PagWRKY40基因在调控杨树抗虫性中的应用

By overexpressing the PagWRKY40 gene in poplar trees and using Agrobacterium tumefaciens-mediated genetic transformation technology, transgenic poplar trees were constructed. This solved the problems of low efficiency in poplar pest control and the limitations of exogenous gene application in existing technologies, and achieved significant enhancement of insect resistance and improved activity of defense enzymes.

CN121653173BActive Publication Date: 2026-07-17JILIN AGRICULTURAL UNIV

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
JILIN AGRICULTURAL UNIV
Filing Date
2025-12-31
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

Existing technologies for controlling poplar pests suffer from low efficiency, high cost, environmental pollution, and significant impact on non-target organisms. The application of exogenous insect-resistant genes in poplars is limited, and pests are prone to developing resistance.

Method used

The insect resistance of poplar was improved by overexpressing the PagWRKY40 gene. Transgenic plants were constructed using Agrobacterium tumefaciens-mediated genetic transformation. The PagWRKY40 gene was used as a positive regulatory gene in response to insect pest stress to enhance the insect resistance of poplar.

Benefits of technology

The PagWRKY40 gene significantly enhances the insect resistance of poplar trees without affecting their growth, manifested in its antifeedant effect against the fall webworm and increased activity of defensive enzymes, thereby enhancing the plant's insect resistance.

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Abstract

PagWRKY40基因在调控杨树抗虫性中的应用,它涉及生物技术与基因工程领域,本发明的PagWRKY40基因或其所编码的蛋白质在调控杨树抗虫性中的应用,所述PagWRKY40基因的核苷酸序列如SEQ ID No.1所示。本发明通过克隆84K杨PagWRKY40基因,并利用根癌农杆菌介导的遗传转化方式,获得了该基因的过表达转基因植株。研究成果为杨树抗虫性品种改良提供了依据,对揭示林木基因工程育种的优良基因具有重要科学意义,84K杨PagWRKY40基因具有明显的抗虫能力,且不影响植物的生长,因此将该基因用于生长转基因植物尤其是转基因林木具有非常重要的应用前景。
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