A device for cryopreservation of a cell preparation

Abstract

This invention relates to the field of human local preservation technology, specifically to a cryopreservation device for cell products, comprising an incubator and a passive cooling module, an active cooling module, and a control module disposed therein. The passive cooling module includes several sealed cartridges, each pre-filled with a phase change material, evenly distributed circumferentially and detachably connected to the incubator. The active cooling module includes a circulating air duct and a cooler and a fan disposed within the circulating air duct. The two ends of the circulating air duct are connected to the top and bottom of the incubator, respectively, and the cold air blown out by the circulating air duct is evenly introduced into the incubator from the top circumferentially. The control module includes a controller and a temperature sensor, with the temperature sensor, cooler, and fan all electrically connected to the controller. This invention enables high uniformity and stability of temperature control within the device, effectively suppressing temperature fluctuations and spatial temperature differences, thereby meeting the precise temperature maintenance requirements of cells during cryopreservation.

Description

Technical Field

[0001] This invention relates to the field of human body local preservation technology, specifically to a cryopreservation device for cell products. Background Technology

[0002] Cell products refer to biological agents with living cells as the core active ingredient, which are isolated, cultured, expanded, or genetically modified in vitro for use in disease treatment, tissue repair, or regenerative medicine, such as CAR-T cells, mesenchymal stem cells, and pancreatic islet cells. Because they are directly derived from human tissues or individuals, cell products are considered, from a legal and bioethical perspective, an ex vivo form of human tissue components, belonging to "local parts of the human body" or their functional derivatives. Unlike traditional chemical drugs, cell products have characteristics such as activity dependence, temperature sensitivity, and short time windows. Their efficacy and safety are highly dependent on the cell survival rate, functional integrity, and sterility during preservation and transportation. Therefore, the transportation of cell products is not merely a physical displacement, but also a precise maintenance of the preservation environment of locally active tissues derived from the human body throughout the entire process.

[0003] Temperature control is the core factor determining the quality and efficacy of cell products during preservation and transportation. Different types of cell products have extremely strict and significant temperature requirements: (1) Low-temperature refrigerated type (2~8℃): such as some mesenchymal stem cell suspensions, CAR-T cell infusion pre-treatment products, etc., need to be maintained in this temperature range for several hours to several days. Too high a temperature (>8℃) will accelerate cell metabolism, leading to acidosis, apoptosis or functional decline; too low a temperature (≤0℃) may cause ice crystal formation, resulting in cell membrane rupture. (2) Room temperature stable type (15~25℃): Some cell products protected by special formulas may allow short-term room temperature preservation and transportation, but the temperature range must be maintained within the time window (usually <4 hours).

[0004] Based on this, existing technology (Chinese invention patent application publication number CN111543423A) discloses an intelligent portable multifunctional cell or tissue cryopreservation and transport device, including a device body, which includes a lid and a body, and the body includes an outer box and an inner box. Insulation material is pre-installed between the outer and inner boxes. An evaporation module, a condensation module, and a battery are pre-installed between the bottom surface of the inner box and the bottom surface of the outer box. The battery is electrically connected to the evaporation and condensation modules. The evaporation and condensation modules are isolated by a first insulation layer. A refrigerant placement layer is provided on the side of the inner box. A second insulation layer isolates the lid and body. In use, utilizing the principle of evaporative heat absorption, the evaporation module delivers cold energy into the box, and the condensation module transfers heat to the environment, achieving active cooling. When the active cooling system cannot operate, passive cooling is used, placing pre-frozen refrigerant in the refrigerant placement layer inside the box, utilizing the refrigerant's melting and phase change heat absorption.

[0005] Although the aforementioned existing technologies can achieve the functions of low-temperature preservation and transportation of cell products to a certain extent, there are still technical problems in practical applications, such as uneven temperature distribution inside the chamber and unstable and large fluctuations in temperature. Summary of the Invention

[0006] The purpose of this invention is to provide a cryopreservation device for cell products, which partially solves or alleviates the above-mentioned deficiencies in the prior art. It can achieve high uniformity and high stability control of temperature within the device, effectively suppress temperature fluctuations and spatial temperature differences, thereby meeting the requirement for precise maintenance of a strict temperature control environment throughout the cryopreservation and transportation process of cell products.

[0007] To solve the aforementioned technical problems, the present invention specifically adopts the following technical solution: A cell product cryopreservation device includes an insulated box and a passive refrigeration module, an active refrigeration module, and a control module disposed within the insulated box. The insulated box includes a box body and an openable lid that covers the top of the box body. The box body includes an outer shell and an inner liner connected to the outer shell. A side insulation layer is disposed circumferentially between the outer shell and the inner liner. The passive refrigeration module includes several sealing cartridges, each pre-filled with a phase change material. The several sealing cartridges are evenly disposed circumferentially within the inner liner, and the sealing cartridges are detachably connected to the inner wall of the inner liner. The active refrigeration module includes a circulating air duct and a cooler and a fan disposed within the circulating air duct. The two ends of the circulating air duct are respectively connected to the top and bottom of the inner liner, and the cold air blown out by the circulating air duct is evenly introduced into the inner liner from the top circumferentially. The control module includes a controller and a temperature sensor, the temperature sensor being located within the inner liner. The temperature sensor, the cooler, and the fan are all electrically connected to the controller. The phase change material pre-filled in the sealed cartridge is used for passive cooling through phase change heat absorption; the temperature sensor is used to detect the temperature inside the inner liner and send a temperature detection signal to the controller; when the temperature detection signal shows that the temperature inside the inner liner is higher than a threshold, the controller is used to control the refrigerator and the fan to start according to the temperature detection signal, and at the same time perform active cooling.

[0008] Preferably, as an improvement, the circulating air duct includes an installation cavity, an air distribution cavity, and a return cavity, with the installation cavity connecting the air distribution cavity and the return cavity; an installation cover is sealed to the outer side wall of the outer shell, the space between the installation cover and the outer shell forming the installation cavity, and the cooler and the fan are disposed within the installation cavity; the interior of the cover is hollow to form an air distribution cavity, and a plurality of air distribution holes are evenly distributed along the circumference of the inner liner on the inner wall of the cover, the air distribution holes communicating with the air distribution cavity for introducing cold air from the air distribution cavity into the inner liner; a return cover is sealed to the bottom of the outer shell, the space between the return cover and the outer shell forming a return cavity, and at least one return hole is provided at the bottom of the box, the two ends of the return hole communicating with the inner liner and the return cavity respectively, for introducing the cold air after heat exchange in the inner liner into the return cavity; And / or, the air distribution hole is a strip-shaped hole, and the length direction of the air distribution hole is parallel to the direction of the corresponding inner liner sidewall; And / or, the bottom of the housing is provided with a plurality of the aforementioned return holes, and the return holes are strip-shaped holes, and the return holes and the air distribution holes are arranged in parallel one-to-one; And / or, a plurality of air guide plates are provided on the inner wall of the lid, the air guide plates are arranged one-to-one on the side of the air distribution hole away from the inner liner, and the bottom end of the air guide plate is biased toward the corresponding side wall of the inner liner, for guiding the cold air blown out of the air distribution hole to the inner side wall of the inner liner.

[0009] Preferably, as an improvement, the top of the mounting cover is provided with a communication hole communicating with the mounting cavity, and the inner wall of the box cover is provided with an air inlet communicating with the air distribution cavity. The communication hole and the air inlet are positioned opposite to each other and communicate with each other, for connecting the mounting cavity and the air distribution cavity. And / or, the inner wall of the box cover is sealed with an air intake channel, the air intake channel surrounds the air intake hole therein, the air intake channel extends into the mounting cavity through the connecting hole, and the outer wall of the air intake channel slides in fit with the inner wall of the connecting hole; And / or, the reflux hood has an air outlet on its side wall near the mounting hood, and the mounting hood covers the air outlet therein, with the two ends of the air outlet communicating with the mounting cavity and the reflux cavity, respectively.

[0010] Preferably, as an improvement, an annular partition plate is provided in the air distribution cavity along the circumference of the inner liner. The two ends of the partition plate are respectively sealed to the top and bottom of the air distribution cavity, dividing the air distribution cavity into an annular shape. The space inside the partition plate forms an isolation cavity, and a top insulation layer is provided in the isolation cavity. And / or, the sealing cartridge is detachably connected to the inner wall of the box cover, and the sealing cartridge is pre-filled with phase change material.

[0011] Preferably, as an improvement, the passive cooling module further includes a thermally conductive and flow-limiting layer, which is laid on the inner side wall of the inner liner. A connecting groove is provided on the top of the thermally conductive and flow-limiting layer. The width of the connecting groove is at least able to accommodate one of the sealing cartridges, and the sealing cartridge is inserted into the connecting groove. And / or, limiting blocks are provided on the parallel side walls of the connecting groove, the limiting blocks dividing the connecting groove into two receiving spaces along the thickness direction of the thermally conductive and current-limiting layer, each receiving space being able to insert one of the sealing cartridges; one or two sealing cartridges can be inserted into the same connecting groove, and when two sealing cartridges are inserted, the temperature of the phase change material in the inner sealing cartridge is lower than the temperature of the phase change material in the outer sealing cartridge.

[0012] Preferably, as an improvement, the cooler is a semiconductor cooler, which is embedded in the side wall of the mounting cover; the cold end of the semiconductor cooler is provided with a cold end heat sink, which is located inside the mounting cover and is used to exchange heat with the air in the mounting cavity; the hot end of the semiconductor cooler is provided with a hot end heat sink, which is located outside the mounting cover and is used to exchange heat with the external environment. And / or, when the temperature detection signal indicates that the temperature inside the inner liner is lower than the threshold, the controller is used to control the semiconductor cooler and the fan to start according to the temperature detection signal, and to perform active heating.

[0013] Preferably, as an improvement, it also includes a liquid collection module, which includes a liquid collection drawer; the side wall of the reflux hood is provided with a pull-out opening communicating with the reflux chamber, the liquid collection drawer is slidably inserted into the pull-out opening, and the horizontal projection of the reflux hole is located within the range of the liquid collection drawer; And / or, a sealing element is provided between the liquid collection drawer and the pull-out opening; And / or, the liquid collection module further includes a guide plate, which is connected to the side wall of the inner liner with a gap by connecting bolts, and the air distribution hole and the return hole are located between the guide plate and the side wall of the inner liner; the top of the guide plate is bent inward, and a gap is left between the bottom of the guide plate and the bottom of the inner liner; And / or, at least one heat exchange hole is provided on the guide plate.

[0014] Preferably, as an improvement, it further includes a sample support frame, the sample support frame comprising a tray and a support member; the support member is connected between the bottom of the tray and the bottom of the inner liner, the tray being used to hold a container containing the cell product; And / or, a liquid collection groove is provided on the inner bottom side of the tray along the circumferential direction, and a drip nozzle is provided on the outer bottom side of the tray, the drip nozzle being in communication with the liquid collection groove; And / or, the support member is a shock absorber.

[0015] Preferably, as an improvement, the control module further includes a mounting bracket disposed within the inner liner, the mounting bracket comprising a telescopic rod and a flexible connecting rod with a fixed posture; the bottom end of the telescopic rod is detachably connected to the inner liner, the top end of the telescopic rod is fixedly connected to the flexible connecting rod, and the temperature sensor is connected to the free end of the flexible connecting rod; the telescopic rod is used to adjust the height of the temperature sensor, and the flexible connecting rod is used to adjust the spatial position of the temperature sensor, so that the temperature sensor is close to the cell product; And / or, the control module further includes a switch button, a temperature adjustment button, and a display screen disposed on the outer wall of the insulation box. The switch button, the temperature adjustment button, and the display screen are all electrically connected to the controller. The switch button is used to turn the active cooling module and the control module on and off. The temperature adjustment button is used to set the storage temperature. The display screen is used to display the set temperature and / or the actual temperature detected by the temperature sensor.

[0016] Preferably, as an improvement, one side of the lid is hinged to the corresponding position of the outer shell via a hinge, and a latch is provided on the non-hinged side of the lid and the corresponding position of the outer shell; And / or, the top of the lid is provided with a handle; And / or, a sealing strip is provided circumferentially on the top of the inner liner.

[0017] Beneficial technical effects of the present invention: This invention provides a cryopreservation device for cell products, applicable not only to cryopreservation of cell products in static storage conditions but also to cryopreservation of cell products during dynamic transport. Addressing the common problems of uneven temperature distribution and drastic temperature fluctuations in existing cell product cryopreservation and transport devices, this invention proposes a synergistic temperature control strategy based on passive cooling with phase change materials and assisted by active regulation with a thermoelectric cooler (TEC).

[0018] This invention abandons the traditional high-disturbance mode that relies on compressor refrigeration. Instead, it utilizes the physical property of phase change materials (PCMs) to absorb or release latent heat during phase transitions at specific temperature points (e.g., 5°C or 20°C). Under conditions of no external energy or limited power supply, it maintains the internal environment of the chamber within the required temperature control window (e.g., 2~8°C or 15~25°C) for extended periods, allowing the cell products to operate within this temperature range. Through the plateau effect of its solid-liquid phase transition process, the PCM provides a relatively stable temperature output over a wide time range, effectively buffering external thermal disturbances and preventing damage to cell viability from sudden temperature rises or drops. Simultaneously, since it eliminates the need to maintain high-power refrigeration equipment, this passive temperature control method significantly reduces energy consumption and system complexity, improving the reliability and practicality of the device in long-distance transportation, field transport, or emergency scenarios. This constructs a low-power, long-lasting, and highly stable basic constant-temperature platform.

[0019] However, purely passive systems may still experience brief overshoot or slow drift in scenarios such as the early or late stages of phase transition, frequent opening of the chamber, or sudden changes in ambient temperature. For example, the temperature inside the chamber may briefly exceed the target range when the phase change material first begins to absorb heat, or the temperature may slowly rise as the cooling capacity decays near the end of the phase transition. While these small but persistent deviations may not cause the temperature to become completely out of control, they are enough to trigger abnormal cell metabolism, functional decline, or even apoptosis, making it difficult to meet the stringent requirements of cell products that are highly sensitive to temperature fluctuations.

[0020] To address this, this invention innovatively introduces a thermoelectric cooler (TEC) as a low-power, high-response dynamic compensation unit, forming a closed-loop feedback system with a temperature sensor and controller. This system can monitor temperature changes at key locations within the inner liner in real time and make judgments based on preset thresholds: when a temperature deviation from the target window (e.g., 2~8℃) is detected, the controller immediately drives the TEC to perform micro-cooling (cooling down) or reverse current heating (heating up) to precisely offset thermal deviations caused by external thermal disturbances, ventilation from opening the lid, or nonlinear phase change release. Because the TEC has advantages such as no moving parts, rapid response, precise temperature control, and support for bidirectional adjustment, it only requires short-term, low-power operation to achieve "fine-tuning" rather than "main control." This avoids the severe oscillations caused by traditional compressor start-stop cycles and effectively controls overall temperature fluctuations within ±0.5℃, significantly improving the stability and reliability of temperature control during cell product preservation and transportation, truly realizing a high-order temperature control strategy of "passive constant temperature as the main method and active compensation as a supplementary method."

[0021] Building upon this foundation, the device constructs a closed-loop airflow circulation around the sample through a top annular air distribution chamber, circumferentially distributed air distribution holes, and bottom return holes positioned one-to-one with the air distribution holes. This ensures the uniform diffusion of the cooling energy released by the phase change material and the adjustment amount compensated by the thermoelectric cooler (TEC), effectively eliminating the vertical and horizontal temperature differences caused by natural convection in traditional devices and ensuring that samples at multiple locations are always in a highly consistent thermal environment. To further optimize airflow organization, a guide plate is also installed on the inner wall of the chamber lid, with its bottom end inclined towards the inner liner sidewall. This guides the airflow blown from the air distribution holes to the circumferential surface of the inner liner, preventing cold air from directly blowing onto the sample and causing localized overcooling. Simultaneously, a narrow annular air channel is formed between the guide plate and the inner liner sidewall. Combined with the directional suction of the bottom return holes, this guides the airflow to descend smoothly along the periphery of the sample, then returns to the top air distribution chamber via the return chamber and mounting chamber, forming a stable, low-speed, and fully covered circulation path. This design not only enhances the uniformity of the temperature field but also reduces the direct impact of airflow disturbances on the cell product container, balancing temperature control accuracy and sample safety.

[0022] Meanwhile, the entire unit integrates functional modules such as liquid collection and anti-condensation, shock absorption and support, adjustable temperature measurement, sealing and locking, and human-computer interaction. While ensuring temperature control performance, it also considers sterility, safety, and ease of operation, fully meeting the stringent requirements of cell therapy products for maintaining activity, functional integrity, and compliance with Good Manufacturing Practices (GMP) during storage and transportation. Specifically, the liquid collection module, through a pull-out collection drawer and guide plate within the bottom reflux hood, efficiently collects condensate caused by temperature differences, preventing condensate from dripping onto the sample labels and damaging them. The sample support frame uses a combination design of shock-absorbing supports and a tray with a collection trough, which not only buffers vibrations and impacts during transportation but also guides accidentally leaked liquid to the dropper for centralized discharge, preventing secondary contamination and ensuring sample safety. The temperature sensor achieves three-dimensional spatial position adjustment through a telescopic rod and a flexible connecting rod, allowing it to closely adhere to the actual loaded cell product container, ensuring that the temperature measurement point accurately reflects the sample's microenvironment and improving the accuracy and timeliness of temperature control feedback.

[0023] In summary, this invention, through a multi-level synergistic mechanism of "passive constant temperature + active fine-tuning + airflow temperature equalization," achieves high-precision, high-uniformity, and high-stability control of the cryopreservation and transport environment for cell products under the premise of low energy consumption and high robustness. It effectively solves the core pain points of existing technologies and has significant technological progress and clinical application value. Attached Figure Description

[0024] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. In all the drawings, similar elements or parts are generally identified by similar reference numerals. The elements or parts in the drawings are not necessarily drawn to scale. Obviously, the drawings described below are some embodiments of the present invention, and those skilled in the art can obtain other drawings based on these drawings without any creative effort.

[0025] Figure 1 This is a schematic diagram of the overall structure of the box lid being closed, provided in an embodiment of the present invention. Figure 2 This is a schematic diagram of the overall structure of the liquid collection drawer sliding out according to an embodiment of the present invention; Figure 3 This is a schematic diagram of the overall structure of the box lid opening according to an embodiment of the present invention; Figure 4 This is a schematic diagram of the overall structure of the box cover opening from another perspective, provided by an embodiment of the present invention; Figure 5 A top view of the box lid being opened according to an embodiment of the present invention (the box lid is not shown); Figure 6A cross-sectional view along the length of the insulation box provided in an embodiment of the present invention; Figure 7 for Figure 6 Schematic diagram of the structure at point A; Figure 8 for Figure 6 A schematic diagram of the three-dimensional structure; Figure 9 for Figure 6 A schematic diagram of the three-dimensional structure from another perspective; Figure 10 This is a cross-sectional view along the width of the insulation box provided in an embodiment of the present invention.

[0026] Summary of attached labeling and identification: 1. Insulated Box; 11. Box Body; 111. Outer Shell; 112. Inner Liner; 113. Side Insulation Layer; 114. Box Cover; 12. Air Guide Plate; 121. Isolation Plate; 122. Isolation Chamber; 123. Top Insulation Layer; 124. Hinge; 13. Sealing Strip; 14. Mounting Cover; 15. Return Flow Cover; 16. Passive Cooling Module; 2. Sealing Casing; 21. Thermal Conduction and Flow Restriction Layer; 22. Connecting Groove; 221. Limiting Block; 222. Active Cooling Module; 3. Circulating Air Duct; 31. Mounting Chamber; 311. Air Distribution Chamber; 312. Return Flow Chamber; 313. Air Distribution Hole; 314. Return Flow Hole; 315. Connecting Hole; 316. Air Inlet; 317. Air Inlet Channel 318, air outlet 319, cooler 32, semiconductor cooler 321, cold end heat sink 322, hot end heat sink 323, fan 33, mounting sleeve 34, control module 4, controller 41, temperature sensor 42, mounting bracket 43, telescopic rod 431, flexible connecting rod 432, switch button 44, temperature adjustment button 45, display screen 46, liquid collection module 5, liquid collection drawer 51, pull-out port 52, guide plate 53, connecting bolt 54, heat exchange hole 55, sample support bracket 6, tray 61, liquid collection tank 611, dropper 612, support component 62. Detailed Implementation

[0027] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of the present invention. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are within the scope of protection of the present invention.

[0028] In this document, suffixes such as "module," "part," or "unit" used to denote elements are used only for the purpose of illustrative purposes and have no specific meaning in themselves. Therefore, "module," "part," or "unit" may be used interchangeably.

[0029] In this document, the terms "upper," "lower," "inner," "outer," "front," "rear," "one end," and "the other end," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are used only for the convenience of describing the present invention and for simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0030] In this document, unless otherwise explicitly specified and limited, the terms "installed," "equipped with," "connected," etc., should be interpreted broadly. For example, "connection" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection, a direct connection, or an indirect connection through an intermediate medium; it can be a connection within two components. Those skilled in the art can understand the specific meaning of the above terms in this invention based on the specific circumstances.

[0031] In this document, "and / or" includes any and all combinations of one or more of the listed related items.

[0032] In this article, "several" and "multiple" refer to two or more, that is, including two, three, four, five, etc.

[0033] As used in this specification, the term "about" typically means + / -5% of the value, more typically + / -4% of the value, more typically + / -3% of the value, more typically + / -2% of the value, even more typically + / -1% of the value, and even more typically + / -0.5% of the value.

[0034] In this specification, certain embodiments may be disclosed in a range-bound format. It should be understood that this "range-bound" description is merely for convenience and brevity and should not be construed as a rigid limitation on the disclosed range. Therefore, the description of a range should be considered as having specifically disclosed all possible subranges and the individual numerical values ​​within those ranges. For example, a description of the range 1-6 should be considered as having specifically disclosed subranges such as from 1 to 3, from 1 to 4, from 1 to 5, from 2 to 4, from 2 to 6, from 3 to 6, etc., and the individual numbers within those ranges, such as 1, 2, 3, 4, 5, and 6. This rule applies regardless of the breadth of the range.

[0035] Definition of noun: A thermoelectric cooler (TEC) is a device that generates cooling by utilizing the thermoelectric effect (also known as the Peltier effect) of semiconductor materials. When a direct current flows through a circuit composed of two different semiconductor materials (usually P-type and N-type bismuth telluride), heat transfer occurs at the junction. One end absorbs heat (cooling), and the other end releases heat (heating). Reversing the polarity of the DC power supply reverses the direction of heat flow, thus switching between cooling and heating modes.

[0036] Example 1: This embodiment provides a cryopreservation device for cell products, as shown in the attached document. Figures 1 to 10 As shown, the low-temperature storage device includes an insulated box 1 and a passive refrigeration module 2, an active refrigeration module 3, and a control module 4 installed inside the insulated box 1.

[0037] The insulated box 1 includes a box body 11 and a lid 12 that can be opened and closed onto the top of the box body 11. The box body 11 includes an outer shell 111 and an inner liner 112 connected inside the outer shell 111. A side insulation layer 113 is provided circumferentially between the outer shell 111 and the inner liner 112. Specifically, one side of the lid 12 is hinged to the corresponding position of the outer shell 111 via a hinge 13; the top of the inner liner 112 extends outward and overlaps the top of the outer shell 111, and is fixed to the outer shell 111 by welding or other means. The material of the side insulation layer 113 can be polyurethane foam, extruded polystyrene, vacuum insulation board, or aerogel composite felt.

[0038] In some embodiments, a sealing strip 14 is fixedly connected to the top of the inner liner 112 along the circumferential direction. When the lid 12 is closed on the top of the box body 11, the sealing strip 14 abuts against the inner wall of the lid 12, ensuring the sealing between the lid 12 and the box body 11.

[0039] The passive cooling module 2 includes several sealed cartridges 21, each pre-filled with phase change material. The sealed cartridges 21 are evenly arranged circumferentially inside the inner liner 112, and the sealed cartridges 21 are detachably connected to the inner wall of the inner liner 112.

[0040] In some embodiments, a sealing cartridge 21 may also be detachably connected to the inner wall of the cover 12, and the sealing cartridge 21 is pre-filled with phase change material.

[0041] Optionally, the sealing cartridge 21 is a plastic cartridge, injection molded from plastic. Specifically, the sealing cartridge 21 is divided into an upper cover and a lower box, integrally molded by precision injection molding; a filling port is pre-reserved at the edge or bottom, through which the phase change material is injected into the sealing cartridge 21, and then the filling port is sealed by heat sealing. Alternatively, the sealing cartridge 21 is a metal cartridge, formed by stamping and welding of metal. Specifically, the metal is stamped into a box shape, and after the phase change material is filled, a seamless seal is achieved by laser welding or electron beam welding to ensure that the phase change material does not leak over a long period of time.

[0042] The sealing cartridge 21 is preferably made of a metallic material, such as aluminum alloy or stainless steel. This significantly improves the thermal conductivity of the sealing cartridge 21, allowing the internal phase change material to respond quickly to temperature changes in the inner liner 112, achieving efficient heat absorption or release. This enhances the heat exchange efficiency and dynamic response capability of the entire passive system, laying a thermodynamic foundation for maintaining the temperature control window required for the cell products. More preferably, the sealing cartridge 21 made of metallic material requires surface passivation treatment to prevent reaction with the phase change material.

[0043] Regarding the selection of phase change materials (PCMs), it is necessary to strictly match the target temperature control range (such as 2~8℃ or 15~25℃), while also taking into account requirements such as biosafety, chemical stability, high latent heat value, non-corrosiveness, non-toxicity, and long-term cycle reliability. Based on this, commercially available, pharmaceutically certified low-temperature PCMs should be given priority. For example, when the temperature control range is 2~8℃, customized medical eutectic salts or hydrogel PCMs can be selected; when the temperature control range is 15~25℃, decanoic acid-lauric acid eutectic, n-hexadecane, or bio-based PCMs can be selected.

[0044] In some embodiments, the passive cooling module 2 further includes a thermally conductive and flow-limiting layer 22, which is fixedly laid on the inner walls of the inner liner 112 and the inner wall of the lid 12, and completely covers the inner walls of the inner liner 112. A connecting groove 221 is formed on the top of the thermally conductive and flow-limiting layer 22 located on the inner wall of the inner liner 112, and a connecting groove 221 is formed on one side of the thermally conductive and flow-limiting layer 22 located on the inner wall of the lid 12. The width of the connecting groove 221 is at least sufficient to accommodate a sealing cartridge 21, which is inserted into the connecting groove 221 to achieve a detachable connection between the sealing cartridge 21 and the inner wall of the inner liner 112.

[0045] This design uses the thermally conductive flow-limiting layer 22 as a controllable thermal resistance interface between the sealed cartridge 21 and the air inside the inner liner 112. This effectively regulates the heat exchange rate between the phase change material and the sample chamber, preventing a sudden drop in temperature inside the chamber due to excessively rapid release of cold energy during the initial phase change. This prevents cell products from becoming inactive due to instantaneous supercooling (e.g., below 2°C). Simultaneously, the thermally conductive flow-limiting layer 22 fully covers the sidewalls of the inner liner 112, helping to reduce the inward transmission of external thermal disturbances and improving overall insulation performance. Furthermore, the connecting groove 221 structure allows for quick insertion and removal, flexible replacement, or on-demand configuration of the sealed cartridge 21. This facilitates the selection of appropriate phase change materials for different temperature control requirements (e.g., 2~8°C or 15~25°C), and also facilitates cleaning, maintenance, and reuse, significantly improving the device's operational convenience, functional adaptability, and long-term reliability.

[0046] In some embodiments, limiting blocks 222 are provided opposite to each other on two parallel sidewalls of the connecting groove 221. For example, the limiting blocks 222 can be integrally formed with the thermally conductive and current-limiting layer 22. The limiting blocks 222 divide the connecting groove 221 into two receiving spaces along the thickness direction of the thermally conductive and current-limiting layer 22, and each receiving space can accommodate a sealing cartridge 21. One or two sealing cartridges 21 can be inserted into the same connecting groove 221. For example, when the temperature control range is 15~25°C, the sealing cartridge 21 is inserted into the inner receiving space of the same connecting groove 221; when the temperature control range is 2~8°C, both receiving spaces of the same connecting groove 221 are inserted into the sealing cartridges 21. When two sealing cartridges 21 are inserted, the temperature of the phase change material in the inner sealing cartridge 21 is lower than the temperature of the phase change material in the outer sealing cartridge 21.

[0047] This solution implements a modular and tiered passive cooling configuration. In ambient temperature storage and transportation scenarios (15~25℃), only one phase change temperature-matched sealed cartridge 21 is installed in the inner containment space near the sample chamber to avoid over-cooling; while in low temperature storage and transportation scenarios (2~8℃), sealed cartridges 21 are inserted in both the inner and outer containment spaces simultaneously. By stacking double-layer PCM, the constant temperature time is extended, the cold storage capacity is increased, and the needs of long-term cold chain transportation are met.

[0048] When the dual-sealed cartridges 21 are used simultaneously, the inner sealed cartridge 21 is filled with a PCM with a lower phase transition temperature, while the outer sealed cartridge 21 is filled with a PCM with a slightly higher phase transition temperature, forming a "low-temperature core-buffer transition" thermal gradient structure from the inside out. This design not only delays the thermal shock of the inner low-temperature PCM and prevents the sample from becoming too cold, but also provides thermal shielding for the inner PCM based on the phase transition heat absorption of the outer PCM, thus mitigating its heating rate and significantly extending the stable maintenance time of the entire system within the target temperature range.

[0049] Furthermore, the limiting block 222 and the thermally conductive flow-limiting layer 22 are integrally molded, with an indirect structure and precise positioning, ensuring a secure and good contact with the sealing cartridge 21, while avoiding the risk of thermal bridging or leakage caused by additional components. Without increasing external volume, the overall solution significantly improves the device's adaptability to the temperature control requirements of different cell products, its temperature control accuracy, and operational reliability through space reuse and functional layering, combining flexibility, efficiency, and practicality.

[0050] In some embodiments, the thermally conductive flow-limiting layer 22 is made of a low thermal conductivity material, which can be any one of closed-cell foam materials, polymer sheets, and aerogel composite materials. Closed-cell foam materials include polyethylene foam, polyurethane foam, and melamine foam, which have the advantages of low thermal conductivity and good cushioning performance, facilitating the slotted installation of the sealing cartridge 21. Polymer sheets include polypropylene, polytetrafluoroethylene, and polyetheretherketone. Aerogel composite materials include silica aerogel felt / board, suitable for cryogenic storage devices with extremely high temperature control accuracy requirements.

[0051] The active cooling module 3 includes a circulating air duct 31 and a cooler 32 and a fan 33 disposed in the circulating air duct 31. The two ends of the circulating air duct 31 are connected to the top and bottom of the inner liner 112, respectively, and the cold air blown out by the circulating air duct 31 is evenly introduced into the inner liner 112 from the top along the circumference.

[0052] Specifically, the circulating air duct 31 includes an installation cavity 311, an air distribution cavity 312, and a return cavity 313. The installation cavity 311 connects the air distribution cavity 312 and the return cavity 313. An installation cover 15 is sealed and fixedly connected to the outer wall of the outer shell 111, and the space between the installation cover 15 and the outer shell 111 forms the installation cavity 311. The interior of the cover 12 is hollow, forming the air distribution cavity 312. Several air distribution holes 314 are opened on the inner wall of the cover 12. The air distribution holes 314 are evenly arranged along the circumference of the inner liner 112 and communicate with the air distribution cavity 312 to introduce cold air from the air distribution cavity 312 into the inner liner 112. The bottom of the outer shell 111 is sealed and fixedly connected to a reflux shroud 16. The space between the reflux shroud 16 and the outer shell 111 forms a reflux cavity 313. The bottom of the box 11 has at least one reflux hole 315. The two ends of the reflux hole 315 are respectively connected to the inner liner 112 and the reflux cavity 313, and are used to introduce the cold air after heat exchange in the inner liner 112 into the reflux cavity 313.

[0053] The top of the mounting cover 15 has a connecting hole 316 that communicates with the mounting cavity 311. The inner wall of the cover 12 has an air inlet 317 that communicates with the air distribution cavity 312. The connecting hole 316 and the air inlet 317 are positioned correspondingly and communicate with each other, thus connecting the mounting cavity 311 and the air distribution cavity 312. In this embodiment, the side of the cover 12 near the mounting cover 15 extends outward and overlaps the top of the mounting cover 15 to facilitate the opening of the connecting hole 316 and the air inlet 317 at the corresponding positions, thereby achieving communication between the mounting cavity 311 and the air distribution cavity 312. The return hood 16 has an air outlet 319 on its side wall near the mounting cover 15. The mounting cover 15 covers the air outlet 319, and both ends of the air outlet 319 communicate with the mounting cavity 311 and the return cavity 313, respectively. That is, the mounting cavity 311 and the return cavity 313 are interconnected through the air outlet 319.

[0054] The active cooling module 3 in this solution, through the integrated layout of the circulating air duct 31, the cooler 32, and the fan 33, combined with the three-dimensional interconnected structure of the air distribution chamber 312, the return chamber 313, and the mounting chamber 311, achieves efficient, uniform, and stable forced convection temperature control within the insulation box 1. Its beneficial effects are specifically described below: (1) Achieve a highly uniform temperature field inside the inner liner 112. Cold air is sent into the inner liner 112 from the top through the air distribution holes 314 evenly distributed around the inner wall of the lid 12, forming a vertical airflow from top to bottom, effectively eliminating local hot or cold spots, ensuring the uniformity of the ambient temperature of the cell products, and meeting the temperature control requirements of highly sensitive biological samples.

[0055] (2) Construct a closed-loop forced circulation air duct 31 to improve cooling efficiency. Through the closed-loop airflow path of “air distribution cavity 312—inner liner 112—return cavity 313—installation cavity 311—air distribution cavity 312”, and with the drive of fan 33, the efficient circulation of cold air is achieved.

[0056] (3) High structural integration, saving internal space. The hollow structure of the box cover 12 is used as the air distribution cavity 312, the bottom of the outer shell 111 is equipped with a return hood 16 to form a return cavity 313, and the outer mounting cover 15 forms a mounting cavity 311. The air duct function is cleverly integrated into the structure of the box 11 itself, without occupying the effective volume of the inner liner 112, maximizing the sample loading space.

[0057] In some embodiments, the air distribution hole 314 is a strip-shaped hole, and the length direction of the air distribution hole 314 is parallel to the side wall direction of the corresponding inner liner 112.

[0058] The structural design of the air distribution holes 314 in this solution effectively optimizes the airflow organization performance of the active cooling module 3. Specifically, the strip-shaped air distribution holes 314 allow cold air to be uniformly delivered along the circumference of the inner liner 112 in a linear and continuous manner, significantly improving the uniformity of temperature distribution within the inner liner 112 and avoiding localized overcooling or airflow dead zones. Simultaneously, the strip-shaped air distribution holes 314 guide airflow to adhere to the inner wall, utilizing the wall-attachment effect to form a smooth, top-to-bottom circulation path, reducing direct impact on the sample container and enhancing overall heat exchange efficiency. Furthermore, compared to dot-shaped or circular holes, the strip-shaped holes have lower flow resistance for the same opening area, helping to reduce the power consumption and operating noise of the fan 33, and improving the device's quietness and energy efficiency.

[0059] In some embodiments, the bottom of the housing 11 has a plurality of reflux holes 315, and the reflux holes 315 are also strip-shaped holes, and the reflux holes 315 and the air distribution holes 314 are arranged in parallel one-to-one.

[0060] The design of the reflux vent 315 creates a vertically aligned and directional airflow channel layout, significantly improving the air circulation efficiency and temperature control performance of the active cooling module 3. This structure allows the cold air delivered from the top air distribution vent 314 to flow smoothly down the side wall of the inner liner 112 after cooling the sample area, and then efficiently return to the reflux chamber 313 through the matching bottom strip-shaped reflux vent 315, forming a closed-loop airflow circuit with a clear path and less resistance. This symmetrical and aligned air inlet and outlet design effectively avoids airflow turbulence, eddies, or stagnant zones, enhances the orderliness of airflow and heat exchange uniformity within the chamber, and further strengthens the wall-adhering flow effect, reducing disturbance to the cell product container.

[0061] In some embodiments, a plurality of air guide plates 121 are fixedly connected to the inner wall of the cover 12. The air guide plates 121 are arranged one-to-one on the side of the air distribution hole 314 away from the inner liner 112, and the bottom end of the air guide plate 121 is biased towards the inner liner 112 to guide the cold air blown out of the air distribution hole 314 to the inner wall of the inner liner 112.

[0062] This design incorporates an air guide plate 121, which effectively guides and directs the cold air blowing out of the air distribution holes 314. This structure ensures that the cold airflow is immediately constrained and deflected by the air guide plate 121 after leaving the air distribution holes 314, precisely adhering to the inner wall of the inner liner 112 and flowing downwards. This enhances the wall-attachment effect and prevents direct airflow from blowing directly onto the sample container, thus avoiding localized overcooling or disturbance. Simultaneously, this directional airflow method helps to form a stable and uniform vertical circulating airflow, improving the temperature uniformity and heat exchange efficiency of the entire inner liner 112 space.

[0063] In some embodiments, the inner wall of the cover 12 is sealed with an air intake channel 318, which surrounds the air intake hole 317. When the cover 12 is closed with the body 11, the air intake channel 318 extends into the mounting cavity 311 through the connecting hole 316, and the outer wall of the air intake channel 318 slides in fit with the inner wall of the connecting hole 316.

[0064] This solution adds an air intake channel 318, which can significantly improve the sealing performance of the active cooling module 3. Specifically, the air intake channel 318 physically surrounds and seals the air intake hole 317, effectively preventing cold air from leaking at the joint between the cover 12 and the body 11, ensuring the airtightness of the circulating air duct 31, thereby maintaining stable cooling efficiency and temperature uniformity.

[0065] In some embodiments, an annular partition plate 122 is provided circumferentially around the inner liner 112 inside the air distribution cavity 312. The upper and lower ends of the partition plate 122 are sealed and fixedly connected to the top and bottom of the air distribution cavity 312, respectively, for example, by welding. The partition plate 122 divides the air distribution cavity 312 into an annular shape, and the space inside the partition plate 122 forms an isolation cavity 123. A top insulation layer 124 is installed inside the isolation cavity 123. Specifically, the material of the top insulation layer 124 can be polyurethane foam, extruded polystyrene, vacuum insulation board, or aerogel composite felt.

[0066] This design incorporates an isolation panel 122 and a top insulation layer 124, effectively optimizing functional zoning and thermal management. On one hand, the isolation panel 122 physically separates the air distribution chamber 312 used for air circulation from the non-functional space at the top of the enclosure 11, preventing ineffective diffusion or stagnation of cold air at the top and ensuring concentrated and efficient airflow through the air distribution holes 314 into the inner liner 112, improving cooling efficiency and temperature uniformity. On the other hand, the top insulation layer 124, installed within the isolation chamber 123, significantly enhances the thermal insulation performance of the cover 12 area, blocking external heat transfer to the inner liner 112 through the cover 12, effectively reducing cold loss, especially in high-temperature environments or during long-term transportation. Simultaneously, this design completely encloses the insulation structure within the isolation chamber 123, preventing the insulation material from being exposed to the airflow channels, thus avoiding the risk of particle shedding or contamination, balancing thermal performance and biocompatibility requirements. Overall, this solution achieves the dual goals of "airflow guidance" and "top insulation" through structural integration, further improving the temperature control stability, energy efficiency, and compliance safety of the cryogenic storage device.

[0067] The cooler 32 and the fan 33 are disposed in the mounting cavity 311. Specifically, the cooler 32 is fixedly connected to the side wall of the mounting cover 15, and the fan 33 is horizontally disposed. The opposite sides of the fan 33 are fixedly connected to the outer casing 111 and the mounting cover 15, respectively.

[0068] The cooler 32 is a semiconductor cooler 321, which is embedded in the side wall of the mounting cover 15. Specifically, the side wall of the mounting cover 15 has a mounting hole, the two ends of which connect the mounting cavity 311 to the external environment. A mounting sleeve 34 is fixedly inserted into the mounting hole, and the semiconductor cooler 321 is fixedly inserted into the mounting sleeve 34. A cold end heat sink 322 is fixedly connected to the cold end of the semiconductor cooler 321. The cold end heat sink 322 is located inside the mounting cover 15 and is used for heat exchange with the air in the mounting cavity 311. A hot end heat sink 323 is fixedly connected to the hot end of the semiconductor cooler 321. The hot end heat sink 323 is located outside the mounting cover 15 and is used for heat exchange with the external environment.

[0069] This design effectively isolates the heat exchange path between the hot and cold ends. The cold-end heat sink 322 is placed inside the mounting cavity 311, efficiently exchanging heat with the air in the circulating air duct 31 to continuously provide cooling to the inner liner 112. The hot-end heat sink 323 is exposed outside the cabinet 11, directly dissipating heat to the environment and preventing heat from flowing back into the cabinet, significantly improving cooling efficiency and energy efficiency ratio. The mounting sleeve 34 not only facilitates the precise positioning and quick replacement of the semiconductor cooler 321 but also enhances sealing and structural stability. The fan 33 is horizontally fixed between the outer shell 111 and the mounting cover 15, with the airflow direction matching the mounting cavity 311, enhancing the convective heat transfer of the cold-end heat sink 322 and promoting uniform airflow within the mounting cavity 311 to prevent localized overheating. The overall layout makes full use of the space surrounding the cabinet 11 without occupying the volume of the inner liner 112, balancing cooling performance, ease of maintenance, and system reliability.

[0070] Specifically, the cooler 32 employs a semiconductor cooler 321, capable of switching current direction to achieve cooling or heating functions according to actual temperature control requirements. When the temperature of the inner liner 112 exceeds a set threshold, the system activates the cooling mode for active cooling; when the temperature of the inner liner 112 falls below the set threshold, it switches to the heating mode for active heating. This bidirectional temperature control capability allows the device to be suitable not only for low-temperature transportation (e.g., 2~8℃) but also for precisely maintaining a normal temperature range (e.g., 15~25℃), effectively covering the differentiated temperature control requirements of various cell products and significantly improving the adaptability, control accuracy, and intelligence level of the equipment.

[0071] In some embodiments, a sealant is provided between the mounting sleeve 34 and the mounting hole. The sealant can seal any gaps that may exist between the mounting sleeve 34 and the mounting hole, thereby preventing cold air in the mounting cavity 311 from escaping from the mounting hole and reducing unnecessary energy consumption.

[0072] In some embodiments, the end of the mounting sleeve 34 near the hot-end heat sink 323 is flush with the outer wall of the mounting cover 15, and the end of the mounting sleeve 34 near the cold-end heat sink 322 extends into the mounting cavity 311. The hot-end heat sink 323 is located inside the mounting sleeve 34, and the end of the hot-end heat sink 323 away from the thermoelectric cooler 321 is flush with the outer wall of the mounting cover 15. In this way, the entire structure of the cooler 32 is located inside the mounting cover 15, avoiding damage from impacts.

[0073] In some embodiments, the sidewalls of the mounting cover 15 are hollow, and the hollow sidewalls of the mounting cover 15 are filled with insulation material. Specifically, the insulation material can be polyurethane foam, extruded polystyrene, vacuum insulation board, or aerogel composite felt. This reduces heat exchange between the external environment and the air inside the mounting cavity 311 through the mounting cover 15, ensuring that the air temperature delivered to the inner liner 112 by the active cooling module 3 meets requirements, while also reducing the need for compensating for heat exchange with the external environment by the active cooling module 3, thereby reducing energy consumption.

[0074] In some embodiments, the mounting sleeve 34 is made of a low thermal conductivity material, such as engineering plastic POM or bakelite. This reduces heat exchange between the external environment and the air inside the mounting cavity 311 through the mounting sleeve 34.

[0075] The control module 4 includes a controller 41 and a temperature sensor 42, with the temperature sensor 42 housed within the inner liner 112. Specifically, the controller 41 is fixedly connected to the side wall of the mounting cover 15. The temperature sensor 42, the thermoelectric cooler 321, and the fan 33 are all electrically connected to the controller 41. The controller 41 is a PID controller, and the thermoelectric cooler 321 and the controller 41 are connected via an H-bridge circuit. The temperature sensor 42 can be a PT1000 platinum resistance temperature sensor, a high-precision NTC thermistor, or a digital temperature sensor (such as a Si7051).

[0076] In some embodiments, the control module 4 further includes a mounting bracket 43 disposed within the inner liner 112. The mounting bracket 43 includes a telescopic rod 431 and a flexible connecting rod 432 with a fixed posture. The flexible connecting rod 432 can employ existing technologies such as a universal snake-bone tube or a flexible gooseneck tube; its structure will not be described in detail in this embodiment. The bottom end of the telescopic rod 431 is detachably connected to the inner bottom of the inner liner 112 via bolts or other connecting components. The top end of the telescopic rod 431 is fixedly connected to one end of the flexible connecting rod 432, and the other end (free end) of the flexible connecting rod 432 is fixedly connected to the temperature sensor 42. The telescopic rod 431 is used to adjust the height of the temperature sensor 42, and the flexible connecting rod 432 is used to adjust the spatial position of the temperature sensor 42, bringing the temperature sensor 42 closer to the cell product.

[0077] This design incorporates a mounting bracket 43, which, through the cooperation of a telescopic rod 431 and a flexible connecting rod 432, enables flexible and precise adjustment of the temperature sensor 42's position. Specifically, the telescopic rod 431 adjusts the sensor's vertical position according to the sample loading height; the flexible connecting rod 432 can be bent arbitrarily in three-dimensional space while maintaining a fixed posture, allowing the temperature sensor 42 to reliably fit close to the actual cell product container, accurately reflecting its microenvironment temperature. This structure avoids the temperature measurement lag or deviation caused by traditional fixed sensors being far from the sample, significantly improving the accuracy and representativeness of temperature monitoring. This provides a more reliable feedback basis for the temperature control system, ensuring the safety and activity of the cell products during transportation.

[0078] In some embodiments, the control module 4 further includes a switch button 44, a temperature adjustment button 45, and a display screen 46 disposed on the outer wall of the insulation box 1. For example, the switch button 44, the temperature adjustment button 45, and the display screen 46 can be fixedly connected to the outer wall of the mounting cover 15. The switch button 44, the temperature adjustment button 45, and the display screen 46 are all electrically connected to the controller 41. The switch button 44 is used to turn the active cooling module 3 and the control module 4 on and off, the temperature adjustment button 45 is used to set the storage temperature, and the display screen 46 is used to display the set temperature and / or the actual temperature detected by the temperature sensor 42.

[0079] This solution adds a switch button 44, a temperature adjustment button 45, and a display screen 46 to the outer wall of the insulated box 1, enabling intuitive and convenient operation and real-time monitoring of the active cooling and temperature control system. Users can start and stop the equipment with one button and flexibly set the target transport temperature without opening the box 11. They can also view the set value and the actual temperature fed back by the temperature sensor 42 in real time through the display screen 46, significantly improving the efficiency of human-machine interaction and ease of use.

[0080] In some embodiments, when the operating mode of the thermoelectric cooler 321 is switched, such as from cooling to heating, the controller 41 needs to introduce a dead time, pausing for a few seconds before reversing the power supply. In this way, the large temperature difference between the cold and hot ends of the thermoelectric cooler 321 can be allowed to dissipate first, avoiding damage to the ceramic substrate of the thermoelectric cooler 321 caused by the instantaneous large temperature difference generating huge thermal stress.

[0081] The cryogenic storage device also includes a liquid collection module 5, which includes a liquid collection drawer 51. The side wall of the reflux hood 16 has a pull-out opening 52 communicating with the reflux chamber 313, and the liquid collection drawer 51 is slidably inserted into the pull-out opening 52. For example, the width of the pull-out opening 52 is the same as the width of the reflux chamber 313. Slide rails are installed between the two side walls of the liquid collection drawer 51 and the corresponding side walls of the reflux chamber 313. The liquid collection drawer 51 is slidably connected to the side wall of the mounting cavity 311 via the slide rails, thereby enabling the liquid collection drawer 51 to slide in and out of the pull-out opening 52. If the horizontal projection of the reflux hole 315 is within the range of the liquid collection drawer 51, then the condensate in the inner liner 112 can drip through the reflux hole 315 into the liquid collection drawer 51 for collection and subsequent centralized processing.

[0082] In some embodiments, a sealing element is provided between the liquid collection drawer 51 and the pull-out opening 52. Specifically, the sealing element may be selected from the following structures: (1) Lip seal: installed in the groove opened in the inner wall of the pull-out opening 52, with the lip facing the sliding direction of the liquid collection drawer 51; after the liquid collection drawer 51 is pushed in, the lip is pressed tightly against the surface of the liquid collection drawer 51 to form a dynamic seal. (2) O-ring mating groove structure: a rectangular or trapezoidal sealing groove is processed around the pull-out opening 52, and the O-ring is embedded in it; the corresponding position of the liquid collection drawer 51 is designed as a smooth and flat sealing surface, and the O-ring is compressed after insertion to achieve a seal. The setting of the sealing element can improve the sealing performance at the pull-out opening 52 and reduce the heat exchange between the inside of the heat preservation box 1 and the external environment.

[0083] In some embodiments, the liquid collection module 5 further includes a guide plate 53, which is circumferentially disposed in the inner liner 112. The guide plate 53 is connected to the side wall of the inner liner 112 with a gap via connecting bolts 54, such that the air distribution hole 314 and the corresponding return hole 315 are located between the guide plate 53 and the side wall of the inner liner 112. The top of the guide plate 53 bends inward, forming a larger space at the connection between the air distribution hole 314 and the inner liner 112. This is also the location where cold air in the circulating air duct 31 enters the inner liner 112, where condensation is most likely to form. The inward bending of the top of the guide plate 53 can effectively guide the condensation, allowing it to flow downward along the guide plate 53. The gap between the bottom of the baffle 53 and the bottom of the inner liner 112 not only promotes heat exchange between the air inside and outside the baffle 53, but also facilitates the condensate formed inside the baffle 53 to flow along the bottom of the inner liner 112 to the outside of the baffle 53, and then drips into the liquid collection drawer 51 below through the return hole 315.

[0084] In some embodiments, the baffle plate 53 has at least one heat exchange hole 55. For example, the heat exchange hole 55 can be a plurality of circular holes evenly arrayed on the baffle plate 53, or a plurality of strip holes arranged side by side. The arrangement of the heat exchange hole 55 can further promote heat exchange between the air inside and outside the baffle plate 53, ensuring that the cold air introduced from the circulating air duct 31 effectively cools the inner liner 112.

[0085] In some embodiments, the bottom of the mounting cover 15 is inclined, with the side closer to the return shroud 16 being lower and the side farther from the return shroud 16 being higher, and the side closer to the return shroud 16 being substantially flush with the bottom of the air outlet 319.

[0086] During the operation of the active cooling module 3 in cooling mode, because the semiconductor cooler 321 is mounted on the mounting cover 15 and its cold end is located inside the mounting cavity 311, the temperature inside the mounting cavity 311 is relatively low throughout the entire circulating air duct 31. After heat exchange in the inner liner 112, the gas returns to the mounting cavity 311 via the return hole 315, the return cover 16, and the outlet 319. Upon encountering cold air, it easily condenses into water on the cavity wall or the surface of the device. If this condensate remains inside the mounting cavity 311, it may not only corrode electronic components and reduce system reliability, but the accumulated moisture in the mounting cavity 311 may also enter the inner liner 112 with the airflow, causing damage to the container label or even contaminating the cell products.

[0087] This design tilts the bottom of the mounting cover 15, allowing condensate to flow naturally under gravity to the lowest vent 319 area. From there, the condensate drains into the return chamber 313 and finally collects in the collection drawer 51 for centralized collection and treatment. This structure eliminates the need for additional drain pumps or complex piping, achieving passive, self-draining condensate management. This ensures the safe operation of electrical components and avoids potential interference from moisture on the cell product environment.

[0088] The cryopreservation device also includes a sample support frame 6, which comprises a tray 61 and a support member 62. The tray 61 is used to place containers containing cell products. The support member 62 is fixedly connected between the bottom of the tray 61 and the bottom of the inner liner 112, and is used to support the tray 61 from the bottom of the inner liner 112, thereby supporting the containers containing cell products from the bottom of the inner liner 112. This prevents condensation that may exist at the bottom of the inner liner 112 from wetting and damaging the labels on the containers, and ensures the readability of the information recorded on the labels.

[0089] In some embodiments, a collection trough 611 is circumferentially formed on the inner bottom side of the tray 61, and a dropper 612 is sealed and fixedly connected (e.g., welded or integrally formed) to the outer bottom side of the tray 61, communicating with the collection trough 611. During cryogenic transport, the container of the cell product may be damaged due to collision or other reasons, causing the cell product to leak into the tray 61. In this case, the leaked solution in the collection trough 611 is collected and discharged into the inner liner 112 through the dropper 612, and finally drips into the collection drawer 51 through the return hole 315. In this way, the leaked solution can be prevented from remaining in the tray 61 for a long time, wetting and damaging the labels on other containers, or even contaminating the cell products in other containers, which helps to ensure the readability of the labels and the validity of the intact cell products.

[0090] In some embodiments, there are multiple support members 62, and these multiple support members 62 are evenly arranged along the circumference of the tray 61. Specifically, a connecting plate is fixedly connected to the bottom of the tray 61 at the position corresponding to the support member 62. A connecting hole is opened on the connecting plate at the position corresponding to the support member 62. A connecting post is fixedly connected to the top of the support member 62, and the connecting post is vertically inserted into the connecting hole. The fixed connection between the support member 62 and the tray 61 is achieved through the fixed connection between the connecting post and the connecting plate. In this way, the structural stability of the sample support frame 6 can be improved, ensuring stable support for cell products and reducing damage to cell products during transportation.

[0091] In some embodiments, the support member 62 adopts a solid or hollow one-piece columnar structure. In this case, the relative position of the tray 61 and the inner liner 112 is fixed, which can ensure that the tray 61 stably supports the cell products.

[0092] Alternatively, in some embodiments, the support 62 may be a shock absorber. In this case, the relative position of the tray 61 and the inner liner 112 is not fixed, and the shock absorber can reduce vibration during transport, ensuring the effectiveness of the cell products. Specifically, the shock absorber may be a spring-damping composite shock absorber, a medical silicone shock-absorbing pad, or an integrated flexible support foot. All of the above shock absorber types are prior art, and their structures will not be described in detail here.

[0093] In use, the phase change material pre-filled in the sealed cartridge 21 is used for passive cooling through phase change heat absorption; the temperature sensor 42 is used to detect the temperature inside the inner liner 112 and send a temperature detection signal to the controller 41; when the temperature detection signal shows that the temperature inside the inner liner 112 is higher than the threshold, the controller 41 controls the start of the semiconductor cooler 321 and the fan 33 according to the temperature detection signal, and performs active cooling simultaneously; when the temperature detection signal shows that the temperature inside the inner liner 112 is lower than the threshold, the controller 41 controls the start of the semiconductor cooler 321 and the fan 33 according to the temperature detection signal, and performs active heating simultaneously. The specific steps are as follows: S1. Pre-treatment stage: According to the target transportation temperature control range (such as 2~8℃ or 15~25℃), select the corresponding sealing cassette 21 with the phase change temperature, and place it in a low temperature or constant temperature environment for pre-cooling or preheating, so that the internal phase change material can fully complete the phase change energy storage; at the same time, place the container containing the cell products on the sample support rack 6 in the inner liner 112 according to the specifications.

[0094] S2. Install the temperature control components: Insert the pre-treated sealed cartridge 21 into the connecting groove 221 of the thermally conductive and flow-limiting layer 22, and select a single-layer or double-layer configuration according to the temperature control requirements; adjust the telescopic rod 431 and flexible connecting rod 432 on the mounting bracket 43 to make the temperature sensor 42 close to the cell product container to ensure accurate temperature measurement.

[0095] S3. Set operating parameters: Close the cover 12 and confirm that the box body 11 and the cover 12 are sealed in place; set the target transport temperature (such as 4℃ or 22℃) through the temperature adjustment button 45 on the outer wall of the mounting cover 15, and check the set value through the display screen 46; press the switch button 44 to start the control module 4 and the active cooling module 3.

[0096] S4. Monitoring During Storage: After the device enters the temperature control operation state, the passive cooling module 2, as the main temperature maintenance unit, relies on the pre-charged phase change material to continuously and stably maintain the temperature of the inner liner 112 within the target range through endothermic or exothermic phase change processes. The temperature sensor 42 monitors the temperature of the inner liner 112 in real time and transmits the temperature detection signal to the controller 41. When the passive cooling capacity fluctuates due to environmental disturbances (such as high temperature exposure or frequent opening of the lid), and the temperature of the inner liner 112 deviates from the set threshold, the controller 41 activates the active cooling module 3 for auxiliary adjustment: if the temperature is higher than the upper threshold, the semiconductor cooler 321 is driven into cooling mode and the fan 33 is turned on to accelerate cooling; if the temperature is lower than the lower threshold, the semiconductor cooler 321 is switched to heating mode, and the fan 33 is used to achieve uniform heating. The active cooling module 3 intervenes only when necessary, playing a role in precise fine-tuning and dynamic compensation, which not only ensures the stability of temperature control but also significantly reduces energy consumption and extends the effective operating time of the system under conditions without external power.

[0097] S5. Unpacking Operation: After the low-temperature preservation of static storage or dynamic transportation is completed, check the current actual temperature through the display screen 46; turn off the switch button 44, open the box cover 12, and take out the cell products; the detachable sealed cartridge 21, sample holder 6 and temperature sensor 42 are easy to clean, sterilize or replace, and are ready for the next use.

[0098] It is worth noting that, in addition to cell products, human tissues, organs, animal and plant bodies, or parts thereof (such as skin, cornea, embryos, seeds, and live samples) often require short-term or medium- to long-term preservation and transportation in an in vitro state during scientific research, clinical practice, or seed preservation. These biological materials are also highly sensitive to the temperature of the preservation environment, and their activity, structural integrity, and even genetic stability are easily damaged by improper temperature control. Therefore, the environmental control capabilities of cryopreservation devices are subject to requirements similar to those for cell products.

[0099] Although the existing technology (the invention patent with publication number CN111543423A) discloses an intelligent portable multifunctional cell or tissue cryopreservation and transport device, which can achieve cryopreservation and transport of human, animal and plant bodies or their parts to a certain extent, there are still technical problems in practical applications, such as uneven temperature distribution inside the chamber, local overcooling or overheating, large temperature fluctuations, and difficulty in maintaining the target temperature control window stably. It is difficult to meet the stringent requirements of highly sensitive live biological samples for a precise, stable, and uniform temperature control environment.

[0100] This solution is used for the cryopreservation of human, animal, or plant organisms, or parts thereof (including but not limited to cell products, tissues, organs, embryos, seeds, and live samples) during static storage or dynamic transport. Through coordinated active and passive cooling, 360° three-dimensional air circulation, near-sample temperature monitoring, and precise feedback control, it significantly improves the uniformity and stability of the temperature field within the chamber, effectively suppressing localized overcooling or overheating and strictly controlling temperature fluctuations within ±0.5℃. Furthermore, combined with gradient phase change materials and an intelligent bidirectional temperature control strategy, it can flexibly adapt to various temperature control ranges, such as 2~8℃ and 15~25℃. Therefore, this solution not only ensures the activity, structural integrity, and functional stability of biological samples throughout the entire transport process but also meets the core requirements for the precise, reliable, and compliant preservation of highly sensitive live materials in clinical, research, and seed preservation scenarios.

[0101] Example 2: This embodiment provides a cell product cryopreservation device, which differs from Embodiment 1 in that: the control module 4 includes multiple temperature sensors 42, which are arranged circumferentially along the tray 61 via a mounting bracket 43 to detect the temperature of cell products at different locations or at different locations of the same cell product.

[0102] Compared to the single-point detection in Example 1, this solution can comprehensively reflect the temperature field distribution within the inner liner 112, promptly detecting risks such as localized overheating, overcooling, or abnormal temperature gradients, thus avoiding sample damage caused by temperature measurement blind spots. Simultaneously, multi-point temperature data provides more reliable feedback to the controller 41, enabling it to be more targeted and balanced when initiating active cooling or heating regulation, further improving overall temperature control uniformity and stability. Furthermore, this design is particularly suitable for scenarios involving multiple samples or large containers, effectively ensuring the safety and activity consistency of various cell products throughout the entire preservation and transportation process.

[0103] Example 3: This embodiment provides a cell product cryopreservation device, which differs from Embodiment 1 or 2 in that: a temperature sensor 42 is also provided on the inner wall of the inner liner 112, and the temperature sensor 42 is used to detect the ambient temperature in the inner liner 112.

[0104] This solution adds a temperature sensor 42 to the inner wall of the inner chamber 112 to detect the overall ambient temperature of the inner chamber 112, thus constructing a multi-dimensional, multi-layered temperature sensing system. This design not only accurately captures local temperature changes near the sample using existing sensors, but also uses the sensor on the inner wall of the inner chamber to monitor the overall temperature field of the air inside the chamber in real time, effectively distinguishing between ambient temperature fluctuations and anomalies caused by local heat / cold sources, improving the accuracy and robustness of temperature control judgments. Simultaneously, when uneven sample loading, changes in sample quantity, or container obstruction cause localized temperature measurement limitations, the temperature sensor 42 on the inner wall of the inner chamber 112 serves as a reliable redundant backup, ensuring the system always has effective temperature feedback. This dual monitoring mechanism of "microenvironment + macroenvironment" significantly enhances the adaptability of the cryopreservation device to complex preservation and transportation conditions, providing more comprehensive and reliable temperature control protection for cell products.

[0105] Example 4: This embodiment provides a cell product cryopreservation device, which differs from Embodiment 1 in that: a latch is provided on the non-hinged side of the lid 12 at the corresponding position of the outer shell 111.

[0106] For example, a latch is installed on the side of the lid 12 opposite to the hinge position and the corresponding position of the outer shell 111. After the lid 12 is closed on the top of the box body 11, the latch can fix the side of the lid 12 opposite to the hinge position to the corresponding position of the outer shell 111, preventing the lid 12 from opening during transportation and ensuring the temperature inside the inner liner 112 is stable.

[0107] Example 5: This embodiment provides a cell product cryopreservation device, which differs from Embodiment 3 in that: the top cover of the box 12 is provided with a handle, which makes it easy to lift the entire insulated box 1 for relocation.

[0108] In other embodiments, the handle can be hinged to the lid 12 so that after the insulated box 1 is placed stably, the handle can be rotated to fit against the lid 12, reducing space occupation.

[0109] Example 6: This embodiment provides a cryogenic preservation device for cell products, which differs from Embodiment 1 in that: an adhesive area is provided at the joint between the lid 12 and the body 11, for attaching a disposable tamper-evident seal. The disposable tamper-evident seal uses fragile materials or VOID anti-counterfeiting technology to indicate whether the insulated box 1 has been illegally opened during storage and transportation.

[0110] This solution effectively prevents cell products from being swapped, contaminated, or tampered with during cryogenic storage and cold chain transportation, ensuring the integrity, authenticity, and biosafety of samples. It is particularly suitable for clinical-grade cell therapy products, high-value biological samples, or scenarios that require compliance with GMP / GLP requirements.

[0111] It should be noted that, in this document, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Unless otherwise specified, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes that element.

[0112] The embodiments of the present invention have been described above with reference to the accompanying drawings. However, the present invention is not limited to the specific embodiments described above. The specific embodiments described above are merely illustrative and not restrictive. Those skilled in the art can make many other forms under the guidance of the present invention without departing from the spirit and scope of the claims. All of these forms are within the protection scope of the present invention.

Claims

1. A cryopreservation device for cell products, characterized in that, The system includes an insulated box (1) and a passive refrigeration module (2), an active refrigeration module (3), and a control module (4) disposed within the insulated box (1). The insulated box (1) includes a box body (11) and a lid (12) that can be opened and closed on the top of the box body (11). The box body (11) includes an outer shell (111) and an inner liner (112) connected to the outer shell (111). A side insulation layer (113) is provided circumferentially between the outer shell (111) and the inner liner (112). The passive refrigeration module (2) includes several sealing cartridges (21). The sealing cartridges (21) are pre-filled with phase change material. The several sealing cartridges (21) are evenly disposed circumferentially within the inner liner (112), and the sealing cartridges (21) are detachably connected to the inner wall of the inner liner (112). The active refrigeration module (4) 3) Includes a circulating air duct (31) and a cooler (32) and a fan (33) disposed in the circulating air duct (31). The two ends of the circulating air duct (31) are connected to the top and bottom of the inner liner (112) respectively, and the cold air blown out by the circulating air duct (31) is evenly introduced into the inner liner (112) from the top along the circumference. The control module (4) includes a controller (41) and a temperature sensor (42). The temperature sensor (42) is located in the inner liner (112). The temperature sensor (42), the cooler (32) and the fan (33) are all electrically connected to the controller (41). The passive cooling module (2) also includes a thermally conductive flow-limiting layer (22). The thermally conductive flow-limiting layer (22) is laid on the inner side wall of the inner liner (112). The active cooling module (3) is a TEC. The circulating air duct (31) includes a mounting cavity (311), a distribution cavity (312), and a return cavity (313). The mounting cavity (311) connects the distribution cavity (312) and the return cavity (313). A mounting cover (15) is sealed to the outer wall of the outer casing (111). The space between the mounting cover (15) and the outer casing (111) forms the mounting cavity (311). The cooler (32) and the fan (313) are connected to the mounting cavity (311). 3) Set inside the mounting cavity (311); the interior of the box cover (12) is hollow to form an air distribution cavity (312), and a plurality of air distribution holes (314) are evenly opened on the inner wall of the box cover (12) along the circumference of the inner liner (112). The air distribution holes (314) communicate with the air distribution cavity (312) to allow the cold air in the air distribution cavity (312) to enter the inner liner (112); the bottom of the outer shell (111) is sealed with a A reflux hood (16) is provided, and the space between the reflux hood (16) and the outer shell (111) forms a reflux cavity (313). At least one reflux hole (315) is provided at the bottom of the box (11). The two ends of the reflux hole (315) are respectively connected to the inner liner (112) and the reflux cavity (313) to allow the cold air after heat exchange in the inner liner (112) to enter the reflux cavity (313). The phase change material pre-filled in the sealing cartridge (21) is used for passive cooling through phase change heat absorption. The temperature sensor (42) is used to detect the temperature inside the inner liner (112) and send a temperature detection signal to the controller (41). When the temperature detection signal shows that the temperature inside the inner liner (112) is higher than the threshold, the controller (41) is used to control the refrigerator (32) and the fan (33) to start according to the temperature detection signal, and at the same time perform active cooling. The cooler (32) is a semiconductor cooler (321), which is embedded in the side wall of the mounting cover (15). The cold end of the semiconductor cooler (321) is provided with a cold end heat sink (322), which is located inside the mounting cover (15) and is used to exchange heat with the air in the mounting cavity (311). The hot end of the semiconductor cooler (321) is provided with a hot end heat sink (323), which is located outside the mounting cover (15) and is used to exchange heat with the external environment. The target temperature control range of the cell product cryopreservation device is 2~8 ℃ or 15~25 ℃.

2. The cell product cryopreservation device according to claim 1, characterized in that, The air distribution hole (314) is a strip-shaped hole, and the length direction of the air distribution hole (314) is parallel to the direction of the corresponding inner liner (112) side wall; And / or, the bottom of the housing (11) is provided with a plurality of the reflux holes (315), and the reflux holes (315) are strip-shaped holes, and the reflux holes (315) and the air distribution holes (314) are arranged in parallel one to one; And / or, a plurality of air guide plates (121) are provided on the inner wall of the box cover (12). The air guide plates (121) are arranged one-to-one on the side of the air distribution hole (314) away from the inner liner (112), and the bottom end of the air guide plate (121) is biased toward the corresponding side wall of the inner liner (112) to guide the cold air blown out by the air distribution hole (314) to the inner side wall of the inner liner (112).

3. The cell product cryopreservation device according to claim 2, characterized in that, The top of the mounting cover (15) is provided with a communication hole (316) that communicates with the mounting cavity (311), and the inner wall of the box cover (12) is provided with an air inlet (317) that communicates with the air distribution cavity (312). The communication hole (316) and the air inlet (317) are opposite to each other and communicate with each other, and are used to connect the mounting cavity (311) and the air distribution cavity (312). And / or, the inner wall of the cover (12) is sealed with an air intake channel (318), the air intake channel (318) surrounds the air intake hole (317), the air intake channel (318) extends into the mounting cavity (311) through the connecting hole (316), and the outer wall of the air intake channel (318) slides with the inner wall of the connecting hole (316); And / or, the reflux shroud (16) has an air outlet (319) on the side wall near the mounting shroud (15), the mounting shroud (15) covers the air outlet (319) therein, and the two ends of the air outlet (319) are respectively connected to the mounting cavity (311) and the reflux cavity (313).

4. A cell product cryopreservation device according to claim 2, characterized in that, An annular partition plate (122) is provided inside the air distribution cavity (312) along the circumference of the inner liner (112). The two ends of the partition plate (122) are respectively sealed to the top and bottom of the air distribution cavity (312), dividing the air distribution cavity (312) into an annular shape. The space inside the partition plate (122) forms an isolation cavity (123), and a top insulation layer (124) is provided inside the isolation cavity (123). And / or, the sealing cartridge (21) is detachably connected to the inner wall of the box cover (12), and the sealing cartridge (21) is pre-filled with phase change material.

5. The cell product cryopreservation device according to claim 1, characterized in that, The top of the thermally conductive flow-limiting layer (22) is provided with a connecting groove (221), the width of which is at least able to accommodate one of the sealing cartridges (21), and the sealing cartridges (21) are inserted into the connecting groove (221); And / or, limiting blocks (222) are provided opposite to each other on the parallel side walls of the connecting groove (221). The limiting blocks (222) divide the connecting groove (221) into two receiving spaces along the thickness direction of the thermally conductive flow-limiting layer (22). Each receiving space can accommodate one sealing cartridge (21). One or two sealing cartridges (21) can be inserted into the same connecting groove (221). When two sealing cartridges (21) are inserted, the temperature of the phase change material in the inner sealing cartridge (21) is lower than the temperature of the phase change material in the outer sealing cartridge (21).

6. A cell product cryopreservation device according to claim 2, characterized in that, When the temperature detection signal indicates that the temperature inside the inner liner (112) is lower than the threshold, the controller (41) controls the semiconductor cooler (321) and the fan (33) to start according to the temperature detection signal, and performs active heating.

7. A cell product cryopreservation device according to claim 2, characterized in that, It also includes a liquid collection module (5), which includes a liquid collection drawer (51); the side wall of the reflux hood (16) is provided with a pull-out opening (52) that communicates with the reflux chamber (313), the liquid collection drawer (51) is slidably inserted into the pull-out opening (52), and the horizontal projection of the reflux hole (315) is located within the range of the liquid collection drawer (51); And / or, a seal is provided between the liquid collection drawer (51) and the pull-out opening (52); And / or, the liquid collection module (5) further includes a guide plate (53), which is connected to the side wall of the inner liner (112) with a gap by a connecting bolt (54), and the air distribution hole (314) and the return hole (315) are located between the guide plate (53) and the side wall of the inner liner (112); the top of the guide plate (53) is bent inward, and a gap is left between the bottom of the guide plate (53) and the bottom of the inner liner (112); And / or, at least one heat exchange hole (55) is provided on the guide plate (53).

8. A cell product cryopreservation device according to claim 1, characterized in that, It also includes a sample holder (6), which includes a tray (61) and a support (62); the support (62) is connected between the bottom of the tray (61) and the bottom of the inner liner (112), the tray (61) being used to place a container containing the cell product; And / or, a liquid collection groove (611) is provided on the inner bottom side of the tray (61) along the circumferential direction, and a dropper (612) is provided on the outer bottom side of the tray (61), and the dropper (612) is connected to the liquid collection groove (611). And / or, the support (62) is a shock absorber.

9. A cell product cryopreservation device according to claim 1, characterized in that, The control module (4) further includes a mounting bracket (43) disposed inside the inner liner (112). The mounting bracket (43) includes a telescopic rod (431) and a flexible connecting rod (432) with a fixed posture. The bottom end of the telescopic rod (431) is detachably connected to the inner liner (112), and the top end of the telescopic rod (431) is fixedly connected to the flexible connecting rod (432). The temperature sensor (42) is connected to the free end of the flexible connecting rod (432). The telescopic rod (431) is used to adjust the height of the temperature sensor (42), and the flexible connecting rod (432) is used to adjust the spatial position of the temperature sensor (42) so that the temperature sensor (42) is close to the cell product. And / or, the control module (4) further includes a switch button (44), a temperature adjustment button (45) and a display screen (46) disposed on the outer wall of the insulation box (1). The switch button (44), the temperature adjustment button (45) and the display screen (46) are all electrically connected to the controller (41). The switch button (44) is used to turn the active cooling module (3) and the control module (4) on and off. The temperature adjustment button (45) is used to set the storage temperature. The display screen (46) is used to display the set temperature and / or the actual temperature detected by the temperature sensor (42).

10. A cell product cryopreservation device according to claim 1, characterized in that, One side of the lid (12) is hinged to the corresponding position of the outer shell (111) via a hinge (13), and a latch is provided on the non-hinged side of the lid (12) and the corresponding position of the outer shell (111); And / or, the top of the lid (12) is provided with a handle; And / or, the top of the inner liner (112) is provided with a sealing strip (14) along the circumferential direction.

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