A plant oil-control anti-hair loss composition, and a preparation method and application thereof

Abstract

The application belongs to the technical field of cosmetics, and particularly relates to a plant oil-controlling and hair-loss-preventing composition as well as a preparation method and application thereof. The preparation method comprises the following steps: cleaning, airing, crushing and sterilizing mixed raw materials of Radix Polygoni Multiflori, Ligustri Lucidi Fructus, Tribulus terrestris and Panax ginseng to obtain pretreated solid-state fermentation raw materials; adding Aspergillus oryzae suspension and Monascus suspension into the pretreated solid-state fermentation raw materials in sequence for step-by-step fermentation culture; extracting the fermented raw materials with an ethanol aqueous solution, concentrating the extract, and redissolving the concentrated extract with pure water to obtain A liquid; and using the A liquid as an extraction solvent to heat-extract mixed raw materials of Platycladus orientalis, Crocus sativus, Lithospermum erythrorhizon, Chuanxiong and Sophora flavescens, so as to obtain the plant oil-controlling and hair-loss-preventing composition. The composition obtained by the application has significantly improved oil-controlling effect and hair-loss-preventing effect.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, specifically relating to a plant-based oil-controlling and hair loss-preventing composition, its preparation method, and its application. Background Technology

[0002] Currently, the mainstream treatments for hair loss include medication, hair transplantation surgery, low-energy laser therapy (LLLT), and hair regrowth technology. Among medications, minoxidil and finasteride are FDA-approved for treating hair loss. These medications have side effects such as headaches, anxiety, hirsutism, irritation, contact dermatitis, memory loss, and decreased libido. Minoxidil has been reported to have teratogenic effects on fetuses, therefore it is not suitable for pregnant or breastfeeding women, and hair loss often recurs after discontinuation, requiring long-term continuous treatment. Hair transplantation is invasive and expensive, with drawbacks including long surgery time, postoperative pain, and infection. Low-energy laser therapy stimulates hair follicles in the telogen and catagen phases to transition to the anagen phase, delaying the growth phase and increasing the number of follicles in the anagen phase. It has some effect in mild to moderate AGA patients, but has side effects such as edema, erythema, dandruff, local pain, itching, erosion, and hair shaft damage.

[0003] Ancient texts contain numerous records and case studies of using plant-based ingredients to alleviate / treat hair loss, such as ginger, arborvitae leaves, and Polygonum multiflorum. Compared to the aforementioned treatments, plant-based ingredients are gentler, safer, and have a multi-target effect, simultaneously providing treatment, maintenance, and consolidation. With technological advancements, contemporary methods primarily utilize functional extracts of these plant-based ingredients to address the corresponding problems. For example, patent document CN111904882A describes a composition with scalp oil control, dandruff removal, mite removal, and hair loss prevention properties. This composition includes water and extracts of arborvitae leaves, Polygonum multiflorum, tea seed, purslane, angelica, ganoderma, eclipta, sophora flavescens, chuanxiong, atractylodes, artemisia, cnidium, honeysuckle, ginger, clematis, mulberry, and cordyceps. Patent document CN119770373A discloses an anti-hair loss, oil-controlling, and antibacterial composition, as well as a shampoo and conditioner. This anti-hair loss, oil-controlling, and antibacterial composition comprises type XVII collagen, hydrolyzed keratin, Platycladus orientalis leaf extract, Sophora flavescens extract, Forsythia suspensa extract, and Artemisia argyi extract in a mass ratio of 0.5:0.5:3:2:1:1.

[0004] However, simple combinations of plant extracts often have limited effects. Enhancing the efficacy of specific plant extracts through special processes and applying them to cosmetic products has broad market prospects. Summary of the Invention

[0005] In view of the shortcomings and deficiencies of the existing technology, the primary objective of this invention is to provide a method for preparing a plant-based oil-controlling and hair loss-preventing composition.

[0006] Another object of the present invention is to provide a plant-based oil-controlling and hair loss-preventing composition prepared by the above method.

[0007] Another object of the present invention is to provide the application of the above-mentioned plant-based oil-controlling and hair loss-preventing composition in shampoo and conditioner products.

[0008] The objective of this invention is achieved through the following technical solution:

[0009] A method for preparing a plant-based oil-controlling and hair loss-preventing composition includes the following preparation steps:

[0010] (1) The mixed raw materials of processed Polygonum multiflorum, Ligustrum lucidum, Tribulus terrestris and ginseng are washed, dried, crushed and sterilized to obtain pretreated solid fermentation raw materials;

[0011] (2) Add Aspergillus oryzae suspension to the pretreated solid fermentation raw material in step (1), stir and mix well, and ferment at 32-35℃ for 48-96h with sterile air. Then add Monascus purpureus suspension, stir and mix well, and ferment at 30-32℃ for 48-96h with sterile air before terminating fermentation.

[0012] (3) Extract the raw material after fermentation in step (2) with an ethanol aqueous solution, concentrate the extract to a thin paste, and redissolve the concentrate with pure water to obtain solution A;

[0013] (4) Using the A solution obtained in step (3) as the extraction solvent, the mixed raw materials of Platycladus orientalis leaves, saffron, gromwell root, chuanxiong rhizome and sophora flavescens were heated and extracted. The resulting extract is the plant oil-controlling and hair loss-preventing composition.

[0014] Further, the mass ratio of processed Polygonum multiflorum, Ligustrum lucidum, Tribulus terrestris and ginseng in step (1) is as follows: processed Polygonum multiflorum 10-20 parts, Ligustrum lucidum 10-20 parts, Tribulus terrestris 2-10 parts, and ginseng 2-10 parts.

[0015] Furthermore, the sterilization in step (1) is performed at 121°C for 30 minutes.

[0016] Further, the Aspergillus oryzae suspension described in step (2) is prepared by the following method:

[0017] Aspergillus oryzae was inoculated into PDA medium to activate the strain, and then transferred to modified Czapek's liquid medium for culture until the bacterial concentration reached 1×10⁻⁶. 8 A suspension of Aspergillus oryzae was obtained with a concentration of cfu / mL or higher.

[0018] More preferably, the liquid-to-solid ratio of the Aspergillus oryzae suspension is 5-15% (V / W). That is, 50-150 ml of the suspension is added to 1 kg of pretreated solid-state fermentation raw material. More preferably, the liquid-to-solid ratio is 10%.

[0019] Furthermore, the Monascus purpureus suspension described in step (2) is prepared by the following method:

[0020] Monascus purpureus was inoculated into PDA medium to activate the strains, and then transferred to esterase liquid medium for culture until the bacterial concentration reached 1×10⁻⁶. 8 A suspension of Monascus purpureus was obtained with a concentration of cfu / mL or higher.

[0021] More preferably, the liquid-to-solid ratio of the Monascus purpureus suspension is 2-10% (V / W). More preferably, the liquid-to-solid ratio is 5%.

[0022] Further, in step (3), the volume content of ethanol in the aqueous ethanol solution is 60-80%; the material-to-liquid ratio of the aqueous ethanol solution is 1:5-15 (W / V), the extraction temperature is 60-80℃, and the extraction time is 1-3 h.

[0023] Furthermore, the amount of pure water used for redissolving in step (3) is 5-15 times the amount of the concentrated substance.

[0024] Furthermore, the mass fraction ratio of Platycladus orientalis leaves, saffron, gromwell root, chuanxiong rhizome, and sophora flavescens in step (4) is as follows:

[0025] 2-10 parts of arborvitae leaves, 2-10 parts of saffron, 5-15 parts of gromwell root, 2-10 parts of chuanxiong rhizome, and 5-15 parts of sophora root.

[0026] Furthermore, the heating extraction temperature in step (4) is 80-90℃, and the time is 1-3h.

[0027] Further, after the heating extraction described in step (4), the supernatant is further filtered and centrifuged; then the concentration of the extract is adjusted to 0.1-0.2 g / g by replenishing the weight with pure water or by heating and concentrating.

[0028] A plant-based oil-controlling and hair loss-preventing composition was prepared by the above method.

[0029] Application of the above-mentioned plant-based oil-controlling and hair loss-preventing compositions in shampoo and conditioner products.

[0030] Compared with the prior art, the beneficial effects of the present invention are:

[0031] (1) In this invention, Aspergillus oryzae and Monascus purpureus are used to ferment the raw materials of Polygonum multiflorum, Ligustrum lucidum, Tribulus terrestris and ginseng through a specific process, and the fermented extract components are obtained through a specific extraction process. It has been verified that the oil control effect and hair loss prevention effect of the obtained composition can be significantly improved.

[0032] (2) The present invention uses a solvent containing specific fermentation extract components to heat extract a mixture of raw materials including arborvitae leaves, saffron, gromwell root, chuanxiong rhizome and sophora flavescens. It has been verified that this can significantly improve the oil control effect and hair loss prevention effect of the obtained composition. Detailed Implementation

[0033] The present invention will be further described in detail below with reference to embodiments, but the implementation of the present invention is not limited thereto.

[0034] The Aspergillus oryzae and Monascus purpureus suspensions used in the following examples were prepared by the following methods:

[0035] Aspergillus oryzae (CICC 2386, purchased from the China Industrial Microbial Culture Collection Center) was inoculated onto PDA medium and cultured at 37°C for 24 h to activate the strain. Then, it was transferred to modified Czapek's liquid medium (sucrose 30 g / L, NaNO3 2 g / L, K2HPO4 1 g / L, MgSO4·7H2O 0.5 g / L, FeSO4 0.01 g / L) and cultured in shake flasks for 24 h until the bacterial concentration reached 5 × 10⁻⁶. 8 The concentration of cfu / mL was increased to obtain a suspension of Aspergillus oryzae for storage.

[0036] Monascus purpureus (CGMCC 41681, purchased from China Industrial Microbial Culture Collection Center) was inoculated onto PDA medium and cultured at 37°C for 24 h to activate the strain. Then, it was transferred to a high-yield esterase liquid medium (soluble starch 70 g / L, peptone 25 g / L, MgSO4·7H2O 1 g / L) and cultured in shake flasks for 24 h until the bacterial concentration reached 5 × 10⁻⁶. 8 The concentration of cfu / mL was increased to obtain a suspension of Monascus purpureus for storage and future use.

[0037] Example 1

[0038] A method for preparing a plant-based oil-controlling and hair loss-preventing composition includes the following preparation steps:

[0039] (1) Take 15g of processed Polygonum multiflorum, 15g of Ligustrum lucidum, 5g of Tribulus terrestris and 5g of ginseng as mixed raw materials, wash, dry, crush, sterilize at 121℃ for 30min to obtain pretreated solid fermentation raw materials.

[0040] (2) Add Aspergillus oryzae suspension to the pretreated solid fermentation raw material in step (1) and stir well. The liquid-to-solid ratio is 10% (V / W). Ferment with sterile air and culture at 32-35℃ for 72h. Then add Monascus purpureus suspension and stir well. The liquid-to-solid ratio is 5% (V / W). Ferment with sterile air and culture at 30-32℃ for 72h before terminating fermentation.

[0041] (3) Extract the raw material after fermentation in step (2) with an 80% ethanol aqueous solution at a material-to-liquid ratio of 1:10 (W / V), at an extraction temperature of 70℃ and an extraction time of 2h. Concentrate the extract to a thin paste and redissolve the concentrate with 10 times its weight of pure water to obtain solution A.

[0042] (4) Take 5g of arborvitae leaves, 5g of saffron, 10g of gromwell root, 5g of chuanxiong rhizome and 10g of sophora flavescens mixed raw materials, use the A solution obtained in step (3) as the extraction solvent for heating extraction, the extraction temperature is 85℃ and the extraction time is 2h. After extraction, filter, centrifuge and take the supernatant, then add pure water to make up the weight or heat to concentrate to adjust the concentration of the raw drug in the supernatant to 0.15g / g, and the plant oil-controlling and hair loss prevention composition is obtained.

[0043] Example 2

[0044] A method for preparing a plant-based oil-controlling and hair loss-preventing composition includes the following preparation steps:

[0045] (1) Take 10g of processed Polygonum multiflorum, 10g of Ligustrum lucidum, 2g of Tribulus terrestris and 2g of ginseng as mixed raw materials, wash, dry, crush, sterilize at 121℃ for 30min to obtain pretreated solid fermentation raw materials.

[0046] (2) Add Aspergillus oryzae suspension to the pretreated solid fermentation raw material in step (1) and stir well. The liquid-to-solid ratio is 10% (V / W). Ferment with sterile air and culture at 32-35℃ for 72h. Then add Monascus purpureus suspension and stir well. The liquid-to-solid ratio is 5% (V / W). Ferment with sterile air and culture at 30-32℃ for 72h before terminating fermentation.

[0047] (3) Extract the raw material after fermentation in step (2) with 60% ethanol aqueous solution at a material-to-liquid ratio of 1:10 (W / V), at an extraction temperature of 70℃ and an extraction time of 2h. Concentrate the extract to a thin paste and redissolve the concentrate with 10 times its weight of pure water to obtain solution A.

[0048] (4) Take 10g of arborvitae leaves, 10g of saffron, 15g of gromwell root, 10g of chuanxiong rhizome and 15g of sophora flavescens mixed raw materials, use the A solution obtained in step (3) as the extraction solvent for heating extraction, the extraction temperature is 85℃ and the extraction time is 2h. After extraction, filter, centrifuge and take the supernatant, then add pure water to make up the weight or heat to concentrate to adjust the concentration of the raw drug in the supernatant to 0.15g / g, and the plant oil-controlling and hair loss prevention composition is obtained.

[0049] Example 3

[0050] A method for preparing a plant-based oil-controlling and hair loss-preventing composition includes the following preparation steps:

[0051] (1) Take 20g of processed Polygonum multiflorum, 20g of Ligustrum lucidum, 10g of Tribulus terrestris and 10g of ginseng as mixed raw materials, wash, dry, crush, sterilize at 121℃ for 30min to obtain pretreated solid fermentation raw materials.

[0052] (2) Add Aspergillus oryzae suspension to the pretreated solid fermentation raw material in step (1) and stir well. The liquid-to-solid ratio is 10% (V / W). Ferment with sterile air and culture at 32-35℃ for 72h. Then add Monascus purpureus suspension and stir well. The liquid-to-solid ratio is 5% (V / W). Ferment with sterile air and culture at 30-32℃ for 72h before terminating fermentation.

[0053] (3) Extract the raw material after fermentation in step (2) with an 80% ethanol aqueous solution at a material-to-liquid ratio of 1:10 (W / V), at an extraction temperature of 70℃ and an extraction time of 2h. Concentrate the extract to a thin paste and redissolve the concentrate with 10 times its weight of pure water to obtain solution A.

[0054] (4) Take 2g of arborvitae leaves, 2g of saffron, 5g of gromwell root, 2g of chuanxiong rhizome and 5g of sophora flavescens mixed raw materials, use the A solution obtained in step (3) as the extraction solvent for heating extraction, the extraction temperature is 85℃ and the extraction time is 2h. After extraction, filter, centrifuge and take the supernatant, then add pure water to make up the weight or heat to concentrate to adjust the concentration of the raw drug in the supernatant to 0.15g / g, and the plant oil-controlling and hair loss prevention composition is obtained.

[0055] Comparative Example 1

[0056] A method for preparing a plant-based oil-controlling and hair loss-preventing composition, which differs from Example 1 in that step (2) uses separate Aspergillus oryzae fermentation, the specific process of which is as follows:

[0057] Add Aspergillus oryzae suspension to the pretreated solid fermentation raw material in step (1) and stir to mix. The liquid-to-solid ratio is 15% (V / W). Fermentation is carried out under sterile air and the fermentation is terminated after being cultured at 32-35℃ for 72 hours.

[0058] Comparative Example 2

[0059] A method for preparing a plant-based oil-controlling and hair loss-preventing composition, which differs from Example 1 in that step (2) uses separate Monascus purpureus fermentation, the specific process of which is as follows:

[0060] Add Monascus purpureus suspension to the pretreated solid fermentation raw material in step (1) and stir to mix. The liquid-to-solid ratio is 15% (V / W). Fermentation is carried out under sterile air and the fermentation is terminated after being cultured at 30-32℃ for 72 hours.

[0061] Comparative Example 3

[0062] A method for preparing a plant-based oil-controlling and hair loss-preventing composition, which differs from Example 1 in that step (2) involves simultaneous fermentation with Aspergillus oryzae and Monascus purpureus, the specific process of which is as follows:

[0063] In step (1), Aspergillus oryzae suspension and Monascus purpureus suspension were added to the pretreated solid fermentation raw materials and stirred until well mixed. The liquid-to-solid ratio of Aspergillus oryzae suspension was 10% (V / W) and the liquid-to-solid ratio of Monascus purpureus suspension was 5% (V / W). Fermentation was carried out under sterile air and the fermentation was terminated after being cultured at 32±2℃ for 72 hours.

[0064] Comparative Example 4

[0065] A method for preparing a plant-based oil-controlling and hair loss-preventing composition, which differs from Example 1 in that solution A uses a liquid fermentation broth of Aspergillus oryzae + Monascus purpureus, and the specific process is as follows:

[0066] The pretreated solid-state fermentation raw material from step (1) was mixed with water at a mass ratio of 1:15 to obtain liquid fermentation raw material. Then, Aspergillus oryzae suspension was added and stirred until homogeneous. The liquid-to-material ratio of Aspergillus oryzae suspension to pretreated solid-state fermentation raw material was 10% (V / W). Fermentation was carried out under sterile air at 32-35℃ for 72 hours. Then, Monascus purpureus suspension was added and stirred until homogeneous. The liquid-to-material ratio of Monascus purpureus suspension to pretreated solid-state fermentation raw material was 5% (V / W). Fermentation was carried out under sterile air at 30-32℃ for 72 hours, after which fermentation was terminated. The fermentation broth was centrifuged at 8000 rpm for 6 minutes, and the supernatant was collected and sterilized at 100℃ for 30 minutes to obtain solution A.

[0067] Comparative Example 5

[0068] A method for preparing a plant-based oil-controlling and hair loss-preventing composition, compared with Example 1, differs in that step (3) uses pure water instead of 80 wt% ethanol aqueous solution to extract the raw materials after fermentation in step (2). The specific process is as follows:

[0069] Extract the raw material after fermentation in step (2) with pure water at a ratio of 1:10 (W / V), at a temperature of 70℃, for 2 hours. Filter the extract, centrifuge and collect the supernatant to obtain liquid A.

[0070] Comparative Example 6

[0071] A method for preparing a plant-based oil-controlling and hair loss-preventing composition, compared with Example 1, differs in that step (4) uses pure water instead of solution A for heating extraction. The specific process is as follows:

[0072] Take a mixture of 5g of arborvitae leaves, 5g of saffron, 10g of gromwell root, 5g of chuanxiong rhizome and 10g of sophora root, and use 10 times the mass of pure water for heating and extraction. The extraction temperature is 85℃ and the extraction time is 2h. After extraction, filter and centrifuge to obtain the supernatant. Then mix it with liquid A obtained in step (3). Adjust the concentration of the raw herbs in the supernatant to 0.15g / g by adding pure water to make up the weight or by heating and concentrating. The plant oil-controlling and hair loss prevention composition is obtained.

[0073] Comparative Example 7

[0074] A method for preparing a plant-based oil-controlling and hair loss-preventing composition, compared with Example 1, differs in that liquid A is obtained by fermentation of pure water extracts of Polygonum multiflorum, Ligustrum lucidum, Tribulus terrestris, and ginseng. The specific process is as follows:

[0075] (1) Take 15g of processed Polygonum multiflorum, 15g of Ligustrum lucidum, 5g of Tribulus terrestris and 5g of ginseng as mixed raw materials, wash, dry, crush, sterilize at 121℃ for 30min, and extract the sterilized raw materials by reflux with pure water at a material-to-liquid ratio of 1:15 for 2h. Centrifuge at 8000rpm*6min to obtain the supernatant and obtain the water extract.

[0076] (2) Add Aspergillus oryzae suspension to the water extract from step (1) and stir well. The liquid-to-solid ratio of Aspergillus oryzae suspension to the sterilized raw material is 10% (V / W). Fermentation is carried out under sterile air at 32-35℃ for 72 hours. Then add Monascus purpureus suspension and stir well. The liquid-to-solid ratio of Monascus purpureus suspension to the sterilized raw material is 5% (V / W). Fermentation is carried out under sterile air at 30-32℃ for 72 hours, and then the fermentation is terminated. Centrifuge the fermentation broth at 8000 rpm for 6 minutes and collect the supernatant. Sterilize at 100℃ for 30 minutes to obtain solution A.

[0077] Comparative Example 8

[0078] A method for preparing a plant-based oil-controlling and hair loss-preventing composition, compared with Example 1, differs in that liquid A is obtained by fermentation of ethanol-water extracts of Polygonum multiflorum, Ligustrum lucidum, Tribulus terrestris, and ginseng. The specific process is as follows:

[0079] (1) Take 15g of processed Polygonum multiflorum, 15g of Ligustrum lucidum, 5g of Tribulus terrestris and 5g of ginseng as mixed raw materials, wash, dry, crush, sterilize at 121℃ for 30min, extract the sterilized raw materials with 80vt% ethanol aqueous solution at a material-liquid ratio of 1:15 for 2h by reflux, centrifuge at 8000rpm*6min to collect the supernatant, concentrate the extract to a thin paste, and redissolve in pure water to the initial concentration to obtain ethanol aqueous extract solution.

[0080] (2) Add Aspergillus oryzae suspension to the ethanol-water extract solution from step (1) and stir well. The liquid-to-solid ratio of Aspergillus oryzae suspension to the sterilized raw material is 10% (V / W). Fermentation is carried out under sterile air at 32-35℃ for 72 hours. Then add Monascus purpureus suspension and stir well. The liquid-to-solid ratio of Monascus purpureus suspension to the sterilized raw material is 5% (V / W). Fermentation is carried out under sterile air at 30-32℃ for 72 hours, and then the fermentation is terminated. Centrifuge the fermentation broth at 8000 rpm for 6 minutes and collect the supernatant. Sterilize at 100℃ for 30 minutes to obtain solution A.

[0081] The oil control and hair loss prevention effects of the compositions obtained in the above embodiments and comparative examples were tested:

[0082] I. Oil Control Effect Test

[0083] Oil control refers to the ability to reduce sebum secretion and deposition at the application site, or to minimize oiliness. People with oily skin have overactive sebaceous glands and high lipid synthesis, resulting in a shiny skin surface. Oil Red O is a fat-soluble dye, highly soluble in fats, and specifically stains neutral fats within tissues. It has poor staining properties on other cellular structures. Its staining principle is based on physical solution action or adsorption, using solution action to stain fats. By detecting the characteristics of Oil Red O, the amount of lipid synthesis can be assessed to evaluate the effect of the test sample on lipid synthesis in SZ95 human sebaceous gland cells. The lower the amount of lipid synthesis, the better the oil control performance of the cosmetic.

[0084] The test procedure was as follows: SZ95 human sebaceous gland cells were seeded into well plates and cultured for 24 h → samples were weighed → the test samples were pretreated to prepare a mixed solution (with a blank control group) → placed into the wells and incubated at 37℃ for 48 h → stained with Oil Red O, dried and examined under a microscope → data were analyzed, and lipid synthesis and significance P-values ​​were calculated. The test results are shown in Table 1 below:

[0085] Table 1. Oil control effect test results of the compositions of Examples 1-3 and Comparative Examples 1-8

[0086] z

[0087] II. Hair loss prevention effect testing for actual products

[0088] Using the anti-hair loss and oil-control essence formula shown in Table 2, human trials of anti-hair loss cosmetics were conducted in accordance with the "Cosmetic Safety Technical Specifications" (2015 edition).

[0089] Subjects were screened and enrolled (healthy men or women aged 20-60 years who met the inclusion criteria). Eligible subjects were divided into groups of 10 based on the test samples. During the test, each group of subjects used the "Anti-Hair Loss and Oil Control Essence" of this invention at a dosage of 3-5 ml per application for 10 weeks. No other products of the same type were allowed to be used during the trial.

[0090] Hair loss was counted using the 50-comb method before and 10 weeks after the test, and local hair density (unit: roots / cm²) was measured using an image acquisition device. 2 ).

[0091] Hair loss measurement change rate (%) = (hair loss before test - hair loss after 10 weeks) / hair loss before test × 100%.

[0092] Local hair density change rate = (local hair density after 10 weeks - local hair density before test) / local hair density before test × 100%.

[0093] The test results are shown in Table 3 below.

[0094] Table 2. Formula Table for Anti-Hair Loss Efficacy Testing

[0095]

[0096] Table 3. Test results of the anti-hair loss efficacy of the formulations of Examples 1-3 and Comparative Examples 1-8

[0097]

[0098] The results above show that the present invention uses Aspergillus oryzae and Monascus purpureus to carry out stepwise solid-state fermentation of a mixture of processed Polygonum multiflorum, Ligustrum lucidum, Tribulus terrestris and ginseng, and extracts the solid-state fermentation product with an ethanol-water solution. Then, the pure aqueous solution of the above extract is used as the extraction solvent to heat-extract a mixture of Platycladus orientalis leaves, saffron, Lithospermum erythrorhizon, Ligusticum chuanxiong and Sophora flavescens. The composition prepared by the above specific process has significantly improved oil control and hair loss prevention effects.

[0099] The above embodiments are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the above embodiments. Any changes, modifications, substitutions, combinations, or simplifications made without departing from the spirit and principle of the present invention shall be considered equivalent substitutions and shall be included within the protection scope of the present invention.

Claims

1. A method for preparing a plant-based oil-controlling and hair loss-preventing composition, characterized in that: The preparation steps include the following: (1) The mixed raw materials of processed Polygonum multiflorum, Ligustrum lucidum, Tribulus terrestris and ginseng are washed, dried, crushed and sterilized to obtain pretreated solid fermentation raw materials; (2) Aspergillus oryzae with accession number CICC 2386 was added to the pretreated solid-state fermentation raw material in step (1). Aspergillus oryzae The bacterial suspension was stirred and mixed thoroughly, and fermented at 32-35℃ for 48-96 hours with sterile air. Then, Monascus purpureus with preservation number CGMCC 41681 was added. Monascus pilosus Stir the bacterial suspension until well mixed, then ferment at 30-32℃ for 48-96 hours with sterile air, and then terminate the fermentation. (3) Extract the raw material after fermentation in step (2) with an ethanol aqueous solution, concentrate the extract to a thin paste, and redissolve the concentrate with pure water to obtain solution A; (4) Using the A solution obtained in step (3) as the extraction solvent, the mixed raw materials of Platycladus orientalis leaves, saffron, gromwell root, chuanxiong rhizome and sophora flavescens were heated and extracted. The resulting extract is the plant oil-controlling and hair loss-preventing composition. The mass ratio of processed Polygonum multiflorum, Ligustrum lucidum, Tribulus terrestris and ginseng in step (1) is as follows: processed Polygonum multiflorum 10-20 parts, Ligustrum lucidum 10-20 parts, Tribulus terrestris 2-10 parts, ginseng 2-10 parts; The volume content of ethanol in the aqueous ethanol solution in step (3) is 60-80%; the material-to-liquid ratio of the aqueous ethanol solution is 1:5-15, the extraction temperature is 60-80℃, and the extraction time is 1-3 h. The mass ratio of the following ingredients in step (4) is as follows: 2-10 parts of arborvitae leaf, saffron, gromwell root, chuanxiong rhizome and sophora flavescens root.

2. The preparation method of the plant-based oil-controlling and hair loss-preventing composition according to claim 1, characterized in that: The Aspergillus oryzae suspension mentioned in step (2) is prepared by the following method: Aspergillus oryzae was inoculated into PDA medium to activate the strain, and then transferred to modified Czapek's liquid medium for culture until the bacterial concentration reached 1×10⁻⁶. 8 A suspension of Aspergillus oryzae was obtained by adding cfu / mL or higher; the liquid-to-solid ratio of the Aspergillus oryzae suspension was 5-15%.

3. The preparation method of the plant-based oil-controlling and hair loss-preventing composition according to claim 1, characterized in that: The Monascus purpureus suspension mentioned in step (2) is prepared by the following method: All *Monascus purpureus* strains were inoculated onto PDA medium to activate the bacterial culture, and then transferred to esterase liquid medium for further culture until the bacterial concentration reached 1×10⁻⁶. 8 A suspension of Monascus purpureus was obtained by adding a concentration of CFU / mL or higher; the liquid-to-solid ratio of the Monascus purpureus suspension was 2-10%.

4. The preparation method of the plant-based oil-controlling and hair loss-preventing composition according to claim 1, characterized in that: The amount of pure water used for redissolving in step (3) is 5-15 times the amount of the concentrated substance.

5. The preparation method of the plant-based oil-controlling and hair loss-preventing composition according to claim 1, characterized in that: The heating extraction temperature in step (4) is 80-90℃ and the time is 1-3h; after the heating extraction, the supernatant is further filtered and centrifuged; then the concentration of the raw medicinal material in the extract is adjusted to 0.1-0.2g / g by adding pure water or heating and concentrating.

6. A plant-based oil-controlling and hair loss-preventing composition, characterized in that: It is prepared by the method described in any one of claims 1-5.

7. The application of the plant-based oil-controlling and hair loss-preventing composition according to claim 6 in the preparation of shampoo and conditioner products.

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