一种高粱全基因组液相芯片及其构建方法与应用

By constructing a sorghum whole-genome liquid-phase chip containing 5446 SNPs and 3 InDel molecular markers, the problem of high resequencing costs in sorghum breeding was solved, enabling rapid and accurate screening of breeding materials and variety identification.

CN121737333BActive Publication Date: 2026-07-17JILIN ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
JILIN ACAD OF AGRI SCI
Filing Date
2025-12-24
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

Whole-genome resequencing in sorghum breeding is expensive and difficult, making it unaffordable for breeding units, and there is a lack of rapid and accurate genotype screening tools.

Method used

A sorghum whole-genome liquid microarray was developed, containing 5446 SNP molecular markers and 3 InDel molecular markers. Through whole-genome resequencing, data filtering, and probe design, the sorghum whole-genome liquid microarray was constructed for sorghum variety identification, kinship identification, genetic diversity analysis, genetic map construction, gene localization and cloning, and molecular marker-assisted selection.

Benefits of technology

It reduces the cost of sorghum breeding, provides a rapid and accurate means of screening breeding materials, and supports the identification and breeding process of sorghum varieties.

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Abstract

本发明涉及基因芯片技术领域,尤其涉及一种高粱全基因组液相芯片及其构建方法与应用。本发明的高粱全基因组液相芯片的检测靶点由5446个SNP分子标记和3个InDel分子标记组成;所述5446个SNP分子标记和3个InDel分子标记位于高粱参考基因组Sorghum_bicolor_v3.1.1上的位置如表1所示;本发明的高粱全基因组液相芯片可用于高粱品种鉴定、亲缘关系鉴定、遗传多样性分析、分子遗传图谱构建、基因定位和克隆、分子标记辅助选择、全基因组选择育种,为高粱重要农艺性状解析和生物育种提供重要分子依据。
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Citation Information

Patent Citations

  • Sorghum high-density whole-genome SNP chip and application thereof

    CN118792298A