A targeted light-weight spectral library construction system for high-sensitivity single-cell proteomics research and application thereof

Abstract

This invention belongs to the fields of bioinformatics and proteomics, specifically relating to a targeted, lightweight spectral library construction system for high-sensitivity single-cell proteomics research and its applications. Based on single-cell DIA data, this system achieves precise construction and redundancy control of the spectral library through high-confidence peptide extraction, priority import of functional proteins, target-guided spectral deduction, expression score-driven entry compression, and structural noise reduction optimization. This method significantly improves the protein recognition rate and reproducibility of single-cell DIA data, particularly excelling in the identification of functional proteins such as membrane receptors, kinases, transcription factors, and ubiquitin-regulated proteins. This invention can also be applied to the identification of key functional proteins in various immune cells and the identification of tumor-associated macrophage subsets, providing efficient and reliable technical support for functional analysis and subset classification at the single-cell proteomics level, and possesses good versatility and promotional value.

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