敲除SlABCB13基因在提高番茄果实可溶性糖含量中的应用
By knocking out the SlABCB13 gene and using the CRISPR/Cas9 system to increase the soluble sugar content of tomato fruits, the problem of balancing high yield and high quality was solved, and a significant increase in soluble sugar in the fruits was achieved.
CN121555562BActive Publication Date: 2026-07-17JILIN AGRICULTURAL UNIV
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- JILIN AGRICULTURAL UNIV
- Filing Date
- 2026-01-13
- Publication Date
- 2026-07-17
AI Technical Summary
Technical Problem
In current tomato breeding practices, it is difficult to achieve both high yield and high quality (especially soluble sugar content), resulting in a decline in fruit flavor and quality.
Method used
By knocking out the SlABCB13 gene and designing specific sgRNA using the CRISPR/Cas9 system to target and cleave the SlABCB13 gene, resulting in its loss of function, the soluble sugar content in tomato fruit can be increased.
Benefits of technology
It significantly increased the soluble sugar content in tomato fruits, including fructose, glucose and sucrose, without affecting yield indicators such as plant height, stem diameter, fruit size, number of fruits set and yield per plant, providing genetic resources for breeding high-sugar tomatoes.
✦ Generated by Eureka AI based on patent content.
Smart Images
Figure CN121555562B_ABST
Abstract
本发明涉及基因工程技术领域，特别是涉及敲除SlABCB13基因在提高番茄果实可溶性糖含量中的应用。通过介导基因编辑使SlABCB13基因功能缺失，显著提高番茄果实中的可溶性糖含量（蔗糖、果糖、葡萄糖）和可溶性固形物含量，表明SlABCB13参与了番茄果实糖转运。同时，突变体不影响株高、茎粗、果实大小、果实坐果数和单株果实产量等相关产量指标，对现有的番茄育种中产量与品质难以兼顾的难题有了新思路。
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