A sample pretreatment method for molecular fingerprinting of iron-bound organic carbon in sediments

CN121595294BActive Publication Date: 2026-06-09HKUST SHENZHEN RES INST

Patent Information

Authority / Receiving Office: CN · China
Patent Type: Patents(China)
Current Assignee / Owner: HKUST SHENZHEN RES INST
Filing Date: 2026-01-29
Publication Date: 2026-06-09

AI Technical Summary

Technical Problem

Existing technologies struggle to accurately separate and characterize iron-bound organic carbon in sediments without altering the chemical environment, leading to data mismatch and normalization complexity between EEM spectroscopy and HRMS, which impacts carbon sequestration stability assessments and climate change predictions.

Method used

A method of water extraction followed by acid extraction and then near-neutral reduction extraction was adopted. The sediment was treated with water, hydrochloric acid, sodium bicarbonate and sodium dithionite to ensure that the sample was compatible with three-dimensional fluorescence spectroscopy and high-resolution mass spectrometry detection. A PPL solid phase extraction column was used for desalting and elution to obtain comparable and reproducible molecular fingerprints.

Benefits of technology

It enables accurate separation and characterization of iron-bound organic carbon in sediments, ensures data verification between EEM and HRMS, improves signal-to-noise ratio and peak stability, and enhances the accuracy of carbon sequestration stability assessment and climate change prediction.

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Abstract

The present application relates to the technical field of environmental geochemistry research, and particularly relates to a sample pretreatment method for constructing molecular fingerprints of iron-bound organic carbon in sediments. The present application provides a sample pretreatment method for constructing molecular fingerprints of iron-bound organic carbon in sediments, wherein the sediments are sequentially subjected to water extraction, acid extraction and near-neutral reduction extraction in the order of weak to strong binding force and from non-destructive to selective destruction to extract iron-bound organic carbon. Not only can the method distinguish Fe-OC sub-fractions controlled by different forces and mineral phases, but also the obtained samples can be desalted by unified solid-phase extraction, and can be compatible with fluorescence three-dimensional spectrum (EEM) and high-resolution mass spectrometry (HRMS) molecular fingerprint characterization, so as to realize data mutual verification and molecular fingerprint acquisition of EEM and HRMS on the same sample.

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