Method for planting dendrobium candidum on epiphyte of magnolia grandiflora

Abstract

The application discloses a method for planting Dendrobium candidum on Magnolia grandiflora, and belongs to the technical field of Dendrobium candidum epiphytic planting, and specifically comprises the following steps: (1) epiphytic tree treatment: determining a planting point on a trunk, cutting off the bark at the planting point to obtain a planting window; (2) Dendrobium candidum seedling preparation: cleaning Dendrobium candidum seedlings, and reserving the seedlings after disinfection; (3) Dendrobium candidum planting: firstly, applying a layer of protective gel on the planting window of the epiphytic tree, then closely attaching a planting base layer to the surface of the protective gel, and finally closely planting the Dendrobium candidum seedlings on the planting base layer, uniformly wrapping the seedlings with moss, ensuring that the roots of the seedlings are completely covered with the moss, and firmly binding the roots with a grass rope; and (4) later management: spraying water to keep the roots of the Dendrobium candidum seedlings moist after planting. The method solves the problem of low survival rate of Dendrobium candidum on Magnolia grandiflora.

Description

Technical Field

[0001] This invention relates to the field of epiphytic cultivation technology of Dendrobium officinale, and in particular to a method for epiphytic cultivation of Dendrobium officinale using Magnolia grandiflora. Background Technology

[0002] Dendrobium officinale Kimura et Migo is a perennial epiphytic herb belonging to the genus Dendrobium in the Orchidaceae family. It blooms from May to July with pale yellow flowers, its small and elegant form making it highly ornamental. The delicate fragrance of Dendrobium officinale has a calming and soothing effect. It thrives in warm, humid, and semi-shaded environments and can grow epiphytically on living trees. Magnolia grandiflora is an evergreen tree in the Magnoliaceae family. It has a large crown, thick, glossy leaves, large, fragrant flowers, and a majestic appearance, making it a common landscape tree. In addition to its strong air-purifying capabilities, the active ingredients in its buds and bark can effectively relieve symptoms of colds and flu. Magnolia grandiflora blooms from May to July with pure white flowers that bloom at the top of branches, making its upper layer highly ornamental. However, the lower layer has a monotonous structure and color. Therefore, Dendrobium officinale can be epiphyted on Magnolia grandiflora trees. This not only increases the spatial layering and color richness of the Magnolia grandiflora, enhancing its ornamental value, but also allows the rich fragrance of the Magnolia grandiflora to intertwine with the elegant fragrance of Dendrobium officinale, thus enhancing the therapeutic effect of the landscape space.

[0003] Magnolia grandiflora has thin, smooth bark, making it difficult to accumulate humus on the trunk surface. Direct epiphytic planting of Dendrobium officinale results in a low survival rate. Therefore, a cultivation substrate that can accumulate humus and moisture and promote Dendrobium officinale growth needs to be added between the Dendrobium officinale and the Magnolia grandiflora trunk. Existing techniques include cutting planting trenches of a certain depth in the trunk and placing the cultivation substrate in the trenches to plant the Dendrobium. However, the thin bark of Magnolia grandiflora means that setting up planting trenches would penetrate deep into the xylem, affecting the survival of the tree. Alternatively, the cultivation substrate can be tied to the trunk for planting. However, the smooth bark of Magnolia grandiflora results in poor adhesion to the substrate. Under prolonged exposure to wind and rain, and the increasing gravity as the Dendrobium grows, the substrate easily detaches from the trunk, leading to insufficient nutrient absorption and eventual wilting and death of the Dendrobium. Therefore, there is an urgent need for a new epiphytic cultivation method for Dendrobium officinale, which can enable the substrate to bond firmly with the smooth bark of Magnolia grandiflora, continuously provide nutrients and water for Dendrobium officinale, and promote its long-term survival. Summary of the Invention

[0004] Therefore, the purpose of this invention is to provide a method for epiphytic cultivation of Dendrobium officinale on Magnolia grandiflora, which solves the problem that the cultivation substrate is not firmly bonded to the trunk of Magnolia grandiflora and is easy to fall off, thus affecting the survival of Dendrobium officinale.

[0005] The present invention solves the above-mentioned technical problems through the following technical means:

[0006] (1) Treatment of epiphytic trees: Select healthy magnolia trees with a diameter at breast height greater than 15cm as epiphytic trees, determine the planting point on the trunk, and remove the bark at the planting point to the depth of just touching the xylem to obtain the planting window;

[0007] (2) Dendrobium seedling preparation: Clean the roots of healthy one-year-old Dendrobium seedlings, disinfect them, and divide them into clumps of 3-5 seedlings for later use;

[0008] (3) Dendrobium planting: First, apply a layer of protective gel to the planting window of the epiphytic tree, then place the planting base tightly on the surface of the protective gel, and finally plant the Dendrobium seedling with its roots tightly on the planting base. Wrap the roots of the Dendrobium seedling evenly with moss to ensure that the roots are completely covered by moss, and tie it firmly with straw rope to complete the Dendrobium planting.

[0009] (4) Post-planting management: Spray water every day after planting to keep the roots of Dendrobium officinale seedlings moist. After 60 days of planting, spray water only during hot summer weather to keep the roots moist. Do not spray water at other times to complete the epiphytic cultivation of Dendrobium officinale.

[0010] Furthermore, in (1), the planting points are evenly distributed in a spiral pattern between 1.2m above the ground and the first branch, with no more than 5 planting points per tree; the planting window is a rectangle with a vertical dimension of 7-8cm and a horizontal dimension of 3-4cm.

[0011] Furthermore, the disinfection method for Dendrobium officinale in (2) is: soaking in 0.01wt% potassium permanganate solution for 10-20 minutes.

[0012] Furthermore, the main raw materials of the protective gel in (3) include chitosan, ascorbic acid, and rosin, and the mass ratio of chitosan, ascorbic acid, and rosin is (5-6):0.1:(1-1.5).

[0013] Furthermore, the protective gel is prepared by dissolving chitosan in a 1 wt% acetic acid solution to obtain a 5-6 wt% chitosan solution, then adding ascorbic acid and rosin, and stirring evenly to obtain the protective gel.

[0014] Furthermore, the protective gel is applied to a thickness of 1-1.2 mm.

[0015] Furthermore, the preparation method of the planting substrate in (3) is as follows:

[0016] S1: Cut the foam ceramic board into small and large sizes that match the planting window size, soak it in a 10wt% hydrogen peroxide solution and heat it to 50-60℃ for 2 hours, take it out and wash it with clean water, and dry it in an oven at 70-80℃ for 2 hours to obtain the pretreated foam ceramic board.

[0017] S2: Dissolve 6-BA and NAA in anhydrous ethanol and add them to water. Then add soy protein and transglutaminase and stir until homogeneous to obtain a soy protein solution. Mix the soy protein solution with corn oil and stir at 40°C for 2-3 hours at a stirring speed of 500-600 rpm. After centrifugation, remove the oil phase to obtain protein microcapsules.

[0018] S3: Add potassium dihydrogen phosphate, urea, and humic acid to a 3wt% sodium alginate solution and stir to dissolve. Then add protein microcapsules and mix evenly to obtain a mixed solution. Completely immerse the pretreated foam ceramic plate in the mixed solution, let it stand for 10 minutes, and then sonicate for 3-5 minutes to obtain the planting substrate.

[0019] Furthermore, the foam ceramic board in S1 is an open-cell foam ceramic board with a porosity of 40-60%, a pore size of 4-5 mm, and a thickness of 1-2 cm.

[0020] Furthermore, the mass ratio of 6-BA, NAA, soy protein and glutaminase in S2 is 1:1:(10-12):0.02.

[0021] Furthermore, the mass ratio of soybean protein solution to corn oil in S2 is 1:2.

[0022] Furthermore, the mass ratio of sodium alginate solution, potassium dihydrogen phosphate, urea, humic acid, and protein microcapsules in S3 is 100:(3-5):(4-5):(6-8):(10-12).

[0023] This invention first removes the bark of the Magnolia grandiflora to obtain a planting window, and then plants Dendrobium nobile in the planting window. To ensure the Dendrobium nobile's independent survival later, this invention also uses a protective gel and a planting substrate. The protective gel of this invention is composed of chitosan, ascorbic acid, and rosin. In addition to preventing bark oxidation and providing a bactericidal effect, it also acts as an adhesive to bond the planting substrate to the xylem of the Magnolia grandiflora. The addition of rosin to the protective gel also improves its adhesion, thus making the initial bonding between the planting substrate and the Magnolia grandiflora trunk more secure. At the same time, the protective gel also provides a channel for the transport of substances between the planting substrate and the bark.

[0024] Foam ceramic is a lightweight, porous material. Its high porosity structure ensures unobstructed gas exchange channels in the tree trunk and provides rigid support for the roots of Dendrobium officinale. This invention uses sodium alginate gel filled into foam ceramic as a planting base for Dendrobium officinale, meeting the nutrient needs of Dendrobium officinale in the early stages of planting and promoting its growth. In the early stages, the planting base is firmly bonded to the xylem of Magnolia grandiflora by the adhesive effect of the protective gel and the binding with straw ropes. The sodium alginate gel, potassium dihydrogen phosphate, urea, and humic acid in the planting base provide moisture and nutrients to Dendrobium officinale immediately after transplanting, ensuring its survival. 6-BA and NAA are plant growth regulators that can stimulate root formation in Dendrobium orchids and induce callus formation and growth. To prevent the inhibitory effect caused by excessively high concentrations of plant growth regulators added at once, this invention uses soybean protein as the wall material to encapsulate 6-BA and NAA into protein microcapsules. Because the addition of rosin to the protective gel enhances hydrophobicity and reduces water absorption and swelling, it would hinder the release of 6-BA and NAA from the protein microcapsules. Therefore, this invention adds the protein microcapsules to the planting substrate. After Dendrobium orchid planting is completed, the protein microcapsules in the planting substrate slowly release 6-BA and NAA, which diffuse through the protective gel to the bark wound, promoting callus formation and creating a concentration gradient between the planting substrate and the bark wound. The cambium cells of the bark have a strong ability to divide. After mechanical damage and external stimulation, they can quickly divide to form callus tissue. Only a small amount of bark is removed without damaging the xylem, resulting in less damage to the Magnolia grandiflora. Under the stimulation of mechanical damage and 6-BA and NAA, the cambium cells of the bark wound rapidly divide to form callus tissue, which tends to grow in the pores of the planting substrate containing a high concentration of 6-BA and NAA. This forms a three-dimensional connection structure of xylem-callus-ceramic carrier, which firmly binds the planting substrate to the trunk. This allows the tree to provide a long-term growth site for Dendrobium without the need for tools, promoting the growth and survival of Dendrobium.

[0025] As the Dendrobium grows, the protective gel and the gel in the planting base are consumed and degraded. The roots of the Dendrobium grow and become entangled and fixed on the trunk of the Magnolia grandiflora. Nutrients accumulate in the planting base, continuously providing nutrition for the Dendrobium officinale and enabling it to survive on the Magnolia grandiflora for a long time.

[0026] Beneficial effects:

[0027] This invention provides a method for epiphytic cultivation of Dendrobium officinale on Magnolia grandiflora. The method involves removing the bark from the Magnolia grandiflora, applying a protective gel, setting up a planting base, and then planting Dendrobium officinale. This allows for rapid establishment of the Dendrobium officinale. The callus tissue of the Magnolia grandiflora is used to fix the planting base to the trunk, which can accumulate humus to continuously provide nutrition for Dendrobium officinale, enabling Dendrobium officinale to survive for a long time on the Magnolia grandiflora. Detailed Implementation

[0028] The present invention will be described in detail below with reference to specific embodiments:

[0029] The foam ceramic board used in the example is an open-cell clay foam ceramic board with a porosity of 50%, a pore size of 4-5 mm, and a thickness of 1.5 cm.

[0030] Example 1:

[0031] Preparation of protective gel:

[0032] Dissolve 5g of chitosan in a 1wt% acetic acid solution to obtain a 5wt% chitosan solution. Then add 0.1g of ascorbic acid and 1.5g of rosin and stir until homogeneous to obtain a protective gel.

[0033] Preparation of the planting substrate:

[0034] S1: Cut the foam ceramic board into small pieces of 7cm×3cm, soak them in 10wt% hydrogen peroxide solution and heat them to 50℃ for 2 hours. Take them out, wash them with clean water, and dry them in a 70℃ oven for 2 hours to obtain the pretreated foam ceramic board.

[0035] S2: Dissolve 1g 6-BA and 1g NAA in 3g anhydrous ethanol, add to 30g water, then add 12g soy protein and 0.02g transglutaminase and stir until homogeneous to obtain a soy protein solution. Mix 20g soy protein solution with 40g corn oil and stir at 500rpm at 40℃ for 3h. After centrifugation at 1500rpm / min for 2min, remove the oil phase to obtain protein microcapsules.

[0036] S3: Add 3g potassium dihydrogen phosphate, 5g urea and 8g humic acid to 100g 3wt% sodium alginate solution and stir to dissolve. Then add 10g protein microcapsules and mix evenly to obtain a mixed solution. Completely immerse the pretreated foam ceramic plate in the mixed solution, let it stand for 10 minutes and then treat it with 50KHz ultrasound for 3 minutes to obtain the planting base layer.

[0037] Example 2:

[0038] Preparation of protective gel:

[0039] Dissolve 6g of chitosan in a 1wt% acetic acid solution to obtain a 6wt% chitosan solution. Then add 0.1g of ascorbic acid and 1g of rosin and stir until homogeneous to obtain a protective gel.

[0040] Preparation of the planting substrate:

[0041] S1: Cut the foam ceramic board into small pieces of 8cm×4cm, soak them in 10wt% hydrogen peroxide solution and heat them to 60℃ for 2 hours. Take them out, wash them with water, and dry them in an 80℃ oven for 2 hours to obtain the pretreated foam ceramic board.

[0042] S2: Dissolve 1g 6-BA and 1g NAA in 3g anhydrous ethanol, add to 30g water, then add 10g soy protein and 0.02g transglutaminase and stir until homogeneous to obtain soy protein solution. Mix 20g soy protein solution with 40g corn oil and stir at 600rpm at 40℃ for 2h. After centrifugation at 1500rpm / min for 2min, remove the oil phase to obtain protein microcapsules.

[0043] S3: Add 5g potassium dihydrogen phosphate, 4g urea and 6g humic acid to 100g 3wt% sodium alginate solution and stir to dissolve. Then add 12g protein microcapsules and mix evenly to obtain a mixed solution. Completely immerse the pretreated foam ceramic plate in the mixed solution, let it stand for 10 minutes and then sonicate it at a frequency of 50KHz for 5 minutes to obtain the planting base layer.

[0044] Comparative Example 1:

[0045] Compared with the protective gel of Example 1, the only difference is that the protective gel of Comparative Example 1 does not contain rosin. The specific preparation method is as follows:

[0046] Preparation of protective gel:

[0047] Dissolve 5g of chitosan in a 1wt% acetic acid solution to obtain a 5wt% chitosan solution, then add 0.1g of ascorbic acid and stir until homogeneous to obtain a protective gel.

[0048] Comparative Example 2:

[0049] Compared with Example 1, the only difference is that the proportion of rosin added to the protective gel in Comparative Example 2 is increased. The specific preparation method is as follows:

[0050] Preparation of protective gel:

[0051] Dissolve 5g of chitosan in a 1wt% acetic acid solution to obtain a 5wt% chitosan solution. Then add 0.1g of ascorbic acid and 3g of rosin, and stir until homogeneous to obtain a protective gel.

[0052] Comparative Example 3:

[0053] Compared with the planting base layer of Example 1, the only difference in Comparative Example 2 is that protein microcapsules were not prepared. Instead, soybean protein, 6-BA, and NAA were directly mixed with sodium alginate. The specific preparation method is as follows:

[0054] Preparation of the planting substrate:

[0055] S1: Cut the foam ceramic board into small pieces of 7cm×3cm, soak them in 10wt% hydrogen peroxide solution and heat them to 50℃ for 2 hours. Take them out, wash them with clean water, and dry them in a 70℃ oven for 2 hours to obtain the pretreated foam ceramic board.

[0056] S2: Add 3g potassium dihydrogen phosphate, 5g urea, 8g humic acid, 8.6g soybean protein, 0.72g 6-BA, and 0.72g NAA to 100g of 3wt% sodium alginate solution and stir to dissolve to obtain a mixed solution. Completely immerse the pretreated foam ceramic board in the mixed solution, let it stand for 10 minutes, and then treat it with 50KHz ultrasound for 3 minutes to obtain the planting base layer.

[0057] Comparative Example 4:

[0058] Compared with the planting substrate of Example 1, the only difference is that 6-BA and NAA were directly added to the planting substrate of Comparative Example 3. The specific preparation method is as follows:

[0059] Preparation of the planting substrate:

[0060] S1: Cut the foam ceramic board into small pieces of 7cm×3cm, soak them in 10wt% hydrogen peroxide solution and heat them to 50℃ for 2 hours. Take them out, wash them with clean water, and dry them in a 70℃ oven for 2 hours to obtain the pretreated foam ceramic board.

[0061] S2: Add 3g potassium dihydrogen phosphate, 5g urea, and 8g humic acid to 100g of 3wt% sodium alginate solution and stir to dissolve. Then add 0.72g 6-BA and 0.72g NAA and mix well to obtain a mixed solution. Completely immerse the pretreated foam ceramic board in the mixed solution, let it stand for 10 minutes, and then treat it with 50KHz ultrasound for 3 minutes to obtain the planting base layer.

[0062] Example 3:

[0063] Cultivation methods of Dendrobium officinale:

[0064] (1) Epiphytic tree treatment: Select healthy Magnolia grandiflora trees with a diameter at breast height greater than 15cm and an age of less than 20 years as epiphytic trees. Determine 5 planting points between the trunk 1.2m above the ground and the first branch. The planting points are spirally distributed. Remove the bark at the planting points to obtain a rectangular planting window with a vertical dimension of 7cm and a horizontal dimension of 3cm.

[0065] (2) Dendrobium seedling preparation: Clean the roots of healthy one-year-old Dendrobium seedlings, soak them in 0.01wt% potassium permanganate solution for 20 minutes, and then divide 5 seedlings into a clump for later use;

[0066] (3) Dendrobium planting: First, apply the protective gel prepared in Example 1 to the planting window of the epiphytic tree. The thickness of the coating is 1.2 mm. Then, apply the planting base prepared in Example 1 to the surface of the protective gel. Finally, plant the Dendrobium seedlings with their roots close to the planting base. Plant one Dendrobium seedling in each planting window. Wrap the roots of the Dendrobium seedlings evenly with fresh and moist moss to ensure that the roots are completely covered by moss. Tie them firmly with straw rope to complete the Dendrobium planting.

[0067] (4) Post-planting management: Spray water every day after planting to keep the roots of Dendrobium officinale seedlings moist. After 60 days of planting, spray water only during hot summer weather to keep the roots moist. Do not spray water at other times to complete the epiphytic cultivation of Dendrobium officinale.

[0068] experiment:

[0069] In April 2023, an experiment was conducted at a Magnolia grandiflora planting base, following the above method. Healthy Magnolia grandiflora trees of uniform age and height, with a diameter at breast height of 16cm ± 0.5cm and a height from the base of the main trunk of 2.1m ± 0.1m, were selected for the epiphytic planting experiment of Dendrobium officinale. Five Magnolia grandiflora trees were randomly selected from each group for planting, and four planting sites were determined for each Magnolia grandiflora tree. The experiment was repeated three times.

[0070] Epiphytic cultivation method of Dendrobium officinale:

[0071] (1) Treatment of epiphytic trees: Four planting points are determined between the trunk 1.2m above the ground and the first branch. The planting points are evenly distributed in a spiral. The horizontal distance between adjacent planting points is 15cm and the vertical distance is 12cm. The bark of the planting points is removed to obtain a rectangular planting window with a vertical width of 7cm and a horizontal width of 3cm.

[0072] (2) Dendrobium seedling preparation: The same batch of healthy one-year-old tissue culture Dendrobium officinale were planted. The roots of the seedlings were cleaned with water, soaked in 0.01wt% potassium permanganate solution for 15 minutes, and dried. Three seedlings were divided into a clump for later use.

[0073] (3) Dendrobium planting: First, apply a 1.2mm thick protective gel to the planting window of the epiphytic tree, then attach the planting base tightly to the planting window, and finally plant the Dendrobium seedlings with their roots tightly attached to the planting base. Plant one Dendrobium seedling in each planting window, and wrap the roots of the Dendrobium seedlings evenly with fresh, moist moss to ensure that the roots are completely covered by moss, and tie them firmly with 3 straw ropes.

[0074] (4) Post-planting management: Spray water every day after planting to keep the roots of Dendrobium officinale seedlings moist. After 60 days of planting, spray water only during hot summer weather to keep the roots moist. Do not spray water at other times to complete the epiphytic cultivation of Dendrobium officinale.

[0075] 1. Experimental Group 1: The planting method described above was used, with the protective gel and planting substrate prepared in Example 1 used during the planting process;

[0076] 2. Comparative groups 1-4 adopted the planting method described in the above experiment. Comparative groups 1 and 2 used the planting substrate prepared in Example 1 and the protective gel prepared in Comparative Examples 1 and 2, respectively. Comparative groups 3 and 4 used the protective gel prepared in Example 1 and the planting substrate prepared in Comparative Examples 3 and 4, respectively.

[0077] 3. Comparative groups 5-7 adopted the planting methods described in the above experiment. In comparative group 5, the bark was not removed during the planting process, and white latex adhesive and the planting base prepared in Example 1 were used. In comparative group 6, no protective gel was used during the planting process, and only the planting base prepared in Example 1 was used. In comparative group 7, no planting base was used during the planting process, and only the protective gel prepared in Example 1 was used.

[0078] 4. The specific planting method used in the control group is as follows:

[0079] (1) Determine the planting points: Determine 4 planting points between 1.2m above the ground and the first branch of the trunk. Do not remove the bark. The planting points are evenly distributed in a spiral. The horizontal distance between adjacent planting points is 15cm and the vertical distance is 12cm.

[0080] (2) Dendrobium seedling preparation: The same batch of healthy one-year-old tissue culture Dendrobium officinale were planted. The roots of the seedlings were cleaned with water, soaked in 0.01wt% potassium permanganate solution for 15 minutes, and dried. Three seedlings were divided into a clump for later use.

[0081] (3) Dendrobium planting: Place the roots of the Dendrobium seedlings close to the planting point, wrap the roots of the Dendrobium seedlings evenly with fresh, moist moss, ensure that the roots are completely covered by moss, and tie them firmly with 3 straw ropes.

[0082] (4) Post-planting management: Spray water every day after planting to keep the roots of Dendrobium officinale seedlings moist. After 60 days of planting, spray water only during hot summer weather to keep the roots moist. Do not spray water at other times to complete the epiphytic cultivation of Dendrobium officinale.

[0083] The fall rate of the planting base layer and the survival rate of Dendrobium officinale were counted 30 days, 1 year and 2 years after the completion of Dendrobium officinale planting. The results are shown in Table 1.

[0084] Planting substrate drop rate (%) = Number of fallen planting substrate pieces / Number of planting substrate pieces used × 100%.

[0085] Survival rate of Dendrobium officinale (%) = Number of surviving Dendrobium officinale / Number of Dendrobium officinale plants × 100%.

[0086] Table 1

[0087]

[0088] Analysis of the data in Table 1 shows that:

[0089] 1. The Dendrobium officinale planted in experimental group 1 can quickly establish itself and survive on the Magnolia grandiflora. The planting base is fixed on the trunk of the Magnolia grandiflora and can provide nutrients for Dendrobium officinale for a long time, resulting in a high survival rate of Dendrobium officinale.

[0090] 2. Compared with experimental group 1, the protective gels used in control groups 1 and 2 were different. The protective gel used in control group 1 did not contain rosin, resulting in a decrease in the antibacterial effect and adhesive properties of the protective gel. In the early stage, the bond between the planting base and the epiphytic tree was not strong. When the binding straw rope loosened, the planting base was easy to fall off, and Dendrobium officinale lacked a source of nutrients. Therefore, the survival rate of Dendrobium officinale decreased after 1 and 2 years of planting. The protective gel used in control group 2 had an increased rosin content, which improved the strength of the protective gel and enhanced its hydrophobic effect. 6-BA and NAA diffused slowly in the protective gel and could not quickly diffuse to the trunk wound to promote callus growth. At the same time, the increased strength of the protective gel hindered callus growth, making it difficult for the bark callus to grow into the pores of the planting base. Therefore, the fixation effect on the planting base decreased. After a long time, the straw rope rotted and loosened, the planting base fell off, and Dendrobium officinale could not survive independently, thus reducing the survival rate.

[0091] 3. Compared with experimental group 1, the preparation of planting substrate in control groups 3 and 4 was different. Control group 3 did not prepare microcapsules, but added soy protein, 6-BA and NAA directly to the planting substrate. Control group 4 did not use soy protein, but added 6-BA and NAA directly to the planting substrate. The high concentration of plant growth regulators in the planting substrate inhibited the production of new roots of Dendrobium officinale and inhibited the growth of bark callus tissue, resulting in weaker Dendrobium officinale vitality and difficulty for the callus tissue of the epiphytic tree to grow into the pores of the planting substrate to fix the planting substrate. As a result, the survival rate of Dendrobium officinale gradually decreased over time.

[0092] 4. Compared with experimental group 1, the planting methods of control groups 5-7 were different. In control group 5, the bark was not removed, so callus tissue was not formed to fix the planting base. After a long period of planting, the adhesive white latex weathered, the binding straw rope loosened and rotted, and the planting base was easy to fall off, affecting the survival of Dendrobium officinale. In control group 6, no protective gel was used. In the early stage, the firmness of the connection between the planting base and the epiphytic tree decreased. The bark wound was easily infected by pathogens and oxidative stress, which affected the formation of callus tissue. As a result, the planting base was not firmly connected to the trunk and was easy to fall off. Therefore, the survival rate of Dendrobium officinale gradually decreased. At the same time, pathogens from the bark wound could easily infect the Dendrobium officinale seedlings and reduce the survival rate of Dendrobium officinale. In control group 7, no planting base was used. After a long period of time, the bark wound healed, the protective gel was degraded, and the moss at the base of the Dendrobium officinale fell off. The Dendrobium officinale did not absorb enough nutrients and gradually died, and the survival rate decreased year by year.

[0093] 5. In the control group, Dendrobium officinale was wrapped in moss and tied to Magnolia grandiflora. The smooth bark of Magnolia grandiflora could not accumulate humus and water to promote the survival of Dendrobium officinale. When the moss fell off, Dendrobium officinale gradually died, and the survival rate decreased.

[0094] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit it. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the spirit and scope of the present invention, and all such modifications or substitutions should be covered within the scope of the claims of the present invention.

Claims

1. A method for epiphytic cultivation of Dendrobium officinale on Magnolia grandiflora, characterized in that, The method is as follows: (1) Treatment of epiphytic trees: Select healthy magnolia trees with a diameter at breast height greater than 15cm as epiphytic trees, determine the planting point on the trunk, and remove the bark at the planting point to the depth of just touching the xylem to obtain the planting window; (2) Dendrobium seedling preparation: Clean the roots of healthy one-year-old Dendrobium seedlings, disinfect them, and divide them into clumps of 3-5 seedlings for later use; (3) Dendrobium planting: First, apply a layer of protective gel to the planting window of the epiphytic tree, then place the planting base tightly on the surface of the protective gel, and finally place the roots of the Dendrobium seedlings tightly on the planting base. Wrap the roots of the Dendrobium seedlings evenly with moss to ensure that the roots are completely covered by moss, and tie them firmly with straw rope to complete the Dendrobium planting. (4) Post-planting management: Spray water daily to keep the roots of Dendrobium officinale seedlings moist. After 60 days of planting, spray water only during hot summer weather to keep the roots moist. Do not spray water at other times to complete the epiphytic cultivation of Dendrobium officinale. The main raw materials of the protective gel in step (3) include chitosan, ascorbic acid, and rosin, and the mass ratio of chitosan, ascorbic acid, and rosin is (5-6):0.1:(1-1.5); the coating thickness of the protective gel is 1-1.2 mm. The preparation method of the planting substrate in step (3) is as follows: S1: Cut the foam ceramic board into small and large sizes that match the planting window size, soak it in a 10wt% hydrogen peroxide solution and heat it to 50-60℃ for 2 hours, take it out and wash it with clean water, and dry it in an oven at 70-80℃ for 2 hours to obtain the pretreated foam ceramic board. S2: Dissolve 6-BA and NAA in anhydrous ethanol and add them to water. Then add soy protein and transglutaminase and stir until homogeneous to obtain a soy protein solution. Mix the soy protein solution with corn oil and stir at 40°C for 2-3 hours at a stirring speed of 500-600 rpm. After centrifugation, remove the oil phase to obtain protein microcapsules. S3: Add potassium dihydrogen phosphate, urea, and humic acid to a 3wt% sodium alginate solution and stir to dissolve. Then add protein microcapsules and mix evenly to obtain a mixed solution. Completely immerse the pretreated foam ceramic plate in the mixed solution, let it stand for 10 minutes, and then sonicate for 3-5 minutes to obtain the planting substrate.

2. The method for epiphytic cultivation of Dendrobium officinale on Magnolia grandiflora according to claim 1, characterized in that, In step (1), the planting points are evenly distributed in a spiral pattern between 1.2m above the ground on the trunk and the first branch; the planting window is a rectangle with a vertical dimension of 7-8cm and a horizontal dimension of 3-4cm.

3. The method for epiphytic cultivation of Dendrobium officinale on Magnolia grandiflora according to claim 2, characterized in that, The protective gel is prepared by dissolving chitosan in a 1 wt% acetic acid solution to obtain a 5-6 wt% chitosan solution, then adding ascorbic acid and rosin, and stirring until uniform to obtain the protective gel.

4. A method for epiphytic cultivation of Dendrobium officinale on Magnolia grandiflora according to claim 3, characterized in that, In step S1, the foam ceramic board is an open-cell foam ceramic board with a porosity of 40-60%, a pore size of 4-5 mm, and a thickness of 1-2 cm.

5. A method for epiphytic cultivation of Dendrobium officinale on Magnolia grandiflora according to claim 4, characterized in that, In step S2, the mass ratio of 6-BA, NAA, soy protein, and transglutaminase is 1:1:(10-12):0.02; the mass ratio of the soy protein solution to corn oil is 1:

2.

6. A method for epiphytic cultivation of Dendrobium officinale on Magnolia grandiflora according to claim 5, characterized in that, In step S3, the mass ratio of sodium alginate solution, potassium dihydrogen phosphate, urea, humic acid, and protein microcapsules is 100:(3-5):(4-5):(6-8):(10-12).

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