear can mesh with the second gear.

[0012] Preferably, the sliding structure has two symmetrically arranged sliding structures, each including an annular rotating belt wrapped around two rotating shafts. The upper part of the sampling tube is fixedly clamped between the two annular rotating belts. A transmission rod is fixedly connected to the axis of the third gear. One end of a steel wire rope is fixedly connected to one of the rotating shafts, and the other end of the steel wire rope is fixedly connected to the transmission rod, so that when the transmission rod rotates, the steel wire rope can pull the annular transmission belt to rotate.

[0013] Preferably, the sample holder has an upward-opening placement slot, a push plate is provided in the placement slot, a rotatable third threaded rod is provided in the sample holder, the third threaded rod is threadedly engaged with the push plate, and a through hole is provided on the side wall of the placement slot.

[0014] Preferably, the upper end of the detector housing is hinged to a first housing, and the upper end of the fixing frame is hinged to a second housing.

[0015] Compared with the prior art, the beneficial effects of the present invention are:

[0016] In this device, the upper and lower parts of the sampling tube can be separated by driving the connecting block. After the upper part of the sampling tube moves to the predetermined position, the upper part of the sampling tube stops moving upward as the connecting block continues to move upward. At this time, the sample is placed on the sample holder and pushed into the fixed frame. The swab inside the sampling tube can fully contact the sample surface to achieve the purpose of sampling. Then, the sampling tube is placed into the detector to detect E. coli in the sample. The setup of this device can more standardize the sampling process and avoid errors caused by human sampling. Attached Figure Description

[0017] The accompanying drawings are provided to further illustrate the invention and form part of the specification. They are used in conjunction with embodiments of the invention to explain the invention and do not constitute a limitation thereof. In the drawings:

[0018] Figure 1 This is a schematic diagram of the overall front view of the present invention;

[0019] Figure 2 This is a schematic diagram of the internal front view of the fixing frame of the present invention;

[0020] Figure 3 This is a schematic diagram of the cooperation structure between the fixed rod and the movable rod of the present invention;

[0021] Figure 4 This is a schematic diagram of one side of the fixing rod of the present invention;

[0022] Figure 5 This is a schematic diagram of one side of the movable rod of the present invention;

[0023] Figure 6 This is a top view of the movable rod of the present invention.

[0024] Figure 7 For the present invention Figure 6 A magnified schematic diagram of the central part of the structure;

[0025] Figure 8 This is a schematic diagram of the internal structure of the sample holder of the present invention.

[0026] In the diagram: 1. Detector housing; 2. First top cover; 3. Fixing frame; 4. Second top cover; 5. Sampling tube; 6. First clamping block; 7. Second clamping block; 8. Fixing rod; 9. Movable rod; 10. Connecting block; 11. First gear; 12. First actuating rod; 13. First threaded rod; 14. Lead screw; 15. Second gear; 16. Second threaded rod; 17. Second actuating rod; 18. Transmission rod; 19. Third gear; 20. Fourth gear; 21. Wire rope; 22. Circular rotating belt; 23. Sample holder; 24. Push plate; 25. Third threaded rod; 26. Through hole. Detailed Implementation

[0027] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are within the scope of protection of the present invention.

[0028] Depend on Figures 1-8 The present invention discloses a detection device for detecting Escherichia coli, comprising a detector housing 1 and a sampling tube 5. The sampling tube 5 has a split structure, and the upper and lower parts of the sampling tube 5 are interlocked (which is an existing structural style). A fixing frame 3 is provided on one side of the detector housing 1. A fixing rod 8 and a movable rod 9 are provided inside the fixing frame 3, and the movable rod 9 is located on one side of the fixing rod 8. A sample loading rack 23 is also provided inside the fixing frame 3. The sample loading rack 23 can rotate, and the sample is placed into the sample loading rack 23, which facilitates the sample loading process.

[0029] A sampling tube 5 is placed inside the fixing frame 3. A first clamping block 6 is provided on the fixing rod 8, and a second clamping block 7 is provided on the movable rod 9. The first clamping block 6 can clamp the lower part of the sampling tube 5, and the second clamping block 6 can clamp the upper part of the sampling tube 5. The first clamping block 6 and the second clamping block 7 can clamp different positions of the sampling tube 5, thereby allowing the sampling tube 5 to be separated, which facilitates the subsequent sampling steps.

[0030] There are two first clamping blocks 6 and two second clamping blocks 7, and they are symmetrically arranged. The first clamping blocks 6 and the second clamping blocks 7 are made of elastic material (e.g., steel sheet / plastic sheet) so that their ends can move away from or close to each other.

[0031] A connecting block 10 is provided inside the fixed rod 8. The connecting block 10 can move up and down inside the fixed rod 8. The movable rod 9 is fixed to the connecting block 10. When the connecting block 10 moves upward, the first clamping block 6 can clamp the outside of the sampling tube 5 and prevent the lower end of the sampling tube 5 from moving. The second clamping block 7 can clamp the outside of the sampling tube 5 and make the upper end of the sampling tube 5 move upward.

[0032] The second clamping block 7 is provided with a sliding structure. When the movable rod 9 moves upward to the predetermined position, the sliding structure can drive the upper end of the sampling tube 5 to rotate.

[0033] When this device is implemented, the upper and lower parts of the sampling tube 5 can be separated by driving the connecting block 10. After the upper part of the sampling tube 5 moves to the predetermined position, the upper part of the sampling tube 5 will no longer move upward as the connecting block 10 continues to move upward. At this time, the sample is placed on the sample holder 23 and pushed into the fixing frame 3. The swab in the sampling tube 5 can fully contact the sample surface to achieve the purpose of sampling. Then, the sampling tube 5 is placed into the detector to detect E. coli in the sample. The setup of this device can more standardize the sampling process and avoid errors caused by human sampling.

[0034] The fixed rod 8 is internally provided with a first gear 11, and a vertically arranged first actuating rod 12 meshes with one side of the first gear 11. One end of the first clamping block 6 is fixed to the outside of the fixed rod 8, and a first threaded rod 13 is threadedly sleeved on the first clamping block 6. One end of the first threaded rod 13 is inserted into the fixed rod 8 and can rotate. A first annular tooth is provided on the outside of the first threaded rod 13, and the first annular tooth meshes with the first actuating rod 12. When the first gear 11 rotates, the first gear 11 drives the first actuating rod 12 to slide, and the first actuating rod 12 can drive the end of the first clamping block 6 to slide, thereby realizing the clamping or separation operation. A actuating rack is provided on the side of the movable rod 9, and the actuating rack cooperates with the first gear 11.

[0035] The above-mentioned technical solution enables the device to operate so that when the movable rod 9 moves downward, the actuating rack on the side of the movable rod 9 engages with the first gear 11, thereby allowing the first clamping blocks 6 to move away from each other, thus facilitating the user to place or remove the sampling tube 5 into or from the fixing frame 3.

[0036] To ensure that when the movable rod 9 is in its lowest position (the lower end of the movable rod 9 rests against the inner lower wall of the fixed frame 3), the ends of the second clamping blocks 7 can move away from each other, while when the movable rod 9 moves upward, the ends of the second clamping blocks 7 can move closer to each other (clamping), the following technical solution is provided: A second threaded rod 16 is rotatably provided inside the movable rod 7. The second threaded rod 16 passes through the second clamping block 7 and the two are threaded together. A second actuating rod 17 that can move up and down is provided inside the movable rod 9. A second annular tooth is provided outside the second threaded rod 16. The second annular tooth meshes with the second actuating rod 17, and the second actuating rod 17 is connected to the movable rod 9 by a spring. When the movable rod 9 moves upward, the second actuating rod 17 moves downward under the action of the spring 17 and its own weight. When the movable rod 9 moves downward and rests against the inner lower wall of the fixed frame 3, the second actuating rod 17 is pushed into the movable rod 9.

[0037] The fixed rod 8 is equipped with a rotatable lead screw 14. The connecting block 10 is threaded onto the lead screw 14. The lead screw 14 has a smooth surface near its upper end. A second gear 15 is fixed on the smooth surface. The distance between the threaded surface of the lead screw 14 and the lower end face of the second gear 15 is greater than the thickness of the connecting block 10. A third gear 19 and a fourth gear 20 are provided in the movable rod 9. The fourth gear 20 is located on one side of the third gear 19 and the two mesh with each other. When the connecting block 10 moves outside the smooth surface of the lead screw 14, the third gear 19 can mesh with the second gear 15.

[0038] The design of the lead screw 14 not only allows the connecting block 10 to move up and down when it rotates, but also allows the connecting block 10 to vibrate up and down to a certain extent in the vertical direction after it moves up to the smooth surface due to the support effect of the thread tip on the connecting block 10. This further ensures that the swab and the sample are in full contact during sampling.

[0039] The sliding structure has two symmetrically arranged components, each including an annular rotating belt 22 wound around two rotating shafts. The upper part of the sampling tube 5 is fixedly clamped between the two annular rotating belts 22. A transmission rod 18 is fixedly connected to the axis of the third gear 19. One end of a steel wire rope is fixedly connected to one of the rotating shafts, and the other end of the steel wire rope is fixedly connected to the outside of the transmission rod 18, so that when the transmission rod 18 rotates, the steel wire rope can pull the annular transmission belt 22 to rotate.

[0040] The sliding structure allows the sampling tube held within it to rotate, meaning the cotton swab below the sampling tube rotates on the sample surface (and can also move up and down), thus ensuring sufficient contact during sampling.

[0041] The sample holder 23 has an upward-opening placement slot, a push plate 24 is provided in the placement slot, and a rotatable third threaded rod 25 is provided in the sample holder 23. The third threaded rod 25 is threadedly engaged with the push plate 24, and a through hole 26 is provided on the side wall of the placement slot.

[0042] After the sample is placed into the placement slot, the pusher plate 24 can push the sample into the holder 3, and some samples (such as softer samples) can pass through the through hole and extend into the holder 3, thus coming into contact with the cotton swab. In some embodiments, the through hole 26 may not be provided.

[0043] Of course, in order to ensure that the sample can be pushed to the predetermined position, a glass window can be set at the corresponding position of the fixing frame 3 so that the user can observe it.

[0044] The upper end of the detector housing 1 is hinged to a first housing 2, and the upper end of the fixing frame 3 is hinged to a second housing 4.

[0045] The lead screw 14 can be driven by a motor.

[0046] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0047] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A detection device for detecting Escherichia coli, comprising a detector housing (1) and a sampling tube (5), wherein the sampling tube (5) has an upper and lower split structure, and the upper and lower parts of the sampling tube (5) are interlocked, characterized in that: A fixed frame (3) is provided on one side of the detector housing (1). A fixed rod (8) and a movable rod (9) are provided inside the fixed frame (3), and the movable rod (9) is located on one side of the fixed rod (8). A sample rack (23) is also provided inside the fixed frame (3), and the sample rack (23) can rotate. A sampling tube (5) is placed inside the fixed frame (3). A first clamping block (6) is provided on the fixed rod (8), and a second clamping block (7) is provided on the movable rod (9). The first clamping block (6) can clamp the lower part of the sampling tube (5), and the second clamping block (7) can clamp the upper part of the sampling tube (5). A connecting block (10) is provided inside the fixed rod (8). The connecting block (10) can move up and down inside the fixed rod (8). The movable rod (9) is fixed to the connecting block (10). When the connecting block (10) moves upward, the first clamping block (6) can clamp the sampling tube (5) and prevent the lower end of the sampling tube (5) from moving. The second clamping block (7) can clamp the sampling tube (5) and make the upper end of the sampling tube (5) move upward. The second clamp (7) is provided with a sliding structure. When the movable rod (9) moves upward to the predetermined position, the sliding structure can drive the upper end of the sampling tube (5) to rotate. The fixed rod (8) is provided with a first gear (11) inside. A first actuating rod (12) arranged vertically is meshed on one side of the first gear (11). One end of the first clamping block (6) is fixed outside the fixed rod (8), and a first threaded rod (13) is threaded onto the first clamping block (6). One end of the first threaded rod (13) is inserted into the fixed rod (8), and the first threaded rod (13) can rotate. A first ring tooth is provided outside the first threaded rod (13), and the first ring tooth meshes with the first actuating rod (12). A actuating rack is provided on the side of the movable rod (9), and the actuating rack cooperates with the first gear (11). The movable rod (7) is rotatably provided with a second threaded rod (16), which passes through the second clamp (7) and the two are threaded together. The movable rod (9) is provided with a second actuating rod (17) that can move up and down. The second threaded rod (16) is provided with a second ring tooth, which meshes with the second actuating rod (17). The second actuating rod (17) and the movable rod (9) are connected by a spring. When the movable rod (9) moves upward, the second actuating rod (17) moves downward under the action of the spring (17) and its own weight. When the movable rod (9) moves downward and hits the inner lower wall of the fixed frame (3), the second actuating rod (17) is pushed into the movable rod (9). The fixed rod (8) is provided with a rotatable lead screw (14), and the connecting block (10) is threaded onto the outside of the lead screw (14). The lead screw (14) is provided with a smooth surface near the upper end, and a second gear (15) is fixed on the smooth surface. The distance between the threaded surface of the lead screw (14) and the lower end surface of the second gear (15) is greater than the thickness of the connecting block (10). A third gear (19) and a fourth gear (20) are provided in the movable rod (9). The fourth gear (20) is located on one side of the third gear (19) and the two mesh with each other. When the connecting block (10) moves to the outside of the smooth surface of the lead screw (14), the third gear (19) can mesh with the second gear (15). The sliding structure has two symmetrically arranged sliding structures, including an annular rotating belt (22), which is wrapped around two rotating shafts. The upper part of the sampling tube (5) is fixedly clamped between the two annular rotating belts (22). A transmission rod (18) is fixedly connected to the axis of the third gear (19). One end of a steel wire rope is fixedly connected to one of the rotating shafts, and the other end of the steel wire rope is fixedly connected to the outside of the transmission rod (18) so that when the transmission rod (18) rotates, the steel wire rope can pull the annular transmission belt (22) to rotate.

2. The detection device for detecting Escherichia coli according to claim 1, characterized in that: The sample holder (23) has an upward-opening placement slot, a push plate (24) is provided in the placement slot, and a rotatable third threaded rod (25) is provided in the sample holder (23). The third threaded rod (25) is threadedly engaged with the push plate (24), and a through hole (26) is provided on the side wall of the placement slot.

3. The detection device for detecting Escherichia coli according to claim 1, characterized in that: The upper end of the detector housing (1) is hinged to a first housing (2), and the upper end of the fixing frame (3) is hinged to a second housing (4).

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