一种叶黄素脂肪酸单酯的提取分离方法及其应用

By employing specific solvent crystallization and lipase hydrolysis, the problem of lutein monoester purification has been solved, enabling the preparation of high-purity lutein fatty acid monoesters suitable for nutritional supplements and food additives, thus improving product stability and bioavailability.

CN121496029BActive Publication Date: 2026-07-17HANGZHOU NORMAL UNIVERSITY +3

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
HANGZHOU NORMAL UNIVERSITY
Filing Date
2025-10-29
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

The lack of efficient methods for purifying and separating lutein monoesters in existing technologies results in low bioavailability and poor stability in the human body, failing to meet the needs of those with rapid absorption or weak digestive and absorptive functions.

Method used

By employing a specific solvent for the first cooling crystallization, combined with a lipase hydrolysis reaction and a second cooling crystallization, and by controlling the reaction conditions and washing steps, the purity and yield of lutein fatty acid monoesters can be improved.

Benefits of technology

High-purity extraction of lutein fatty acid monoesters was achieved, with a purity of over 70%, making it suitable as a nutritional supplement or food additive, and exhibiting good stability and bioavailability.

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Abstract

本发明提供了一种叶黄素脂肪酸单酯的提取分离方法及其应用,属于提纯领域。本发明提供了一种叶黄素脂肪酸单酯的提取分离方法,包括以下步骤:将含叶黄素脂肪酸酯的植物油树脂溶解于液态烷烃后将所得植物油树脂的溶液与第一醇溶剂混合后再进行第一降温结晶,然后将所得体系进行第一分离,再对所得湿固体进行干燥,得到叶黄素脂肪酸酯膏状物;将所述叶黄素脂肪酸酯膏状物、第二醇溶剂与脂肪酶的分散液混合进行水解反应,生成叶黄素脂肪酸单酯,得到反应液;将所述反应液进行第二分离,然后将所得液相去除部分溶剂后进行第二降温结晶,将所得结晶体系进行第三分离,而后将所得固体进行后处理,得到纯叶黄素脂肪酸单酯。
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