A multi-zone culture device for mouse cells

Abstract

The application discloses a multi-zone culture device for mouse cells and relates to the technical field of cell culture devices, and aims to solve the technical problem of low functionality of the multi-zone culture device for mouse cells, comprising a body, a base, a heating column, a rotating mechanism, a pulse mechanism, a plurality of culture components and an auxiliary mechanism. The structure of the pulse mechanism is designed, so that the bottom disc can rotate relative to the top disc, so that the sliding block can slide back and forth on the centripetal groove, so that the mesh plate unit moves synchronously, the pressure of the culture cavity becomes larger and smaller, the pulse type dynamic pressure in the mouse body is simulated, and the accuracy and reliability of the related research on the mouse cell culture are improved. The technical problem of low functionality of the multi-zone culture device for mouse cells is solved.

Description

Technical Field

[0001] This invention relates to the field of cell culture device technology, and more specifically, to a multi-zone culture device for mouse cells. Background Technology

[0002] In the field of biomedical research, mouse cell culture is a core foundational method for revealing the laws of life activities, exploring disease mechanisms, and developing treatment options. Traditional cell culture methods are mostly static; however, with the deepening of research, scientists have gradually realized that the environment in which cells exist in vivo is dynamic and complex, and these dynamic environments have a crucial impact on cell growth, development, functional maintenance, and differentiation.

[0003] Cells in the body are not in a static environment, but are constantly stimulated by pulsed pressure from the heartbeat and vasoconstriction. For example, myocardial cells maintain rhythmic beating in the pressure fluctuations of the heart's contraction and relaxation. These dynamic pressure signals directly regulate cell morphogenesis, functional expression and metabolic balance.

[0004] However, current multi-zone culture devices on the market have limited functionality and most cannot accurately simulate the dynamic environment in vivo. When culturing cells sensitive to dynamic stress, such as cardiomyocytes, the lack of pulsed simulation leads to deviations in gene expression, cell function, and intercellular interactions from real-world conditions, severely impacting the accuracy and reliability of related research and hindering widespread application in general laboratories. Therefore, we propose a multi-zone culture device for mouse cells. Summary of the Invention

[0005] The purpose of this invention is to provide a multi-zone culture device for mouse cells to solve the technical problem of low functionality in multi-zone culture devices for mouse cells.

[0006] To solve the above-mentioned technical problems, the present invention provides the following technical solution: a multi-zone culture device for mouse cells, comprising a body and an auxiliary mechanism, wherein a base is fixedly mounted inside the body, a heating column is fixedly mounted at the top of the base, and a plurality of culture chambers are formed in a ring-shaped, equally spaced manner on the heating column, each culture chamber is provided with a culture component, and the plurality of culture chambers are connected by a circular cavity, the circular cavity being provided with a pulse mechanism; the pulse mechanism includes a connecting shaft, a top disk, and a bottom disk, the top disk being disposed at the top of the circular cavity and fixedly connected to the top of the connecting shaft, the top... The bottom of the disc has several guide grooves arranged in a ring at equal intervals. The top of the bottom disc has several centripetal grooves positioned opposite the guide grooves. Pressure units are slidably mounted on the centripetal grooves, with their tops movably connected to the guide grooves. The base has a rotating mechanism for driving the connecting shaft and the bottom disc to rotate relative to each other. The auxiliary mechanism includes several arc plates B, each fixedly mounted within a number of culture chambers. Each arc plate B has a through groove, and several baffle units are evenly arranged on the through groove, with the baffle units arranged in an inclined structure. This invention, through the above configuration, allows the bottom disc to rotate relative to the top disc, enabling the slider to slide back and forth on the centripetal grooves. This causes the mesh plate units to move synchronously, resulting in changes in pressure within the culture chambers, mimicking the pulsed dynamic pressure in a mouse. This improves the accuracy and reliability of research related to mouse cell culture and solves the technical problem of low functionality in multi-zone mouse cell culture devices. Furthermore, when several mesh plate units rotate and undergo pulsed respiration, the nutrient solution enters and exits through the inclined gaps between several baffle units, ensuring the stability of the pulsed dynamic pressure environment within the culture chamber, preventing the nutrient solution from directly impacting the mouse cells on the culture block, reducing the mortality rate of mouse cells, and thus ensuring the accuracy of related research on mouse cell culture.

[0007] Preferably, the base has a rotating groove A at its top, a rotating groove B at its bottom, a ring tooth groove at its bottom, and an installation groove at its bottom.

[0008] Preferably, the culture chamber has grooves at both ends on the eccentric side; The circular cavity is located off-center from the heating column, resulting in the unequal sizes of the various culture chambers.

[0009] Preferably, the rotating mechanism includes a motor, a central gear, a circular seat, several side gears, and a collar; the motor is fixedly mounted on the mounting groove, the central gear is rotatably mounted in the ring tooth groove via a rotating shaft A, the bottom end of the rotating shaft A passes through the mounting groove and is fixedly connected to the output shaft of the motor, the circular seat is rotatably mounted on the rotating groove B, the connecting shaft is rotatably mounted on the circular seat and is fixedly connected to the central gear, several side gears are rotatably mounted on the bottom end of the circular seat via rotating shaft B, the two ends of the side gears are respectively meshed with the central gear and the ring tooth groove, the collar is fixedly mounted on the top end of the circular seat, and the bottom disc is fixedly mounted on the top end of the collar and rotatably connected to the connecting shaft.

[0010] Preferably, a stirring unit is fixedly provided at a position relative to the gap between the plurality of pressure units on the connecting shaft.

[0011] Preferably, the guide groove has a V-shaped structure, and several guide grooves are connected to form a closed reciprocating channel; The pressure unit includes a slider slidably mounted on the centripetal groove. A vertical rod is fixedly mounted on the slider, and the top end of the vertical rod is movably connected to the sealed reciprocating channel via a movable ring. A connecting plate is fixedly mounted on the vertical rod, and a mesh plate unit is provided in the gap between two adjacent connecting plates. The mesh plate unit includes mesh plate A and mesh plate B, which are rotatably connected to the two connecting plates respectively. Mesh plate A and mesh plate B are rotatably connected, and both mesh plate A and mesh plate B are arc-shaped structures.

[0012] Preferably, the culture component includes an arc plate A, and a plurality of arc plates A are respectively disposed in a plurality of culture chambers. Both ends of the arc plate A are fixedly provided with slide bars, and the slide bars are slidably connected to the slide grooves. A groove is provided at the top of the arc plate A on the center side, and an embedding groove is provided at the bottom of the arc plate A on the center side, and a culture block is embedded in the embedding groove.

[0013] Preferably, the culture block is made of an elastic material, and the centripetal end of the culture block has a wave structure, with the interior of the culture block being hollow to form a deformation cavity.

[0014] Preferably, the baffle unit includes a plate body, which is rotatably connected to the through groove via a rotating rod. The bottom end of the rotating rod extends through the arc plate B and is fixed with a circular block. The bottom end of the circular block has an arc groove A and an arc groove B with a centrally symmetrical structure. The arc groove A and the arc groove B are connected to form a reciprocating rotation channel.

[0015] Preferably, the auxiliary mechanism further includes a linkage component, which includes a rotating ring rotatably mounted on the rotating groove A. The inner edge of the rotating ring is fixedly connected to the top of the circular seat by a number of telescopic rods. The top of the rotating ring is fixedly provided with a number of guide posts in an annular, equally spaced structure. The guide posts are movably engaged with the reciprocating rotation channel, and the gap between two adjacent guide posts is adapted to the diameter of the circular block.

[0016] The beneficial effects of this invention are: 1. This invention, through the structural design of the pulse mechanism, enables the bottom disk to rotate relative to the top disk, thereby allowing the slider to slide back and forth on the centripetal groove. This causes the mesh plate unit to move synchronously, resulting in the pressure in the culture chamber increasing and decreasing, mimicking the pulse-like dynamic pressure in a mouse. This improves the accuracy and reliability of research related to mouse cell culture and solves the technical problem of low functionality in multi-zone culture devices for mouse cells.

[0017] 2. This invention further designs the rotating mechanism and the pulse mechanism so that when the motor output shaft rotates, the central gear drives the connecting shaft and the top disk to rotate, and the collar drives the bottom disk to rotate differentially relative to the top disk. This causes the sealed reciprocating channel to rotate relative to several centripetal grooves, thereby causing the slider to slide back and forth on the centripetal grooves, the movable ring to move on the sealed reciprocating channel, and the connecting plate to move back and forth along the centripetal direction. This causes the mesh plate units to move synchronously. When the slider is at the eccentric end of the centripetal groove, several mesh plate units form a circular state. When the slider is not at the eccentric end of the centripetal groove, several mesh plates A and mesh plates B rotate relative to each other. This forms a V-shape, causing several mesh plate units to form a flower-like structure. It can be seen that when the bottom disk rotates relative to the top disk, the several mesh plate units form a rotatable pulse-like breathing structure. When the slider moves towards the eccentric end of the centripetal groove, it reduces the space between the outer side of the several mesh plate units and the circular cavity, pressing the culture medium into the culture chamber and increasing the pressure in the culture chamber. Conversely, when the slider moves towards the centripetal end of the centripetal groove, the pressure in the culture chamber decreases, mimicking the pulse-like dynamic pressure in a mouse. This improves the accuracy and reliability of research related to mouse cell culture and solves the technical problem of low functionality in multi-zone culture devices for mouse cells.

[0018] 3. This invention designs several culture chambers of unequal size, resulting in varying pressure ranges within each chamber. This achieves differentiated pulsed dynamic pressure within the chambers, facilitating comparative studies of mouse cell culture under different in vivo dynamic pressure environments. A single culture session can provide multiple sets of experimental data, thereby improving the efficiency of research related to mouse cell culture. Furthermore, the rotating pulsed respiration of the mesh plate units promotes homogenization of the culture medium and enhances its replacement within the chambers, improving the uniformity of the culture medium and ultimately benefiting mouse cell culture.

[0019] 4. By designing the culture block structure, this invention increases the surface area of ​​the culture block by setting the centripetal end to a wavy structure. This not only provides more attachment space for the bacteria, but also allows the deformation chamber to easily deform when the dynamic pressure environment changes within the culture chamber. Combined with the pulsed dynamic pressure, this creates vibrations, thereby simulating the growth and meristem dynamics of mouse cells. This further simulates the dynamic environment of mouse cell culture, further improving the accuracy and reliability of related research on mouse cell culture, and thus further enhancing the functionality of the multi-zone culture device for mouse cells.

[0020] 5. By setting up an auxiliary mechanism, the present invention enables the nutrient solution to enter and exit through the inclined gaps of several baffle units during the pulsating respiration of several mesh plate units, thereby ensuring the stability of the pulsating dynamic pressure environment in the culture chamber, preventing the nutrient solution from directly impacting the mouse cells on the culture block, reducing the mortality rate of mouse cells, and thus ensuring the accuracy of related research on mouse cell culture.

[0021] 6. Through the structural design of the baffle unit and linkage components, this invention enables the circular seat to drive the rotating ring and several guide posts to rotate via several telescopic rods, such as... Figure 11 As shown, only one guide post moves within the reciprocating rotary channel. When one guide post moves out of the reciprocating rotary channel, another guide post happens to enter and move within it, preventing the baffle unit from rotating on its own. Furthermore, when the guide post moves within arc groove A, it drives the circular block, rotating rod, and plate to rotate in the forward direction. When the guide post moves within arc groove B, it drives the circular block, rotating rod, and plate to rotate in the reverse direction, causing the baffle unit to perform a reciprocating rotary motion. This is equivalent to adding a "dynamic buffer curtain" to the nutrient solution. When the liquid passes through the channel, it is "dispersed" and "guided" by the constantly oscillating plate, further reducing the direct impact force of the nutrient solution. When the culture medium rotates forward, it exerts a force that "rolls" the fresh nutrient solution from the outside into the depths of the culture chamber; when it rotates backward, it exerts a force that "pushes" the metabolic waste from the inside towards the mesh plate unit for discharge. This dynamic mixing with the pulsed respiration action of several mesh plate units during rotation not only helps ensure the uniformity of nutrients in the nutrient solution near the culture block, but also facilitates the replacement of the nutrient solution near the culture block. The disturbance of the nutrient solution near the culture block further mimics the in vivo growth environment of mouse cells, compensating for the defects of static plates, making the dynamic environment of each culture chamber closer to the in vivo state of mice, and further reducing the deviation of experimental data in multi-zone culture. Attached Figure Description

[0022] Figure 1 This is a schematic diagram of the overall structure of the present invention.

[0023] Figure 2 This is a partial structural schematic diagram of the present invention.

[0024] Figure 3 This is a schematic diagram showing the disassembled structure of the base and heating column of the present invention.

[0025] Figure 4 This is a schematic diagram of the pulse mechanism of the present invention.

[0026] Figure 5 This is a partial structural schematic diagram of the pressure unit of the present invention.

[0027] Figure 6 This is a partial structural schematic diagram of the pulse mechanism of the present invention.

[0028] Figure 7 This is a partial structural cross-sectional schematic diagram of the base, rotating mechanism, pulse mechanism and auxiliary mechanism of the present invention.

[0029] Figure 8 This is a schematic diagram showing the disassembled structure of the culture component of the present invention.

[0030] Figure 9 This is a schematic diagram of the auxiliary mechanism of the present invention.

[0031] Figure 10 This is a partial structural breakdown diagram of the auxiliary mechanism of the present invention.

[0032] Figure 11 This is a schematic diagram of the motion state of the auxiliary mechanism of the present invention.

[0033] Figure 12 This is a schematic diagram of the heating column, pulse mechanism, culture component, and auxiliary mechanism of the present invention.

[0034] Explanation of the labels in the diagram: 1. Body; 2. Base; 3. Heating column; 4. Rotation mechanism; 5. Pulsing mechanism; 6. Culture assembly; 7. Auxiliary mechanism; 21. Rotary groove A; 22. Rotary groove B; 23. Ring tooth groove; 24. Mounting groove; 31. Culture chamber; 32. Groove; 33. Circular cavity; 41. Motor; 42. Central gear; 43. Round seat; 44. Side gear; 45. Collar; 51. Coupling; 52. Top disc; 53. Bottom disc; 54. Pressure unit; 511. Stirring unit; 521. Guide groove; 531. Centripetal groove; 540. Connecting plate; 541. Slider; 542. Upright; 543. Movable ring; 544. Mesh plate unit; 60. Groove; 61. Arc plate A; 62. Slider; 63. Embedded groove; 64. Culture block; 71. Arc plate B; 72. Through slot; 73. Baffle unit; 74. Linkage assembly; 731. Plate; 732. Rotating rod; 733. Circular block; 734. Arc groove A; 735. Arc groove B; 741. Rotary ring; 742. Telescopic rod; 743. Guide post. Detailed Implementation

[0035] like Figures 1 to 12 As shown, the present invention relates to a multi-zone culture device for mouse cells, comprising a body 1, a base 2, a heating column 3, a rotating mechanism 4, a pulse mechanism 5, several culture components 6, and an auxiliary mechanism 7.

[0036] In an embodiment of the present invention, reference is made to Figure 1 , Figure 2 and Figure 3 As shown, the base 2 is fixed inside the body 1. The top of the base 2 is provided with a rotating groove A21, the bottom of the rotating groove A21 is provided with a rotating groove B22, the bottom of the rotating groove B22 is provided with a ring tooth groove 23, and the bottom of the base 2 is provided with a mounting groove 24.

[0037] In an embodiment of the present invention, reference is made to Figure 1 , Figure 2 and Figure 3 As shown, the heating column 3 is fixed at the top of the base 2. The heating column 3 has several culture chambers 31 with a ring-shaped and equally spaced structure. Both ends of the culture chamber 31 on the eccentric side are provided with sliding grooves 32. The culture chambers 31 are connected by circular cavities 33. The circular cavities 33 are located at the eccentric position of the heating column 3, so that the sizes of the culture chambers 31 are not equal.

[0038] In an embodiment of the present invention, reference is made to Figure 7 As shown, the rotating mechanism 4 includes a motor 41, a central gear 42, a circular seat 43, several side gears 44, and a collar 45. The motor 41 is fixedly mounted on the mounting groove 24. The central gear 42 is rotatably mounted in the ring tooth groove 23 via a rotating shaft A. The bottom end of the rotating shaft A passes through the mounting groove 24 and is fixedly connected to the output shaft of the motor 41. The circular seat 43 is rotatably mounted on the rotating groove B22. Several side gears 44 are rotatably mounted on the bottom end of the circular seat 43 via rotating shaft B. The two ends of the side gears 44 are respectively meshed with the central gear 42 and the ring tooth groove 23. The collar 45 is fixedly mounted on the top end of the circular seat 43. Through the structural design of the rotating mechanism 4, the present invention enables the output shaft of the motor 41 to rotate through an external control structure. The rotating shaft A drives the central gear 42 to rotate, causing the several side gears 44 to rotate relative to the ring tooth groove 23, resulting in the rotation of the circular seat 43 and the collar 45. The collar 45 rotates at a differential speed with the central gear 42.

[0039] In an embodiment of the present invention, reference is made to Figures 1 to 7As shown, the pulse mechanism 5 includes a connecting shaft 51, a top disc 52, and a bottom disc 53. The connecting shaft 51 is rotatably mounted on the circular seat 43 and fixedly connected to the central gear 42. The top disc 52 is located at the top of the circular cavity 33 and fixedly connected to the top of the connecting shaft 51. The bottom end of the top disc 52 has a ring-shaped structure with several guide grooves 521 at equal intervals. The bottom disc 53 is fixedly mounted on the top of the collar 45 and rotatably connected to the connecting shaft 51. The top of the bottom disc 53 has several centripetal grooves 531 at positions relative to the guide grooves 521. Pressure units 54 are slidably mounted on the centripetal grooves 531, and the top of the pressure units 54 is movably connected to the guide grooves 521.

[0040] In an embodiment of the present invention, a stirring unit 511 is fixedly mounted on the connecting shaft 51 at a position relative to the gap between several pressure units 54. In the present invention, according to the above principle, the connecting shaft 51 and the bottom disk 53 also rotate at a different speed, so that the stirring unit 511 and the pressure unit 54 rotate at a different speed, ensuring the uniformity of nutrients in the working liquid at the gap between several pressure units 54.

[0041] The guide groove 521 has a V-shaped structure, and several guide grooves 521 are connected to form a closed reciprocating channel.

[0042] The pressure unit 54 includes a slider 541, which is slidably mounted on the radial groove 531. A vertical rod 542 is fixedly mounted on the slider 541. The top end of the vertical rod 542 is movably connected to the sealed reciprocating channel through a movable ring 543. A connecting plate 540 is fixedly mounted on the vertical rod 542. A mesh plate unit 544 is provided in the gap between two adjacent connecting plates 540. The mesh plate unit 544 includes a mesh plate A and a mesh plate B. The mesh plate A and the mesh plate B are rotatably connected to the two connecting plates 540 respectively. The mesh plate A and the mesh plate B are rotatably connected, and both the mesh plate A and the mesh plate B are arc-shaped structures. This invention, through the structural design of the pulse mechanism 5, enables the central gear 42 to drive the connecting shaft 51 and the top disk 52 to rotate, and the collar 45 to drive the bottom disk 53 to rotate differentially relative to the top disk 52. This causes the sealed reciprocating channel to rotate relative to several centripetal grooves 531, thereby causing the slider 541 to slide back and forth on the centripetal grooves 531. The movable ring 543 moves on the sealed reciprocating channel, and the connecting plate 540 moves back and forth along the centripetal direction, thereby causing the mesh plate units 544 to move synchronously. When the slider 541 is located at the eccentric end of the centripetal groove 531, the several mesh plate units 544 form a circular state. When the slider 541 is not located at the eccentric end of the centripetal groove 531, several mesh plates A and mesh plates B rotate relative to each other and form a V-shape. The shape allows several mesh plate units 544 to form a flower-like pattern. It can be seen that when the bottom disk 53 rotates relative to the top disk 52, the several mesh plate units 544 form a rotatable pulse-like breathing structure. When the slider 541 moves towards the eccentric end of the centripetal groove 531, the gap between the outer side of the several mesh plate units 544 and the circular cavity 33 is reduced, and the culture pressure is pushed into the culture chamber 31, making the pressure in the culture chamber 31 increase. Conversely, when the slider 541 moves towards the centripetal end of the centripetal groove 531, the pressure in the culture chamber 31 decreases, mimicking the pulse-like dynamic pressure in a mouse, thereby improving the accuracy and reliability of related research on mouse cell culture and solving the technical problem of low functionality of multi-zone culture devices for mouse cells. It is worth mentioning that the different sizes of the various culture chambers 31 result in varying pressure ranges, achieving differentiated pulsed dynamic pressure within each chamber. This facilitates comparative studies of mouse cell culture under different in vivo dynamic pressure environments, allowing for multiple sets of experimental data from a single culture run, thereby improving the efficiency of mouse cell culture research. Furthermore, the rotating pulsed respiration of the mesh plate units 544 promotes homogenization of the culture medium and enhances the replacement effect of the culture medium within the culture chambers 31, improving the uniformity of the culture medium and thus benefiting mouse cell culture.

[0043] In an embodiment of the present invention, reference is made to Figure 1 , Figure 2 and Figure 8As shown, the culture assembly 6 includes an arc plate A61, with several arc plates A61 respectively disposed within several culture chambers 31. Sliding strips 62 are fixed at both ends of the arc plate A61, and the sliding strips 62 are slidably connected to the sliding grooves 32. A notch 60 is formed at the top of the arc plate A61 on its inward-facing side, and an embedding groove 63 is formed at the bottom of the arc plate A61 on its inward-facing side, with a culture block 64 embedded in the embedding groove 63. The notch 60 facilitates the removal of the culture assembly 6 in this invention.

[0044] The culture block 64 is made of an elastic material, and its centripetal end has a wavy structure, with a hollow interior forming a deformation cavity. This invention, by setting the centripetal end of the culture block 64 to have a wavy structure, increases the surface area of ​​the culture block 64, providing more attachment space for the microbial community. Furthermore, in conjunction with the deformation cavity, it easily deforms under dynamic pressure changes within the culture cavity 31, creating vibrations with pulsed dynamic pressure. This simulates the dynamic growth of the medullary layer in mouse cells, further mimicking the dynamic environment of mouse cell culture, thereby improving the accuracy and reliability of related research on mouse cell culture, and ultimately enhancing the functionality of the multi-zone mouse cell culture device.

[0045] In an embodiment of the present invention, reference is made to Figure 1 , Figure 2 , Figure 7 , Figure 10 and Figure 11 As shown, the auxiliary mechanism 7 includes several arc plates B71, which are fixedly installed in several culture chambers 31. Each arc plate B71 has a through groove 72, and several baffle units 73 are evenly arranged on the through groove 72 in an inclined configuration. By setting up the auxiliary mechanism 7, this invention ensures that when the several mesh plate units 544 rotate and undergo pulsed respiration, the nutrient solution enters and exits through the inclined gaps between the baffle units 73. This maintains the stability of the pulsed dynamic pressure environment within the culture chambers 31, prevents the nutrient solution from directly impacting the mouse cells on the culture block 64, reduces the mortality rate of the mouse cells, and thus ensures the accuracy of related research on mouse cell culture.

[0046] The baffle unit 73 includes a plate 731. The plate 731 and the through groove 72 are rotatably connected by a rotating rod 732. The bottom end of the rotating rod 732 passes through the arc plate B71 and is fixed with a round block 733. The bottom end of the round block 733 has an arc groove A734 and an arc groove B735 with a central symmetrical structure. The arc groove A734 and the arc groove B735 are connected to form a reciprocating rotation channel.

[0047] The auxiliary mechanism 7 also includes a linkage component 74, which includes a rotating ring 741 rotatably mounted on the rotating groove A21. The inner edge of the rotating ring 741 is fixedly connected to the top of the circular seat 43 via several telescopic rods 742. The top of the rotating ring 741 has several guide posts 743 fixedly mounted in an annular, equally spaced structure. The guide posts 743 are movably engaged with the reciprocating rotation channel, and the distance between two adjacent guide posts 743 is adapted to the diameter of the circular block 733. Through the structural design of the baffle unit 73 and the linkage component 74, this invention enables the circular seat 43 to drive the rotating ring 741 and the guide posts 743 to rotate via several telescopic rods 742. Figure 11 As shown, only one guide post 743 moves within the reciprocating rotary channel. When guide post 743 moves out of one reciprocating rotary channel, another guide post 743 happens to enter the same reciprocating rotary channel, preventing the baffle unit 73 from rotating on its own. Furthermore, when guide post 743 moves within arc groove A734, it drives the circular block 733, rotating rod 732, and plate 731 to rotate in the forward direction. When guide post 743 moves within arc groove B735, it drives the circular block 733, rotating rod 732, and plate 731 to rotate in the reverse direction, causing the baffle unit 73 to perform a reciprocating rotary motion. This is equivalent to adding a "dynamic buffer curtain" to the nutrient solution. When the liquid passes through the channel 72, it is "dispersed" and "guided" by the constantly oscillating plate 731, further reducing... The direct impact of the nutrient solution, and the force that "rolls" the fresh nutrient solution from the outside into the depth of the culture chamber 31 when the plate 731 rotates in the forward direction; and the force that "pushes" the metabolic waste from the inside towards the mesh plate unit 544 when it rotates in the reverse direction, dynamically mixes with the pulse-like breathing action of several mesh plate units 544 rotating. This not only helps to ensure the uniformity of nutrient solution near the culture block 64, but also helps to replace the nutrient solution near the culture block 64. The disturbance of the nutrient solution near the culture block 64 further mimics the in vivo growth environment of mouse cells, making up for the defects of the static plate 731, making the dynamic environment of each culture chamber 31 closer to the state in the mouse's body, and further reducing the experimental data deviation of multi-zone culture.

[0048] Working principle: This embodiment provides a multi-zone culture device for mouse cells. When in use, mouse cells are placed on the surface of culture block 64, and several culture chambers 31 and circular chambers 33 inside the body 1 are filled with nutrient solution. The cover of the body 1 is then closed. The output shaft of motor 41 is rotated by an external control structure, causing the collar 45 and the central gear 42 to rotate at different speeds. The central gear 42 drives the connecting shaft 51 and the top disk 52 to rotate. The collar 45 drives the bottom disk 53 to rotate at different speeds relative to the top disk 52, causing the closed reciprocating channel to rotate relative to several centripetal grooves 531. This causes the slider 541 to slide back and forth on the centripetal grooves 531, the movable ring 543 to move on the closed reciprocating channel, and the connecting plate 540 to move back and forth along the centripetal direction. This causes several mesh plate units 544 to form a rotatable pulsed breathing action, mimicking the pulsed dynamic pressure in a mouse. The sizes of several culture chambers 31 are not equal, so the size of their pressure ranges varies. This achieves differentiated pulsed dynamic pressure in several culture chambers 31, which is convenient for studying the culture comparison of mouse cells under different in vivo dynamic pressure environments. A single culture can provide multiple sets of experimental data. The circular base 43 drives the rotating ring 741 and the guide post 743 to rotate through several telescopic rods 742, so that the baffle unit 73 can perform a reciprocating rotation.

[0049] The embodiments disclosed in this invention are preferred embodiments, but are not limited thereto. Those skilled in the art can easily understand the spirit of this invention based on the above embodiments and make different extensions and variations, but as long as they do not depart from the spirit of this invention, they are all within the protection scope of this invention.

Claims

1. A multi-zone culture device for mouse cells, characterized in that, The device includes a body (1) and an auxiliary mechanism (7). A base (2) is fixed inside the body (1). A heating column (3) is fixed at the top of the base (2). Several culture chambers (31) are opened in a ring-shaped and equally spaced structure on the heating column (3). A culture component (6) is provided on the culture chamber (31). Several culture chambers (31) are connected through a circular cavity (33). A pulse mechanism (5) is provided on the circular cavity (33). The pulse mechanism (5) includes a connecting shaft (51), a top disc (52) and a bottom disc (53). The top disc (52) is located at the top of the cavity (33) and is fixedly connected to the top of the connecting shaft (51). The bottom of the top disc (52) has a ring-shaped structure with a plurality of guide grooves (521) at equal intervals. The top of the bottom disc (53) has a plurality of centripetal grooves (531) at positions relative to the plurality of guide grooves (521). A pressure unit (54) is slidably provided on the centripetal groove (531). The top of the pressure unit (54) is movably connected to the guide groove (521). The guide groove (521) has a V-shaped structure, and several guide grooves (521) are connected to form a closed reciprocating channel. The pressure unit (54) includes a slider (541), which is slidably disposed on the centripetal groove (531). A vertical rod (542) is fixedly disposed on the slider (541). The top end of the vertical rod (542) is movably connected to the sealed reciprocating channel through a movable ring (543). A connecting plate (540) is fixedly disposed on the vertical rod (542). A mesh plate unit (544) is provided in the gap between two adjacent connecting plates (540). The mesh plate unit (544) includes a mesh plate A and a mesh plate B. The mesh plate A and the mesh plate B are rotatably connected to the two connecting plates (540) respectively. The mesh plate A and the mesh plate B are rotatably connected, and both the mesh plate A and the mesh plate B are arc-shaped structures. The base (2) is provided with a rotating mechanism (4) for driving the connecting shaft (51) and the bottom disk (53) to rotate relative to each other. The auxiliary mechanism (7) includes several arc plates B (71), which are respectively fixed in several culture chambers (31). The arc plates B (71) are provided with through grooves (72), and several baffle units (73) are evenly provided on the through grooves (72). The baffle units (73) are arranged in an inclined structure.

2. The multi-zone culture device for mouse cells according to claim 1, characterized in that, The base (2) has a rotating groove A (21) at the top, a rotating groove B (22) at the bottom, a ring tooth groove (23) at the bottom, and an installation groove (24) at the bottom.

3. The multi-zone culture device for mouse cells according to claim 1, characterized in that, The culture chamber (31) has grooves (32) at both ends on the eccentric side. The circular cavity (33) is located off-center from the heating column (3), resulting in the unequal size of the space of the several culture cavities (31).

4. The multi-zone culture device for mouse cells according to claim 2, characterized in that, The rotating mechanism (4) includes a motor (41), a central gear (42), a circular seat (43), several side gears (44), and a collar (45); the motor (41) is fixedly mounted on the mounting groove (24), the central gear (42) is rotatably mounted in the ring gear groove (23) via a rotating shaft A, the bottom end of the rotating shaft A passes through the mounting groove (24) and is fixedly connected to the output shaft of the motor (41), and the circular seat (43) is rotatably mounted on the rotating groove B (22). The connecting shaft (51) is rotatably mounted on the round seat (43) and fixedly connected to the central gear (42). Several side gears (44) are rotatably mounted on the bottom end of the round seat (43) via the rotating shaft B. The two ends of the side gears (44) are respectively meshed with the central gear (42) and the ring tooth groove (23). The collar (45) is fixedly mounted on the top end of the round seat (43). The bottom disc (53) is fixedly mounted on the top end of the collar (45) and rotatably connected to the connecting shaft (51).

5. The multi-zone culture device for mouse cells according to claim 1, characterized in that, The connecting shaft (51) is fixed with a stirring unit (511) at a gap position relative to the pressure units (54).

6. The multi-zone culture device for mouse cells according to claim 3, characterized in that, The culture component (6) includes an arc plate A (61), and several arc plates A (61) are respectively disposed in several culture chambers (31). Both ends of the arc plate A (61) are fixedly provided with slide bars (62), and the slide bars (62) are slidably connected to the slide grooves (32). A groove (60) is provided on the top of the arc plate A (61) on the inward side, and a groove (63) is provided on the bottom of the arc plate A (61) on the inward side. A culture block (64) is embedded in the groove (63).

7. The multi-zone culture device for mouse cells according to claim 6, characterized in that, The culture block (64) is made of elastic material, and the centripetal end of the culture block (64) has a wave structure, and the interior of the culture block (64) is hollow to form a deformation cavity.

8. The multi-zone culture device for mouse cells according to claim 4, characterized in that, The baffle unit (73) includes a plate (731), the plate (731) and the through groove (72) are rotatably connected by a rotating rod (732), the bottom end of the rotating rod (732) passes through the arc plate B (71) and is fixed with a round block (733), the bottom end of the round block (733) has an arc groove A (734) and an arc groove B (735) with a central symmetrical structure, the arc groove A (734) and the arc groove B (735) are connected to form a reciprocating rotation channel.

9. The multi-zone culture device for mouse cells according to claim 8, characterized in that, The auxiliary mechanism (7) further includes a linkage component (74), which includes a rotating ring (741). The rotating ring (741) is rotatably mounted on the rotating groove A (21). The inner edge of the rotating ring (741) is fixedly connected to the top of the circular seat (43) by several telescopic rods (742). The top of the rotating ring (741) is fixedly provided with several guide posts (743) in an annular, equally spaced structure. The guide posts (743) are movably engaged with the reciprocating rotating channel, and the gap between two adjacent guide posts (743) is adapted to the diameter of the circular block (733).

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