A cross-linked porous polymer for detecting tryptamine and a preparation method and application thereof

Abstract

The application provides a cross-linked porous polymer for detecting tryptamine and a preparation method and application thereof, the cross-linked porous polymer comprises a BODIPY fluorescent component and a triphenylamine electron-withdrawing component, has a pore structure, a large specific surface area and an extended pi conjugated system, and the BODIPY fluorescent component and the triphenylamine electron-withdrawing component are connected through an alkynyl group. In the preparation method, the pore size distribution of the material can be adjusted by a reaction solvent, not only the adsorption enrichment capacity of the target analyte is improved, but also the mass transfer and diffusion rate of the analyte in the material is accelerated. The unique extended pi conjugated skeleton structure can improve the fluorescence signal change amplitude before and after the target detection, and the three-dimensional covalent cross-linked network endows the material with excellent chemical and light stability. The fluorescent sensing material organically combines the functions of adsorption enrichment, signal amplification and good stability, and realizes the high-sensitivity, rapid and stable detection of tryptamine.

Description

Technical Field

[0001] This invention relates to the field of fluorescence sensing materials technology, and in particular to a cross-linked porous polymer for detecting tryptamine, its preparation method and application. Background Technology

[0002] Tryptophan is a novel psychoactive substance, a monoamine alkaloid with an indole ring structure. Ingestion of small amounts can cause symptoms such as dilated pupils, nausea, jaw clenching, excessive muscle tension, high blood pressure, and tachycardia by binding to serotonin receptors in the body. Therefore, rapid detection of tryptophan is of great significance.

[0003] Currently developed methods for tryptamine detection include chromatography-mass spectrometry, Raman spectroscopy, and electrochemical methods. These methods suffer from drawbacks such as large instrument requirements, complex sample pretreatment, the influence of interfering substances on detection results, and high costs. Fluorescence sensing technology, with its advantages of high sensitivity, simple operation, rapid response, and ease of visual identification, is one of the most promising new technologies for tryptamine detection.

[0004] This type of technology relies on the construction of fluorescent sensing materials. The interaction between the material and the target analyte induces changes in optical properties, enabling the detection of the target substance. However, the slow diffusion rate of the target analyte in the bulk material, its low effective concentration, and the small amplitude of fluorescence signal changes induced during the recognition process result in most fluorescent sensing materials still exhibiting drawbacks such as low detection sensitivity and slow response speed. Furthermore, there are currently no indications of using cross-linked porous polymers for tryptamine detection. Summary of the Invention

[0005] This invention provides a cross-linked porous polymer for detecting tryptamine, its preparation method, and its application, addressing the shortcomings of existing technologies. This polymer comprises a BODIPY-based fluorescent component and a triphenylamine electron-withdrawing component, possessing a porous structure, large specific surface area, and an extended π-conjugated system. The polymer is prepared by using a BODIPY-based dye as monomer one and tris(4-ethynylphenyl)amine as monomer two, through a polycondensation reaction between the two. The detection method uses a cross-linked porous polymer and an organic solvent as the detection reagent. Upon addition of tryptamine, the fluorescence intensity of the detection reagent changes significantly, exhibiting advantages such as high sensitivity, fast response time, simple operation, and good stability.

[0006] This invention provides a cross-linked porous polymer for detecting tryptamines. The cross-linked porous polymer contains a BODIPY-type fluorescent component and a triphenylamine electron-withdrawing component, and has a pore structure, a large specific surface area, and an extended π-conjugated system. The BODIPY-type fluorescent component and the triphenylamine electron-withdrawing component are linked by alkynyl groups.

[0007] According to the present invention, a cross-linked porous polymer for detecting tryptamines, wherein the BODIPY-type fluorescent component is characterized by having the following general structural formula:

[0008] Wherein, R1 is one of phenyl, methyl, methoxy, ethyl, propyl, or CF3; R2 is one of Br or I. A method for preparing a cross-linked porous polymer for detecting tryptamine, as described above, is provided. Monomer 1 and monomer 2 are dissolved in an organic solvent by stirring in a predetermined ratio. Then, an alkaline additive is added, and the mixed solution is subjected to nitrogen removal. Under nitrogen protection, a catalyst consisting of CuI and tetrakis(triphenylphosphine)palladium catalyst is added, and the mixture is heated to 80-130°C and stirred for subsequent polymerization reaction for 48-72 hours. After the reaction is completed, the mixture is cooled to room temperature, and the precipitated network polymer is filtered. The polymer is washed sequentially with chloroform, water, methanol, and acetone, and the polymer is subjected to Soxhlet extraction and vacuum drying to obtain the cross-linked porous polymer.

[0009] According to the present invention, a method for preparing a cross-linked porous polymer for detecting tryptamine is provided, wherein monomer one is a BODIPY-type fluorescent component, monomer two is tris(4-ethynylphenyl)amine, and monomer one and monomer two are polymerized by a Sonogashira coupling reaction.

[0010] According to the present invention, a method for preparing a cross-linked porous polymer for detecting tryptamine is provided, wherein the organic solvent is one of toluene and N,N-dimethylformamide.

[0011] According to the present invention, a method for preparing a cross-linked porous polymer for detecting tryptamine is provided, wherein the type of organic solvent can be used to control the polymer pore structure.

[0012] According to the present invention, a method for preparing a cross-linked porous polymer for detecting tryptamine is provided, wherein the alkaline additive is one or more of triethylamine, diethylamine, and trimethylamine.

[0013] According to the present invention, a method for preparing a cross-linked porous polymer for detecting tryptamine is provided, wherein the ratio of monomer one to monomer two is one of 1:1 and 1.5:1.

[0014] According to the present invention, a method for preparing a cross-linked porous polymer for detecting tryptamine is provided, wherein the ratio of organic solvent to alkaline additive is 4:1.

[0015] According to the present invention, a method for preparing a cross-linked porous polymer for detecting tryptamine is provided, wherein the catalyst is CuI and tetra(triphenylphosphine)palladium.

[0016] According to the present invention, a method for preparing a cross-linked porous polymer for detecting tryptamine is provided, using toluene as an organic solvent, with a polymerization reaction temperature of 80-90℃ and a reaction time of 48-72 h. Alternatively, N,N-dimethylformamide can be used as an organic solvent, with a polymerization reaction temperature of 120-130℃ and a reaction time of 48-72 h.

[0017] According to the present invention, a method for preparing a cross-linked porous polymer for detecting tryptamine is provided, wherein the Soxhlet extraction time is 72 h.

[0018] According to the present invention, a method for preparing a cross-linked porous polymer for detecting tryptamine is provided, wherein the prepared cross-linked porous polymer contains both micropores and mesopores.

[0019] An application of the cross-linked porous polymer for detecting tryptamine as described above is provided, wherein the cross-linked porous polymer is dispersed in an organic solvent to form a detection reagent for fluorescence sensing detection of α-methyltryptamine.

[0020] The detection method for fluorescence sensing detection of α-methyltryptamine is performed according to the following steps: a) The obtained cross-linked porous polymer was added to the organic solvent methanol and ultrasonically dispersed to obtain a homogeneous detection reagent with a concentration of 0.1 mg / mL. The fluorescence emission spectrum under 480 nm excitation was then measured using a fluorescence spectrometer. b. Prepare a 1 mg / mL α-methyltryptamine methanol solution; c. Add different volumes of α-methyltryptamine methanol solution to the detection reagent obtained in step a in sequence. After each addition of α-methyltryptamine methanol solution, mix for 30 seconds and then measure the fluorescence emission spectrum under 480 nm excitation using a fluorescence spectrometer. d. Compare the changes in fluorescence intensity of the maximum emission peak in the fluorescence emission spectrum with the addition of different concentrations of the analyte, and calculate key parameters such as the detection limit.

[0021] The organic solvent used may be, but is not limited to, methanol.

[0022] This invention provides a cross-linked porous polymer for detecting tryptamine, its preparation method, and its application. The resulting cross-linked porous polymer fluorescent sensing material possesses a porous structure and a large specific surface area. The pore size distribution of the material can be controlled by adjusting the reaction solvent, which not only enhances the adsorption and enrichment capacity of the target analyte but also accelerates the mass transfer and diffusion rate of the analyte within the material. Its unique extended π-conjugated framework structure can improve the amplitude of fluorescence signal changes before and after target analyte detection, and the three-dimensional covalent cross-linked network endows the material with excellent chemical / photometric stability. The fluorescent sensing material organically combines adsorption enrichment, signal amplification, and good stability, achieving highly sensitive, rapid, and stable detection of tryptamine. Attached Figure Description

[0023] To more clearly illustrate the technical solutions in this invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of this invention. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0024] Figure 1 The infrared spectra of the BODIPY fluorescent component, tris(4-ethynylphenyl)amine, and cross-linked porous polymer in Example 1 of this invention are shown below. Figure 2 The solid-state carbon NMR spectra of the BODIPY fluorescent component, tris(4-ethynylphenyl)amine, and cross-linked porous polymer in Example 1 of this invention are shown below. Figure 3 This is a SEM image of the cross-linked porous polymer in Example 1 of the present invention; Figure 4 The attached diagram shows the nitrogen adsorption / desorption of the cross-linked porous polymer in Example 1 of this invention; Figure 5 This is a pore size distribution diagram of the cross-linked porous polymer in Example 1 of the present invention; Figure 6 The image shows the fluorescence emission spectra of different concentrations of α-methyltryptamine detected by the cross-linked porous polymer in Example 1 of this invention. Figure 7 This is a graph showing the maximum emission peak intensity versus α-methyltryptamine concentration curves for detecting different concentrations of α-methyltryptamine in the cross-linked porous polymer of Example 1 of the present invention. Detailed Implementation

[0025] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. The components of the embodiments of the present invention described and shown in the accompanying drawings can generally be arranged and designed in various different configurations.

[0026] Therefore, the following detailed description of the embodiments of the invention provided in the accompanying drawings is not intended to limit the scope of the claimed invention, but merely to illustrate selected embodiments of the invention. All other embodiments obtained by those skilled in the art based on the embodiments of the invention without inventive effort are within the scope of protection of the invention.

[0027] It should be noted that similar reference numerals and letters in the following figures indicate similar items; therefore, once an item is defined in one figure, it does not need to be further defined and explained in subsequent figures. Furthermore, the terms "first," "second," etc., are used only to distinguish descriptions and should not be construed as indicating or implying relative importance.

[0028] In the description of the embodiments of the present invention, it should be noted that the terms "inner", "outer", "upper", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, or the orientation or positional relationship in which the product of the invention is usually placed when in use. They are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limiting the present invention.

[0029] Example 1

[0030] A method for preparing a cross-linked porous polymer for detecting tryptophan includes the following steps: 48.2 mg of (T-4)-[3-bromo-5-[(4-bromo-3,5-dimethyl-2H-pyrrolidinone-κN)benzyl]-2,4-dimethyl-1Hpyrrolidone-κN]difluoroboron and 31.7 mg of tris(4-ethynylphenyl)amine were added to 8 mL of toluene and stirred to dissolve. Then, 2 mL of triethylamine was added. The mixture was denitrogenated, and under nitrogen protection, 4 mg of CuI and 6 mg of tetrakis(triphenylphosphine)palladium were added sequentially. The mixture was heated to 80 °C and reacted for 72 h. After the reaction was complete, the mixture was cooled to room temperature, and the precipitated network polymer was filtered. The polymer was washed sequentially with chloroform, water, methanol, and acetone, and then subjected to Soxhlet extraction with tetrahydrofuran for 72 h. The polymer was dried to obtain a cross-linked porous polymer.

[0031] The infrared spectra of (T-4)-[3-bromo-5-[(4-bromo-3,5-dimethyl-2H-pyrrole-2-ylidene-κN)benzyl]-2,4-dimethyl-1Hpyrrolidone-κN]difluoroboron, tris(4-ethynylphenyl)amine, and cross-linked porous polymers were compared. A 1344 cm⁻¹ spectrum attributable to BF was observed in (T-4)-[3-bromo-5-[(4-bromo-3,5-dimethyl-2H-pyrrole-2-ylidene-κN)benzyl]-2,4-dimethyl-1Hpyrrolidone-κN]difluoroboron. -1 Peak. Tris(4-ethynylphenyl)amine at 2100 cm⁻¹ -1 Alkyne peaks appear at 1498 and 1601 cm⁻¹. -1 A C=C skeletal vibration peak of the benzene ring appears at 1310 cm⁻¹. -1 CN bond stretching vibrations appear at this point. In the infrared characteristic spectrum of cross-linked porous polymers, at 2100 cm⁻¹... -1The peak shift of the alkynyl group was 2186 cm⁻¹. -1 The presence of characteristic peaks of the monomers indicates that polymerization has successfully occurred between the two monomers.

[0032] The chemical structure of the cross-linked porous polymer was characterized using nuclear magnetic resonance spectroscopy, such as... Figure 2 As shown, in the solid-state carbon NMR spectrum, the two different regions at 70-90 ppm correspond to two alkynyl carbon atoms, the two peaks at 110-120 ppm correspond to carbon atoms bonded to the alkynyl group, and the peak at 12 ppm corresponds to the pentamethyl-substituted sp in the BODIPY core. 3 Methyl groups. Furthermore, the two distinct regions of 120-130 and 140 ppm belong to the two monomeric components. 2 Carbon atoms. These results further confirm the successful preparation of cross-linked porous polymers.

[0033] Scanning electron microscope (SEM) image ( Figure 3 The image visually displays the microstructure of the cross-linked porous polymer. As can be seen from the image, the polymer has a highly cross-linked three-dimensional network structure, in which the network structure is composed of nanowires.

[0034] The key parameters of the prepared cross-linked porous polymer, such as pore structure, specific surface area, and pore volume, were obtained by BET characterization. Figure 4 The attached graph shows the nitrogen adsorption / desorption of the polymer at 77.3 K, exhibiting Type I and Type IV characteristics. Specifically, it shows a significant nitrogen adsorption peak under low relative pressure conditions, while a distinct hysteresis loop appears in the high relative pressure range, indicating that the prepared polymer possesses both microporous and mesoporous structures. The specific surface area of ​​the polymer, calculated using the Brunauer-Emmet-Teller (BET) method, is as high as 698.3 m². 2 / g. From Figure 5 The pore size distribution curves show that the polymer contains a large number of microporous structures and a small number of mesoporous structures. The pore sizes of the microporous structures are 0.76 nm, 1.26 nm, and 1.48 nm, respectively, while the pore size of the mesoporous structures is 2.6 nm. The total pore volume is 0.713 cm³. 3 / g.

[0035] Example 2

[0036] A method for preparing a cross-linked porous polymer for detecting tryptophan includes the following steps: 48.2 mg of (T-4)-[3-bromo-5-[(4-bromo-3,5-dimethyl-2H-pyrrolidinone-κN)benzyl]-2,4-dimethyl-1Hpyrrolidone-κN]difluoroboron and 31.7 mg of tris(4-ethynylphenyl)amine were added to 8 mL of N,N-dimethylformamide and stirred until dissolved. Then, 2 mL of triethylamine was added. The mixture was denitrogenated, and under nitrogen protection, 4 mg of CuI and 6 mg of tetra(triphenylphosphine)palladium were added sequentially. The mixture was heated to 130 °C and reacted for 72 h. After the reaction was complete, the mixture was cooled to room temperature, and the precipitated network polymer was filtered. The polymer was washed sequentially with chloroform, water, methanol, and acetone, and then subjected to Soxhlet extraction with tetrahydrofuran for 72 h. The polymer was dried to obtain a cross-linked porous polymer with pore sizes of 1.63 nm and 3.02 nm.

[0037] Example 3

[0038] A method for preparing a cross-linked porous polymer for detecting tryptophan includes the following steps: 72.3 mg of (T-4)-[3-bromo-5-[(4-bromo-3,5-dimethyl-2H-pyrrolidinone-κN)benzyl]-2,4-dimethyl-1Hpyrrolidone-κN]difluoroboron and 31.7 mg of tris(4-ethynylphenyl)amine were added to 8 mL of toluene and stirred to dissolve. Then, 2 mL of triethylamine was added. The mixture was denitrogenated, and under nitrogen protection, 4 mg of CuI and 6 mg of tetra(triphenylphosphine)palladium were added sequentially. The mixture was heated to 130 °C and reacted for 72 h. After the reaction was complete, the mixture was cooled to room temperature, and the precipitated network polymer was filtered. The polymer was washed sequentially with chloroform, water, methanol, and acetone, and then subjected to Soxhlet extraction with tetrahydrofuran for 72 h. The polymer was dried to obtain a cross-linked porous polymer with pore sizes of 0.81 nm, 1.33 nm, and 2.4 nm.

[0039] Example 4

[0040] A method for preparing a cross-linked porous polymer for detecting tryptophan includes the following steps: 48.2 mg of (T-4)-[3-bromo-5-[(4-bromo-3,5-dimethyl-2H-pyrrolidinone-κN)benzyl]-2,4-dimethyl-1Hpyrrolidone-κN]difluoroboron and 31.7 mg of tris(4-ethynylphenyl)amine were added to 8 mL of toluene and stirred to dissolve. Then, 2 mL of triethylamine was added. The mixture was subjected to nitrogen removal, and under nitrogen protection, 4 mg of CuI and 6 mg of tetra(triphenylphosphine)palladium were added sequentially. The mixture was heated to 130 °C and reacted for 48 h. After the reaction was complete, the mixture was cooled to room temperature, and the precipitated network polymer was filtered. The polymer was washed sequentially with chloroform, water, methanol, and acetone, and then subjected to Soxhlet extraction with tetrahydrofuran for 72 h. The polymer was dried to obtain a cross-linked porous polymer with pore sizes of 1.15 nm and 2.32 nm.

[0041] Example 5

[0042] A method for preparing a cross-linked porous polymer for detecting tryptophan includes the following steps: 48.2 mg of (T-4)-[3-iodo-5-[(4-iodo-3,5-dimethyl-2H-pyrrolidinone-κN)benzyl]-2,4-dimethyl-1Hpyrrolidone-κN]difluoroboron and 31.7 mg of tris(4-ethynylphenyl)amine were added to 8 mL of toluene and stirred to dissolve. Then, 2 mL of triethylamine was added. The mixture was denitrogenated, and under nitrogen protection, 4 mg of CuI and 6 mg of tetra(triphenylphosphine)palladium were added sequentially. The mixture was heated to 130 °C and reacted for 48 h. After the reaction was complete, the mixture was cooled to room temperature, and the precipitated network polymer was filtered. The polymer was washed sequentially with chloroform, water, methanol, and acetone, and then subjected to Soxhlet extraction with tetrahydrofuran for 72 h. The polymer was dried to obtain a cross-linked porous polymer with pore sizes of 0.93 nm, 1.52 nm, and 2.89 nm.

[0043] Example 6

[0044] An application of cross-linked porous polymers for detecting tryptamines includes the following steps: The cross-linked porous polymer prepared in Example 1 was added to the organic solvent methanol and ultrasonically dispersed to obtain a homogeneous detection reagent with a concentration of 0.1 mg / mL. 3.5 mL of the detection reagent was added to a cuvette, and the fluorescence emission spectrum of the detection reagent at an excitation wavelength of 480 nm was measured; this was the initial fluorescence emission spectrum without α-methyltryptamine molecules.

[0045] A 1 mg / mL α-methyltryptamine methanol solution was prepared. 5 μL of the α-methyltryptamine solution was added to the detection reagent, and the fluorescence emission spectrum of the mixture was measured after 30 seconds. Then, 10 μL, 10 μL, and 10 μL of α-methyltryptamine solution were added sequentially, and the fluorescence emission spectrum of the mixture was measured after 30 seconds. Finally, 30 μL and 30 μL of α-methyltryptamine solution were added, and the fluorescence emission spectrum of the mixture was measured after 30 seconds. This yielded a schematic diagram illustrating the detection effect of the cross-linked porous polymer on different concentrations of α-methyltryptamine. Figure 6 As shown. Record the fluorescence intensity at 620 nm in the different fluorescence emission spectra obtained from the above tests, using it as the ordinate and the concentration of the mixed solution as the abscissa, and plot the curve, as shown. Figure 7 As shown, the fluorescence intensity of the cross-linked porous polymer exhibits a good linear relationship with the concentration of α-methyltryptamine, which may be due to the polymer's large specific surface area and numerous pore structures, resulting in strong adsorption performance for α-methyltryptamine. Using the formula LOD = 3S / D (where S is the standard deviation of the fluorescence intensity of the blank sample, and m is the slope of fluorescence intensity versus α-methyltryptamine concentration), the detection limit was calculated to be 1 μg / mL.

[0046] In summary, the cross-linked porous polymer for detecting tryptamine proposed in this invention, along with its preparation method and application, offers the following advantages: The polymer contains a BODIPY-based fluorescent component and a triphenylamine electron-withdrawing component, possessing a porous structure, large specific surface area, and an extended π-conjugated system. It can be prepared by a condensation reaction between a BODIPY-based dye as monomer one and tris(4-ethynylphenyl)amine as monomer two. The preparation process is simple, and the pore structure can be controlled by adjusting the reaction solvent ratio, increasing the diffusion rate and effective concentration of the target analyte in the bulk material. The cross-linked porous polymer, combined with an organic solvent, forms a detection reagent. Upon addition of tryptamine, the fluorescence intensity of the detection reagent changes significantly, exhibiting advantages such as high sensitivity, fast response time, and simple operation.

[0047] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A method for preparing a cross-linked porous polymer for detecting tryptophan, characterized in that, Monomer 1 and Monomer 2 were dissolved in an organic solvent at a predetermined ratio and stirred. Then, an alkaline additive was added, and the mixture was denitrified. Under nitrogen protection, CuI and tetra(triphenylphosphine)palladium catalysts were added, and the mixture was heated to 80-130°C and stirred for 48-72 hours for subsequent polymerization. After the reaction was completed, the mixture was cooled to room temperature, and the precipitated network polymer was filtered and washed successively with chloroform, water, methanol, and acetone. The polymer was then subjected to Soxhlet extraction with tetrahydrofuran and vacuum dried to obtain the cross-linked porous polymer. The Soxhlet extraction time was 72 hours. Monomer 1 is a BODIPY-type fluorescent component, and monomer 2 is tris(4-ethynylphenyl)amine. Monomer 1 and monomer 2 are polymerized through a Sonogashira coupling reaction, with a ratio of 1.5:

1. The organic solvent is toluene; The aforementioned BODIPY-type fluorescent component is characterized by having the following general structural formula: Wherein, R1 is one of phenyl, methyl, methoxy, ethyl, propyl, or CF3; and R2 is one of Br or I.

2. The method for preparing a cross-linked porous polymer for detecting tryptamine according to claim 1, characterized in that, The alkaline additive is one or more of triethylamine, diethylamine, and trimethylamine, and the ratio of organic solvent to alkaline additive is 4:

1.

3. The method for preparing a cross-linked porous polymer for detecting tryptamine according to claim 1, characterized in that, Toluene organic solvent was used, and the polymerization reaction temperature was 80-90℃ for 48-72 hours.

4. The method for preparing a cross-linked porous polymer for detecting tryptamine according to claim 1, characterized in that, The prepared cross-linked porous polymer contains both micropores and mesopores.

5. The application of the cross-linked porous polymer prepared by the method for preparing the cross-linked porous polymer for detecting tryptamine as described in claim 1, characterized in that, Cross-linked porous polymers dispersed in organic solvents form a detection reagent for the fluorescence sensing detection of α-methyltryptamine.

6. The application according to claim 5, characterized in that, The detection method for fluorescence sensing detection of α-methyltryptamine is performed according to the following steps: a) The obtained cross-linked porous polymer was added to the organic solvent methanol and ultrasonically dispersed to obtain a homogeneous detection reagent with a concentration of 0.1 mg / mL. The fluorescence emission spectrum under 480 nm excitation was then measured using a fluorescence spectrometer. b. Prepare a 1 mg / mL α-methyltryptamine methanol solution; c. Add different volumes of α-methyltryptamine methanol solution to the detection reagent obtained in step a in sequence. After each addition of α-methyltryptamine methanol solution, mix for 30 seconds and then measure the fluorescence emission spectrum under 480 nm excitation using a fluorescence spectrometer. d. Compare the changes in fluorescence intensity of the maximum emission peak in the fluorescence emission spectrum with the addition of different concentrations of the analyte, and calculate the key parameters of the detection limit.

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