An apparatus for studying the allelopathic effects between plants

Abstract

The application relates to the technical field of plant allelopathy effect, and discloses a device for studying plant interallelopathy effect, which comprises a culture box, and an alternating exchange bin is connected to the top of the culture box; an alternating exchange structure for conducting allelopathy exchange between two observation rooms is arranged in the alternating exchange bin. If it is needed to collect the allelopathy substance of the current growth stage of the plants in which observation room, the ball valve of the air pipe without a branch pipe installed in the observation room needed to collect the allelopathy substance is opened, then the three-way valve installed in the other observation room is opened, so that the airflow direction can only flow into the air bag, then the motor is started to drive the fan to rotate, the allelopathy substance in the observation room is extracted into the inside of the air bag, when the allelopathy substance in the observation room is collected through the air bag, the airflow passes through the filter sheet, the allelopathy substance in the airflow is adsorbed by the elution layer on the surface of the filter sheet, and the beneficial effect that the allelopathy substance released into the air by the plants can be collected in real time during the whole growth period or different growth stages of the plants is achieved.

Description

Technical Field

[0001] This invention relates to the field of plant allelopathic effects technology, specifically to a device for studying allelopathic effects among plants. Background Technology

[0002] During plant growth, plants can transmit information to surrounding plants through allelopathy, thereby influencing the growth and development, photosynthesis, respiration, endogenous hormone levels, metabolic content, and plant responses to adversity.

[0003] Currently, in the study of plant allelopathic effects, the plant material is usually ground, and then the plant's secondary metabolites are extracted. These secondary metabolites are then used as allelochemicals to study allelopathic effects. For example, the specification of CN120283791A, paragraph 0018, discloses that "the biological antibacterial agent includes bean root exudates, and the concentration of the bean root exudates is 66.67–142.86 g / L." As a specific implementation method, the concentration of the bean root exudates is calculated as follows: 100 g of beans are soaked, germinated, and hydroponically cultured until the bean roots reach a length of 5–7 cm. The roots are then... Beans with a root length of 5-7 cm were cultured in 1500 mL of sterilized deionized water for 24 hours, and the resulting bean root exudate was defined as having an initial concentration of 66.67 g / L. As another specific embodiment, 100 g of beans were soaked, germinated, and hydroponically cultured until the bean roots reached a length of 5-7 cm. Beans with roots of 5-7 cm were then cultured in 700 mL of sterilized deionized water for 24 hours, and the resulting bean root exudate was defined as having an initial concentration of 142.86 g / L. As another specific embodiment, the bean root length can be any of 5 cm, 6 cm, or 7 cm.

[0004] Although plant allelochemicals are relatively small-molecule, simple-structured secondary metabolites, the composition and proportion of these allelochemicals vary depending on the plant's growth stage, growth conditions, and abiotic stresses, leading to alterations in the chemical signals released. Therefore, conventional methods of studying allelopathic effects by extracting secondary metabolites from plants are insufficient to accurately reflect the allelopathic interactions between plants under different abiotic conditions. Summary of the Invention

[0005] Technical problems to be solved: To address the shortcomings of existing technologies, this invention provides a device for studying allelopathic effects among plants. This device is capable of collecting allelopathic substances released into the air by plants in real time throughout their entire growth period or at different growth stages, releasing the collected allelopathic substances into the air, and observing the advantages of the allelopathic effects of the plants, thus solving the problems mentioned above.

[0006] Technical solution: To achieve the above objectives, the present invention provides the following technical solution: a device for studying allelopathy among plants, comprising a culture chamber, wherein an isolation plate is vertically installed in the middle of the inner side of the culture chamber, the isolation plate and the four sides of the culture chamber are sealed, the inner side of the culture chamber is divided into two observation rooms for placing plants by the isolation plate, each of the two observation rooms is equipped with a hinged door, the hinged door is located on the front wall of the culture chamber, and each of the two observation rooms, i.e. the top surface of the inner side of the culture chamber, has an air hole penetrating the top surface of the culture chamber, each observation room having at least two air holes, the top of the air hole is open and located on the top surface of the culture chamber and connected to an air pipe by a flange, a ball valve is movably installed at the bottom of the air pipe, the top of the air pipe extends towards the top of the culture chamber and the tail end is connected to an AC compartment; The communication chamber is equipped with a communication structure for allelopathic communication between the two observation rooms; Includes a fan, which is located in the middle of the inner side of the communication chamber. There are two observation rooms on the left and right sides of the fan, and air pipes extending from the two observation rooms are connected to the front and rear of the fan. It also includes an extension tube, which is installed at the end of the trachea and inserted into the inside of the communication chamber. At least one of the two tracheas extending from each observation room has an extension tube installed at its end. A three-way valve is installed in the middle of the extension tube, with the two ends of the three-way valve connected to the extension tube and the last end connected to the airbag.

[0007] Preferably, the incubator is made of tempered glass or acrylic sheet, and is bidirectionally transparent, and is bonded together with glass glue.

[0008] Preferably, the three-way valve connecting to one end of the airbag further includes a branch pipe, which is a hollow pipe with a fixed threaded sleeve at the tail end. The threaded sleeve is also hollow and its tail end forms a sealed connection with the tail end of the branch pipe. The tail end of the threaded sleeve is threadedly connected to a screw ring fixedly installed on the front wall of the airbag. The inner side of the screw ring is also hollow and its tail end is fixedly connected to an opening on the front wall of the airbag. The outer surface of the screw ring is threadedly connected to the inner side of the threaded sleeve, and a filter is inserted into the inner side of the screw ring.

[0009] Preferably, the filter surface is further coated with an elution layer, which is one of Tenax-TA, Tenax-GR, activated carbon, and silica gel, and the thickness of the elution layer is 0.1 mm.

[0010] Preferably, the fan's shaft is also fixedly connected to the output end of a motor, and the motor is mounted on the outer surface of the AC compartment.

[0011] Preferably, the communication chamber is an internally hollow cylinder perpendicular to the top surface of the incubator, and the top and bottom surfaces on both sides of the communication chamber are also sealed and connected to four air tubes extending from the two observation rooms.

[0012] Preferably, the communication structure further includes four air pipes extending from the two observation rooms to support the communication chamber. One air pipe from each observation room is connected to the bottom surface of the communication chamber to form a vertical shape, and the other air pipe is connected to the top surface of the communication chamber to form an inverted "L" shape. The four air pipes and the communication chamber together form an M-shaped support for the communication chamber.

[0013] Preferably, the communication chamber is an internally hollow cylinder parallel to the top surface of the incubator. The front and rear ends of the two sides of the communication chamber are also sealed and connected to four air tubes extending from the two observation rooms. The tail ends of the four air tubes are all connected to extension tubes, but only one of the two air tubes extending from each observation room has an air bag installed on the extension tube at the tail end of the air tube.

[0014] Preferably, the AC structure further includes a support bracket, the top of which is an arc shape that fits the bottom surface of the AC compartment, and the bottom of which is vertically connected to the top surface of the AC compartment.

[0015] Preferably, the fan is located at the center of the inner side of the AC chamber, which is parallel to the top surface of the incubator, and each of the front and rear ends of the fan has an extension tube opening located inside the AC chamber.

[0016] Compared with the prior art, the present invention provides an apparatus for studying allelopathic effects among plants, which has the following beneficial effects: 1. This invention locks the trachea by closing all the ball valves of the four trachea. Then, to collect allelochemicals from the plant at the current growth stage in the observation room where the allelochemicals need to be collected, the ball valve of the trachea without a branch pipe in that observation room is opened. Next, the three-way valve of the trachea with a branch pipe in the other observation room is opened, ensuring that the airflow can only flow into the airbag. At this point, the front and rear ends of the branch pipe are indirectly connected to one observation room and one airbag, respectively. Then, the motor is started to drive the fan. It is important to note that the direction of fan rotation must ensure that the rear end is connected to the trachea without a branch pipe. In this state, the fan creates a pump-like effect between the observation room and the airbag. The allelochemicals from the observation chamber are extracted into the inside of the airbag. As the airbag collects the allelochemicals, the airflow passes through a filter. The elution layer on the surface of the filter adsorbs the allelochemicals in the airflow. After collection, the airbag can be either disassembled or injected into another observation chamber. If disassembly is chosen, the airbag is rotated in the opposite direction to cause relative movement between the spiral ring and the sleeve at its front end, causing the spiral ring to detach from the sleeve. At this point, the filter inside the spiral ring is exposed. The filter now adsorbs the allelochemicals from the observation chamber and can be directly removed for further research. This achieves the beneficial effect of collecting allelochemicals released into the air by plants in real time throughout the entire growth period or at different growth stages.

[0017] 2. This invention involves removing the filter and closing all the ball valves of the four air tubes to lock them. Then, to collect allelochemicals from the plant at the current growth stage in a particular observation room, the ball valve of the air tube without a branch pipe in that observation room is opened. Next, the three-way valve of the air tube with a branch pipe in another observation room is opened, ensuring airflow only into the airbag. The front and rear ends of the branch pipe are indirectly connected to one observation room and one airbag, respectively. The motor is then started to drive the fan. It is important to note that the fan's rotation direction must ensure that the rear end connects to the air tube without a branch pipe. The fan creates a pump-like effect between the observation room and the airbag, drawing the allelochemicals from that observation room into the airbag. The ball valve of the air tube without a branch pipe in the first observation room is then closed, and the ball valve of the air tube without a branch pipe in the second observation room is opened. The allelochemicals collected in the first observation room then enter the second observation room, achieving the beneficial effect of releasing the collected allelochemicals into the air and observing the allelochemical effects of plants. Attached Figure Description

[0018] Figure 1 This is a schematic diagram of the overall structure of Embodiment 1 of the present invention; Figure 2 This is a schematic cross-sectional view of the overall structure of Embodiment 1 of the present invention; Figure 3 This is a schematic cross-sectional view of the AC compartment structure according to Embodiment 1 of the present invention; Figure 4 This is a schematic cross-sectional view of the branch pipe structure of the present invention; Figure 5 This is a schematic diagram of the overall structure of Embodiment 2 of the present invention; Figure 6 This is a schematic cross-sectional view of the overall structure of Embodiment 2 of the present invention; Figure 7 This is a schematic cross-sectional view of the AC compartment structure according to Embodiment 2 of the present invention; Figure 8 This is a cross-sectional view of the trachea structure in Embodiment 2 of the present invention.

[0019] The components include: 1. Incubator; 101. Isolation plate; 2. Air vent; 3. Air tube; 301. Ball valve; 4. Exchange chamber; 401. Fan; 5. Extension tube; 501. Three-way valve; 502. Branch tube; 503. Screw sleeve; 6. Airbag; 601. Threaded ring; 602. Filter plate; 7. Support bracket. Detailed Implementation

[0020] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0021] Please see Figure 1-8 An apparatus for studying allelopathy among plants includes an incubator 1. An isolation plate 101 is vertically installed in the middle of the inner side of the incubator 1. The isolation plate 101 and the four sides of the incubator 1 form a seal. The inner side of the incubator 1 is divided into two observation rooms for placing plants by the isolation plate 101. Each of the two observation rooms is equipped with a hinged door located on the front wall of the incubator. The top of each observation room, i.e., the top surface of the inner side of the incubator 1, is provided with an air hole 2 that penetrates the top surface of the incubator 1. Each observation room has at least two air holes 2. The top of the air hole 2 is open and located on the top surface of the incubator 1. An air pipe 3 is connected to the air pipe 3 through a flange. A ball valve 301 is movably installed at the bottom of the air pipe 3. The top of the air pipe 3 extends towards the top of the incubator 1 and the tail end is connected to an AC chamber 4. Two observation rooms are located on each side of the communication chamber 4. The communication chamber 4 is equipped with an communication structure for allelopathic communication between the two observation rooms. Includes fan 401, with two air tubes 3 extending from two observation rooms connected to the front and rear of the left and right sides of fan 401; It also includes an extension tube 5, which is installed at the end of the air tube 3 and inserted into the inside of the communication chamber 4. At least one of the two air tubes 3 extending from each observation room has an extension tube 5 installed at its end. A three-way valve 501 is installed in the middle of the extension tube 5. The two ends of the three-way valve 501 are connected to the extension tube 5 respectively, and the last end is connected to the airbag 6.

[0022] Hinged doors are the most basic function of incubator 1 in the laboratory and are the most common facility for those skilled in the art. Their purpose is to open incubator 1. They can be purchased directly from the market, as shown in product number 10195563703492, so they will not be described in detail.

[0023] Furthermore, the incubator 1 is made of tempered glass or acrylic sheet, and is transparent in both directions, and is bonded together with glass glue.

[0024] By using tempered glass or acrylic sheets, which are transparent inside and out, the incubator 1 can be made transparent, so as to take pictures or record screen data. At the same time, the glass glue is an existing silicone sealant, which, after curing, expands and contracts with the glass due to thermal expansion and contraction, and will not crack. Since the use of glass glue to bond tempered glass or acrylic sheets is a very common application in the field, and this application has not improved it, the bonding steps of the glass glue will not be described in detail.

[0025] Furthermore, the three-way valve 501 also includes a branch pipe 502 at one end connected to the airbag 6. The branch pipe 502 is a hollow pipe with a fixed connection to the tail end of the branch pipe 502. The screw sleeve 503 is also hollow and its tail end forms a sealed connection with the tail end of the branch pipe 502. The tail end of the screw sleeve 503 is threadedly connected to a screw ring 601 fixedly installed on the front wall of the airbag 6. The inner side of the screw ring 601 is also hollow and its tail end is fixedly connected to the opening on the front wall of the airbag 6. The outer surface of the screw ring 601 is threadedly connected to the inner side of the screw sleeve 503. A filter 602 is inserted into the inner side of the screw ring 601.

[0026] Airbag 6 is a rubber airbag, which is a product that can be purchased directly on the online shopping platform. Its product number is 10086338841781. Airbag (6) can be replaced with different sizes according to the purpose of the experiment. However, the disassembly and assembly methods of the airbag are the same. They are all fixed by screwing together the screw sleeve 503 and the screw ring 601.

[0027] The airbag 6 connected to the end of the branch pipe 502 of the three-way valve 501 is used to collect allelochemicals from the two observation rooms. This allows the device to not only exchange allelochemicals but also collect them. Specifically, all the ball valves 301 of the four air pipes 3 are closed to lock the air pipes 3. Then, to collect allelochemicals from the observation room that needs to be collected, the ball valve 301 of the air pipe 3 of the observation room that does not have the branch pipe 502 installed is opened. Then, the three-way valve 501 of the other observation room that has the branch pipe 502 installed is opened, so that the airflow can only flow into the airbag 6. At this time, the branch pipe... The front and rear ends of 502 are indirectly connected to an observation room and an airbag 6, respectively. Then, the motor is started to drive the fan 401 to rotate. It is worth noting that the direction of the fan rotation must ensure that the air pipe 3 connected to the rear is not connected to the branch pipe 502. At this time, the fan 401 forms a pump-like effect between the observation room and the airbag 6, drawing the allergenic material from the observation room into the inside of the airbag 6. It is worth noting that the airbag 6 draws allergenic material in a cross manner in this application, that is, when the allergenic material from the left observation room is drawn, the three-way valve 501 above the right observation room, which is equipped with the branch pipe 502, is opened.

[0028] Furthermore, the surface of filter 602 is coated with an eluent layer, which is one of Tenax-TA, Tenax-GR, activated carbon, and silica gel, and the thickness of the eluent layer is 0.1 mm.

[0029] Tenax-TA and Tenax-GR are heat resistant (≤300℃), hydrophobic, and have strong adsorption capacity for C6-C20 allelochemical VOCs such as terpenes and phenols. They are also easily thermally desorbed and suitable for gas chromatography (GC) analysis. Activated carbon has a wide adsorption range, from low-boiling-point to high-boiling-point VOCs, and is low in cost, but it is easily decomposed by thermal desorption. It is used to enrich low-concentration, difficult-to-adsorb allelochemical gases such as small molecule aldehydes. Silica gel is used to adapt to polar allelochemical gases such as alcohols and ketones. When collecting allelochemical substances in the observation room, the gas flow passes through filter 602 via the gasbag 6, and the allelochemical substances in the gas flow are adsorbed by the eluent layer on the surface of filter 602.

[0030] Furthermore, the shaft of fan 401 is also fixedly connected to the output end of a motor, which is mounted on the outer surface of AC compartment 4.

[0031] The motor is a 12V 4000 RPM model with the existing model number WS-555R. Its encoder is a magnetic encoder, which is a micro motor capable of forward and reverse rotation. Since it is a device that can be purchased directly on shopping platforms, the specific operation and installation methods are already publicly available. Furthermore, this application has not made any improvements to the electrodes, but only uses them as a power source to directly connect to the shaft of the fan for driving. Therefore, it will not be described in detail.

[0032] Furthermore, the exchange chamber 4 is an internally hollow cylinder perpendicular to the top surface of the incubator 1, and the top and bottom surfaces on both sides of the exchange chamber 4 are also sealed and connected to four air tubes 3 extending from the two observation rooms.

[0033] As per the instruction manual Figure 2-3 The two observation rooms are interconnected by four air pipes 3 extending from the top and bottom surfaces of the two sides of the exchange chamber 4, which are sealed and connected respectively. In conjunction with the aforementioned technical solution, a ball valve 301 is installed at the bottom of each air pipe 3. This allows for various combinations: if all four ball valves 301 are closed, the two observation rooms become completely independent and do not interfere with each other; if all four ball valves 301 are open, the two air pipes 3 in each observation room complete the chemiluminescent exchange of gases through the exchange chamber 4. Furthermore, by sequentially closing one air pipe 3 on each side of the top and bottom surfaces of the exchange chamber 4, the remaining air pipes 3 in the two observation rooms are positioned such that one is on the top surface of the exchange chamber 4 and the other on the bottom surface, thus creating interconnection. This is coordinated with the motor for forward and reverse rotation. If the air pipe 3 on the left side of the exchange chamber is on its top surface and the air pipe 3 on the right side is on its bottom surface, then the forward fan 401 rotates as shown in the instruction manual. Figure 2As shown, the fan 401 compresses the gas behind it, which is the gas drawn from the left air pipe 3. This corresponds to the gas in the left observation room being drawn into the exchange chamber 4. Then, the space in front of the fan, i.e., the right air pipe 3, is powered by the air output from the fan 401, carrying the gas from the left observation room into the right observation room, thus completing the exchange of chemiluminescent substances. Furthermore, if the gas in the right observation room is to be introduced into the left observation room, the reverse motor drives the fan 401 to reverse, or one air pipe 3 on the top and one on the bottom of each side of the exchange chamber 4 are closed in sequence, so that the air pipe 3 on the left side of the exchange chamber is on its bottom surface and the air pipe 3 on the right side is on its top surface, the forward motor continues to drive the fan 401 to rotate in the forward direction.

[0034] Furthermore, the communication structure also includes four air pipes 3 extending from the two observation rooms to support the communication chamber 4. One air pipe 3 extending from a single observation room is connected to the bottom surface of the communication chamber 4 to form a vertical shape, and the other air pipe 3 is connected to the top surface of the communication chamber 4 to form an inverted "L" shape. The four air pipes 3 and the communication chamber 4 together form an M-shaped support for the communication chamber 4.

[0035] The two air pipes 3 at the bottom of the exchange chamber 4 extend towards the exchange chamber 4 and directly contact the top surface of the incubator 1, forming a support for the exchange chamber 4 based on the incubator 1.

[0036] Furthermore, the exchange chamber 4 is an internally hollow cylinder parallel to the top surface of the incubator 1. The front and rear ends of the two sides of the exchange chamber 4 are also sealed and connected to four air tubes 3 extending from the two observation rooms. The tail ends of the four air tubes 3 are all connected to extension tubes 5, but only one of the two air tubes 3 extending from each observation room has an airbag 6 installed on the extension tube 5 at the tail end of the air tube 3.

[0037] As per the instruction manual Figure 6-7 The two observation rooms are interconnected by four air pipes 3 extending from the front and rear ends of the two sides of the exchange chamber 4, which are sealed and connected respectively. In conjunction with the aforementioned technical solution, a ball valve 301 is installed at the bottom of each air pipe 3. This allows for various combinations: if all four ball valves 301 are closed, the two observation rooms become completely independent and do not interfere with each other; if all four ball valves 301 are open, the two air pipes 3 in each observation room complete the chemiluminescent exchange of gases through the exchange chamber 4. Furthermore, by sequentially closing one air pipe 3 at the front and one at the rear of each side of the exchange chamber 4, the remaining air pipes 3 in the two observation rooms are positioned such that one is at the front of the exchange chamber 4 and the other at the rear, thus creating interconnection. This is coordinated with the motor for forward and reverse rotation. If the air pipe 3 on the left side of the exchange chamber is at its front and the air pipe 3 on the right side is at its rear, then the forward fan 401 rotates as shown in the instruction manual. Figure 2As shown, the fan 401 compresses the gas behind it, which is the gas drawn from the right-side air pipe 3 at the rear end. This corresponds to the gas in the right observation room being drawn into the exchange chamber 4. Then, the space in front of the fan, i.e., the air pipe 3 on the left side, is powered by the air output from the fan 401, carrying the gas from the right observation room into the left observation room, thus completing the exchange of allelochemical substances. Further, if the gas in the left observation room is to be introduced into the right observation room, the reverse motor drives the fan 401 to reverse, or one air pipe 3 at the front end and one air pipe 3 at the rear end on both sides of the exchange chamber 4 are closed in sequence, so that the air pipe 3 on the left side of the exchange chamber is at its rear end and the air pipe 3 on the right side is at its front end, the forward motor continues to drive the fan 401 to rotate forward.

[0038] Furthermore, the communication structure also includes a support bracket 7, the top of which is an arc shape that fits the bottom surface of the communication compartment 4, and the bottom of the support bracket 7 is vertically connected to the top surface of the communication compartment 4.

[0039] Furthermore, the fan 401 is located at the center of the inner side of the exchange chamber 4, which is parallel to the top surface of the incubator 1, and each of the front and rear ends of the fan 401 has an extension tube 5 with an opening inside the exchange chamber 4.

[0040] Example 1 In this embodiment, the incubator 1 is made of tempered glass or acrylic sheet, and is bidirectionally transparent, and is bonded with glass glue. In addition, which is not shown in the attached drawings, the outer surface of the incubator 1 is also fitted with reinforcing ribs. Since the addition of reinforcing ribs to the surface of the glass box for explosion prevention is a common technical solution and not a technical improvement, it will not be described in detail. In this embodiment, the elution layer on the surface of the filter 602 is: Tenax-T or Tenax-GR, with a thickness of 0.1mm. In this embodiment, the three-way valve 501 is kept in the normally closed state of the airbag 6 by default.

[0041] As per the instruction manual Figure 1As shown, the front wall of the incubator 1 in this application is equipped with a hinged door. Plants are taken out or put in by opening the hinged door. After the plants are placed in the observation room, the hinged door is closed. To ensure the airtightness of subsequent experiments, a polyethylene film can be used to cover the hinged door. When two plants that need to be subjected to allelopathic experiments are put into incubator 1, the external lights of the laboratory can be turned on to provide supplemental lighting for the plants and promote their photosynthesis. Furthermore, as the plants produce oxygen through photosynthesis, this oxygen contains allelopathic substances from the plants. At this time, the allelopathic substances can be collected and discharged through the branch pipe 502 connected to the three-way valve 501. The airbag 6 connected to the tail end is used to collect allelochemicals from the two observation rooms. All ball valves 301 on the four air tubes 3 are closed to lock the air tubes 3. Then, to collect allelochemicals from the plant at the current growth stage in the observation room where collection is needed, the ball valve 301 on the air tube 3 of the observation room without branch pipes 502 is opened. Then, the three-way valve 501 of the other observation room with branch pipes 502 is opened, ensuring that airflow only flows into the airbag 6. At this point, the front and rear ends of the branch pipes 502 are indirectly connected to one observation room and one airbag 6, respectively. Then, the motor is started to drive the fan 401 to rotate. It is important to note that the direction of fan rotation must ensure that the air pipe 3 connected to the rear is not connected to the branch pipe 502. At this time, the fan 401 creates a pump-like effect between the observation room and the airbag 6, drawing the allelochemicals from the observation room into the inside of the airbag 6. When the airbag 6 collects the allelochemicals from the observation room, the airflow passes through the filter 602, and the allelochemicals in the airflow are adsorbed by the elution layer on the surface of the filter 602. After collection, the airbag 6 can be either disassembled or injected into another observation room. If disassembly is chosen, the airbag 6 is rotated in the opposite direction so that the screw ring 601 at its front end moves relative to the screw sleeve 503. The screw ring 601 is disengaged from the screw sleeve 503, exposing the filter 602 inside the screw ring 601. The filter 602 has adsorbed the allelochemical in the observation room and can be removed directly for further research. The removed filter 602 is heated by electricity to release the gas containing the allelochemical and then collected separately. The collected gas containing the allelochemical is then sent back into the observation room. The disassembled airbag 6 is deflated and then connected to the branch pipe 502. To prevent negative pressure from being generated in the observation room where the allelochemical is extracted, the fan 401 can be reversed to replenish the airbag 6 when it leaves the branch pipe 502.

[0042] Furthermore, this embodiment can also release the collected allelochemicals. First, the airbag 6 is rotated in the opposite direction so that the screw ring 601 at its front end moves relative to the screw sleeve 503, causing the screw ring 601 to disengage from the screw sleeve 503. At this time, the filter 602 inside the screw ring 601 is exposed. The filter 602 of the airbag 6 is removed separately. The aforementioned technical solution is repeated to reinstall the airbag 6 into the branch pipe 502. All the ball valves 301 of the four air pipes 3 are closed to lock the air pipes 3. Then, to collect allelochemicals of the current growth stage of the plants in the observation room, the ball valve 301 of the air pipe 3 of the observation room where the allelochemicals need to be collected is opened, and then the three-way valve 5 of the other observation room where the branch pipe 502 is installed is opened. 01, ensuring that the airflow can only flow into the airbag 6. At this time, the front and rear ends of the branch pipe 502 are indirectly connected to an observation room and an airbag 6, respectively. Then, the motor is started to drive the fan 401 to rotate. It is worth noting that the direction of the fan rotation must ensure that the air pipe 3 without the branch pipe 502 is connected to the rear. At this time, the fan 401 forms a pump-like effect between the observation room and the airbag 6, drawing the allelochemicals from the observation room into the inside of the airbag 6. Then, the ball valve 301 of the air pipe 3 without the branch pipe 502 in the observation room is closed, and the ball valve 301 of the air pipe 3 without the branch pipe 502 in the other observation room is opened. At this time, the plant allelochemicals collected in the observation room enter the other observation room.

[0043] Furthermore, the experiment not only involves collecting allelochemicals, but sometimes also requires mixing allelochemicals from two observation rooms to observe plant growth. Four air pipes 3 extending from the two observation rooms are sealed and connected to the top and bottom surfaces of the two sides of the exchange chamber 4, forming an interconnection between the two observation rooms. Combining the aforementioned technical solution, each air pipe 3 has a ball valve 301 installed at its bottom. This allows for various combinations: if all four ball valves 301 are closed, the two observation rooms become completely independent and do not interfere with each other; if all four ball valves 301 are open, the two air pipes 3 in each observation room complete the allelochemical exchange of gases through the exchange chamber 4. Further, by sequentially closing one air pipe 3 on each side of the top and bottom surfaces of the exchange chamber 4, the remaining air pipes 3 in the two observation rooms are positioned such that one is on the top surface of the exchange chamber 4 and the other on the bottom surface, thus forming an interconnection. This is coordinated with the motor for forward and reverse rotation. If the air pipe 3 on the left side of the exchange chamber is on its top surface and the air pipe 3 on the right side is on its bottom surface, then the fan 401 rotates forward, as shown in the instruction manual. Figure 2As shown, the fan 401 compresses the gas behind it, which is the gas drawn from the left-side air pipe 3. This corresponds to the gas in the left observation room being drawn into the exchange chamber 4. Then, the space in front of the fan, i.e., the right-side air pipe 3, is powered by the air output from the fan 401, carrying the gas from the left observation room into the right observation room, thus completing the exchange of allelochemicals. Furthermore, if the gas in the right observation room is to be introduced into the left observation room, the reverse motor drives the fan 401 to reverse, or one air pipe 3 on the top and bottom surfaces of both sides of the exchange chamber 4 is closed in sequence, so that the air pipe 3 on the left side of the exchange chamber is on its bottom surface and the air pipe 3 on the right side is on its top surface, the forward motor continues to drive the fan 401 to rotate forward, thus conducting an allelochemical mixing observation experiment on the plants in the two observation rooms.

[0044] In use, after two plants are placed in incubator 1, the external laboratory lights can be turned on to supplement the light and promote photosynthesis. Furthermore, as the plants produce oxygen through photosynthesis, this oxygen contains allelochemicals. At this point, the allelochemicals can be collected. The air bladder 6, connected to the branch pipe 502 via the three-way valve 501, is used to collect the allelochemicals from the two observation chambers. All ball valves 301 on the four air pipes 3 are closed to lock them. To collect the allelochemicals of the plant at the current growth stage in the observation chamber, the ball valve 301 of the air pipe 3 in the observation chamber without the branch pipe 502 is opened, and then the three-way valve 501 of the other observation chamber with the branch pipe 502 is opened, ensuring that the airflow only flows into the air bladder 6. At this point, the front and rear ends of the branch pipe 502 are indirectly connected to one observation chamber and one air bladder 6, respectively. Then, the motor is started to drive the fan 401 to rotate. It is worth noting that the fan rotation... The direction of movement must ensure that the air tube 3 without branch pipe 502 is connected to the rear. At this time, the fan 401 forms a pump-like effect between the observation room and the airbag 6, drawing the allelochemical from the observation room into the inside of the airbag 6. When the airbag 6 collects the allelochemical from the observation room, the airflow passes through the filter 602. The elution layer on the surface of the filter 602 adsorbs the allelochemical in the airflow. The collected airbag 6 can be either disassembled or injected into another observation room. If disassembly is chosen, the airbag 6 is rotated in the opposite direction so that the screw ring 601 at its front end moves relative to the screw sleeve 503, causing the screw ring 601 to detach from the screw sleeve 503. At this time, the filter 602 inside the screw ring 601 is exposed. The filter 602 has adsorbed the allelochemical from the observation room and can be directly removed for the next step of research. The removed filter 602 is heated by electricity to release the gas containing the allelochemical and then collected separately. The collected gas containing the allelochemical is then sent back into the observation room.

[0045] Example 2 In this embodiment, the exchange chamber 4 is an internally hollow cylinder parallel to the top surface of the incubator 1. The incubator 1 is made of tempered glass or acrylic sheet, is bidirectionally transparent, and is bonded with glass glue. Not shown in the accompanying drawings, the outer surface of the incubator 1 is also fitted with reinforcing ribs. Since adding reinforcing ribs to the glass surface for explosion protection is a common technical solution and not an improvement, it will not be elaborated further. In this embodiment, the elution layer on the surface of the filter 602 is Tenax-T or Tenax-GR, with a thickness of 0.1 mm. After the two plants to be subjected to allelopathic experiments are placed in the incubator 1, the external laboratory lights can be turned on to supplement the light and promote photosynthesis. Furthermore, as the plants produce oxygen through photosynthesis, this oxygen contains allelopathic substances from the plants. At this point, the allelopathic substances can be collected and processed through exchange... The front and rear ends of the flow chamber 4 are sealed and connected to the four air pipes 3 extending from the two observation rooms, forming an interconnection between the two observation rooms. Combined with the aforementioned technical solution, each air pipe 3 is equipped with a ball valve 301 at its bottom. This allows for various combinations: if all four ball valves 301 are closed, the two observation rooms become completely independent and do not interfere with each other; if all four ball valves 301 are open, the two air pipes 3 in each observation room complete the chemiluminescent exchange of gases through the exchange chamber 4. Furthermore, by sequentially closing one air pipe 3 at the front and one at the rear of each side of the exchange chamber 4, the remaining air pipes 3 in the two observation rooms are positioned such that one is at the front of the exchange chamber 4 and the other at the rear, thus forming an interconnection. This, combined with the motor's forward and reverse rotation, allows the fan 401 to rotate forward if the air pipe 3 on the left side of the exchange chamber is at its front and the air pipe 3 on the right side is at its rear. (See the attached instruction manual.) Figure 2 As shown, the fan 401 compresses the gas behind it, which is the gas drawn from the right-side air pipe 3 at the rear end. This corresponds to the gas in the right observation room being drawn into the exchange chamber 4. Then, the space in front of the fan, i.e., the air pipe 3 on the left side, is powered by the air output from the fan 401, carrying the gas from the right observation room into the left observation room, thus completing the exchange of allelochemical substances. Further, if the gas in the left observation room is to be introduced into the right observation room, the reverse motor drives the fan 401 to reverse, or one air pipe 3 at the front end and one air pipe 3 at the rear end on both sides of the exchange chamber 4 are closed in sequence, so that the air pipe 3 on the left side of the exchange chamber is at its rear end and the air pipe 3 on the right side is at its front end, the forward motor continues to drive the fan 401 to rotate forward.

[0046] Furthermore, this embodiment can also release the collected allelochemicals. First, rotate the airbag 6 in the opposite direction so that the screw ring 601 at its front end moves relative to the screw sleeve 503, causing the screw ring 601 to disengage from the screw sleeve 503. At this time, the filter 602 inside the screw ring 601 is exposed. The filter 602 of the airbag 6 is removed separately. Repeat the aforementioned technical solution to reinstall the airbag 6 into the branch pipe 502. Close all the ball valves 301 of the four air pipes 3 to lock the air pipes 3. Then, to collect allelochemicals of the current growth stage of the plants in the observation room, open the ball valve 301 of the air pipe 3 in the observation room where the allelochemicals need to be collected that does not have the branch pipe 502 installed. Then open the three-way valve of the other observation room where the branch pipe 502 is installed. 501, ensuring that the airflow direction can only flow into the airbag 6. In embodiment 1, the airflow direction of the exchange chamber 4 is longitudinal, while in this embodiment, the airflow direction is transverse. Start the motor to drive the fan 401 to rotate. It is worth noting that the direction of the fan rotation must ensure that the air pipe 3 connected to the rear is not connected to the branch pipe 502. At this time, the fan 401 forms a pump-like effect between the observation room and the airbag 6, drawing the allelochemicals from the observation room into the inside of the airbag 6. Then, close the ball valve 301 of the air pipe 3 in the observation room that is not connected to the branch pipe 502, and open the ball valve 301 of the air pipe 3 in another observation room that is not connected to the branch pipe 502. At this time, the plant allelochemicals collected in the observation room enter the other observation room.

[0047] Furthermore, in this embodiment, the three-way valve 501 is kept in the normally closed state of the air chamber 6 by default. In experiments, it is not only necessary to collect allelochemicals, but sometimes it is also necessary to mix allelochemicals from two observation rooms to observe plant growth. The four air pipes 3 extending from the two observation rooms are sealed and connected to the front and rear ends of the two sides of the exchange chamber 4, forming an interconnection between the two observation rooms. Combined with the aforementioned technical solution, a ball valve 301 is installed at the bottom of each air pipe 3. This allows for various combinations. For example, if all four ball valves 301 are closed, the two observation rooms become completely independent and do not interfere with each other. Alternatively, if all four ball valves 301 are open, the two air pipes 3 of each observation room complete the allelochemical exchange of gases through the exchange chamber 4. Further, by sequentially closing one air pipe 3 at the front and one at the rear of each side of the exchange chamber 4, the remaining air pipes 3 of the two observation rooms are positioned such that one is at the front end of the exchange chamber 4 and the other at the rear end, thus forming an interconnection. This is coordinated with the motor for forward and reverse rotation. If the air pipe 3 on the left side of the exchange chamber is at its front end and the air pipe 3 on the right side is at its rear end, then the fan 401 rotates forward, as shown in the attached instruction manual. Figure 2As shown, the fan 401 compresses the gas behind it, which is the gas drawn from the right-side air pipe 3 at the rear end. This corresponds to the gas in the right observation room being drawn into the exchange chamber 4. Then, the space in front of the fan, i.e., the air pipe 3 on the left side, is powered by the air output from the fan 401, carrying the gas from the right observation room into the left observation room, thus completing the exchange of allelochemicals. Furthermore, if the gas in the left observation room is to be introduced into the right observation room, the reverse motor drives the fan 401 to reverse, or one air pipe 3 at the front end and one air pipe 3 at the rear end on both sides of the exchange chamber 4 are closed in sequence, so that the air pipe 3 on the left side of the exchange chamber is at its rear end and the air pipe 3 on the right side is at its front end, the forward motor continues to drive the fan 401 to rotate forward, thus conducting an allelochemical mixing observation experiment on the plants in the two observation rooms.

[0048] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. An apparatus for studying allelopathy among plants, comprising a culture chamber (1), wherein a partition plate (101) is vertically mounted on the middle of the inner side of the culture chamber (1), the partition plate (101) and the four sides of the culture chamber (1) are sealed, and the inner side of the culture chamber (1) is separated by the partition plate (101) into two observation rooms for placing plants, characterized in that: Each of the two observation rooms has an air hole (2) that penetrates the top surface of the incubator (1) on its inner side. Each observation room has at least two air holes (2). The top of the air hole (2) is open and located on the top surface of the incubator (1). An air pipe (3) is connected to the air pipe (3) through a flange. A ball valve (301) is movably installed at the bottom of the air pipe (3). The top of the air pipe (3) extends toward the top of the incubator (1) and the tail end is connected to the communication chamber (4). The communication chamber (4) is equipped with an communication structure for allelopathic communication between the two observation rooms. Includes a fan (401), which is located in the middle of the inner side of the communication chamber (4). The fan (401) has two observation rooms on its left and right sides, and the fan (401) is connected to the air pipes (3) extending from the two observation rooms at the front and rear. It also includes an extension tube (5), which is installed at the end of the air tube (3) and inserted into the inside of the communication chamber (4). At least one of the two air tubes (3) extending from each observation room has an extension tube (5) installed at its end. A three-way valve (501) is installed in the middle of the extension tube (5). The two ends of the three-way valve (501) are respectively connected to the extension tube (5) and the last end is movably connected to the airbag (6). The three-way valve (501) is connected to one end of the airbag (6) and also includes a branch pipe (502). The branch pipe (502) is a hollow pipe with a fixed connection to the tail end of the branch pipe (502). The screw sleeve (503) is also hollow and its tail end forms a sealed connection with the tail end of the branch pipe (502). The tail end of the screw sleeve (503) is threadedly connected to a screw ring (601) fixedly installed on the front wall of the airbag (6). The inner side of the screw ring (601) is also hollow and its tail end is fixedly connected to the opening of the front wall of the airbag (6). The outer surface of the screw ring (601) is threadedly connected to the inner side of the screw sleeve (503). A filter (602) is inserted into the inner side of the screw ring (601). The filter (602) surface is also coated with an elution layer.

2. The apparatus for studying allelopathy among plants according to claim 1, characterized in that: The fan (401) is also fixedly connected to the output end of a motor, which is mounted on the outer surface of the AC compartment (4).

3. The apparatus for studying allelopathy among plants according to claim 1 or 2, characterized in that: The incubator (1) is made of tempered glass or acrylic sheet, and is transparent in both directions, and is bonded with glass glue.

4. The apparatus for studying allelopathy among plants according to claim 3, characterized in that: The eluent layer is one of Tenax-TA, Tenax-GR, activated carbon, and silica gel, and the thickness of the eluent layer is 0.1 mm.

5. The apparatus for studying allelopathy among plants according to claim 4, characterized in that: The communication chamber (4) is an internal hollow cylinder perpendicular to the top surface of the incubator (1). The top and bottom surfaces on both sides of the communication chamber (4) are also sealed and connected to four air tubes (3) extending from the two observation rooms.

6. The apparatus for studying allelopathy among plants according to claim 5, characterized in that: The communication structure also includes four air pipes (3) extending from the two observation rooms to support the communication chamber (4). One air pipe (3) extending from a single observation room is connected to the bottom surface of the communication chamber (4) to form a vertical shape, and the other air pipe (3) is connected to the top surface of the communication chamber (4) to form an inverted "L" shape. The four air pipes (3) and the communication chamber (4) together form an M-shaped support for the communication chamber (4).

7. The apparatus for studying allelopathy among plants according to claim 4, characterized in that: The communication chamber (4) is an internal hollow cylinder parallel to the top surface of the incubator (1). The front and rear ends of the two sides of the communication chamber (4) are also sealed and connected to four air tubes (3) extending from the two observation rooms. The tail ends of the four air tubes (3) are all connected to extension tubes (5), but only one of the two air tubes (3) extending from each observation room has an air bag (6) installed on the extension tube (5) at the tail end of the air tube (3).

8. The apparatus for studying allelopathy among plants according to claim 7, characterized in that: The communication structure also includes a support bracket (7), the top of which is an arc shape that fits the bottom surface of the communication compartment (4), and the bottom of which is vertically connected to the top surface of the communication compartment (4).

9. The apparatus for studying allelopathy among plants according to claim 8, characterized in that: The fan (401) is located at the center of the inner side of the communication chamber (4) parallel to the top surface of the incubator (1), and each of the front and rear ends of the fan (401) has an extension tube (5) with an opening inside the communication chamber (4).

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