沙门氏菌噬菌体RNA聚合酶的高密度发酵工艺
By optimizing the culture medium and feeding strategy, the fed-batch fermentation technology has solved the problem of long fermentation cycle of Salmonella bacteriophage RNA polymerase, achieving high-density fermentation, improving enzyme yield and production efficiency, and meeting the needs of industrial applications.
CN121555461BActive Publication Date: 2026-07-17DALIAN HANWEI CHICKEN FARM +1
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- DALIAN HANWEI CHICKEN FARM
- Filing Date
- 2025-11-25
- Publication Date
- 2026-07-17
AI Technical Summary
Technical Problem
In existing technologies, the fermentation cycle of Salmonella bacteriophage RNA polymerase depends on the lysis rate of the host bacteria, resulting in a longer cycle and reduced potency, which cannot meet the needs of industrial production.
Method used
By optimizing the culture medium and feeding strategy, and adopting fed-batch fermentation technology, the induction timing and conditions are precisely controlled to improve cell wet weight and enzyme activity, thereby achieving high-density fermentation.
Benefits of technology
It significantly increases enzyme yield per unit fermentation volume, shortens the fermentation cycle, reduces energy consumption and costs, and adapts to diversified production needs.
✦ Generated by Eureka AI based on patent content.
Smart Images
Figure CN121555461B_ABST
Abstract
本发明公开了沙门氏菌噬菌体RNA聚合酶的高密度发酵工艺，属于沙门氏菌技术领域。沙门氏菌噬菌体RNA聚合酶的高密度发酵工艺，包括以下步骤：S1：种子液制备；S2：种子扩大培养；S3：高密度发酵；S4：诱导表达；S5：酶提取纯化。本发明解决了现有技术培养周期完全依赖宿主菌裂解速度，若需缩短周期，只能通过提高初始噬菌体感染复数，但会导致宿主菌过早裂解，效价进一步降低。本发明通过优化培养基与补料策略，使得菌体湿重大幅度提升，单位发酵体积酶产量显著提高，提高了菌体酶活比和纯度，有效地缩短了发酵周期，提高了生产效率，能够灵活切换短周期中产量或长周期高产量模式，以适配多样化生产需求。
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