A chemical detection device for immune cells

Abstract

This invention belongs to the technical field of detection equipment and provides a chemical detection device for immune cells, including an enzyme-linked immunosorbent assay (ELISA) reader. The ELISA reader contains a detection chamber with a detection placement plate inside. A sealing door is located on the side of the detection chamber. A protective box surrounding the detection chamber is fixedly installed on the side of the ELISA reader, and a cover plate is hinged to the side wall of the protective box. A frame-shaped mounting bracket is fixedly installed inside the protective box. A base plate is fixedly installed at one end of the mounting bracket, and a positioning plate is fixedly installed on the base plate. Side plates are fixedly installed on the side of the positioning plate, and mounting shafts are rotatably installed at both ends of the base plate. Compared with the prior art, the advantages of this invention are as follows: it enables rapid placement of microplates, avoids prolonged hand-holding of microplates by the testing personnel, prevents collisions between the microplates and the detection placement plate, and improves the stability of microplate placement.

Description

Technical Field

[0001] This invention belongs to the field of detection equipment technology, and in particular relates to a chemical detection device for immune cells. Background Technology

[0002] Using enzyme-linked immunosorbent assay (ELISA) to evaluate the level of glycoprotein E-specific binding antibodies induced by shingles vaccine is one of the gold standard methods in clinical research and vaccine efficacy evaluation.

[0003] The sample undergoes a chemical reaction with the substrate under the catalysis of enzymes, ultimately producing a colored product. The sample then needs to be detected by an ELISA reader. Existing ELISA readers have a specific groove structure for placing the microplate. Since there is a large amount of liquid inside the microplate, the staff needs to align it with the groove and pay attention to maintaining the balance of the microplate when placing it. Because the staff has poor stability when holding the microplate, it is easy for the microplate to collide with the groove, causing the solution in each well to flow and mix, resulting in inaccurate test results. Summary of the Invention

[0004] The purpose of this invention is to provide a chemical detection device for immune cells, which aims to solve the technical problem of the inability to quickly and stably place microplates in the prior art.

[0005] The present invention is implemented as follows: a chemical detection device for immune cells includes an enzyme-linked immunosorbent assay (ELISA) reader body, wherein a detection chamber is provided inside the ELISA reader body, a detection placement plate is provided inside the detection chamber, a closed door is provided on the side of the detection chamber, and a protective box surrounding the detection chamber is fixedly installed on the side of the ELISA reader body, and a cover plate is hinged to the side wall of the protective box.

[0006] The protective box is fixedly installed with a frame-shaped mounting frame. A base plate is fixedly installed at one end of the mounting frame. A positioning plate is fixedly installed on the base plate. A side plate is fixedly installed on the side of the positioning plate. Mounting shafts are rotatably installed at both ends of the base plate. Multiple sets of spaced support bars are fixedly installed on the mounting shafts. A first dual-axis motor that drives the two sets of mounting shafts to rotate is fixedly installed on the mounting frame.

[0007] An electric telescopic rod is fixedly installed on the surface of the mounting frame away from the cover plate. A lifting frame is fixedly installed at the output end of the electric telescopic rod. Multiple sets of spaced lifting plates are fixedly installed at the end of the lifting frame, and positioning holes are provided on the lifting plates.

[0008] A further technical solution: An observation window for observing the state inside the protective box is fixedly installed on the cover plate.

[0009] A further technical solution: The two output ends of the first dual-axis motor are rotatably connected to the corresponding mounting shafts through the first bevel gear, and the first dual-axis motor drives the two sets of mounting shafts to rotate synchronously in opposite directions.

[0010] A further technical solution: both ends of the substrate are rotatably mounted with mounting rings distributed around the mounting shaft. Support rods are fixedly mounted on the mounting rings, and multiple sets of connecting pipes are fixedly mounted on the support rods. The connecting pipes are rotatably connected to the mounting shaft and are spaced apart from the support bars. Support plates are fixedly mounted on the connecting pipes. A second dual-axis motor that drives the two sets of mounting rings to rotate is fixedly mounted on the substrate.

[0011] A further technical solution: The two output ends of the second dual-axis motor are rotatably connected to the corresponding mounting rings through the second bevel gear, and the second dual-axis motor drives the two sets of mounting rings to rotate synchronously in opposite directions.

[0012] A further technical solution: A rotating plate is rotatably mounted on the side of the positioning plate, a bracket is slidably mounted on the rotating plate, and a baffle plate is fixedly mounted at the end of the bracket.

[0013] A further technical solution: multiple sets of spaced limiting strips are slidably installed on the side of the shield.

[0014] A further technical solution: A dust removal fan and an air pump are fixedly installed on the bottom surface inside the protective box, and the air outlet of the air pump is connected to the outside.

[0015] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0016] 1. The microplate is positioned and initially placed using a positioning plate, side plate, and support strip. Then, the microplate is repositioned and placed for testing using a lifting plate. This achieves rapid placement of the microplate, avoids the need for the testing personnel to hold the microplate for a long time, prevents collisions between the microplate and the testing plate, improves the stability of the microplate placement, reduces the probability of mixing of solutions in different wells of the microplate, and improves the accuracy of the test results.

[0017] 2. The testing personnel can directly place the microplate on the plane formed by the support plate and the support strip 12. Then, the testing personnel can push the microplate to move on the plane and quickly position the microplate through the positioning plate and the side plate, which further reduces the time that the staff hold the microplate and further improves the placement speed and stability of the microplate, and reduces the difficulty of placing the microplate.

[0018] 3. The dust removal fan blows the air inside the protective chamber upwards, reducing the entry of external impurities. After the microplate is placed and the cover is closed, the air pump is turned on to extract the air from the protective chamber, further reducing dust and impurities. This also prevents residual dust and impurities from moving around, enabling rapid placement and sealing of the microplate. This reduces the contact time between the sample inside the microplate and external impurities. After sealing, the dust and impurities inside the protective chamber are cleaned, further reducing the probability of sample contact with impurities and improving the accuracy of the detection. Attached Figure Description

[0019] Figure 1 This is a schematic diagram of the overall structure of the present invention.

[0020] Figure 2 This is a schematic diagram of the internal structure of the protective box in this invention.

[0021] Figure 3 This is a schematic diagram of the protective box in this invention.

[0022] Figure 4 This is a first-view structural schematic diagram of the mounting bracket in this invention.

[0023] Figure 5 This is a second-view structural diagram of the mounting bracket in this invention.

[0024] Figure 6 This is a third-view structural diagram of the mounting bracket in this invention.

[0025] In the attached diagram: 1. Microplate reader body; 2. Sealed door; 3. Detection placement plate; 4. Protective box; 5. Dust removal fan; 6. Air pump; 7. Mounting frame; 8. Cover plate; 9. Observation window; 10. Base plate; 11. Mounting shaft; 12. Support bar; 13. First dual-axis motor; 14. First bevel gear; 15. Mounting ring; 16. Support rod; 17. Connecting pipe; 18. Support plate; 19. Second dual-axis motor; 20. Second bevel gear; 21. Electric telescopic rod; 22. Lifting frame; 23. Lifting plate; 24. Positioning hole; 25. Positioning plate; 26. Side plate; 27. Rotating plate; 28. Bracket; 29. ​​Shielding plate; 30. Limiting strip. Detailed Implementation

[0026] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the invention.

[0027] The specific implementation of the present invention will be described in detail below with reference to specific embodiments.

[0028] like Figures 1-6 As shown, this invention provides a chemical detection device for immune cells, including an enzyme-linked immunosorbent assay (ELISA) reader body 1, an ELISA reader body 1 with a detection chamber inside, a detection placement plate 3 inside the detection chamber, a sealing door 2 on the side of the detection chamber, a protective box 4 surrounding the detection chamber fixedly installed on the side of the ELISA reader body 1, a cover plate 8 hinged to the side wall of the protective box 4, and an observation window 9 fixedly installed on the cover plate 8 for observing the state inside the protective box 4.

[0029] A frame-shaped mounting bracket 7 is fixedly installed inside the protective box 4. A base plate 10 is fixedly installed at one end of the mounting bracket 7. A positioning plate 25 is fixedly installed on the base plate 10. A side plate 26 is fixedly installed on the side of the positioning plate 25. Mounting shafts 11 are rotatably installed at both ends of the base plate 10. Multiple sets of spaced support bars 12 are fixedly installed on the mounting shafts 11. A first dual-axis motor 13 is fixedly installed on the mounting bracket 7 to drive the two sets of mounting shafts 11 to rotate. The two output ends of the first dual-axis motor 13 are rotatably connected to the corresponding mounting shafts 11 through a first bevel gear 14. The first dual-axis motor 13 drives the two sets of mounting shafts 11 to rotate synchronously in opposite directions.

[0030] An electric telescopic rod 21 is fixedly installed on the surface of the mounting bracket 7 away from the cover plate 8. A lifting frame 22 is fixedly installed at the output end of the electric telescopic rod 21. Multiple sets of spaced lifting plates 23 are fixedly installed at the end of the lifting frame 22. Positioning holes 24 are provided on the lifting plates 23.

[0031] In practical application, the support strip 12 is initially horizontal. During testing, the cover plate 8 is opened, and the microplate carrying the enzyme-catalyzed reaction is placed on the support strip 12 for support. The spacing between the support strips 12 corresponds to the spacing between each row of holes in the microplate. When placing the microplate, one side of the microplate contacts the positioning plate 25, and the side plate 26 contacts both sides of the microplate, thereby positioning one end of the microplate. At this time, the microplate is placed downwards. Since the spacing between the support strips 12 corresponds to the spacing between each row of holes in the microplate, the microplate can be placed on the support strip 12. The support strip 12 is located between two rows of holes on the microplate, and the microplate is supported and further positioned by the support strip 12.

[0032] After placement, the cover plate 8 is closed. At this time, the sealing door 2 is opened, and the detection placement plate 3 moves into the protective box 4 to the bottom of the mounting frame 7. Then, the electric telescopic rod 21 drives the lifting frame 22 to move upward, causing the lifting plate 23 to move upward. The lifting plate 23 stops moving after passing through the detection placement plate 3 and contacting the microporous plate. Since the microporous plate is positioned by the positioning plate 25, the support bar 12, and the side plate 26, the outer wall of the multiple sets of holes on the microporous plate passes through the positioning hole 24.

[0033] The first dual-axis motor 13 drives two sets of mounting shafts 11 to rotate synchronously in opposite directions via the meshing first bevel gear 14, causing the support bar 12 to rotate to a vertical position. As the support bar 12 rotates, the microplate is supported and positioned by the lifting plate 23. Then, the electric telescopic rod 21 moves the microplate downwards via the lifting plate 23. After the microplate contacts the detection placement plate 3, its height is maintained. The lifting plate 23 continues to descend, separating from the microplate. At this point, the microplate is placed at the designated position on the detection placement plate 3. After placement, the detection placement plate 3 enters the microplate reader body 1, and the sealing door 2 closes. The microplate reader 1 performs the test on the sample. After the test is completed, the lifting plate 23 moves upward to push the microplate upward, and then the microplate can be removed. The microplate is positioned and initially placed by the positioning plate 25, side plate 26, and support strip 12. Then, the lifting plate 23 is used to reposition and place the microplate for testing. This achieves rapid placement of the microplate, avoids the tester holding the microplate for a long time, avoids collision between the microplate and the test placement plate 3, improves the stability of the microplate placement, reduces the probability of mixing of solutions in different wells of the microplate, and improves the accuracy of the test results.

[0034] like Figures 4-6 As shown, this invention provides a chemical detection device for immune cells. Mounting rings 15, distributed around a mounting shaft 11, are rotatably mounted at both ends of a substrate 10. Support rods 16 are fixedly mounted on the mounting rings 15, and multiple sets of connecting pipes 17 are fixedly mounted on the support rods 16. The connecting pipes 17 are rotatably connected to the mounting shaft 11 and are spaced apart from the support bars 12. A support plate 18 is fixedly mounted on the connecting pipes 17. A second dual-axis motor 19, which drives the two sets of mounting rings 15 to rotate, is fixedly mounted on the substrate 10.

[0035] Specifically, both output ends of the second dual-axis motor 19 are rotatably connected to the corresponding mounting rings 15 via the second bevel gear 20, and the second dual-axis motor 19 drives the two sets of mounting rings 15 to rotate synchronously in opposite directions.

[0036] In practical application, under dehumidification conditions, the support plate 18 is horizontal, forming a plane with the support bar 12. The support bar 12 has a sloping structure to facilitate smoother movement of the microporous plate during subsequent descent. During use, the testing personnel can directly place the microporous plate on the plane formed by the support plate 18 and the support bar 12. The testing personnel can then push the microporous plate to move on the plane and quickly position it using the positioning plate 25 and the side plate 26, further reducing the time spent by the staff holding the microporous plate and improving the placement speed and stability of the microporous plate, while reducing the difficulty of placing the microporous plate. After the microporous plate is positioned, the cover plate 8 can be closed. Subsequently, the second dual-axis motor 19 drives the two sets of mounting rings 15 to rotate through the meshing second bevel gear 20. The mounting rings 15 drive the support plate 18 to rotate to a vertical position through the support rod 16. At this time, the microporous plate will move downward and be supported by the support bar 12. The support bar 12 prevents the microporous plate from moving laterally, ensuring accurate positioning of the microporous plate. Subsequently, the lifting plate 23 can be used to move the microporous plate.

[0037] like Figure 4 , Figure 6 As shown, this invention provides a chemical detection device for immune cells. A rotating plate 27 is rotatably mounted on the side of the positioning plate 25. A bracket 28 is slidably mounted on the rotating plate 27. A shielding plate 29 is fixedly mounted at the end of the bracket 28.

[0038] Specifically, multiple sets of spaced limiting strips 30 are slidably installed on the side of the shield 29.

[0039] Specifically, a dust removal fan 5 and an air pump 6 are fixedly installed on the bottom of the protective box 4, and the air outlet of the air pump 6 is connected to the outside.

[0040] In practical application, after the cover plate 8 is opened, the dust removal fan 5 is turned on, blowing the air inside the protective box 4 upwards, thereby reducing the entry of external impurities into the protective box 4. After the cover plate 8 is closed, the dust removal fan 5 is turned off. After the microporous plate is positioned on the plane formed by the support plate 18 and the support strip 12, the rotating plate 27 is rotated to a horizontal state. Under the action of gravity, the shielding plate 29 covers the microporous plate. At this time, the limiting strip 30 contacts the support plate 18 and the side of the microporous plate. Then, the air pump 6 is turned on to extract the air from the protective box 4, further reducing the dust inside the protective box 4. To remove impurities and prevent residual dust and impurities inside the protective box 4 from moving freely, the support plate 18 is rotated to a vertical position. When the support plate 18 rotates, the microplate moves downward. The position of the microplate is further restricted by the limiting strip 30 to prevent the microplate from tilting and ensure its stability. After dust removal, the microplate moves downward away from the shielding plate 29, realizing rapid placement and sealing of the microplate, reducing the contact time between the sample inside the microplate and external impurities. At the same time, after sealing, the dust and impurities inside the protective box 4 are cleaned, further reducing the probability of sample contact with impurities and further improving the accuracy of detection.

[0041] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

[0042] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A chemical detection device for immune cells, comprising an enzyme-linked immunosorbent assay (ELISA) reader body (1), characterized in that, The microplate reader body (1) is provided with a detection chamber, and a detection placement plate (3) is provided inside the detection chamber. A closed door (2) is provided on the side of the detection chamber. A protective box (4) surrounding the detection chamber is fixedly installed on the side of the microplate reader body (1). A cover plate (8) is hinged to the side wall of the protective box (4). The protective box (4) is fixedly installed with a frame-shaped mounting bracket (7). A base plate (10) is fixedly installed at one end of the mounting bracket (7). A positioning plate (25) is fixedly installed on the base plate (10). A side plate (26) is fixedly installed on the side of the positioning plate (25). Mounting shafts (11) are rotatably installed at both ends of the base plate (10). Multiple sets of spaced support bars (12) are fixedly installed on the mounting shafts (11). A first dual-axis motor (13) that drives the two sets of mounting shafts (11) to rotate is fixedly installed on the mounting bracket (7). An electric telescopic rod (21) is fixedly installed on the surface of the mounting frame (7) away from the cover plate (8). A lifting frame (22) is fixedly installed at the output end of the electric telescopic rod (21). Multiple sets of spaced lifting plates (23) are fixedly installed at the end of the lifting frame (22). Positioning holes (24) are provided on the lifting plates (23).

2. The chemical detection device for immune cells according to claim 1, characterized in that, An observation window (9) for observing the state inside the protective box (4) is fixedly installed on the cover plate (8).

3. The chemical detection device for immune cells according to claim 1, characterized in that, The two output ends of the first dual-axis motor (13) are rotatably connected to the corresponding mounting shaft (11) through the first bevel gear (14), and the first dual-axis motor (13) drives the two sets of mounting shafts (11) to rotate synchronously in opposite directions.

4. The chemical detection device for immune cells according to claim 1, characterized in that, Mounting rings (15) are rotatably mounted on both ends of the substrate (10) and distributed around the mounting shaft (11). Support rods (16) are fixedly mounted on the mounting rings (15). Multiple sets of connecting pipes (17) are fixedly mounted on the support rods (16). The connecting pipes (17) are rotatably connected to the mounting shaft (11) and are spaced apart from the support bars (12). Support plates (18) are fixedly mounted on the connecting pipes (17). A second dual-axis motor (19) that drives the two sets of mounting rings (15) to rotate is fixedly mounted on the substrate (10).

5. The chemical detection device for immune cells according to claim 4, characterized in that, The two output ends of the second dual-axis motor (19) are rotatably connected to the corresponding mounting rings (15) through the second bevel gear (20), and the second dual-axis motor (19) drives the two sets of mounting rings (15) to rotate synchronously in opposite directions.

6. The chemical detection device for immune cells according to claim 1, characterized in that, The positioning plate (25) has a rotating plate (27) rotatably mounted on its side, and a bracket (28) is slidably mounted on the rotating plate (27). A baffle plate (29) is fixedly mounted on the end of the bracket (28).

7. The chemical detection device for immune cells according to claim 6, characterized in that, The side of the shield (29) is slidably fitted with multiple sets of spaced limiting strips (30).

8. The chemical detection device for immune cells according to claim 7, characterized in that, The protective box (4) is fixedly installed with a dust removal fan (5) and an air pump (6) on the bottom surface. The air outlet of the air pump (6) is connected to the outside.

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