A method for preparing and using a sensitive skin quick repair composition

Abstract

The application belongs to the technical field of cosmetic raw materials, and discloses a preparation method and application of a sensitive skin quick repair composition. The composition is prepared through the steps of golden chamomile extraction, white yam ultrasonic extraction, double-strain co-fermentation, and compound preservative emulsification. Ascorbic acid and sodium acetate are added in the middle of fermentation to form a "pH stability-enzyme activity activation-active ingredient protection" synergistic system. The composition has a total polyphenol content of 84.08 mg / g and a flavonoid content of 50.8 mg / g, and has significant anti-inflammatory soothing and barrier repair effects. The composition can be widely applied to skin care products such as water, emulsion and cream, and has high safety and good stability, and is suitable for sensitive skin quick first aid and long-term stability maintenance.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic raw material technology, specifically relating to a method for preparing a rapid repair composition for sensitive skin barrier repair, anti-inflammatory and soothing, and its application in cosmetics. Background Technology

[0002] In modern life, due to the combined effects of environmental pollution, ultraviolet radiation, mental stress, improper skincare practices, and frequent use of irritating cosmetics, sensitive skin has become a global health challenge. Sensitive skin is a skin condition characterized by abnormal barrier function, making it susceptible to adverse reactions to external stimuli. Its core issue lies in a weakened or damaged barrier function, accompanied by overactivation of the immune system and hyperreactivity of neurovascular systems, leading to a series of uncomfortable symptoms such as redness, burning, stinging, itching, dryness, and flaking, severely impacting the patient's quality of life.

[0003] Currently, most sensitive skin care products on the market use single or a few active ingredients, such as ceramides, hyaluronic acid, or plant extracts. While these can alleviate symptoms to some extent, they have the following significant drawbacks: (1) Limited target and limited repair: Most commercially available products use only one or a few active ingredients, such as simple ceramides, hyaluronic acid, or simple plant extracts. These formulations can only target a certain layer of the skin barrier and cannot solve the fundamental problem of a fragile barrier function. For example, although ingredients such as oligopeptide-1 can promote barrier repair, they have a single target and are difficult to address the multifactorial pathogenesis of sensitive skin. Although existing patents disclose a variety of sensitive skin repair compositions, most of them are simple ingredient stacking and lack synergistic effects.

[0004] (2) Unstable formulation system and significant efficacy decline: Plant extracts and fermentation products are prone to degradation, discoloration, and stratification in aqueous systems. Existing technologies have not solved the compatibility problem between high-content bio-fermentation products and oil-soluble plant extracts, resulting in short product shelf life and poor efficacy stability. Although some products claim to contain active ingredients, due to low actual transdermal efficiency, the active ingredients only remain in the epidermal layer and cannot act on the dermis to exert a deep repair effect.

[0005] (3) Insufficient safety verification and sensitization risk: People with sensitive skin have impaired skin barrier function, which significantly reduces their ability to defend against irritants and allergens. However, some commercially available products still have problems such as excessive microorganisms, heavy metal residues, or preservative irritation, which increases the risk of sensitization and irritation.

[0006] Golden chamomile extract is rich in anti-inflammatory active ingredients such as α-cineole, β-cineole, and chamomile lactone. Studies have shown that it can rapidly relieve vasodilation and neurogenic inflammation by inhibiting the NF-κB signaling pathway and reducing the release of pro-inflammatory factors such as IL-6 and TNF-α, resulting in a significant immediate soothing effect on sensitive skin. However, single plant extracts suffer from poor permeability and unstable activity, necessitating a complex repair ingredient that can work synergistically across multiple dimensions and possesses both high safety and stability to achieve rapid soothing, deep repair, and long-term stabilization of sensitive skin. Summary of the Invention

[0007] This invention addresses the shortcomings of existing sensitive skin repair compositions, which often contain low levels of flavonoids and polyphenols, resulting in slow repair of sensitive skin. The core objective of this invention is to provide a method for preparing a rapid sensitive skin repair composition that increases the content of active ingredients. This composition, when used in skincare products, offers high safety, good stability, and the ability to quickly repair sensitive skin and maintain long-term skin barrier stability.

[0008] This invention provides a method for preparing a rapid repair composition for sensitive skin, comprising the following steps: S1. Mix deionized water and dried chamomile powder in a mass ratio of 3-6:1 evenly, stir in a constant temperature water bath at 70-80℃ for 5-10 hours, filter after extraction, and rotary evaporate to obtain chamomile extract. S2. Add the powdered Bletilla striata tuber to a 30-50% ethanol solution at a material-to-liquid ratio of 1:(10-40), and extract with ultrasound at 50-80℃ for 1-3 hours. Filter to obtain Bletilla striata extract. S3. Mix chamomile extract and bletilla extract at a mass ratio of (1-5):1 to obtain a mixture. Add nitrogen source and inorganic salt to the mixture, adjust the pH to 5.0-6.5, sterilize for the first time, inoculate with brewer's yeast and Lactobacillus plantarum, ferment at 30-40℃ for 20-48 hours, add ascorbic acid and sodium acetate, continue fermentation for 30-72 hours, sterilize for the second time, homogenize and crush, filter to obtain fermentation filtrate; S4. Mix the fermentation filtrate, preservative and emulsifier from step S3 to obtain a rapid repair composition for sensitive skin.

[0009] Preferably, the rotary evaporation in step S1 involves concentrating the filtrate to 1 / 5 to 1 / 3 of its original volume by rotary evaporation at 50-60°C and -0.09 MPa.

[0010] Preferably, in step S3, the inoculation amounts of both *Saccharomyces cerevisiae* and *Lactobacillus plantarum* are 2-5% of the mixed liquid volume, and more preferably, the inoculation volume ratio of *Saccharomyces cerevisiae* to *Lactobacillus plantarum* is 1:1-3; the concentration of *Saccharomyces cerevisiae* and *Lactobacillus plantarum* is 1×10⁻⁶.9 ~1×10 11 CFU / mL.

[0011] Preferably, the concentration of ascorbic acid relative to the mixture is 0.1-1.0 g / kg, and the concentration of sodium acetate relative to the mixture is 0.5-2.0 g / kg.

[0012] Preferably, the nitrogen source is yeast extract and / or soybean peptone, and the amount added is 0.5%-2.0% of the total mass of the mixture; the inorganic salts include 0.1%-0.5% potassium dihydrogen phosphate and 0.05%-0.2% magnesium sulfate.

[0013] Preferably, in step S3, the homogenization and crushing is carried out using a high-pressure homogenizer with a homogenization pressure of 20-40 MPa and a homogenization frequency of 2-3 times.

[0014] Preferably, in step S3, the process parameters for the first sterilization and the second sterilization are 121℃, 0.1MPa, and sterilization time of 15-30min.

[0015] Preferably, in step S4, the mixing temperature is 35-45℃, the stirring speed is 100-150r / min, and the stirring time is 30-60min.

[0016] Preferably, in step S4, the preservative is at least one of 1,2-pentanediol, octanoyl hydroxamic acid, 1,3-propanediol, and butanediol, and the amount added is 0.3-0.8% of the mass of the fermentation filtrate; the emulsifier is at least one of PEG-40 hydrogenated castor oil and glyceryl stearate, and the amount added is 1-3.5% of the mass of the fermentation filtrate.

[0017] The present invention also provides a composition for rapid repair of sensitive skin, prepared according to the method described in the present invention.

[0018] Preferably, the present invention also provides the application of the aforementioned composition for rapid repair of sensitive skin in the preparation of skin care products.

[0019] Preferably, the present invention also provides a skin care product containing 1-50% of the aforementioned sensitive skin rapid repair composition.

[0020] Preferably, the skin care product formulation is selected from at least one of aqueous solution, lotion, cream, serum, or mask liquid.

[0021] Preferably, the present invention also provides an emulsion for rapid repair of sensitive skin, comprising 10-30% of a rapid repair composition for sensitive skin, 8-20% of a moisturizer, 1-5% of a skin barrier repair agent, 3-10% of a mild oil, 0.1-0.5% of a thickener, 1.5-4.5% of an emulsifier, and the balance being deionized water.

[0022] Preferably, the moisturizer is at least one of glycerin, sodium hyaluronate, and panthenol; the skin barrier repair agent is at least one of ceramide, cholesterol, and phytosterol; the mild oil is at least one of squalane and jojoba oil; the thickener is at least one of xanthan gum, carbomer, hydroxyethyl cellulose, and guar gum; and the emulsifier is at least one of hydrogenated lecithin, cetearyl oleate, and sorbitan oleate.

[0023] This invention involves adding ascorbic acid to the fermentation system 20-48 hours after inoculation with *Saccharomyces cerevisiae* and *Lactobacillus plantarum*, significantly increasing the content of flavonoids and polyphenols in the fermentation broth. Ascorbic acid maintains a reducing environment in the fermentation system, activating key enzymes in the synthesis and conversion of flavonoids and polyphenols, such as β-glucosidase and phenylalanine ammonia-lyase, promoting the directed hydrolysis of bound flavonoid glycosides and polyphenolic glycosides in chamomile extract into free active components. Furthermore, it facilitates the scavenging of free radicals generated during fermentation through strong oxidation, inhibiting structural damage and activity loss of flavonoids and polyphenols due to oxidation, reducing the loss of active components in subsequent processes, and achieving efficient synthesis and retention of flavonoids and polyphenols. In the mid-fermentation stage, the strain has entered the late logarithmic growth phase or the secondary metabolic stage, where oxidative stress begins to intensify; adding ascorbic acid at this point effectively protects the active components.

[0024] During the mid-fermentation stage (20-48 hours), the pH of the fermentation broth gradually decreases. The added sodium acetate, as a weak acid salt, effectively stabilizes the pH of the fermentation system within the optimal activity range of 5.5-6.5 for flavonoid synthases. Simultaneously, it can specifically activate the activity of phenylalanine ammonia-lyase, promoting the targeted synthesis of flavonoids. Furthermore, sodium acetate and ascorbic acid form a synergistic system of "pH stabilization - enzyme activity activation - active ingredient protection," further significantly increasing the content of flavonoids and polyphenols in the fermentation broth.

[0025] This invention utilizes a co-fermentation technique involving golden chamomile extract and Bletilla striata extract to successfully prepare a safe and highly effective rapid repair composition for sensitive skin. This process achieves structural transformation and efficient enrichment of plant active ingredients. The raw material is non-toxic to skin cells, rapidly promotes keratinocyte proliferation and migration, inhibits the release of inflammatory factors, and improves mitochondrial function, thus quickly repairing symptoms of sensitive skin discomfort. It is suitable for various sensitive skin repair cosmetics and has broad market application prospects. Specific features are as follows: (1) Anti-inflammatory and regulatory effects: α-bisabolol in chamomile and bibenzyl compounds in Bletilla striata jointly inhibit the activation of the NF-κB signaling pathway, reduce the release of pro-inflammatory factors such as IL-1β, IL-8, and TNF-α; at the same time, they balance the Th1 / Th2 immune response, reduce mast cell degranulation, inhibit the excessive activation of the immune system, and alleviate the inflammatory response from the source.

[0026] (2) Barrier repair efficacy: The polysaccharides of different molecular weights produced during co-fermentation form a targeted action system - macromolecular polysaccharides (>100kDa) form a physical protective film on the cell surface to achieve immediate moisturization; medium molecular weight polysaccharides (10-100kDa) strengthen the intercellular connections in the superficial epidermis; small molecular weight polysaccharides (<10kDa) penetrate to the basal layer, regulate intracellular functions, promote ceramide synthesis, and enhance the hydration capacity of the stratum corneum. At the same time, Bletilla striata polysaccharide and Chamomile polysaccharide work synergistically to reduce the activity of barrier-damaging enzymes (such as matrix metalloproteinases) and stabilize the skin barrier.

[0027] (3) Neurovascular stabilizing effect: The volatile oil components and fermentation products in chamomile work together to block the neurogenic inflammatory pathway, stabilize vascular endothelial cells, and reduce vasodilation and increased permeability; at the same time, the antioxidant components protect nerve cells, reduce neurovascular hyperreactivity, and relieve skin redness and burning sensation.

[0028] (4) Improvement of mitochondrial function: The active ingredients in the co-fermentation products (such as flavonoid derivatives) can promote the expression of mitochondrial oxidative phosphorylation-related proteins, enhance the stability of mitochondrial membrane potential, improve ATP production efficiency, provide energy for keratinocyte proliferation, migration and lipid synthesis, and accelerate the skin repair process.

[0029] The advantages or beneficial effects of the composition for rapid repair of sensitive skin of the present invention include at least the following: This invention utilizes a co-fermentation technology combining golden chamomile and Bletilla striata extracts to achieve structural transformation and efficient enrichment of active ingredients, resulting in a total polyphenol content of 84.08 mg / g and a total flavonoid content of 50.8 mg / g. This high concentration of active ingredients can rapidly repair sensitive skin. The core of the co-fermentation technology lies in the addition of sodium acetate and ascorbic acid during the mid-fermentation of Lactobacillus plantarum and Saccharomyces cerevisiae, forming a synergistic system of "pH stabilization - enzyme activity activation - active ingredient protection." This increases the content of flavonoids and polyphenols in the fermentation broth, achieving inhibition rates of 48.1-62.3% and 45.2-65.8% against IL-6 and TNF-α, respectively, and a keratinocyte proliferation rate of 135.3-168.5%. It not only possesses excellent antioxidant and anti-inflammatory activities but also achieves rapid repair of sensitive skin by improving skin barrier function and promoting cell proliferation and migration. Attached Figure Description

[0030] Figure 1 Microscopic image showing the effect of the composition for rapid repair of sensitive skin in Example 1 on cell migration ability.

[0031] Figure 2 The effect of the composition for rapid repair of sensitive skin in Example 1 on the migration ability of HaCaT cells.

[0032] Figure 3 The effect of the composition for rapid repair of sensitive skin in Example 1 on the expression levels of genes related to redness, swelling, itching, heat and pain in HUVEC cells. Detailed Implementation

[0033] To more clearly illustrate the purpose, technical solution, and advantages of this invention, the technical solution of this invention will be described in detail below through specific embodiments. It should be noted that these embodiments are only for illustrating this invention and not for limiting its scope of protection; the actual scope of protection of this invention should be determined by the claims.

[0034] Unless otherwise specified, the materials and reagents used in the following examples and comparative examples are commercially available. Unless otherwise specified, the amount of each component in the following examples is 1 g per part by weight.

[0035] I. Experimental Materials: Saccharomyces cerevisiae, accession number: CCTCC AY 2019005, purchased from China Center for Type Culture Collection.

[0036] Lactobacillus plantarum, accession number: CCTCC AB 2022276, purchased from China Center for Type Culture Collection.

[0037] All other materials are commercially available products.

[0038] II. Examples and Comparative Examples of Rapid Repair Compositions for Sensitive Skin Example 1 S1: Mix deionized water and dried chamomile powder at a mass ratio of 3:1, stir in a constant temperature water bath at 70℃ for 5 hours, filter, and then concentrate to 1 / 5 of the original volume by rotary evaporation at 50℃ and -0.09MPa to obtain chamomile extract.

[0039] S2: Add Bletilla striata tuber powder to 30% ethanol solution at a material-to-liquid ratio of 1:10, extract by ultrasonication at 50℃ for 1 hour, and filter to obtain Bletilla striata extract.

[0040] S3: Mix chamomile extract and Bletilla striata extract at a mass ratio of 1:1. Add 0.5% yeast extract (by mass of the total mixture), 0.1% potassium dihydrogen phosphate, and 0.05% magnesium sulfate. Adjust the pH to 5.0 and sterilize at 121℃ and 0.1MPa for 15 minutes. Inoculate with 2% volume of Saccharomyces cerevisiae and 2% volume of Lactobacillus plantarum (both at a concentration of 1×10⁻⁶). 10 Fermentation was carried out at 30℃ for 20 hours (CFU / mL). Ascorbic acid (0.1 g / kg) and sodium acetate (0.5 g / kg) were added, and fermentation continued for another 30 hours, followed by a second sterilization for 15 minutes. The mixture was then homogenized twice under high pressure at 30 MPa, and the fermentation filtrate was obtained by filtration.

[0041] S4: Add 0.3% 1,2-pentanediol and 1% PEG-40 hydrogenated castor oil to the fermentation filtrate, and stir at 35°C and 100 r / min for 30 min to obtain the composition.

[0042] Example 2 S1: Mix deionized water and dried chamomile powder at a mass ratio of 4:1, stir in a constant temperature water bath at 75℃ for 7 hours, filter, and then concentrate to 1 / 4 of the original volume by rotary evaporation at 55℃ and -0.09MPa to obtain chamomile extract.

[0043] S2: Add Bletilla striata tuber powder to 35% ethanol solution at a material-to-liquid ratio of 1:20, extract by ultrasonication at 60℃ for 2 hours, and filter to obtain Bletilla striata extract.

[0044] S3: Mix the extract at a mass ratio of 2:1, add 1.0% soybean peptone, 0.2% potassium dihydrogen phosphate, and 0.1% magnesium sulfate, adjust the pH to 5.5, and sterilize for 20 min. Inoculate with 3% volume of Saccharomyces cerevisiae and 4% volume of Lactobacillus plantarum (concentration 1×10⁻⁶). 1 Fermentation was carried out at 35℃ for 30 hours (CFU / mL). Ascorbic acid (0.3 g / kg) and sodium acetate (1.0 g / kg) were added, and fermentation continued for 48 hours, followed by a second sterilization for 20 minutes. The mixture was then homogenized twice under high pressure at 25 MPa, and the fermentation filtrate was obtained by filtration.0 S4: Add 0.5% capryloyl hydroxamic acid and 2% glyceryl stearate, stir at 40°C and 120 r / min for 40 min to obtain the composition.

[0045] Example 3 S1: Mix deionized water and dried chamomile powder at a mass ratio of 5:1, stir in a constant temperature water bath at 75℃ for 8 hours, filter, and then concentrate by rotary evaporation at 55℃ and -0.09MPa to 1 / 3 of the original volume to obtain chamomile extract.

[0046] S2: Add Bletilla striata tuber powder to 40% ethanol solution at a material-to-liquid ratio of 1:30, extract by ultrasonication at 70℃ for 2 hours, and filter to obtain Bletilla striata extract.

[0047] S3: Mix chamomile extract and Bletilla striata extract at a mass ratio of 3:1. Add 1.5% of the total mass of the mixture of yeast extract and soybean peptone (mass ratio 1:1), 0.3% potassium dihydrogen phosphate, and 0.15% magnesium sulfate. Adjust the pH to 6.0 and sterilize at 121℃ and 0.1MPa for 25 min. Inoculate with 4% volume of Saccharomyces cerevisiae and 3% volume of Lactobacillus plantarum (both at a concentration of 1×10⁻⁶). 10 Fermentation was carried out at 35℃ for 36 hours (CFU / mL). Ascorbic acid (0.5 g / kg) and sodium acetate (1.2 g / kg) were added, and fermentation continued for 50 hours, followed by a second sterilization for 25 minutes. The mixture was homogenized three times under high pressure at 30 MPa and filtered to obtain the fermentation filtrate.

[0048] S4: Add 0.6% of 1,2-pentanediol and octanoyl hydroxamic acid (mass ratio 1:1) and 2.5% of PEG-40 hydrogenated castor oil and glyceryl stearate (mass ratio 1:1) to the fermentation filtrate, and stir at 40°C and 130 r / min for 45 min to obtain the composition.

[0049] Example 4 S1: Mix deionized water and dried chamomile powder at a mass ratio of 5:1, stir in a constant temperature water bath at 78℃ for 9 hours, filter, and then concentrate by rotary evaporation at 58℃ and -0.09MPa to 1 / 4 of the original volume to obtain chamomile extract.

[0050] S2: Add Bletilla striata tuber powder to 45% ethanol solution at a material-to-liquid ratio of 1:35, extract by ultrasonication at 75℃ for 2.5h, and filter to obtain Bletilla striata extract.

[0051] S3: Mix the extract at a mass ratio of 4:1, add 1.8% soybean peptone, 0.4% potassium dihydrogen phosphate, and 0.18% magnesium sulfate, adjust the pH to 6.2, and sterilize for 28 min. Inoculate with 2.5% volume of dual-strain culture (concentration 1×10⁻⁶). 11 Fermentation was carried out at 38℃ for 42 hours (CFU / mL). Ascorbic acid (0.8 g / kg) and sodium acetate (1.8 g / kg) were added, and fermentation continued for 60 hours, followed by a second sterilization for 28 minutes. The mixture was then homogenized three times under high pressure at 35 MPa and filtered to obtain the fermentation filtrate.

[0052] S4: Add 0.7% capryloyl hydroxamic acid and 3% glyceryl stearate, stir at 42°C and 140 r / min for 50 min to obtain the composition.

[0053] Example 5 S1: Mix deionized water and dried chamomile powder at a mass ratio of 6:1, stir in a constant temperature water bath at 80℃ for 10 hours, filter, and then concentrate to 1 / 3 of the original volume by rotary evaporation at 60℃ and -0.09MPa to obtain chamomile extract.

[0054] S2: Add 50% ethanol solution to Bletilla striata tuber powder at a material-to-liquid ratio of 1:40, extract by ultrasonication at 80℃ for 3 hours, and filter to obtain Bletilla striata extract.

[0055] S3: Mix the extract at a mass ratio of 5:1, add 2.0% yeast extract powder (by mass of the total mixture), 0.5% potassium dihydrogen phosphate, and 0.2% magnesium sulfate, adjust the pH to 6.5, and sterilize for 30 min. Inoculate with 5% volume of dual-strain culture (concentration 1×10⁻⁶). 9 Fermentation was carried out at 40℃ for 48 hours (CFU / mL). Ascorbic acid (1.0 g / kg) and sodium acetate (2.0 g / kg) were added, and fermentation continued for 72 hours, followed by a second sterilization for 30 minutes. The mixture was then homogenized three times under high pressure at 40 MPa and filtered to obtain the fermentation filtrate.

[0056] S4: Add 0.8% of 1,3-propanediol and butylene glycol (mass ratio 1:1) and 3.5% of PEG-40 hydrogenated castor oil, and stir at 45°C and 150 r / min for 60 min to obtain the composition.

[0057] Comparative Example 1 Step S3 did not involve the addition of ascorbic acid; the remaining steps were the same as in Example 1.

[0058] Comparative Example 2 In step S3, sodium acetate was not added; the remaining steps were the same as in Example 1.

[0059] Comparative Example 3 Use an equal amount of Bletilla striata extract instead of chamomile extract, otherwise remain the same as in Example 1.

[0060] Comparative Example 4 Use an equal amount of chamomile extract instead of Bletilla striata extract, otherwise remain the same as in Example 1.

[0061] Comparative Example 5 Step S3 involves inoculating only 4% of the volume of brewer's yeast, with the rest being the same as in Example 1.

[0062] Comparative Example 6 Step S3 involves inoculating only 4% of the volume of Lactobacillus plantarum, with the rest being the same as in Example 1.

[0063] III. Performance Characterization of the Rapid Repair Composition for Sensitive Skin 1. Determination of active ingredient content in fermentation broth Flavonoid content: determined by ultraviolet spectrophotometry (AlCl3 colorimetric method).

[0064] Polyphenol content: determined by the Folin-phenol colorimetric method.

[0065] 2. Soothing and anti-inflammatory efficacy test The inhibition rate of IL-6 and TNF-α secretion by samples was detected using an LPS-induced HaCaT cell model. Specifically, HaCaT cells were collected by centrifugation and the cell density was adjusted to 2.5 × 10⁻⁶ cells / year. 5 The drug was seeded in 12-well plates at a concentration of 200 ng / mL and cultured overnight in a cell culture incubator. After HaCaT cells adhered, the drug was diluted with serum-free basal medium and the stimulant LPS was added to a final concentration of 200 ng / mL. After culturing for 6 h, RNA was extracted and the expression level of inflammatory factor mRNA in HaCaT cells was detected by qPCR.

[0066] Keratinocyte proliferation rate: The specific method is as follows: HaCaT cells in good growth condition are seeded into 96-well plates and cultured for 17 h. After changing to serum-free basal medium and culturing for 7 h, the old medium is discarded and basal medium containing different concentrations of the test samples is added. After 48 h, OD450nm and OD630nm are detected by CCK8 method to obtain the effect of the test samples on HaCaT cell proliferation.

[0067] Table 1: Performance test results of the examples and comparative examples are shown in the table below:

[0068] As shown in Table 1, the rapid skin repair compositions of the examples have high contents of flavonoids and polyphenols. Specifically, Example 3 has a total polyphenol content of 84.08 mg / g and a flavonoid content of 50.8 mg / g, with significantly increased active ingredient content, far exceeding that of the comparative examples. This is because the ascorbic acid added during the fermentation process forms a synergistic system with sodium acetate, activating the activity of key enzymes in flavonoid and polysaccharide synthesis while inhibiting the oxidative degradation of active ingredients. The examples also exhibit stronger antioxidant and anti-inflammatory activities, with inhibition rates of 48.1-62.3% and 45.2-65.8% against IL-6 and TNF-α, respectively, and a keratinocyte proliferation rate of 135.3-168.5%, demonstrating excellent anti-inflammatory and barrier repair capabilities. By improving skin barrier function and promoting cell proliferation and migration, they achieve rapid repair of sensitive skin.

[0069] Comparative Examples 1-2 used only ascorbic acid or sodium acetate, which could not form a synergistic system of "pH stability - enzyme activity activation - active ingredient protection", resulting in lower content of flavonoids and polyphenols in the fermentation broth than in Example 1, thus reducing the inhibition rate of IL-6 and TNF-α and the proliferation rate of keratinocytes.

[0070] Comparative Examples 3-4 used only either golden chamomile extract or Bletilla striata extract, without using the co-fermentation technology of both. This prevented the formation of α-bisabolol from chamomile and bibenzyl compounds from Bletilla striata to jointly inhibit the activation of the NF-κB signaling pathway, resulting in the release of pro-inflammatory factors such as IL-1β, IL-8, and TNF-α in the fermentation broth and a decrease in the inhibition rate.

[0071] Comparative Example 5 only used Saccharomyces cerevisiae for co-fermentation, lacking the β-glucosidase produced by Lactobacillus plantarum fermentation to hydrolyze chamomile flavonoids into free aglycones; Comparative Example 6 only used Lactobacillus plantarum for co-fermentation, and the content of flavonoids and polyphenols in the fermentation filtrate was lower than that in Example 1, resulting in decreased anti-inflammatory activity, decreased skin barrier function and cell proliferation and migration, and failure to achieve rapid repair of sensitive skin.

[0072] IV. Efficacy characterization of the rapid repair composition for sensitive skin.

[0073] (1) Effects on HaCaT cell migration ability (I) Experimental conditions: Cell line: HaCaT cells Density: 3*10 5 / ml Tested Sample: Example 1 Sensitive Skin Rapid Repair Composition Positive control: 8% FBS (II) Experimental method: HaCaT cells in the logarithmic growth phase were seeded into 12-well plates. After 24 hours, when the cells had filled the bottom, the bottom was gently cut with a 200 μL pipette tip to simulate human skin injury. The culture medium was aspirated and the detached cells were gently rinsed with PBS. Then, culture medium containing different concentrations of the test sample was added. A blank control group and a positive control group were set up. Microscopic images were taken at 0 h, 12 h and 24 h. The scar area was then statistically analyzed and the healing rate was calculated using ImageJ software.

[0074] *: P < 0.05 compared to the control group; **: P < 0.01 compared to the control group; ***: P < 0.001 compared to the control group; ***: P < 0.0001 compared to the control group.

[0075] Results analysis: Figure 1-2 The results showed that the cell scratch healing area of ​​the tested samples was significantly increased at 12h and 24h after scratching, which was significantly different from that of the control group.

[0076] (2) Expression levels of genes related to redness, swelling, itching, heat and pain in HUVEC cells by the tested samples (I) Experimental conditions: Tested Sample: Example 1 Sensitive Skin Rapid Repair Composition Positive control: 10 μM dexamethasone (II) Experimental methods: HUVEC cells were collected and seeded in 12-well plates. After the cells adhered, they were cultured in complete culture medium of different concentrations of the test samples for 6 hours. Then, they were co-stimulated with LPS (10 μg / mL) for 24 hours. RNA was extracted and the effects of qPCR on the expression of genes related to redness, swelling, itching, heat and pain in HUVEC cells were detected.

[0077] *: P < 0.05 compared to the model group; **: P < 0.01 compared to the model group; ***: P < 0.001 compared to the model group; ***: P < 0.0001 compared to the model group.

[0078] #: P < 0.05 compared to the control group; ##: P < 0.01 compared to the control group; ###: P < 0.001 compared to the control group; ####: P < 0.0001 compared to the control group.

[0079] Experimental results: Figure 3 The results showed that, compared with the blank group, the expression of ET-1, TNF-α, and IL-6 genes was significantly increased in the model group. Compared with the model group, the concentration of sensitive skin emergency repair raw materials between 0.125% and 0.5% had a certain inhibitory effect on the gene expression of ET-1, TNF-α, and IL-6, which was significantly different from that in the model group and significantly higher than that in the blank control group.

[0080] V. Application of Rapid Repair Compositions for Sensitive Skin in Cosmetics 1. Application Example 1 A skin lotion, composed of the following ingredients in parts by weight: Example 1: Rapid Repair Composition for Sensitive Skin (20%) Sodium hyaluronate: 5% Ceramide NP: 1% Squalane: 3% Jojoba oil: 2% Glycerin: 10% PEG-40 hydrogenated castor oil: 2% Xanthan gum: 0.3% 1,2-Pentanediol: 0.5% Deionized water: Balance.

[0081] The preparation method for skin lotion is as follows: S1. Preparation of aqueous phase: Dissolve the sensitive skin rapid repair composition, sodium hyaluronate, ceramide NP, glycerin, and 1,2-pentanediol in deionized water; heat to 70-80℃ and stir evenly to obtain the aqueous phase.

[0082] S2. Preparation of oil phase: Squalane, jojoba oil and PEG-40 hydrogenated castor oil are mixed, heated to 60-70℃ and stirred evenly to obtain the oil phase.

[0083] S3. Emulsification and homogenization: Slowly add the oil phase to the aqueous phase while homogenizing and mixing; perform high-speed shearing at 10000r / min for 1-3min, followed by high-pressure homogenization; when the temperature drops to 40-45℃, add xanthan gum thickener and continue stirring for 30-40min, then discharge to obtain the skin care emulsion.

[0084] Application Example 2 A skin lotion, composed of the following ingredients in parts by weight: Example 2: Rapid Repair Composition for Sensitive Skin (15%) Glycerin: 15% Jojoba oil: 5% Ceramide NP: 1% PEG-40 hydrogenated castor oil: 2% Xanthan gum: 0.3% 1,2-Pentanediol: 0.5% Deionized water: Balance.

[0085] The preparation method is the same as in Example 1.

[0086] Application Example 3 A skin lotion, composed of the following ingredients in parts by weight: Example 2: Rapid Repair Composition for Sensitive Skin (30%) Panthenol: 5% Phytosterols: 2% Squalane: 3% Glycerin: 10% PEG-40 hydrogenated castor oil: 2% Xanthan gum: 0.3% 1,2-Pentanediol: 0.5% Deionized water: Balance.

[0087] The preparation method is the same as in Example 1.

[0088] 2. Stability Test (1) High temperature stability test: The emulsion was put into a glass bottle, sealed and placed in a constant temperature drying oven and stored at 45°C for 3 months. The emulsion was observed weekly to see if it separated, separated or changed color.

[0089] Test results: The emulsions in Application Examples 1-3 showed no stratification, precipitation, or discoloration, and the viscosity change was ≤8%, meeting the stability requirements.

[0090] (2) Low temperature stability test: The emulsion was put into a glass bottle, sealed and refrigerated in a refrigerator at 1-3℃ for 3 months. The emulsion was observed weekly to see if it separated, separated or changed color.

[0091] Test Results: The emulsions in Application Examples 1-3 showed no stratification, precipitation, or discoloration, and their fluidity returned to normal after returning to room temperature. The above examples are merely illustrative of the invention and are not intended to limit the possible implementations of the invention. Based on the disclosure of this invention, those skilled in the art can make various modifications and adjustments. It is neither possible nor necessary to list all possible implementations. Any modifications, equivalent substitutions, or improvements made within the basic principles and scope of this invention should be considered within the protection scope of this invention.

Claims

1. A method for preparing a rapid repair composition for sensitive skin, characterized in that, Includes the following steps: S1. Mix deionized water and dried chamomile powder in a mass ratio of 3-6:1 evenly, stir in a constant temperature water bath at 70-80℃ for 5-10 hours, filter after extraction, and rotary evaporate to obtain chamomile extract. S2. Add the powdered Bletilla striata tuber to a 30-50% ethanol solution at a material-to-liquid ratio of 1:(10-40), and extract with ultrasound at 50-80℃ for 1-3 hours. Filter to obtain Bletilla striata extract. S3. Mix chamomile extract and bletilla striata extract at a mass ratio of (1-5):1 to obtain a mixture. Add nitrogen source and inorganic salt to the mixture, adjust the pH to 5.0-6.5, sterilize for the first time, inoculate with brewer's yeast and lactobacillus plantarum, ferment at 30-40℃ for 20-48 hours, add ascorbic acid and sodium acetate, continue fermentation for 30-72 hours, sterilize for the second time, homogenize and crush, filter to obtain fermentation filtrate; the concentration of ascorbic acid relative to the mixture is 0.1-1.0 g / kg, and the concentration of sodium acetate relative to the mixture is 0.5-2.0 g / kg. S4. Mix the fermentation filtrate, preservative and emulsifier from step S3 to obtain a rapid repair composition for sensitive skin. In step S3, the inoculation amount of both *Saccharomyces cerevisiae* and *Lactobacillus plantarum* is 2-5% of the mixed liquid volume, and the inoculation volume ratio of *Saccharomyces cerevisiae* to *Lactobacillus plantarum* is 1:1-3; the concentration of *Saccharomyces cerevisiae* and *Lactobacillus plantarum* is 1×10⁻ 9 ~1×10 11 CFU / mL; The rotary evaporation in step S1 involves concentrating the filtrate to 1 / 5 to 1 / 3 of its original volume at 50-60°C and -0.09 MPa.

2. The method for preparing the rapid repair composition for sensitive skin as described in claim 1, characterized in that, The nitrogen source is yeast extract and / or soybean peptone, and the amount added is 0.5%-2.0% of the total mass of the mixture; the inorganic salts include 0.1%-0.5% potassium dihydrogen phosphate and 0.05%-0.2% magnesium sulfate.

3. The method for preparing a rapid repair composition for sensitive skin as described in any one of claims 1-2, characterized in that, In step S3, the homogenization and crushing are performed using a high-pressure homogenizer with a homogenization pressure of 20-40 MPa and a homogenization frequency of 2-3 times. In step S3, the process parameters for the first and second sterilizations are 121℃ and 0.1 MPa, with a sterilization time of 15-30 min. In step S4, the mixing temperature is 35-45℃, the stirring speed is 100-150 r / min, and the stirring time is 30-60 min. In step S4, the preservative is at least one of 1,2-pentanediol, octanoyl hydroxamic acid, 1,3-propanediol, and butylene glycol, added at a rate of 0.3-0.8% of the fermentation filtrate mass. The emulsifier is at least one of PEG-40 hydrogenated castor oil and glyceryl stearate, added at a rate of 1-3.5% of the fermentation filtrate mass.

4. A composition for rapid repair of sensitive skin, characterized in that, The composition is prepared by any one of the preparation methods of claims 1-3.

5. A skincare product, characterized in that, The skincare product contains 1-50% of the composition for rapid repair of sensitive skin as described in claim 4; the skincare product dosage form is selected from at least one of aqueous solution, lotion, cream, serum or mask liquid.

6. A lotion for rapid repair of sensitive skin, characterized in that, The composition comprises 10-30% of the sensitive skin rapid repair composition as described in claim 4, 8-20% of the moisturizer, 1-5% of the skin barrier repair agent, 3-10% of the mild oil, 0.1-0.5% of the thickener, 1.5-4.5% of the emulsifier, and the balance being deionized water.

7. A lotion for rapid repair of sensitive skin as described in claim 6, characterized in that, The moisturizer is at least one of glycerin, sodium hyaluronate and panthenol; the skin barrier repair agent is at least one of ceramide, cholesterol and phytosterol; the mild oil is at least one of squalane and jojoba oil; the thickener is at least one of xanthan gum, carbomer, hydroxyethyl cellulose and guar gum; and the emulsifier is at least one of hydrogenated lecithin, cetearyl oleate and sorbitan oleate.

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